Abstract:
AIMS:To compare prognostic information obtained by image analysis cytometry of paraffin wax embedded soft tissue sarcomas with conventional assessment. METHODS:A CAS 200 image analyser was used to determine DNA content of Feulgen stained cytology preparations and tissue sections and to quantify immunostaining by Ki67 and PC10 antibodies. A mitotic count in 50 high power fields was undertaken and histological grade assigned by the Trojani system. Clinical details including follow up and outcome were obtained by case note review. The Kruskal-Wallis one way analysis test, Spearman rho significance test, Kaplan-Meier method, and log-rank test were applied in statistical analysis. RESULTS:Ploidy status, DNA index, 2.5c exceeding rate, 5c exceeding rate, mitotic count and Trojani grade all correlated significantly with clinical outcome. The relation between Ki67 index and outcome did not reach significance. The PC10 index and outcome were not related. Only 2.5c exceeding rate, 5c exceeding rate, and mitotic count correlated significantly with Trojani grade. CONCLUSIONS:DNA content determination of soft tissue sarcomas by image analysis provides quantifiable information of benefit in prediction of outcome. Larger series are required to determine the independent value of ploidy. In this study quantification of anti-Ki67 and anti-PC10 immunostaining was not of prognostic benefit) by contrast with mitotic count and Trojani grade.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Michie BA,Black C,Reid RP,Barrett A,Hamblen DLdoi
10.1136/jcp.47.5.443subject
Has Abstractpub_date
1994-05-01 00:00:00pages
443-7issue
5eissn
0021-9746issn
1472-4146journal_volume
47pub_type
杂志文章abstract::Staphylococcus aureus isolated between 1967 and 1975 from the nose and skin lesions of patients in dermatology wards and from the burns of patients in a burns unit in Birmingham showed a high incidence of multiple antibiotic-resistant strains in both environments. Over 20% of the strains isolated from patients on admi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.1.40
更新日期:1977-01-01 00:00:00
abstract::Sera obtained from 34 men with Chlamydia trachomatis positive non-gonococcal urethritis, 34 men with C trachomatis negative non-gonococcal urethritis, 42 women with acute salpingitis, 38 healthy women, and 34 healthy men were studied for the presence of specific serum C trachomatis IgA and IgG antibodies. Serological ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.6.686
更新日期:1984-06-01 00:00:00
abstract:AIM:Primary biliary cirrhosis (PBC) is characterised by antimitochondrial antibody against the pyruvate dehydrogenase complex (PDC) and chronic non-suppurative destructive cholangitis (CNSDC). Pyruvate oxidation to acetyl-CoA by PDC is a key step in the glycolytic system. Oestrogen-related receptor-α (ERRα) is function...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2013-201815
更新日期:2014-05-01 00:00:00
abstract:INTRODUCTION:Whole slide images (WSI) have been used in many pathology applications such as teleconsultation, teaching and research, but not in primary diagnostics. The aim of this study was to test the feasibility of using WSI in primary diagnostics of paediatric pathology specimens and placental tissue. MATERIALS AN...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2012-201104
更新日期:2013-03-01 00:00:00
abstract::The development and evaluation of a new selective medium for Streptococcus pneumoniae is described. It is shown that the new medium (crystal violet-nalidixic acid-gentamicin agar:CVNG) is highly selective for Strep. pneumoniae in sputum specimens containing other organisms and is also sensitive, being only marginally ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.33.8.770
更新日期:1980-08-01 00:00:00
abstract::A patient with hyperparathyroidism due to a parathyroid adenoma was found to have metastatic medullary carcinoma in lymph nodes close to the thyroid gland. The finding of normal calcitonin and parathormone concentrations after removal of the adenoma suggested that the parathyroid lesion was not the result of the hypoc...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.24.9.851
更新日期:1971-12-01 00:00:00
abstract::The relation between the choline containing surfactant phospholipids lecithin and sphingomyelin in amniotic fluid and fetal lung maturity is well established. An enzymatic method that had been automated and optimised for use on a centrifugal analyser was used to measure the total choline containing phospholipids in am...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.11.1304
更新日期:1985-11-01 00:00:00
