Consecutive low-usage leucine codons block translation only when near the 5' end of a message in Escherichia coli.

abstract：:The bacterial flagellar motor is a tiny molecular machine that uses a transmembrane flux of H(+) or Na(+) ions to drive flagellar rotation. In proton-driven motors, the membrane proteins MotA and MotB interact via their transmembrane regions to form a proton channel. The sodium-driven motors that power the polar flage...

journal_title：Journal of molecular biology

authors： Asai Y,Yakushi T,Kawagishi I,Homma M

更新日期：2003-03-21 00:00:00

abstract：:The IS1 element contains two adjacent genes called insA and insB, both required for IS1 transposition and IS1-mediated plasmid cointegration. These two genes are transcribed polycistronically from the promoter in the left terminal inverted repeat of IS1 (insL). We constructed overexpression systems of these genes with...

journal_title：Journal of molecular biology

authors： Machida C,Machida Y

更新日期：1989-08-20 00:00:00

abstract：:Actin and myosin are the two main proteins required for cell motility and muscle contraction. The structure of their strongly bound complex-rigor state-is a key for delineating the functional mechanism of actomyosin motor. Current knowledge of that complex is based on models obtained from the docking of known atomic s...

journal_title：Journal of molecular biology

authors： Oztug Durer ZA,Kamal JK,Benchaar S,Chance MR,Reisler E

更新日期：2011-11-25 00:00:00

abstract：:The main regulator of pIP501 replication is an antisense RNA (RNAIII) that induces transcriptional attenuation of the essential RNAII. Previous studies identified the termination point in vivo and demonstrated attenuation in vitro. This in vivo analysis confirms the appearance of attenuated RNAII dependent on RNAIII. ...

journal_title：Journal of molecular biology

authors： Brantl S,Wagner EG

更新日期：1996-01-19 00:00:00

abstract：:Ricin A-chain is an N-glycosidase that hydrolyzes the adenine ring from a specific adenosine of rRNA. Formycin monophosphate (FMP) and adenyl(3'-->5')guanosine (ApG) were bound to ricin A-chain and their structures elucidated by X-ray crystallography. The formycin ring stacks between tyrosines 80 and 123 and at least ...

journal_title：Journal of molecular biology

authors： Monzingo AF,Robertus JD

更新日期：1992-10-20 00:00:00

abstract：:Methyltransferases (MTases) from the Erm family catalyze S-adenosyl-L-methionine-dependent modification of a specific adenine residue in bacterial 23S rRNA, thereby conferring resistance to clinically important macrolide, lincosamide and streptogramin B antibiotics. Despite the available structural data and functional...

journal_title：Journal of molecular biology

authors： Maravić G,Feder M,Pongor S,Flögel M,Bujnicki JM

更新日期：2003-09-05 00:00:00

abstract：:Reverse transcription of HIV-1 genomic RNA to double-stranded DNA by reverse transcriptase (RT) is a critical step in HIV-1 replication. This process relies on two viral proteins, the RT enzyme and nucleocapsid protein NCp7 that has well documented nucleic acid chaperone properties. At the beginning of the linear DNA ...

journal_title：Journal of molecular biology

authors： Beltz H,Azoulay J,Bernacchi S,Clamme JP,Ficheux D,Roques B,Darlix JL,Mély Y

更新日期：2003-04-18 00:00:00

abstract：:Human serum amyloid P component (SAP) is a normal plasma glycoprotein and the precursor of amyloid P component which is a universal constituent of the abnormal tissue deposits in amyloidosis. X-ray and neutron scattering data showed that pentameric or decameric ring structures for SAP in solution are readily distingui...

journal_title：Journal of molecular biology

authors： Ashton AW,Boehm MK,Gallimore JR,Pepys MB,Perkins SJ

更新日期：1997-09-26 00:00:00

abstract：:We examined the effects of neutral salts and the non-ionic solute 2-methyl,-2,4-pentanediol (MPD) on transcript elongation by Escherichia coli RNA polymerase and on pausing induced by the multipartite his leader pause signal. All solutes tested slowed the overall rate of elongation, with anions showing the dominant ef...

journal_title：Journal of molecular biology

authors： Chan CL,Landick R

更新日期：1997-04-25 00:00:00

abstract：:Phosphofructokinase-1 and -2 (Pfk-1 and Pfk-2, respectively) from Escherichia coli belong to different homologous superfamilies. However, in spite of the lack of a common ancestor, they share the ability to catalyze the same reaction and are inhibited by the substrate MgATP. Pfk-2, an ATP-dependent 6-phosphofructokina...

