Abstract:
:The difference in DNA content of peripheral lymphocytes from normal males, normal females, and an individual with a 48 (xxxy) chromosome constitution was determined by rapid flow microfluorometric techniques. A similar comparison was performed using tissue culture fibroblasts derived from an individual with a 49 (xxxxy) chromosome constitution and WI-38 cells as a normal control. Less than 60 min were required to isolate the lymphocytes, to stain the cells fluorescently, and to measure the increased DNA content. The measured increase in DNA content is consistent with chromosome DNA analyses and chromosome length measurements.
journal_name
Hum Genetjournal_title
Human geneticsauthors
Cram LS,Lehman JMdoi
10.1007/BF00393583subject
Has Abstractpub_date
1977-06-30 00:00:00pages
201-6issue
2eissn
0340-6717issn
1432-1203journal_volume
37pub_type
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