Low expression of the Lsp1 gene in early mouse T-lymphomas induced by N-methyl-N-nitrosourea.


:The mouse Lsp1 gene encodes a 330 amino acid intracellular F-actin binding protein. Previously we showed that the mouse and human Lsp1 genes are expressed in normal B-cells and T-cells, including Thy1+ thymocytes and in normal macrophages and neutrophils. No or little LSP1 RNA and protein was found in a series of transformed mouse and human T-lymphoma cell lines, although normal antigen and lymphokine dependent T-cell lines expressed the Lsp1 gene. Here we show by Northern analysis that three mature antigen independent T-cell lines (CTLL-2, HT-2 and 532.10) which grow in the presence of lymphokine only, do not express LSP1 RNA, while mature resting and activated lymph node T-cells express high levels of LSP1 RNA. To determine whether the down-regulation of LSP1 expression is an early event which occurs in vivo in the tumor, rather than as a consequence of in vitro propagation of T-lymphoma cells, we analyzed LSP1 expression in primary T-lymphomas induced in AKR/J or AKR/J x BALB/cJ mice by a single intraperitoneal (i.p.) injection with 75 mg N-methyl-N-nitrosourea (MNU) per kg body-weight. Two- color Fluorescence Activated Cell Sorter (FACS) analysis showed that all tumors had an immature CD4+/CD8+ double positive (DP) phenotype. Many tumors contained a substantial population of CD4+ single positive (SP) cells. Since these cells may be infiltrating lymphocytes which can be expected to express the Lsp1 gene at a high level these tumors were not included in our analysis. Nineteen tumors were analyzed for Lsp1 gene expression and 13 contained reduced levels of LSP1 RNA, ranging from 4% to 44% of those found in age-matched normal thymus. Six tumors showed either no or only a small reduction in LSP1 RNA. These tumors had developed later than those expressing low levels of LSP1. The level of LSP1 RNA in tumors developing <110 days after injection of MNU was 19.1% +/- 5.2 (mean +/- SEM), while the level of LSP1 in later tumors was 78.4% +/- 13.0 (P = 0.004). Similar data were obtained when the expression of LSP1 protein was analyzed. These findings extend our previous data in several ways. First, they suggest a correlation between the down-regulation of LSP1 expression and abnormal regulation of growth or survival of immature and mature T-lymphocytes. Second, they show that down-regulation of the Lsp1 gene in transformed T-cells is not the result of prolonged in vitro culture, but occurs in the majority of primary tumors. Third, they show that there are two classes of T-lymphoma, which differ in their expression of LSP1 RNA and that the down-regulation of LSP1 is specifically associated with the early appearance of T-lymphoma after injection with MNU. This strongly suggests that the absence or reduced expression of LSP1 contributed to the transformation process and argues against the possibility that loss of LSP1 expression is a mere inconsequential epigenetic event.






Brennan L,Jongstra J




Has Abstract


1996-04-01 00:00:00












  • Biomarkers of styrene exposure in lamination workers: levels of O6-guanine DNA adducts, DNA strand breaks and mutant frequencies in the hypoxanthine guanine phosphoribosyltransferase gene in T-lymphocytes.

    abstract::Occupational exposure to styrene was studied in nine workers of a hand lamination plant in Bohemia. Personal dosimeters were used to monitor the styrene workplace exposure, and the levels of styrene in blood and mandelic acid in urine were measured. Blood samples were taken at four occasions during a 7 month period to...


    pub_type: 杂志文章


    authors: Vodicka P,Bastlová T,Vodicková L,Peterková K,Lambert B,Hemminki K

    更新日期:1995-07-01 00:00:00

  • Inducible COX-2-dependent apoptosis in human ovarian cancer cells.

    abstract::Resveratrol is a naturally occurring trihydroxyl-diphenylethylene compound that has been shown experimentally to have beneficial effects in the treatment of cancer and cardiovascular disease. Resveratrol induces programmed cell death (apoptosis) in these cells and activates important signal transducing proteins includ...


    pub_type: 杂志文章


    authors: Lin C,Crawford DR,Lin S,Hwang J,Sebuyira A,Meng R,Westfall JE,Tang HY,Lin S,Yu PY,Davis PJ,Lin HY