journal_title：Journal of molecular biology

authors： Cabrera R,Ambrosio AL,Garratt RC,Guixé V,Babul J

更新日期：2008-11-14 00:00:00

abstract：:Pertussis toxin is a major virulence factor of Bordetella pertussis, the causative agent of whooping cough. The protein is a hexamer containing a catalytic subunit (S1) that is tightly associated with a pentameric cell-binding component (B-oligomer). In vitro experiments have shown that ATP and a number of detergents ...

journal_title：Journal of molecular biology

authors： Hazes B,Boodhoo A,Cockle SA,Read RJ

更新日期：1996-05-17 00:00:00

abstract：:Signaling photoreceptors mediate diverse organismal adaptations in response to light. As light-gated protein switches, signaling photoreceptors provide the basis for optogenetics, a term that refers to the control of organismal physiology and behavior by light. We establish as novel optogenetic tools the plasmids pDus...

journal_title：Journal of molecular biology

authors： Ohlendorf R,Vidavski RR,Eldar A,Moffat K,Möglich A

更新日期：2012-03-02 00:00:00

abstract：:M-type phospholipase A2 receptor (M-PLA2R) is a member of the mannose receptor family and known as the receptor of secretory phospholipase A2s. It has also been identified as the major autoantigen of idiopathic membranous nephropathy, one of the most common causes for nephrotic syndrome in adults. Here we determine th...

journal_title：Journal of molecular biology

authors： Dong Y,Cao L,Tang H,Shi X,He Y

更新日期：2017-12-08 00:00:00

abstract：:RNA polymerase II encounters various obstacles to transcript elongation both in vivo and in vitro. These include DNA sequence elements and protein bound to the major groove of DNA. Elongation factor SII binds to RNA polymerase II and enables the enzyme to bypass these impediments. SII also activates nascent RNA cleava...

journal_title：Journal of molecular biology

authors： Mote J Jr,Ghanouni P,Reines D

更新日期：1994-02-25 00:00:00

abstract：:A pre-condition for the ab initio assignment of Euler angles to a set of projections from an asymmetric object is that at least three of the available projections correspond to rotations about different axes. For symmetric objects this condition may be relaxed. There are some applications of single-particle electron m...

journal_title：Journal of molecular biology

authors： Patwardhan A,Paul D,Al-Khayat HA,Morris EP

更新日期：2004-11-26 00:00:00

abstract：:A number of beta-sandwich immunoglobulin-like domains have been shown to fold using a set of structurally equivalent residues that form a folding nucleus deep within the core of the protein. Formation of this nucleus is sufficient to establish the complex Greek key topology of the native state. These nucleating residu...

journal_title：Journal of molecular biology

authors： Lappalainen I,Hurley MG,Clarke J

更新日期：2008-01-11 00:00:00

abstract：:Two-dimensional crystalline porin sheets were obtained by reconstitution of monodisperse protein trimers and phospholipids (dimyristoylphosphatidylcholine) by detergent dialysis, analogous to the reconstitution method used for functional tests (Schindler & Rosenbusch, 1981). Three different packing arrangements were o...

journal_title：Journal of molecular biology

authors： Dorset DL,Engel A,Häner M,Massalski A,Rosenbusch JP

更新日期：1983-04-25 00:00:00

abstract：:Actin polymerization plays a major role in cell movement. The controls of actin sequestration/desequestration and of filament turnover are two important features of cell motility. Actin binding proteins use properties derived from the steady-state monomer-polymer cycle of actin in the presence of ATP, to control the F...

journal_title：Journal of molecular biology

authors： Carlier MF,Pantaloni D

更新日期：1997-06-20 00:00:00

abstract：:The TOPRIM domain found in many nucleotidyl transferases contains a DxD motif involved in magnesium ion coordination for catalysis. Medium- to high-copy-number plasmid clones of Yersinia pestis topoisomerase I (YpTOP) with Asp-to-Asn substitution at the first aspartate residue (D117N) of this motif could not be genera...

journal_title：Journal of molecular biology

authors： Cheng B,Annamalai T,Sorokin E,Abrenica M,Aedo S,Tse-Dinh YC

更新日期：2009-01-16 00:00:00

abstract：:We report here the structure of BstYI, an "intermediate" type II restriction endonuclease with overlapping sequence specificities to BamHI and BglII. BstYI, a thermophilic endonuclease, recognizes and cleaves the degenerate hexanucleotide sequence 5'-RGATCY-3' (where R=A or G and Y=C or T), cleaving DNA after the 5'-R...

journal_title：Journal of molecular biology

authors： Townson SA,Samuelson JC,Vanamee ES,Edwards TA,Escalante CR,Xu SY,Aggarwal AK

