Abstract:
:Although the microscopic examination of stool samples remains the reference method of choice for the diagnosis of intestinal protistan infections, this method is time-consuming and requires experienced and well-trained operators. The purpose of this study was to evaluate the level of agreement between the BD MAX TM Enteric Parasite Panel (EPP) and microscopy for the detection of Giardia intestinalis (Lambl, 1859), Cryptosporidium spp. and Entamoeba histolytica Schaudinn, 1903 in stool samples. The study included faecal samples of 362 patients who were admitted to our hospital due to gastrointestinal complaints. In the microscopic examination, which was made with the native-lugol method on the stool samples that were taken from the patients, cysts, trophozoites and eggs of the parasite were examined. The diagnosis of G. intestinalis, Cryptosporidium parvum Tyzzer, 1912 and Cryptosporidium hominis Morgan-Ryan, Fall, Ward, Hijjawi, Sulaiman, Fayer, Thompson, Olson, Lal et Xiao, 2002, and E. histolytica was made in the faecal samples using the EPP assay. In the microscopic examination, Cryptosporidium spp. positive stool samples were stained with kinyoun's acid-fast. In the microscopic examination, parasites were detected in 41 (11%) of the 362 stool samples. In contrast, EPP assay identified parasites in 23 (6.3%) of the samples. In the microscopic examination, E. histolytica and Entamoeba dispar Brumpt, 1925 were detected in 22 (6.1%) of the samples, G. intestinalis was seen in 15 (4.1%), and C. parvum or C. hominis were detected in three (0.8%); these values were five (1.4%), 16 (4.4%) and two (0.5%) positive with the EPP assay. Although C. parvum or C. hominis were detected as positive in the microscopic examination of three samples, only two of the samples were positive in both EPP assay and kinyoun's acid-fast method. The EPP assay is a relatively simple test that can distinguish E. histolytica and E. dispar, but it cannot replace microscopy in the diagnosis of amoebiasis. Diagnosis for G. intestinalis and C. parvum/C. hominis with the BD MAXTM enteric parasite panel was equivalent to that with microscopy. We believe that E. histolytica must be diagnosed with nucleic acid amplification tests that have a high sensitivity and specificity like EPP assay in certain patient groups.
journal_name
Folia Parasitol (Praha)journal_title
Folia parasitologicaauthors
Akgun S,Celik Tdoi
10.14411/fp.2020.020subject
Has Abstractpub_date
2020-08-07 00:00:00eissn
0015-5683issn
1803-6465journal_volume
67pub_type
杂志文章abstract::While studying the suslik fleas in artificial nests planted in free nature, significant changes in the population dynamics of N. setosa, C. simplex and C. orientalis (from the spring build up to the autumnal peak and the decrease of flea number until the next spring) were noted. The predominance of females in the spri...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::Frezella gen. n. is proposed to accommodate Frezella vaucheri sp. n. from poorly known auchenipterid fish, Tocantinsia piresi (Miranda Ribeiro), from the Xingú River, one of the principal tributaries of the lower Amazon River in Brazil. The new genus belongs to the Proteocephalinae because of the medullary position of...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2015.006
更新日期:2015-01-01 00:00:00
abstract::Philometra overstreeti Moravec et de Buron, 2006 and Philomnetroides paralichthydis Moravec et de Buron, 2006 are common parasites of the southern flounder, Paralichthys lethostigma Jordan et Gilbert. Because the life cycles of these parasites are unknown, our goal was to assess whether species of copepod commonly fou...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2008.040
更新日期:2008-12-01 00:00:00
abstract::A relatively high rate of mortality among engorged females of Ornithodoros moubata (Murray, 1877) was observed in our laboratory colony. The general aim of the study was to identify the causative agent responsible for this mortality. The diagnostic tests were performed by Yeast Identification Service (CBS-Delft, Nethe...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::This work describes the ecological characteristics of the intestinal helminth communities of 50 wolves (Canis lupus L.) from Spain. The species found were classified into three groups according to prevalence, intensity and intestinal distribution. Taenia hydatigena Pallas, 1766 and Uncinaria stenocephala (Railliet, 18...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2003.041
更新日期:2003-09-01 00:00:00
abstract::Twenty two percent (22/98) of intertidal fishes of 10 species captured in South Africa at Koppie Alleen, De Hoop Nature Reserve (south coast) and Mouille Point, Cape Town (west coast), harboured single or combined infections of haemogregarines, trypanosomes and an intraerythrocytic parasite resembling a Haemohormidium...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2006.031
