当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Crystallization of catechol-1,2 dioxygenase from Pseudomonas arvilla C-1.

Crystallization of catechol-1,2 dioxygenase from Pseudomonas arvilla C-1.

Abstract：

:The metalloenzyme catechol 1,2-dioxygenase from Pseudomonas arvilla C-1 consists of three isozymes formed by combinations of two non-identical subunits; alpha alpha, alpha beta and beta beta; with molecular masses of 59,000, 63,000 and 67,000 Da, respectively. The alpha alpha isozyme crystallizes in the orthorhombic space group C222(1) with unit cell dimensions a = 62.7 A, b = 71.5 A, c = 187.1 A. The rectangular plates diffract to 2.6 A resolution. This is the first dioxygenase to be crystallized that uses catechol as a substrate. Comparison of the structure of this enzyme with protocatechuate 3,4-dioxygenase will provide basic information about the mechanisms of subunit association, substrate selectivity, and the origins of metabolic diversity in enzymes.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Earhart CA,Hall MD,Michaud-Soret I,Que L Jr,Ohlendorf DH

doi

10.1006/jmbi.1994.1144

subject

Has Abstract

pub_date

1994-02-11 00:00:00

pages

377-8

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(84)71144-2

journal_volume

236

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • A symmetrical model for the domain structure of type I DNA methyltransferases.

    abstract：:Type I DNA methyltransferases are complex multisubunit enzymes that methylate a specific base in each half of an asymmetric bipartite DNA recognition sequence. The specificity (S) subunit contains two corresponding DNA sequence recognition domains, plus a number of conserved regions which interact with two modificatio...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1006/jmbi.1994.1624

    authors： Kneale GG

    更新日期：1994-10-14 00:00:00

  • Neuronal BC1 RNA: microtubule-dependent dendritic delivery.

    abstract：:RNA localization is an important means of post-transcriptional regulation of gene expression in many eukaryotic cell types. In neurons, select RNAs are delivered to postsynaptic dendritic microdomains, a mechanism that is considered a key underpinning in the administration of long-term synaptic plasticity. BC1 RNA is ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2005.11.090

    authors： Cristofanilli M,Iacoangeli A,Muslimov IA,Tiedge H

    更新日期：2006-03-10 00:00:00

  • Two-dimensional crystal packing of matrix porin. A channel forming protein in Escherichia coli outer membranes.

    abstract：:Two-dimensional crystalline porin sheets were obtained by reconstitution of monodisperse protein trimers and phospholipids (dimyristoylphosphatidylcholine) by detergent dialysis, analogous to the reconstitution method used for functional tests (Schindler & Rosenbusch, 1981). Three different packing arrangements were o...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(83)80275-7

    authors： Dorset DL,Engel A,Häner M,Massalski A,Rosenbusch JP

    更新日期：1983-04-25 00:00:00

  • Structure and mechanism of the magnesium-independent aromatic prenyltransferase CloQ from the clorobiocin biosynthetic pathway.

    abstract：:CloQ is an aromatic prenyltransferase from the clorobiocin biosynthetic pathway of Streptomyces roseochromogenes var. oscitans. It is involved in the synthesis of the prenylated hydroxybenzoate moiety of the antibiotic, specifically catalyzing the attachment of a dimethylallyl moiety to 4-hydroxyphenylpyruvate. Herein...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.09.067

    authors： Metzger U,Keller S,Stevenson CE,Heide L,Lawson DM

    更新日期：2010-12-10 00:00:00

  • Alternative splicing of an amino-terminal PEVK-like region generates multiple isoforms of Drosophila projectin.

    abstract：:Drosophila projectin is an extremely large protein found within the muscle sarcomeric unit, parallel with the actin and myosin filaments. Projectin has been suggested as the elastic component of C-filaments in insect indirect flight muscles, which is consistent with its localization from the Z band to the tip of the A...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.5115

    authors： Southgate R,Ayme-Southgate A

    更新日期：2001-11-09 00:00:00

  • Structural basis of TBX5-DNA recognition: the T-box domain in its DNA-bound and -unbound form.

    abstract：:TBX5, a member of the T-box transcription factor family, plays an important role in heart and limb development. More than 60 single point or deletion mutations of human TBX5 are associated with Holt-Oram syndrome that manifests itself as heart and limb malformations in 1 out of 100,000 live births. The majority of the...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.04.052

    authors： Stirnimann CU,Ptchelkine D,Grimm C,Müller CW

    更新日期：2010-07-02 00:00:00

  • Site-directed mutagenesis on human cystathionine-gamma-lyase reveals insights into the modulation of H2S production.