abstract::Serious infection caused by Lancefield group C streptococci is unusual in man. Two unrelated deaths associated with these organisms in a 55 year old woman who died after three days of diarrhoea and vomiting, and in a 65 old man who died after a week of non-specific symptoms, are presented. Post mortem examination show...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.10.965
更新日期:1993-10-01 00:00:00
abstract::The sellar region is the site of a large number of pathological entities arising from the pituitary and adjacent anatomical structures, including brain, blood vessels, nerves and meninges. The surgical pathology of this area requires the accurate identification of neoplastic lesions, including pituitary adenoma and ca...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2005.031187
更新日期:2006-12-01 00:00:00
abstract::Tubulocystic carcinoma (TCC) is a very rare renal tumour with unique gross and microscopic features, alternatively considered as low-grade collecting duct carcinoma. Recent studies favoured distinction of TCC from collecting duct carcinoma, and some cases of TCC synchronously coexisting with other renal cell tumour su...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202363
更新日期:2014-11-01 00:00:00
abstract::Temporal changes in gastric antibody response were investigated in 113 (51 men, 62 women) patients with confirmed pernicious anaemia. Their ages ranged from 31-92 years (mean (SD 13.2) 66). At diagnosis, parietal cell antibody and intrinsic factor antibody were detected in 90.9% and 39.1% of all patients, respectively...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.10.1092
更新日期:1989-10-01 00:00:00
abstract:AIMS:To investigate the serological cross reaction between legionella and campylobacter using the rapid microagglutination test (RMAT). METHODS:Serum samples from 49 patients with campylobacter infection were tested for legionella antibodies using the indirect fluorescent antibody test (IFAT) and the RMAT. Serum sampl...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.49.7.584
更新日期:1996-07-01 00:00:00
abstract::Xanthine oxidase (XO) activity was found to be negligible in sterile human urines (less than 480 units, as presently defined, per litre). Significant XO activity was found in all urines containing more than 10(5) bacteria/ml, except for urines infected with Staphylococcus aureus, in which XO activity ranged from 347 t...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.31.5.444
更新日期:1978-05-01 00:00:00
abstract::Three cases of plasmacytoma of the thyroid are described and their clinical and histological features recorded. Two were considered to be true extramedullary plasmacytomas, the third the initial manifestation of a disseminated myelomatous process. Plasmacytomas of the thyroid are shown to have many features in common ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.21.5.661
更新日期:1968-09-01 00:00:00
abstract:AIMS:PCR amplicon-based next-generation sequencing (NGS) panels are increasingly used for clinical diagnostic assays. Amplification bias is a well-known limitation of PCR amplicon-based approaches. We sought to characterise lower-performance amplicons in an off-the-shelf NGS panel (TruSight Myeloid Sequencing Panel) fo...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2015-203580
更新日期:2016-09-01 00:00:00
abstract::Review of published work and analysis of clinical data and pathology of four biopsy specimens from two patients with laryngeal contact granuloma showed that its peptic origin was derived from a gastro-oesophago-laryngeal reflux. It is proposed that the term "peptic granuloma" should be given to this phenomenon. This t...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.8.800
更新日期:1989-08-01 00:00:00
abstract:AIMS:Cervical cytology biobanking is a feasible concept in cervical pathology and could be an indispensable tool for fundamental and applied molecular biological research. PCR is a powerful molecular technique that can be performed on a variety of cervical sample types including Pap-stained cervical smears. However, si...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2007.052688
更新日期:2008-05-01 00:00:00
abstract::A slight modification in tissue processing and staining technique enables a previously described method for staining aluminium to be used to demonstrate aluminium in osteomalacia associated with haemodialysis. The stain appears to be accurate in diagnosing this condition and may assist in establishing the diagnosis be...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.34.12.1352
更新日期:1981-12-01 00:00:00
abstract:AIM:The immunoproteasome is a specific proteasome isoform whose proteolytic activity enhances the generation of antigenic peptides to be presented by major histocompatibility complex class I molecules to CD8+ T cells. Physiologically, it is expressed abundantly in immune cells and is induced in somatic cells by cytokin...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2020-206618
