Abstract:
BACKGROUND:Identification of early megakaryocyte (MK) precursors is difficult and conflicting. The prevalence of leukemias with a megakaryocytic component may be underestimated when conventional techniques are utilized. The recent development of immuno-ultrastructural procedures for simultaneous detection of platelet peroxidase reaction and specific platelet membrane antigens has increased our capability of recognizing blast cells of the megakaryocytic lineage. METHODS:In this study the occurrence and distribution of megakaryoblastic in the FAB subtypes of adult "de novo" acute myeloid leukemias was employed to detect megakaryocytic precursors. RESULTS:A megakaryoblastic proliferation was demonstrated in 8/42 cases (19%), with a much higher percentage than previously reported and an unusual distribution in the M2 subtype. CONCLUSIONS:These findings indicate that megakaryocytic involvement may be found rather frequently when an immunocytochemical ultrastructural method is applied to the characterization of leukemias.
journal_name
Haematologicajournal_title
Haematologicaauthors
Tomasi P,Tallarico A,Vasi V,Scapoli G,Castoldi Gsubject
Has Abstractpub_date
1993-03-01 00:00:00pages
95-100issue
2eissn
0390-6078issn
1592-8721journal_volume
78pub_type
杂志文章相关文献
HAEMATOLOGICA文献大全abstract::T-cell acute lymphoblastic leukemia (T-ALL) patients frequently display NOTCH1 activating mutations and Notch can transcriptionally down-regulate the tumor suppressor PTEN. However, it is not clear whether NOTCH1 mutations associate with decreased PTEN expression in primary T-ALL. Here, we compared patients with or wi...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2009.011999
更新日期:2010-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Most secreted proteins, including coagulation factor X (FX), are synthesized with a signal peptide, which is necessary for targeting the nascent polypeptide into the endoplasmic reticulum. Characterization of naturally occurring mutations may provide insights into the functional roles of the a...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::The recent identification of acquired mutations in key components of the spliceosome machinery strongly implicates abnormalities of mRNA splicing in the pathogenesis of myelodysplastic syndromes. However, questions remain as to how these aberrations functionally combine with the growing list of mutations in genes invo...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2012.075325
更新日期:2013-07-01 00:00:00
abstract::The risk of developing premature ovarian failure and azoospermia is a major concern in long-term survivors treated for Hodgkin's lymphoma. Alkylating chemotherapy containing procarbazine and/or cyclophosphamide causes prolonged azoospermia in 90-100% of men and premature ovarian failure in 5-25% of women under the age...
journal_title:Haematologica
pub_type: 杂志文章,评审
doi:10.3324/haematol.2011.045856
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:The increasing application of multi-color flow cytometry assays for staging and follow-up in mantle cell lymphoma necessitates that the specificity and sensitivity of this technique are evaluated. Data from prospective clinical trials comparing the clinical applicability of flow cytometry to routine diagnost...
journal_title:Haematologica
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.3324/haematol.11267
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The diagnosis of T-cell neoplasia requires the use of immunohistochemistry on tumor sections or molecular genetic analysis of T-cell receptor (TCR) clonality. Multiplex polymerase chain reactions (PCR) offer a sensitive and expeditious approach to determining clonality early in the diagnostic ...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2005-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The rate of post-operative asymptomatic deep-vein thrombosis in Asian patients remains uncertain. The aim of this study was to estimate the rate of venous thromboembolism, including asymptomatic deep-vein thrombosis, undergoing hip or knee surgery and not receiving pharmacological thromboproph...