    abstract：:In recent years, increased interest has been directed towards hydrogen sulfide (H2S) as the third gasotransmitter and its role in various diseases. Cystathionine-gamma-lyase (CSE) is one of the enzymes responsible for the endogenous production of H2S in mammals. With the aid of the crystal structures of human CSE and ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.11.058

    authors： Huang S,Chua JH,Yew WS,Sivaraman J,Moore PK,Tan CH,Deng LW

    更新日期：2010-02-26 00:00:00

  • Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expression.

    abstract：:The enantioselectivity of lipase from Burkhorderia cepacia KWI-56 has been inverted using a novel in vitro technique for construction and screening of a protein library by single-molecule DNA amplification by PCR followed by in vitro coupled transcription/translation system termed single-molecule-PCR-linked in vitro e...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00782-4

    authors： Koga Y,Kato K,Nakano H,Yamane T

    更新日期：2003-08-15 00:00:00

  • The crystal structure of dTDP-D-Glucose 4,6-dehydratase (RmlB) from Salmonella enterica serovar Typhimurium, the second enzyme in the dTDP-l-rhamnose pathway.

    abstract：:l-Rhamnose is a 6-deoxyhexose that is found in a variety of different glycoconjugates in the cell walls of pathogenic bacteria. The precursor of l-rhamnose is dTDP-l-rhamnose, which is synthesised from glucose- 1-phosphate and deoxythymidine triphosphate (dTTP) via a pathway requiring four enzymes. Significantly this ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4470

    authors： Allard ST,Giraud MF,Whitfield C,Graninger M,Messner P,Naismith JH

    更新日期：2001-03-16 00:00:00

  • Three-dimensional structure and substrate binding of Bacillus stearothermophilus neopullulanase.

    abstract：:Crystal structures of Bacillus stearothermophilus TRS40 neopullulanase and its complexes with panose, maltotetraose and isopanose were determined at resolutions of 1.9, 2.4, 2.8 and 3.2A, respectively. Since the latter two carbohydrates are substrates of this enzyme, a deactivated mutant at the catalytic residue Glu35...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)01402-x

    authors： Hondoh H,Kuriki T,Matsuura Y

    更新日期：2003-02-07 00:00:00

  • A structural perspective of the sequence variability within botulinum neurotoxin subtypes A1-A4.

    abstract：:Botulinum neurotoxin (BoNT) is a category A toxin that has been classified within seven serotypes, designated A-G. Recently, it has been discovered that sequence variability occurs in BoNTs produced by serotype A (BoNT/A) variant strains, designated as subtypes A1 and A2, which have significantly different antibody-bi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.07.040

    authors： Arndt JW,Jacobson MJ,Abola EE,Forsyth CM,Tepp WH,Marks JD,Johnson EA,Stevens RC

    更新日期：2006-09-29 00:00:00

  • The presequence of fumarase is exposed to the cytosol during import into mitochondria.

    abstract：:The majority of mitochondrial proteins can be imported into mitochondria following termination of their translation in the cytosol. Import of fumarase and several other proteins into mitochondria does not appear to occur post-translationally according to standard in vivo and in vitro assays. However, the nature of int...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.02.023

    authors： Karniely S,Regev-Rudzki N,Pines O

    更新日期：2006-04-28 00:00:00

  • Interior and surface of monomeric proteins.

    abstract：:The solvent-accessible surface area (As) of 46 monomeric proteins is calculated using atomic co-ordinates from high-resolution and well-refined crystal structures. The As of these proteins can be determined to within 1 to 2% and that of their individual residues to within 10 to 20%. The As values of proteins are corre...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(87)90038-6

    authors： Miller S,Janin J,Lesk AM,Chothia C

    更新日期：1987-08-05 00:00:00

  • Dimer form of phosphorylated Spo0A, a transcriptional regulator, stimulates the spo0F transcription at the initiation of sporulation in Bacillus subtilis.

    abstract：:The Spo0A protein of Bacillus subtilis is a transcriptional regulator that shows extensive homology to the regulator proteins in bacterial two-component regulatory systems. Phosphorylation of Spo0A is absolutely necessary for the initiation of sporulation. We now show that phospho-Spo0A is a dimer, binds specifically ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0354

    authors： Asayama M,Yamamoto A,Kobayashi Y

    更新日期：1995-06-30 00:00:00

  • Ion-coupling determinants of Na+-driven and H+-driven flagellar motors.

