Functional neuroanatomy of antisaccade eye movements investigated with positron emission tomography.

abstract：:Explanted mouse blastocysts were microinjected in the blastocoel cavity with simian virus 40 (SV40) viral DNA. After surgical transfer to the uteri of pseudopregnant surrogate mothers, approximately 40% of the blastocysts developed to term and became healthy adults without apparent tumors at 1 year of age. Molecular h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jaenisch R,Mintz B

更新日期：1974-04-01 00:00:00

abstract：:Gene expression networks are complicated by the assortment of regulatory factors that bind DNA and modulate transcription combinatorially. Single-cell measurements can reveal biological mechanisms hidden by population averages, but their value has not been fully explored in the context of mRNA regulation. Here, we ada...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang L,Brugge JS,Janes KA

更新日期：2011-10-04 00:00:00

abstract：:For many intraoperative decisions surgeons depend on frozen section pathology, a technique developed over 150 y ago. Technical innovations that permit rapid molecular characterization of tissue samples at the time of surgery are needed. Here, using desorption electrospray ionization (DESI) MS, we rapidly detect the tu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Santagata S,Eberlin LS,Norton I,Calligaris D,Feldman DR,Ide JL,Liu X,Wiley JS,Vestal ML,Ramkissoon SH,Orringer DA,Gill KK,Dunn IF,Dias-Santagata D,Ligon KL,Jolesz FA,Golby AJ,Cooks RG,Agar NY

更新日期：2014-07-29 00:00:00

abstract：:Precise genome editing is a valuable tool to study gene function in model organisms. Prime editing, a precise editing system developed in mammalian cells, does not require double-strand breaks or donor DNA and has low off-target effects. Here, we applied prime editing for the model organism Drosophila melanogaster and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bosch JA,Birchak G,Perrimon N

更新日期：2021-01-05 00:00:00

abstract：:Despite extensive studies, the structural basis for the mechanochemical coupling in the rotary molecular motor F1-ATPase (F1) is still incomplete. We performed single-molecule FRET measurements to monitor conformational changes in the stator ring-α3β3, while simultaneously monitoring rotations of the central shaft-γ. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sugawa M,Okazaki K,Kobayashi M,Matsui T,Hummer G,Masaike T,Nishizaka T

更新日期：2016-05-24 00:00:00

abstract：:Micrometeorites with diameter approximately 100-200 microm dominate the flux of extraterrestrial matter on Earth. The vast majority of micrometeorites are chemically, mineralogically, and isotopically related to carbonaceous chondrites, which amount to only 2.5% of meteorite falls. Here, we report the discovery of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gounelle M,Chaussidon M,Morbidelli A,Barrat JA,Engrand C,Zolensky ME,McKeegan KD

更新日期：2009-04-28 00:00:00

abstract：:Coherent oscillations in the theta-to-gamma frequency range have been proposed as a mechanism that coordinates neural activity in large-scale cortical networks in sensory, motor, and cognitive tasks. Whether this mechanism also involves coherent oscillations at delta frequencies (1-4 Hz) is not known. Rather, delta os...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nácher V,Ledberg A,Deco G,Romo R

更新日期：2013-09-10 00:00:00

abstract：:We test the conjecture that becoming unemployed erodes the extent to which a person acknowledges earned entitlement. We use behavioral experiments to generate incentive-compatible measures of individuals' tendencies to acknowledge earned entitlement and incorporate these experiments in a two-stage study. In the first ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barr A,Miller L,Ubeda P

更新日期：2016-04-26 00:00:00

abstract：:Earth's water cycle enables the incorporation of water (hydration) in mantle minerals that can influence the physical properties of the mantle. Lattice thermal conductivity of mantle minerals is critical for controlling the temperature profile and dynamics of the mantle and subducting slabs. However, the effect of hyd...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang YY,Hsieh WP,Tan E,Chen J

更新日期：2017-04-18 00:00:00

abstract：:Replacing Leu-182 by Ala in yeast alcohol dehydrogenase (YADH; alcohol:NAD+ oxidoreductase, EC 1.1.1.1) yields a mutant that retains 34% of its kcat value and makes one stereochemical "mistake" every 850,000 turnovers (instead of approximately 1 error every 7,000,000,000 turnovers in native YADH) in its selection of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weinhold EG,Glasfeld A,Ellington AD,Benner SA