journal_title:Haematologica
pub_type: 杂志文章,多中心研究
doi:10.3324/haematol.10819
更新日期:2007-09-01 00:00:00
abstract::In contrast to well-established hierarchical concepts of tumor stem cells, leukemia-initiating cells in B-cell precursor acute lymphoblastic leukemia have not yet been phenotypically identified. Different subpopulations, as defined by surface markers, have shown equal abilities to reconstitute leukemia upon transplant...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2017.167502
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The role of hematopoietic chimerism after allogeneic stem cell transplantation (SCT) for acute leukemia remains controversial. We studied the relationship between hematopoietic chimerism and several prognostic variables on the outcome of SCT in patients with acute leukemia. DESIGN AND METHODS...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2003-07-01 00:00:00
abstract::Anti-CD20-containing chemotherapy regimens have become the standard of care for patients with follicular lymphoma needing cytotoxic therapy. Four randomized trials demonstrated a clinical benefit for patients treated with rituximab. However, no long-term follow up (i.e. > 5 years) of these trials is yet available. Bet...
journal_title:Haematologica
pub_type: 杂志文章,随机对照试验
doi:10.3324/haematol.2012.082412
更新日期:2013-07-01 00:00:00
abstract::One hundred patients with newly diagnosed multiple myeloma (MM) were treated with high-dose chemotherapy followed by single or double autologous stem cell transplantation (ASCT). Up-front treatment with a double ASCT tended to prolong progression-free and overall survival. ...
journal_title:Haematologica
pub_type: 信件
doi:
更新日期:2005-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:NAD(P)H:quinone oxidoreductase 1 (NQO1) is an enzyme that protects cells against mutagenicity from free radicals and toxic oxygen metabolites. The gene coding for NQO1 is subject to a genetic polymorphism at nucleotide position 609 (C-->T) of the human NQO1 cDNA. Heterozygous individuals (C/T)...
journal_title:Haematologica
pub_type: 杂志文章,多中心研究
doi:
更新日期:2004-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Identification and characterization of hematopoietic stem cells in peripheral blood (PB) and cord blood (CB) have suggested feasible alternatives to conventional allogeneic bone marrow (BM) transplantation. The growing interest in this use of allogeneic stem cells has prompted the Working Group...
journal_title:Haematologica
pub_type: 杂志文章,评审
doi:
更新日期:1997-03-01 00:00:00
abstract::The control of mRNA stability plays a central role in orchestrating gene-regulatory networks in hematopoietic cell growth, differentiation and tumorigenesis. HNRNPA0, which encodes an RNA-binding protein shown to regulate transcript stability via binding to the AU-rich elements of mRNAs, is located within the commonly...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2013.098657
更新日期:2014-06-01 00:00:00
abstract::Macaques are emerging as a critical animal model in transfusion medicine, because of their evolutionary similarity to humans and perceived utility in discovery and translational science. However, little is known about the metabolism of Rhesus macaque red blood cells (RBC) and how this compares to human RBC metabolism ...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2019.229930
更新日期:2020-08-01 00:00:00
abstract::We confirm a decrease in cobalamins during pregnancy, and report that the active part of cobalamins (holotranscobalamin, holoTC) remains unchanged. The decrease in cobalamins is explained by a decreased holohaptocorrin (holoHC), suggesting that holoTC rather than cobalamins should be used as a marker of vitamin B12 de...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.11636
更新日期:2007-12-01 00:00:00
abstract::A case of cholesterol embolism of bone marrow, concerning the pelvis and lumbar region and clinically masquerading as systemic disease or metastatic tumor, is reported in an 82-year-old man hospitalized for acute onset of reddish purple nodules on the legs and toes, intense myalgia and dorsal vertebral bone pain. The ...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::The assessment of clinical probability represents an important step in the diagnostic strategy of patients with suspected deep vein thrombosis. The recently derived LEFt clinical prediction rule for pregnant women combines three variables: symptoms in the left leg (L), calf circumference difference of 2 centimeters or...
journal_title:Haematologica
pub_type: 临床试验,杂志文章,多中心研究
doi:10.3324/haematol.2012.072009
更新日期:2013-04-01 00:00:00
abstract::Sixteen patients with stage III multiple myeloma (MM) and a median age of 51 years were treated with autografting followed by reduced intensity conditioning allotransplantation (RICT). Nine patients are alive in remission at a median of 30 months after their transplants, one patient is alive in relapse and 6 patients ...