    abstract：:The bacterial flagellar motor is a tiny molecular machine that uses a transmembrane flux of H(+) or Na(+) ions to drive flagellar rotation. In proton-driven motors, the membrane proteins MotA and MotB interact via their transmembrane regions to form a proton channel. The sodium-driven motors that power the polar flage...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00096-2

    authors： Asai Y,Yakushi T,Kawagishi I,Homma M

    更新日期：2003-03-21 00:00:00

  • Crystal structure of SpoVT, the final modulator of gene expression during spore development in Bacillus subtilis.

    abstract：:Endospore formation in Bacillus subtilis is orchestrated by five developmental sigma factors and further modulated by several auxiliary transcription factors. One of these, SpoVT, regulates forespore-specific sigma(G)-dependent genes and plays a key role in the final stages of spore formation. We have determined the c...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2008.10.061

    authors： Asen I,Djuranovic S,Lupas AN,Zeth K

    更新日期：2009-03-06 00:00:00

  • The molecular basis of vitamin E retention: structure of human alpha-tocopherol transfer protein.

    abstract：:Alpha-tocopherol transfer protein (alpha-TTP) is a liver protein responsible for the selective retention of alpha-tocopherol from dietary vitamin E, which is a mixture of alpha, beta, gamma, and delta-tocopherols and the corresponding tocotrienols. The alpha-TTP-mediated transfer of alpha-tocopherol into nascent VLDL ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00724-1

    authors： Meier R,Tomizaki T,Schulze-Briese C,Baumann U,Stocker A

    更新日期：2003-08-15 00:00:00

  • Crystallization and preliminary X-ray analysis of Gc, the vitamin D-binding protein in serum.

    abstract：:The vitamin D-binding protein, Gc, was purified from human serum and crystallized using the hanging-drop method. The best crystals were grown from 28% polyethylene glycol 400 in 50 mM-sodium acetate at pH 4.8. These crystals diffract to 3.4 A and the observed diffraction is consistent with orthorhombic space groups P4...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(91)90097-p

    authors： Vogelaar NJ,Lindberg U,Schutt CE

    更新日期：1991-08-05 00:00:00

  • Translation driven by picornavirus IRES is hampered from Sindbis virus replicons: rescue by poliovirus 2A protease.

    abstract：:Alphavirus replicons are very useful for analyzing different aspects of viral molecular biology. They are also useful tools in the development of new vaccines and highly efficient expression of heterologous genes. We have investigated the translatability of Sindbis virus (SV) subgenomic mRNA bearing different 5'-untra...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.07.014

    authors： Sanz MA,Welnowska E,Redondo N,Carrasco L

    更新日期：2010-09-10 00:00:00

  • Determining the Limitations and Benefits of Noise in Gene Regulation and Signal Transduction through Single Cell, Microscopy-Based Analysis.

    abstract：:Stochastic fluctuations, termed "noise," in the level of biological molecules can greatly impact cellular functions. While biological noise can sometimes be detrimental, recent studies have provided an increasing number of examples in which biological noise can be functionally beneficial. Rather than provide an exhaus...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2017.03.007

    authors： Harton MD,Batchelor E

    更新日期：2017-04-21 00:00:00

  • A novel allosteric mechanism in haemoglobin. Structure of bovine deoxyhaemoglobin, absence of specific chloride-binding sites and origin of the chloride-linked Bohr effect in bovine and human haemoglobin.

    abstract：:The structure of bovine deoxyhaemoglobin has been determined at 2.2 A resolution and refined to an R-factor of 0.193 for all 32,583 reflections, and a free R-factor of 0.249 for 1527 reflections excluded from the refinement. The structure shows no significant differences between the alpha-carbon positions of bovine an...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1993.1530

    authors： Perutz MF,Fermi G,Poyart C,Pagnier J,Kister J

    更新日期：1993-10-05 00:00:00

  • The DisVis and PowerFit Web Servers: Explorative and Integrative Modeling of Biomolecular Complexes.

    abstract：:Structure determination of complex molecular machines requires a combination of an increasing number of experimental methods with highly specialized software geared toward each data source to properly handle the gathered data. Recently, we introduced the two software packages PowerFit and DisVis. These combine high-re...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2016.11.032

    authors： van Zundert GC,Trellet M,Schaarschmidt J,Kurkcuoglu Z,David M,Verlato M,Rosato A,Bonvin AM

    更新日期：2017-02-03 00:00:00

  • The critical roles of residues P235 and F236 of subunit A of the motor protein A-ATP synthase in P-loop formation and nucleotide binding.