    更新日期:2011-01-01 00:00:00

  • The principal urinary metabolite of allyl isothiocyanate, N-acetyl-S-(N-allylthiocarbamoyl)cysteine, inhibits the growth and muscle invasion of bladder cancer.

    abstract::Naturally occurring allyl isothiocyanate (AITC) was recently shown to be selectively delivered to bladder cancer tissue via urinary excretion and to inhibit bladder cancer growth and muscle invasion in an animal model. AITC is excreted in urine mainly as N-acetyl-S-(N-allylthiocarbamoyl)cysteine, more commonly known a...


    pub_type: 杂志文章


    authors: Bhattacharya A,Li Y,Geng F,Munday R,Zhang Y

    更新日期:2012-02-01 00:00:00

  • Quandtitative correlation among DNA damaging potency of six N-nitroso compounds and their potency in inducing tumor growth an bacterial mutations.

    abstract::Six N-nitroso compounds (N-nitrosodimethylamine, N-nitrosodiethylamine, N-nitrosodi-n-propylamine, N-nitroso-N-methylurea, N-nitrosopyrrolidine, and N-nitrosodi-n-butylamine) were tested in rats for liver DNA damaging activity by the alkaline elution technique. On molar basis their DNA damaging potency was found to de...


    pub_type: 杂志文章


    authors: Brambilla G,Cavanna M,Pino A,Robbiano L

    更新日期:1981-01-01 00:00:00

  • Endocrine dysfunction in p27Kip1 deficient mice and susceptibility to Wnt-1 driven breast cancer.

    abstract::The cyclin-dependent kinase (Cdk) inhibitor p27(Kip1) (p27) is a marker of prognosis in many cancers, including breast cancer. Low p27 expression correlates with poor prognosis, especially in hormone receptor positive breast tumors. This association suggests a role for p27 in hormone-dependent cancer. We used the Wnt-...


    pub_type: 杂志文章


    authors: Glover CE,Gurley KE,Kim KH,Storer B,Fero ML,Kemp CJ

    更新日期:2009-06-01 00:00:00

  • Influence of GSTM1, GSTT1, GSTP1, NAT1, NAT2, EPHX1, MTR and MTHFR polymorphism on chromosomal aberration frequencies in human lymphocytes.

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    pub_type: 杂志文章


    authors: Skjelbred CF,Svendsen M,Haugan V,Eek AK,Clausen KO,Kure EH,Tuimala JT,Svendsen MV,Norppa H,Hansteen IL

    更新日期:2011-03-01 00:00:00

  • Biochemical characteristics of endonuclease activity within the nucleotidyl DNA excision repair pathway of permeable human fibroblasts.

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    pub_type: 杂志文章


    authors: Kaufmann WK,Briley LP

    更新日期:1990-01-01 00:00:00

  • A novel experimental model for human mixed acinar-ductal pancreatic cancer.

    abstract::Pancreatic cancer has remained refractory to treatment. In large part, this results from the lack of an animal model that mimics pancreatic cancer in man. We describe a novel experimental model of pancreatic cancer that shares the genetic background, histologic features and natural history of human mixed acinar-ductal...


    pub_type: 杂志文章


    authors: Doiron B,DeFronzo RA

    更新日期:2018-02-09 00:00:00

  • 3,3-Diindolylmethane (DIM): a nutritional intervention and its impact on breast density in healthy BRCA carriers. A prospective clinical trial.

    abstract::Women who carry the BRCA mutation are at high lifetime risk of breast cancer, but there is no consensus regarding an effective and safe chemoprevention strategy. A large body of evidence suggests that 3,3-diindolylmethane (DIM), a dimer of indole-3-carbinol found in cruciferous vegetables, can potentially prevent carc...


    pub_type: 杂志文章


    authors: Yerushalmi R,Bargil S,Ber Y,Ozlavo R,Sivan T,Rapson Y,Pomerantz A,Tsoref D,Sharon E,Caspi O,Grubsrein A,Margel D

    更新日期:2020-10-15 00:00:00

  • Effects of glucose on the formation of PhIP in a model system.