更新日期：1991-10-01 00:00:00

abstract：:Mutations in a number of stress granule-associated proteins have been linked to various neurodegenerative diseases. Several of these mutations are found in aggregation-prone prion-like domains (PrLDs) within these proteins. In this work, we examine the sequence features governing PrLD localization to stress granules u...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boncella AE,Shattuck JE,Cascarina SM,Paul KR,Baer MH,Fomicheva A,Lamb AK,Ross ED

更新日期：2020-03-17 00:00:00

abstract：:A way to assess specific CD8+ T-cell activity in a skin test analogous to the conventional delayed-type hypersensitivity (DTH) reaction for CD4+ T cells is presented. Local injection of viral class I binding peptides caused a specific CD8+ T-cell-mediated DTH in footpads of virally infected mice. The DTH was inducible...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kündig TM,Althage A,Hengartner H,Zinkernagel RM

更新日期：1992-08-15 00:00:00

abstract：:Temperate-zone species have responded to warming temperatures by shifting their distributions poleward and upslope. Thermal tolerance data suggests that tropical species may respond to warming temperatures even more strongly than temperate-zone species, but this prediction has yet to be tested. We addressed this data ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Freeman BG,Class Freeman AM

更新日期：2014-03-25 00:00:00

abstract：:Using defined oligomers of IgE, our group previously studied the quantitative relationship between the aggregation of the high affinity receptors for IgE (Fc epsilonRI) and the earliest signals initiated by such aggregation: the phosphorylation of tyrosines on the receptor. Notably, at certain doses of the oligomers s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Torigoe C,Goldstein B,Wofsy C,Metzger H

更新日期：1997-02-18 00:00:00

abstract：:IL-33, a cytokine of the IL-1 family, is closely associated with type II T cell responses. Here, we report an unexpected proinflammatory role of IL-33 in inflammatory arthritis. IL-33 was expressed in synovial fibroblasts from patients with rheumatoid arthritis (RA). Expression was markedly elevated in vitro by inflam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu D,Jiang HR,Kewin P,Li Y,Mu R,Fraser AR,Pitman N,Kurowska-Stolarska M,McKenzie AN,McInnes IB,Liew FY

更新日期：2008-08-05 00:00:00

abstract：:Two human 12-lipoxygenase enzyme (arachidonate:oxygen 12-oxidoreductase, EC 1.13.11.31)-related genes were characterized from 13 distinct clones isolated from three genomic bacteriophage and cosmid libraries. A complete gene (12-lipoxygenase gene 1) spanning approximately 17 kilobases and consisting of 14 exons with s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Funk CD,Funk LB,FitzGerald GA,Samuelsson B

更新日期：1992-05-01 00:00:00

abstract：:We present a model describing how Mg(2+) binds and stabilizes specific RNA structures. In this model, RNA stabilization arises from two energetically distinct modes of Mg(2+) binding: diffuse- and site-binding. Diffusely bound Mg(2+) are electrostatically attracted to the strong anionic field around the RNA and are ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Misra VK,Draper DE

更新日期：2001-10-23 00:00:00

abstract：:DPB11, a gene that suppresses mutations in two essential subunits of Saccharomyces cerevisiae DNA polymerase II(epsilon) encoded by POL2 and DPB2, was isolated on a multicopy plasmid. The nucleotide sequence of the DPB11 gene revealed an open reading frame predicting an 87-kDa protein. This protein is homologous to th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Araki H,Leem SH,Phongdara A,Sugino A

更新日期：1995-12-05 00:00:00

abstract：:The apoprotein corresponding to the mammalian photoreceptor rhodopsin has been expressed by using suspension cultures of HEK293S cells in defined media that contained 6-15N-lysine and 2-13C-glycine. Typical yields were 1.5-1.8 mg/liter. Incorporation of 6-15N-lysine was quantitative, whereas that of 2-13C-glycine was ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eilers M,Reeves PJ,Ying W,Khorana HG,Smith SO

更新日期：1999-01-19 00:00:00

abstract：:Strongylocentrotus intermedius (A. Agassiz, 1863) is an economically important sea urchin inhabiting the northwest Pacific region of Asia. The northern Primorye (Sea of Japan) populations of S. intermedius consist of two sympatric morphological forms, "usual" (U) and "gray" (G). The two forms are significantly differe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Balakirev ES,Pavlyuchkov VA,Ayala FJ

更新日期：2008-10-21 00:00:00

abstract：:The interaction of interleukin 8 (IL-8) with heparin was studied by using synthetic IL-8 analogs with C- and N-terminal truncations. Elimination of the N-terminal region preceding the first cysteine, which constitutes the IL-8 receptor binding site, did not affect the affinity to heparin-Sepharose. Affinity, however, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Webb LM,Ehrengruber MU,Clark-Lewis I,Baggiolini M,Rot A