    abstract：:The mutants P235A and F236A have been generated and their crystal structure was determined to resolutions of 2.38 and 2.35 A, respectively, in order to understand the residues involved in the formation of the novel arched P-loop of subunit A of the A-ATP synthase from Pyrococcus horikoshii OT3. Both the structures sho...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.06.070

    authors： Kumar A,Manimekalai MS,Balakrishna AM,Priya R,Biuković G,Jeyakanthan J,Grüber G

    更新日期：2010-09-03 00:00:00

  • Dissecting specificity in the Janus kinases: the structures of JAK-specific inhibitors complexed to the JAK1 and JAK2 protein tyrosine kinase domains.

    abstract：:The Janus kinases (JAKs) are a pivotal family of protein tyrosine kinases (PTKs) that play prominent roles in numerous cytokine signaling pathways, with aberrant JAK activity associated with a variety of hematopoietic malignancies, cardiovascular diseases and immune-related disorders. Whereas the structures of the JAK...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.01.041

    authors： Williams NK,Bamert RS,Patel O,Wang C,Walden PM,Wilks AF,Fantino E,Rossjohn J,Lucet IS

    更新日期：2009-03-20 00:00:00

  • Titration calculations of foot-and-mouth disease virus capsids and their stabilities as a function of pH.

    abstract：:Foot-and-mouth disease virus (FMDV), a non-enveloped picornavirus, is sensitive to acidic conditions. At pH values below 7 the icosahedral virus capsid, formed from 60 copies of a protomer containing four polypeptides (VP1 to 4), dissociates into 12 pentamers, releasing the viral RNA. Evidence suggests that this acid ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1418

    authors： van Vlijmen HW,Curry S,Schaefer M,Karplus M

    更新日期：1998-01-16 00:00:00

  • On the connection between inherent DNA flexure and preferred binding of hydroxymethyluracil-containing DNA by the type II DNA-binding protein TF1.

    abstract：:TF1 is a member of the family of type II DNA-binding proteins, which also includes the bacterial HU proteins and the Escherichia coli integration host factor (IHF). Distinctive to TF1, which is encoded by the Bacillus subtilis bacteriophage SPO1, is its preferential binding to DNA in which thymine is replaced by 5-hyd...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0392

    authors： Grove A,Galeone A,Mayol L,Geiduschek EP

    更新日期：1996-07-12 00:00:00

  • Topological frustration in beta alpha-repeat proteins: sequence diversity modulates the conserved folding mechanisms of alpha/beta/alpha sandwich proteins.

    abstract：:The thermodynamic hypothesis of Anfinsen postulates that structures and stabilities of globular proteins are determined by their amino acid sequences. Chain topology, however, is known to influence the folding reaction, in that motifs with a preponderance of local interactions typically fold more rapidly than those wi...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.03.001

    authors： Hills RD Jr,Kathuria SV,Wallace LA,Day IJ,Brooks CL 3rd,Matthews CR

    更新日期：2010-04-30 00:00:00

  • Prediction of the activity and stability effects of site-directed mutagenesis on a protein core.

    abstract：:When theoretical methods are used to predict the properties of a given system, such as the effects of the substitution of a specific amino acid on the activity or stability of a protein as a whole, the accuracy of the prediction is directly dependent on the validity of the underlying model. A common error, however, is...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(92)90895-q

    authors： van Gunsteren WF,Mark AE

    更新日期：1992-09-20 00:00:00

  • A comparison of the pH, urea, and temperature-denatured states of barnase by heteronuclear NMR: implications for the initiation of protein folding.

    abstract：:The denatured states of barnase that are induced by urea, acid, and high temperature and acid have been assigned and characterised by high resolution heteronuclear NMR. The assignment was completed using a combination of triple-resonance and magnetisation-transfer methods. The latter was facilitated by selecting a sui...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0618

    authors： Arcus VL,Vuilleumier S,Freund SM,Bycroft M,Fersht AR

    更新日期：1995-11-24 00:00:00

  • Mitochondrial respiratory chain supercomplexes are destabilized in Barth Syndrome patients.

    abstract：:Mutations in the human TAZ gene are associated with Barth Syndrome, an often fatal X-linked disorder that presents with cardiomyopathy and neutropenia. The TAZ gene encodes Tafazzin, a putative phospholipid acyltranferase that is involved in the remodeling of cardiolipin, a phospholipid unique to the inner mitochondri...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2006.06.057

    authors： McKenzie M,Lazarou M,Thorburn DR,Ryan MT

    更新日期：2006-08-18 00:00:00