更新日期:2006-12-01 00:00:00
abstract::Sex ratio and engorged females ratio were studied in the main mosquito species populations (Aedes aegypti, Culex quinquefasciatus and partially Anopheles vagus) in the houses of Phnom Penh. Collections were carried out in 7 different localities, including area from the centre of the town to the periphery. In Ae. aegyp...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::The cestode fauna of the darkspotted numbfish, Narcine maculata (Shaw) (Torpediniformes: Narcinidae), from Malaysian Bomrneo was examined for the first time. This work resulted in the discovery of a new genus and two new species of Anteroporidae (Lecanicephalidea). Sesquipedalapex comicus gen. n., sp. n. was erected o...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2011.010
更新日期:2011-06-01 00:00:00
abstract::Four laboratory-hatched European kestrels Falco tinnunculus L. were fed on laboratory mice and common voles Microtus arvalis Pallas previously inoculated with different doses of sporulated oocysts of Caryospora kutzeri Böer, 1982. Two kestrels that were fed infected mice shed C. kurtzeri oocysts 6 days after ingesting...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2001.003
更新日期:2001-01-01 00:00:00
abstract::Two squirrels aged 16 weeks and three muskrats aged 24 weeks were subcutaneously infected with a dose of 400 SMicLD50 of the extraneurally passaged "236" strain of Tahyna virus. Viremia was detected in one squirrel (48 and 96 hours post infection) and in two muskrats (24 and 48 hours p.i.). Seroconversion was demonstr...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::Two species of Coccomyxa Léger et Hesse, 1907, one of the least studied myxosporean genera, are reported from shallow coastal waters in the Gulf of Eilat, Red Sea, Israel. A new species, Coccomyxajirilomi sp. n. is described from the spotted frillgoby Bathygobius cyclopterus (Valenciennes) (Gobiidae). It forms polyspo...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2007.016
更新日期:2007-06-01 00:00:00
abstract::The philometrid nematode, Philometra saltatrix Ramachandran, 1973, is redescribed from male and female specimens collected from the ovaries of the marine fish (bluefish) Pomatomus saltatrix (L.) (Pomatomidae, Perciformes) from the Tuscan Sea, Italy. The light and scanning electron microscopical examination made it pos...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2008.029
更新日期:2008-09-01 00:00:00
abstract::The structure of legs I and of gnathosoma in Radfordia lemnina (Koch, 1835) and Protomyobia claparedei (Poppe, 1896) is described on the basis of SEM micrographs. ...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::The chemoprophylactic and chemotherapeutic effects of levamisole and mebendazole on Angiostrongylus malaysiensis infection in rats were studied. Both drugs were ineffective in preventing infection while the post-infection treatment showed 100% efficacy. Furthermore, levamisole and mebendazole when given in combination...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Investigations on the epizoic fauna of Gadus morhua (L.), Platichthys flesus (L.) and Oncorhynchus mykiss (Walbaum) from the Kiel Fjord and Kiel Bight were carried out from September 1996 to March 1997. Smears from 120 G. morhua and 92 P. flesus caught using fish traps and trammel nets, and of 35 O. mykiss obtained fr...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2000.018
更新日期:2000-01-01 00:00:00
abstract::Polymorphic microsatellite loci were characterised for two louse species, the anopluran Polyplax serrata Burmeister, 1839, parasitising Eurasian field mice of the genus Apodemus Kaup, and the amblyceran Myrsidea nesomimi Palma et Price, 2010, found on mocking birds endemic to the Galápagos Islands. Evolutionary histor...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2015.016
更新日期:2015-01-01 00:00:00
abstract::The effects of condensed tannins (CTs) extracted from five species of plants on egg hatching and larval development of Teladorsagia circumcincta (Stadelmann, 1894) (syn. Ostertagia circumcincta) were evaluated using in vitro bioassays. The extracts of CTs were obtained from Lotus pedunculatus (LP), Lotus corniculatus ...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2010.008
更新日期:2010-03-01 00:00:00
abstract::Arostrilepis beringiensis (Kontrimavichus et Smirnova, 1991) is redescribed on the basis of its type specimens from Lemmus trimucronatus (Richardson) and material from the collections of the Institute of Systematics and Ecology of Animals, Novosibirsk from the lemmings Myopus schisticolor (Lilljeborg) and Lemmus sibir...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2011.029
