Abstract：
:Pancreatic polypeptide (PP) has been isolated from pancreatic extracts of the herring gull (Larus argentatus) using a radioimmunoassay employing an antiserum (PP 221), generated to the conserved C-terminal hexapeptide amide of mammalian PP. Gel permeation and reverse-phase HPLC fractionation of crude pancreatic extracts resolved a single molecular form of gull PP in each case. Purification of gull PP to homogeneity indicated that the mammalian PP antiserum employed was only 4% cross-reactive with this peptide. When radioimmunoassay data were corrected for this, the herring gull pancreas was found to contain 5 nmol PP/g wet wt. The molecular mass of gull PP was determined as 4237 +/- 2 Da by 252Cf plasma desorption mass spectroscopy (PDMS). Gas-phase sequencing established unequivocally the entire primary structure of a 36-amino-acid residue peptide as Gly-Pro-Val-Gln-Pro-Thr-Tyr-Pro-Gly-Asp-Asp-Ala-Pro-Val-Glu-Asp-Leu-Val- Arg-Phe - Try-Asn-Asp-Leu-Gln-Gln-Tyr-Leu-Asn-Val-Val-Thr-Arg-His-Arg-Tyr. The computed molecular mass of this peptide (4235.6 Da) was in close agreement with that derived by PDMS. The primary structure of herring gull PP differs from that of the chicken in four residues at positions 3 (Val/Ser), 18 (Val/IIe), 22 (Asn/Asp), and 23 (Asp/Asn). Avian PP thus appears to be a highly conserved regulatory peptide.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Barton CL,Shaw C,Halton DW,Thim Ldoi
10.1006/gcen.1994.1029subject
Has Abstractpub_date
1994-02-01 00:00:00pages
255-9issue
2eissn
0016-6480issn
1095-6840pii
S0016-6480(84)71029-Xjournal_volume
93pub_type
杂志文章abstract：:Urinary corticosterone metabolite responses to capture have recently been shown for the first time in amphibians, and in the present study urinary corticosterone metabolite responses to capture and to confinement in captivity were measured in adult cane toads (Rhinella marina) in Queensland, Australia. An adrenocortic...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.06.015
更新日期：2011-09-01 00:00:00
abstract：:Several antisera specific to neuropeptides of vertebrates were applied to sections of the earthworm Eisenia fetida Sav. Some of them were able to reveal specific neurones of the supraoesophageal ganglion: h-GHRF, CRF, Leu- and Met-enkephalin, dynorphin, substance P, CCK-8, and CCK-8S. A map of different areas or nucle...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90132-8
更新日期：1987-03-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1998.7065
更新日期：1998-05-01 00:00:00
abstract：:The effects of estradiol (E2) on growth hormone (GH) production was investigated in gonad-intact female goldfish. It was first necessary to generate a specific antibody for use in immunocytochemistry, Western, and dot-blot analyses of GH production. To accomplish this, grass carp GH (gcGH) cDNA was cloned by the rever...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1996.6857
更新日期：1997-04-01 00:00:00
abstract：:The pattern and kinetics of degradation of native salmon gonadotropin-releasing hormone (sGnRH) and mammalian leuteinizing hormone-releasing hormone (LHRH) by pituitary bound enzymes were studied in the gilthead seabream, Sparus aurata. sGnRH and LHRH were incubated for different periods of time with membrane or cytos...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(90)90115-3
更新日期：1990-08-01 00:00:00
abstract：:The adenohypophysial cell types in three species of sciaenid teleosts were identified using a combination of classical histochemical techniques and immunocytochemistry with antisera raised against piscine and human pituitary hormones. In general greater specificity for the different cell types was observed with antise...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90086-l
更新日期：1991-12-01 00:00:00
abstract：:We have examined the distribution and physiological effects of three evolutionarily and sequence-related neuropeptides in Rhodnius prolixus. These neuropeptides, adipokinetic hormone (RhoprAKH), corazonin (CRZ) and adipokinetic hormone/corazonin-related peptide (RhoprACP) are present in distinct, non-overlapping neuro...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2013.10.012
更新日期：2014-01-01 00:00:00
abstract：:Testosterone has been suggested to be involved in the regulation of male territorial behavior. For example, seasonal peaks in testosterone typically coincide with periods of intense competition between males for territories and mating partners. However, some species also express territorial behavior outside a breeding...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.11.027