    abstract::The effect of glucose on the formation of the food mutagen PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) was studied in a model system. When a mixture of creatine (0.9 mmol), phenylalanine (0.9 mmol) and glucose (0.45 mmol) was heated in diethylene glycol and water (3 ml, 5:1) for 10 min at 180 or 225 degrees...


    pub_type: 杂志文章


    authors: Skog K,Jägerstad M

    更新日期:1991-12-01 00:00:00

  • Comparative benefits of Nab-paclitaxel over gemcitabine or polysorbate-based docetaxel in experimental pancreatic cancer.

    abstract::Gemcitabine has limited clinical benefits in pancreatic ductal adenocarcinoma. The solvent-based traditional taxanes docetaxel and paclitaxel have not shown clinical results superior to gemcitabine. Nab-paclitaxel, a water-soluble albumin-bound paclitaxel, may carry superior distribution properties into the tumor micr...


    pub_type: 杂志文章


    authors: Awasthi N,Zhang C,Schwarz AM,Hinz S,Wang C,Williams NS,Schwarz MA,Schwarz RE

    更新日期:2013-10-01 00:00:00

  • lncMat2B regulated by severe hypoxia induces cisplatin resistance by increasing DNA damage repair and tumor-initiating population in breast cancer cells.

    abstract::Multicellular tumor spheroids (MCTSs) constitute a three-dimensional culture system that recapitulates the in vivo tumor microenvironment. Tumor cells cultured as MCTSs present antineoplastic resistance due to the effect of microenvironmental signals acting upon them. In this work, we evaluated the biological function...


    pub_type: 杂志文章


    authors: García-Venzor A,Mandujano-Tinoco EA,Ruiz-Silvestre A,Sánchez JM,Lizarraga F,Zampedri C,Melendez-Zajgla J,Maldonado V

    更新日期:2020-11-13 00:00:00

  • Detection of N2,3-ethanoguanine in DNA after treatment with chloroacetaldehyde in vitro.

    abstract::The reaction of chloroacetaldehyde, a reactive metabolic of the carcinogen vinyl chloride, with DNA produces in addition to the hitherto known adducts, 1,N6-ethenoadenine and 3,N4-ethenocytosine, an ethenoguanine adduct, namely N2,3-ethenoguanine. This adduct is formed in the reaction of chloroacetaldehyde with the fr...


    pub_type: 杂志文章


    authors: Oesch F,Doerjer G

    更新日期:1982-01-01 00:00:00

  • Epigenetic therapy using the histone deacetylase inhibitor for increasing therapeutic gain in oral cancer: prevention of radiation-induced oral mucositis and inhibition of chemical-induced oral carcinogenesis.

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    pub_type: 杂志文章


    authors: Chung YL,Lee MY,Pui NN

    更新日期:2009-08-01 00:00:00

  • Non-mutagenic carcinogens are primarily hydrophobic.

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    pub_type: 杂志文章


    authors: McCoy GD,Rosenkranz HS,Klopman G

    更新日期:1990-07-01 00:00:00

  • An insulin-like growth factor-II intronic variant affects local DNA conformation and ovarian cancer survival.

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    pub_type: 杂志文章


    authors: Lu L,Risch E,Deng Q,Biglia N,Picardo E,Katsaros D,Yu H

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  • Interactions among GSTM1, GSTT1 and GSTP1 polymorphisms, cruciferous vegetable intake and breast cancer risk.

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    authors: Steck SE,Gaudet MM,Britton JA,Teitelbaum SL,Terry MB,Neugut AI,Santella RM,Gammon MD

    更新日期:2007-09-01 00:00:00

  • Differential transcriptional regulation of human telomerase in a cellular model representing important genetic alterations in esophageal squamous carcinogenesis.

    abstract::Telomerase activity is observed in approximately 90% of human cancer including esophageal squamous cell cancer. Normal somatic cells do not display telomerase activity on a regular basis. The major mechanism to regulate telomerase activity in human cells is the transcriptional control of the catalytic subunit, the hum...


    pub_type: 杂志文章


    authors: Quante M,Heeg S,von Werder A,Goessel G,Fulda C,Doebele M,Nakagawa H,Beijersbergen R,Blum HE,Opitz OG

    更新日期:2005-11-01 00:00:00

  • Tissue protein turnover during liver carcinogenesis.