更新日期:2011-11-01 00:00:00
abstract::Based on light and scanning electron microscopical studies, the following five gonad-infecting species of the Philometridae (Nematoda: Dracunculoidea) are described from marine perciform fishes off the eastern coast of India (Bay of Bengal): Philometra sphyraenae sp. n. (males and females) from the pickhandle barracud...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2013.012
更新日期:2013-05-01 00:00:00
abstract::Physaloptera brevivaginata has been found parasitising the stomach of two species of bats of the family Vespertilionidae, Myotis myotis and Myotis blythii, in Spain. A comparative study of the prevalences and mean intensities of parasitism by this physalopterid revealed no statistically significant differences between...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::The effects of infections of Trichinella spiralis on 10 specific-pathogen-free Beagles were examined. Eight puppies received either 100, 500, 1,000, or 5,000 larvae, and 2 adult dogs received 1,000 larvae. Blood was drawn every 4 days, beginning 5 days before infection, for the determination of relative eosinophil num...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::This paper deals with some ecology and evolution problems of monogeneans--gill parasites of fishes, such as congeneric simultaneous doublets, spatial distribution of species on the gills, interspecific restrictions of biotopes etc. The author proves competitive character of site selection in monogeneans. The role of h...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::The aim of the present study was to estimate the genetic diversity of the cestode Echinococcus multilocularis Leuckart, 1863 in Poland based on sequence analysis of the mitochondrial genes of worms isolated from red foxes, Vulpes vulpes (Linnaeus). Overall, 83 adults of E. multilocularis from the same number of foxes ...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2017.007
更新日期:2017-03-09 00:00:00
abstract::Soricinia tripartita Zarnowski, 1955 is redescribed on the basis of specimens from the type host Sorex araneus Linnaeus from Lithuania, Latvia and Russia (Republic of Karelia and Republic of Komi - a new geographical record) as well as from Sorex satunini Ognev and Sorex volnuchini Ognev from Russia (Nalchik Area in t...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:2014-04-01 00:00:00
abstract::The course of penetration of the ovicidal fungus Verticillium chlamydosporium Goddard through the Ascaris lumbricoides egg-shells was studied in electron miorographs. The contact area between the egg and the fungus is smooth at the stage of the contact of the ovicidal fungus with the egg surface, as well as at the sta...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract::A new genus of the family Capillariidae, Tridentocapillaria gen. n., has been established. A type species is Tridentocapillaria tridens (Dujardin, 1845) comb. n. Other species of this genus are T. eurycerca (Oschmarin et Parukhin, 1963) comb. n., T. parusi (Wakelin, Schmidt et Kuntz, 1970) comb. n., and T. javanensis ...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Faecal samples from 162 wild animals were collected from 32 distinct sites of Łęczyńsko-Włodawskie Lakeland (eastern Poland). The presence of Giardia duodenalis (Stiles, 1902) was assessed by a Direct Fluorescence Assay (DFA) and by Polymerase Chain Reaction (PCR) and sequencing of a fragment of the beta-giardin gene....
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:10.14411/fp.2015.042
更新日期:2015-07-30 00:00:00
abstract::Plasmodia of a Henneguya species measuring 70-900 microns and exhibiting season-dependent stages of development were detected throughout a three-year study on gill myxosporosis of Lake Balaton pikeperch (Stizostedion lucioperca (L.)). Sixty-five out of 160 fish (41%) examined in the period of study were infected by th...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::The developmental cycle of the trematode Rubenstrema exasperatum (Rudolphi, 1819), a common parasite of insectivores of the family Soricidae, is described here for the first time. The first intermediate host in nature is the snail Planorbarius corneus. Metacercariae encysted experimentally in larvae of the mosquito Cu...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::From October 1985 until July 1987, the bionomy of the trematode Allocreadium isoporum (Looss, 1894) was studied in the Rokytná River (the Danube basin), Czechoslovakia. While clams of the genera Sphaerium Scopoli and Pisidium Pfeiffer apparently serve as its first intermediate hosts, mayfly larvae Ephemera danica Müll...
journal_title:Folia parasitologica
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00