更新日期：2004-05-07 00:00:00

abstract：:Long-standing questions on how peptides fold are addressed by the simulation at different temperatures of the reversible folding of a peptide in solution in atomic detail. Molecular dynamics simulations correctly predict the structure that is thermodynamically stable at 298 K, irrespective of the initial peptide confo...

journal_title：Journal of molecular biology

authors： Daura X,Jaun B,Seebach D,van Gunsteren WF,Mark AE

更新日期：1998-07-31 00:00:00

abstract：:The three-dimensional structure of ferric myoglobin from the mollusc Aplysia limacina has been refined at 2 X 0 A resolution. The crystallographic R factor, calculated at this stage, is 0 X 194. Despite its high content of apolar residues (both aromatic and aliphatic), Aplysia limacina myoglobin, which contains only o...

journal_title：Journal of molecular biology

authors： Bolognesi M,Coda A,Gatti G,Ascenzi P,Brunori M

更新日期：1985-05-05 00:00:00

abstract：:Toxin-antitoxin systems consist of a stable toxin, frequently with endonuclease activity, and a small, labile antitoxin, which sequesters the toxin into an inactive complex. Under unfavorable conditions, the antitoxin is degraded, leading to activation of the toxin and resulting in growth arrest, possibly also in bact...

journal_title：Journal of molecular biology

authors： Schmidt O,Schuenemann VJ,Hand NJ,Silhavy TJ,Martin J,Lupas AN,Djuranovic S

更新日期：2007-09-28 00:00:00

abstract：:Three homology models of the human ghrelin receptor (GHS-R1a) have been generated from the available X-ray structures of rhodopsin (RHO model), opsin (OPS model) and beta-2 adrenergic receptor (B2 model). The latter was used as a starting point for combined molecular dynamics simulation (MDS) and full atom normal mode...

journal_title：Journal of molecular biology

authors： Floquet N,M'Kadmi C,Perahia D,Gagne D,Bergé G,Marie J,Banères JL,Galleyrand JC,Fehrentz JA,Martinez J

更新日期：2010-01-29 00:00:00

abstract：:Although chemical signaling during embryogenesis is readily addressed by a plethora of available techniques, the developmental functions of ionic signaling are still poorly understood. It is increasingly realized that bioelectric events in nonneural cells are critical for pattern regulation, but their study has been h...

journal_title：Journal of molecular biology

authors： McMillen P,Novak R,Levin M

更新日期：2020-01-17 00:00:00

abstract：:Loss-of-function mutations in the gene encoding the multifunctional protein, DJ-1, have been implicated in the pathogenesis of early-onset familial Parkinson's disease (PD), suggesting that DJ-1 may act as a neuroprotectant for dopaminergic (DA) neurons. Enhanced autophagy may benefit PD by clearing damaged organelles...

journal_title：Journal of molecular biology

authors： Gao H,Yang W,Qi Z,Lu L,Duan C,Zhao C,Yang H

更新日期：2012-10-19 00:00:00

abstract：:Native-state amide hydrogen exchange (HX) of proteins in the presence of denaturant has provided valuable details on the structures of equilibrium folding intermediates. Here, we extend HX theory to model thiol group exchange (SX) in single cysteine-containing variants of sperm whale ferric aquomyoglobin. SX is comple...

journal_title：Journal of molecular biology

authors： Feng Z,Butler MC,Alam SL,Loh SN

更新日期：2001-11-16 00:00:00

abstract：:The process of heat denaturation of recombinant factor XIII (rFXIII), as well as its C-terminal 24 kDA and 12 kDa elastase-produced fragments starting at Ser514 and Thr628, respectively, was investigated in a wide range of conditions by fluorescence, CD and differential scanning calorimetry (DSC). It was found that th...

journal_title：Journal of molecular biology

authors： Kurochkin IV,Procyk R,Bishop PD,Yee VC,Teller DC,Ingham KC,Medved LV

更新日期：1995-04-28 00:00:00

abstract：:The X-ray crystal structure of the sulfide derivative of ferric Lucina pectinata hemoglobin component I (HbI) has been determined at 1.9 A resolution (R-factor 0.186). The heme pocket structural organization of HbI is in keeping with its ligand binding properties. The fast sulfide association rate constant can be rela...

journal_title：Journal of molecular biology

authors： Rizzi M,Wittenberg JB,Coda A,Ascenzi P,Bolognesi M

更新日期：1996-04-26 00:00:00

abstract：:The Monte Carlo estimate of the p value of the hypergeometric test is described and advocated for the testing of the hypothesis that different treatments induce the same mutational spectrum. The hypergeometric test is a generalization of Fisher's "exact" test for tables with more than two rows and two columns. Use of ...

journal_title：Journal of molecular biology

authors： Adams WT,Skopek TR

更新日期：1987-04-05 00:00:00