    abstract::Overall rates of tissue protein degradation in vivo during chemical hepatocarcinogenesis were estimated by a double-isotope method as well as from the accumulation of peptide intermediates in protein degradation induced by bestatin. Several parameters estimating rates of cell proliferation and cell loss have been meas...


    pub_type: 杂志文章


    authors: Canuto RA,Tessitore L,Muzio G,Autelli R,Baccino FM

    更新日期:1993-12-01 00:00:00

  • Regulation of ornithine decarboxylase gene expression in normal and transformed hamster embryo fibroblasts following stimulation by 12-O-tetradecanoylphorbol-13-acetate.

    abstract::We have compared the regulation of ornithine decarboxylase (ODC) gene expression in primary cultures of hamster embryo fibroblasts and in two independently transformed hamster embryo cell lines. Previous studies have demonstrated that 12-O-tetradecanoylphorbol-13-acetate (TPA) can greatly potentiate the serum growth f...


    pub_type: 杂志文章


    authors: Gilmour SK,O'Brien TG

    更新日期:1989-01-01 00:00:00

  • Grainyhead-like 2 regulates epithelial plasticity and stemness in oral cancer cells.

    abstract::Grainyhead-like 2 (GRHL2) is one of the three mammalian homologues of Drosophila Grainyhead involved in epithelial morphogenesis. We recently showed that GRHL2 also controls normal epithelial cell proliferation and differentiation. In this study, we investigated the role of GRHL2 in oral carcinogenesis and the underly...


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    authors: Chen W,Yi JK,Shimane T,Mehrazarin S,Lin YL,Shin KH,Kim RH,Park NH,Kang MK

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  • The O-methylation of 4-hydroxyestradiol is inhibited by 2-hydroxyestradiol: implications for estrogen-induced carcinogenesis.

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    pub_type: 杂志文章


    authors: Roy D,Weisz J,Liehr JG

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  • Sulforaphane inhibits histone deacetylase activity in BPH-1, LnCaP and PC-3 prostate epithelial cells.

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    authors: Myzak MC,Hardin K,Wang R,Dashwood RH,Ho E

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  • A 5-microRNA signature identified from serum microRNA profiling predicts survival in patients with advanced stage non-small cell lung cancer.

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  • Effect of tumour promoters and non-genotoxic carcinogens on terminal differentiation and proliferation in mouse teratoma XB2 cells cultured in low calcium medium.

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    pub_type: 杂志文章


    authors: Hewitt SD,Duffy PA,Orton TC,Garner RC

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  • Deficiency of C-C chemokine receptor 5 suppresses tumor development via inactivation of NF-κB and inhibition of monocyte chemoattractant protein-1 in urethane-induced lung tumor model.

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  • Pretreatment of normal human fibroblasts and human colon carcinoma cells with MNNG allows chloroethylnitrosourea to produce DNA interstrand crosslinks not observed in cells treated with chloroethylnitrosourea alone.

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    pub_type: 杂志文章


    authors: Zlotogorski C,Erickson LC

    更新日期:1983-01-01 00:00:00

  • Differential effects of resveratrol on androgen-responsive LNCaP human prostate cancer cells in vitro and in vivo.

    abstract::Resveratrol is a phytochemical that has been under consideration for use as a prostate cancer chemopreventive agent. However, the efficacy, as well as the mechanisms of action of resveratrol on prostate cancer prevention, remains largely unknown. This study seeks to address these questions and examine the cancer preve...


    pub_type: 杂志文章


    authors: Wang TT,Hudson TS,Wang TC,Remsberg CM,Davies NM,Takahashi Y,Kim YS,Seifried H,Vinyard BT,Perkins SN,Hursting SD

    更新日期:2008-10-01 00:00:00

  • Detection of CYP1A1 protein in human liver and induction by TCDD in precision-cut liver slices incubated in dynamic organ culture.

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    pub_type: 杂志文章


    authors: Drahushuk AT,McGarrigle BP,Larsen KE,Stegeman JJ,Olson JR

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  • Multidirectional tumor-suppressive activity of AIMP2/p38 and the enhanced susceptibility of AIMP2 heterozygous mice to carcinogenesis.

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    pub_type: 杂志文章


    authors: Choi JW,Um JY,Kundu JK,Surh YJ,Kim S

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