更新日期:2020-09-17 00:00:00
abstract:AIMS:To describe characteristics of a series of people accidentally and deliberately killed by air powered weapons. METHODS:Five cases of fatal airgun injury were identified by forensic pathologists and histopathologists. The circumstances surrounding the case, radiological examination, and pathological findings are d...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.7.525
更新日期:1998-07-01 00:00:00
abstract:BACKGROUND:RCAS1 (receptor binding cancer antigen expressed on SiSo cells) is a tumour associated antigen. It is involved in immune evasion by tumour cells, by binding to receptors on cells involved in the immune response, such as T cells and natural killer cells, and inducing apoptosis. High expression of RCAS1 has be...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.56.10.764
更新日期:2003-10-01 00:00:00
abstract::An enzyme linked immunosorbent assay (ELISA) to measure free serum IgG antibodies to Pseudomonas aeruginosa in patients with cystic fibrosis was developed. Seven strains of P aeruginosa cells, treated with glutaraldehyde and representing the most commonly isolated serotypes in our cystic fibrosis unit, were used. The ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.10.1124
更新日期:1986-10-01 00:00:00
abstract::It is found that serum antifibrinolytic activity is inhibitory both to the spontaneous fibrinolytic activity and to the activity of streptokinase-activated plasminogen. The titres of serum antiplasmin activity during gestation, labour, and the puerperium are all of the same order and do not differ from those in non-pr...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.13.3.220
更新日期:1960-05-01 00:00:00
abstract::This report describes a case of mucinous carcinoma and Müllerian metaplasia arising within an appendiceal duplication cyst found incidentally during an emergency Caesarian section. Intestinal duplication cysts are rare and although there are occasional reports of malignant transformation, this is the first case where ...
journal_title:Journal of clinical pathology
pub_type: 信件
doi:10.1136/jcp.55.7.551
更新日期:2002-07-01 00:00:00
abstract::Conventional prostate adenocarcinomas consist mainly of tumour cells of luminal immunophenotype with scattered neuroendocrine (NE) cells. NE cells are defined by chromogranin A (CGA) immunoreactivity. Unlike luminal cells, NE cells lack androgen receptor (AR) and prostate specific antigen (PSA) immunoreactivity. This ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2003.010207
更新日期:2004-03-01 00:00:00
abstract:AIM:To investigate the association between the quantity of gastric chemokine mRNA expression, severity of gastritis, and cagA positivity in Helicobacter pylori associated gastritis. METHODS:In 83 dyspeptic patients, antral and corpus biopsies were taken for semiquantitative reverse transcription polymerase chain react...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.10.765
更新日期:1998-10-01 00:00:00
abstract::A patient is described in whom a campylobacter enteritis closely resembled ulcerative colitis on clinical, sigmoidoscopic, and histological grounds. Selective stool culture techniques may be necessary to differentiate campylobacter colitis from ulcerative colitis proper. ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.32.10.986
更新日期:1979-10-01 00:00:00
abstract:AIM:To evaluate the use of multimedia enhancements, using a computerised microscope, in the training of microscope skills. METHODS:The HOME microscope provides facilities to highlight features of interest in conjunction with either text display or aural presentation. A pilot study was carried out with 10 individuals, ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.51.4.330
更新日期:1998-04-01 00:00:00
abstract:AIMS:The generation and characterisation of a monoclonal antibody that specifically recognises the mdr-1 encoded protein, P-glycoprotein (P-170), on routinely processed formalin fixed, paraffin wax embedded tissue sections. METHODS:The monoclonal antibody, designated 6/1C, was produced following a combination of in vi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.50.6.465
更新日期:1997-06-01 00:00:00
abstract::Expression profiling has been extensively applied to the study of breast cancer and undoubtedly is changing the way breast cancer is perceived. Over the past few years, several groups have described prognostic "signatures" (gene lists) that are purported to be more accurate prognostic factors than well established cli...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2005.028324
更新日期:2006-03-01 00:00:00