更新日期：2013-04-01 00:00:00
abstract：:This study investigates the effects of exogenous thyroxine (T4) on running endurance, tissue masses, and the activities of citrate synthase (CS), pyruvate kinase (PK), cytosolic alpha-glycerophosphate dehydrogenase (alpha-GPDH), and beta-hydroxyacyl Coenzyme A dehydrogenase (HOAD) in Sceloporus undulatus (eastern fenc...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90132-p
更新日期：1991-01-01 00:00:00
abstract：:Four genomes and two transcriptomes from six Chelicerate species were analyzed for the presence of neuropeptide and neurohormone precursors and their GPCRs. The genome from the spider Stegodyphus mimosarum yielded 87 neuropeptide precursors and 101 neuropeptide GPCRs. High neuropeptide transcripts were also found in t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.07.014
更新日期：2016-11-09 00:00:00
abstract：:In altricial songbirds, food restriction in early development has adverse effects on various physiological systems. When conditions improve birds can accelerate growth, but this compensatory strategy is associated with long-term adverse consequences. One system affected by altered growth rates is the hypothalamic-pitu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.08.001
更新日期：2015-10-01 00:00:00
abstract：:Chicken growth hormone (cGH) has been shown to affect chicken skeletal muscle satellite cell proliferation and differentiation in vitro. This study describes the interactions of cGH with basic fibroblast growth factor (bFGF) and insulin-like growth factor I (IGF-I). Both cGH and bFGF induced cGH receptor (cGH-R) gene ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1997.6964
更新日期：1997-10-01 00:00:00
abstract：:cDNA encoding the beta type of thyroid hormone receptor (THR) was cloned from a lambda gt10 library prepared from the whole bodies of metamorphosing flounder (Paralichthys olivaceus). The deduced amino acid sequence of the flounder THR beta (fTHR beta 1) showed higher homologies to the known THR beta s of other verteb...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1995.1102
更新日期：1995-08-01 00:00:00
abstract：:A reliable and rapid technique for the measurement of estradiol-17 beta, testosterone, progesterone, 17 alpha-hydroxyprogesterone, 17 alpha,20 beta-dihydroxy-4-pregnen-3-one, and cortisol by reverse-phase high-performance liquid chromatography and radioimmunoassay in single female goldfish serum samples of 50 microlit...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(89)90135-4
更新日期：1989-12-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2000.7571
更新日期：2000-12-01 00:00:00
abstract：:In order to examine the role of thyroid hormones during salmonid development, techniques were developed for quantitative extraction of thyroxine from eggs, whole embryos, and alevins of chum salmon (Oncorhynchus keta) at various stages of development. Frozen eggs, embryos, alevins, or fry were homogenized in ice-cold ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90068-2
更新日期：1987-10-01 00:00:00
abstract：:Hormones play key, functional roles in mediating the tradeoff between survival and reproduction. Glucocorticoid hormones can inhibit reproduction and improve chances of survival during periods of stress. However, glucocorticoid hormones are, at times, also associated with successfully engaging in energetically costly ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.08.008
更新日期：2014-11-01 00:00:00
abstract：:Androgens take part in the regulation of puberty and promote growth and development. They play their biological role by binding to a specific androgen receptor (AR). The aim of this study was to evaluate the expression of AR mRNA and protein in the pituitary and adrenal glands, to localize AR protein in luteinizing ho...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.03.001
更新日期：2015-06-01 00:00:00
abstract：:Serum prolactin (PRL) and luteinizing hormone (LH) levels from mature female turkeys were determined following ovariectomy and daily subcutaneous injections for 4 or 9 days of 0.002-2.0 mg/kg estradiol benzoate (EB), 0.05-2.0 mg/kg progesterone (P), or their combinations. Serum PRL levels were increased (P less than 0...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90159-4
更新日期：1983-10-01 00:00:00