更新日期：1993-08-01 00:00:00

abstract：:PAX5 is a well-known haploinsufficient tumor suppressor gene in human B-cell precursor acute lymphoblastic leukemia (B-ALL) and is involved in various chromosomal translocations that fuse a part of PAX5 with other partners. However, the role of PAX5 fusion proteins in B-ALL initiation and transformation is ill-known. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jamrog L,Chemin G,Fregona V,Coster L,Pasquet M,Oudinet C,Rouquié N,Prade N,Lagarde S,Cresson C,Hébrard S,Nguyen Huu NS,Bousquet M,Quelen C,Brousset P,Mancini SJC,Delabesse E,Khamlichi AA,Gerby B,Broccardo C

更新日期：2018-10-09 00:00:00

abstract：:The circadian rhythms of melatonin and body temperature are set to an earlier hour in women than in men, even when the women and men maintain nearly identical and consistent bedtimes and wake times. Moreover, women tend to wake up earlier than men and exhibit a greater preference for morning activities than men. Altho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duffy JF,Cain SW,Chang AM,Phillips AJ,Münch MY,Gronfier C,Wyatt JK,Dijk DJ,Wright KP Jr,Czeisler CA

更新日期：2011-09-13 00:00:00

abstract：:Chlorpromazine (3 x 10(-4)M) prevents the stimulation of adenyl cyclase activity in thyroid membranes produced by thyrotropin and prostaglandin, ACTH stimulation of adenyl cyclase in adrenal tissue, and glucagon- and epinephrine-stimulation of adenyl cyclase activity in liver. Baseline activity is unaffected. Parathyr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolff J,Jones AB

更新日期：1970-02-01 00:00:00

abstract：:We have devised a simple and efficient cDNA cloning strategy that overcomes many of the difficulties encountered in obtaining full-length cDNA clones of low-abundance mRNAs. In essence, cDNAs are generated by using the DNA polymerase chain reaction technique to amplify copies of the region between a single point in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frohman MA,Dush MK,Martin GR

更新日期：1988-12-01 00:00:00

abstract：:The nonneural cholinergic system of immune cells is pivotal for the maintenance of immunological homeostasis. Here we demonstrate the expression of choline acetyltransferase (ChAT) and cholinergic enzymes in murine natural killer (NK) cells. The capacity for acetylcholine synthesis by NK cells increased markedly under...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jiang W,Li D,Han R,Zhang C,Jin WN,Wood K,Liu Q,Shi FD,Hao J

更新日期：2017-07-25 00:00:00

abstract：:Regulation of ion balance in spermatozoa has been shown to be essential for sperm motility and fertility. Control of intracellular ion levels requires the function of distinct ion-transport mechanisms at the cell plasma membrane. Active Na(+) and K(+) exchange in sperm is under the control of the Na,K-ATPase. Two mole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jimenez T,McDermott JP,Sánchez G,Blanco G

更新日期：2011-01-11 00:00:00

abstract：:Adeno-associated virus (AAV) vector delivery of an Apaf-1-dominant negative inhibitor was tested for its antiapoptotic effect on degenerating nigrostriatal neurons in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of Parkinson's disease. The wild-type caspase recruitment domain of Apaf-1 was used as a dom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mochizuki H,Hayakawa H,Migita M,Shibata M,Tanaka R,Suzuki A,Shimo-Nakanishi Y,Urabe T,Yamada M,Tamayose K,Shimada T,Miura M,Mizuno Y

更新日期：2001-09-11 00:00:00

abstract：:Ubiquitin C-terminal hydrolase L1 (UCH-L1) is one of the most abundant and enigmatic enzymes of the CNS. Based on existing UCH-L1 knockout models, UCH-L1 is thought to be required for the maintenance of axonal integrity, but not for neuronal development despite its high expression in neurons. Several lines of evidence...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reinicke AT,Laban K,Sachs M,Kraus V,Walden M,Damme M,Sachs W,Reichelt J,Schweizer M,Janiesch PC,Duncan KE,Saftig P,Rinschen MM,Morellini F,Meyer-Schwesinger C

更新日期：2019-04-16 00:00:00

abstract：:The Pleistocene global dispersal of modern humans required the transit of arid and semiarid regions where the distribution of potable water provided a primary constraint on dispersal pathways. Here, we provide a spatially explicit continental-scale assessment of the opportunities for Pleistocene human occupation of Au...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bird MI,O'Grady D,Ulm S

更新日期：2016-10-11 00:00:00