abstract：:Ecdysteroids play an essential role in the regulation of the molting processes of arthropods. Nuclear receptors of the spider Agelena silvatica that showed high homology with other arthropods especially in the functional domains were identified, two isoforms of ecdysone receptor (AsEcRA, AsEcRB), retinoid X receptor (...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2017.01.032
更新日期：2017-06-01 00:00:00
abstract：:The steroid hormone 5α-dihydrotestosterone (DHT) is one of the most physiologically important androgens in male vertebrates, with the exception of teleost fish, in which it is generally assumed that DHT does not play any major physiological role. However, this assumption is challenged by the fact that all the componen...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2013.06.017
更新日期：2013-09-15 00:00:00
abstract：:Pinealectomized and intact chicks raised under cyclic (14L:10D) and noncyclic (OL;24L) conditions of environmental lighting were sampled 60 hr and 6 weeks after pinealectomy to evaluate the effects of pineal ablation on plasma and ocular melatonin concentrations. Little or no immunoreactive melatonin was present in pl...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(85)90114-5
更新日期：1985-06-01 00:00:00
abstract：:Variation in food intake affects somatic growth by altering the expression of hormones in the somatotropic endocrine axis including insulin-like growth factor-1 (IGF-1). Here, we examined IGF-1 pathway responses to long- and short-term variation in food availability in copper rockfish (Sebastes caurinus), a nearshore ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2019.04.025
更新日期：2019-09-01 00:00:00
abstract：:The recent purification of two gonadotropins, GTH I and GTH II, in teleost fish and the development of their specific radioimmunoassays using antibodies directed against their beta subunits have demonstrated that earlier assays for GTH II also measured GTH I. Most of the results on the gonadotropic control of reproduc...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1998.7088
更新日期：1998-07-01 00:00:00
abstract：:The oocytes of the freshwater catfish Heteropneustes fossilis hydrate during hormone-induced meiotic maturation. To investigate if this process may be mediated by aquaporins (AQPs), as it occurs in marine fish producing highly hydrated eggs, the cloning of ovarian AQPs in catfish was carried out. Using degenerate prim...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.10.002
更新日期：2011-01-01 00:00:00
abstract：:Somatostatin receptors (sst) mediate the numerous physiological actions (e.g., aspects of growth, development, and metabolism) of the somatostatin family of peptides. In this study, we used rainbow trout (Oncorhynchus mykiss) to establish the pattern of sst subtype 1A, 1B, and 2 mRNA expression in selected tissues (op...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2004.09.008
更新日期：2004-12-01 00:00:00
abstract：:The regulated secretory pathway is a hallmark of endocrine and neuroendocrine cells. This process comprises different sequential steps, including ER-associated protein synthesis, ER-to-Golgi protein transport, Golgi-associated posttranslational modification, sorting and packing of secretory proteins into carrier granu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2011.08.017
更新日期：2012-01-01 00:00:00
abstract：:The penultimate instar larvae of Mamestra brassicae have one ecdysteroid peak 2 days after ecdysis which induces larval ecdysis. Allatectomy deleted this peak and caused a larger peak 7 days later just before precocious pupation, in a pattern similar to the ecdysteroid titer of the last instar. Application of juvenile...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90157-7
更新日期：1986-08-01 00:00:00
abstract：:Food abundance is closely associated with reproductive readiness in vertebrates. Food scarcity can activate the hypothalamo-pituitary-adrenal axis, decrease sex steroid secretion, and dampen reproductive behavior. However, the mechanisms underlying these transient effects are unclear. Gonadotropin inhibitory hormone (...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.03.010
更新日期：2015-05-01 00:00:00
abstract：:Although 11-ketotestosterone (11-KT) has been found in blood of females of several diadromous fish species, the importance, abundance, and prevalence of this and related 11-oxygenated androgens in females have not been investigated. To address this issue and to determine whether the differences among androgen profiles...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(02)00562-2
更新日期：2002-10-15 00:00:00