journal_title:Haematologica
pub_type: 临床试验,信件
doi:
更新日期:2004-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The limited value of qualitative reverse transcription polymerase chain-reaction (RT-PCR) for monitoring chronic myeloid leukemia (CML) patients has prompted the development of quantitative assays. We have developed a quantitative real-time PCR (QC-PCR) method in the LightCycler, based on the ...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::Circulating stem cells (CSC) are well documented in animals and humans. Though their function in normal conditions remains obscure, autologous CSC seem capable of restoring hemopoiesis after myeloablative treatment. With cell separators CSC may be harvested in adequate number, and collection may be further improved gi...
journal_title:Haematologica
pub_type: 杂志文章,评审
doi:
更新日期:1990-11-01 00:00:00
abstract:BACKGROUND:Immunoglobulin gene somatic hypermutation is a biologically relevant and clinically useful prognostic factor in different types of low-grade B-cell lymphomas, including chronic lymphocytic leukemia, mantle cell lymphoma and splenic marginal zone lymphoma. DESIGN AND METHODS:With the aim of identifying surro...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.12999
更新日期:2008-08-01 00:00:00
abstract::We report on the likely mechanism of an exacerbation of neurological symptoms developed during immune reconstitution after autologous non-myeloablative hematopoietic stem cell transplantation in a 33-year-old man with systemic lupus erythematosus- associated recurrent transverse myelitis. Cerebrospinal fluid examinati...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00
abstract::A pilot study, testing two different chemotherapeutic schedules in CML patients, was carried out in order to verify the possibility of Ph1 clone disappearance. Both therapeutic regimens, though able to induce severe marrow aplasia, determined only a reduction of Ph1 positive metaphases in two patients, while in one ca...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1989-09-01 00:00:00
abstract:BACKGROUND:The mdr-1 gene, which codes for a 170-kd transmembrane glycoprotein (P170), is frequently overexpressed in multidrug resistant (MDR) tumor cell lines and in spontaneous tumors, including leukemia and lymphoma. However, it is also constitutively expressed as a normal gene in normal tissues. METHODS:We used h...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Chromosome 5 and/or 7 abnormalities are cytogenetic findings indicative of a poor prognosis in patients with acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS). The only potential cure for such patients is allogeneic stem cell transplantation (SCT). As data on allogeneic SCT in thi...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2005-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:To assess the efficacy and the toxic profile of gemcitabine, a novel pyrimidine antimetabolite active against several solid tumors, we carried out a study in heavily pretreated Hodgkin's disease (HD) patients. DESIGN AND METHODS:From May 1997 to January 1999, 14 pretreated patients (10 relaps...
journal_title:Haematologica
pub_type: 临床试验,杂志文章
doi:
更新日期:2000-09-01 00:00:00
abstract::Krüppel-like factor 4 is a transcription factor with anti-proliferative effects in differentiated cells, but with the ability to reprogram adult cells into cell-cycling pluripotent cells. In cancer, Krüppel-like factor 4 acts as either an anti-oncogene or an oncogene. We analyzed Krüppel-like factor 4 gene expression ...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2012.066944
更新日期:2013-09-01 00:00:00
abstract:PURPOSE:Various experiences show no substantial differences between children and adults in the treatment of Hodgkin's disease. In consideration of some peculiar characteristics of these cases which might influence the therapeutical choice, particularly regarding long-term survival and therapeutical complications, we re...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:We investigated outcomes in a UK neonatal cohort as a benchmark for care of children with sickle cell disease (SCD). DESIGN AND METHODS:Two-hundred and fifty-two children (180 with hemoglobin [Hb] SS, 64 with HbSC, and 8 with HbS/beta thalassemia), identified during 1983-2005 by universal bir...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.10937
更新日期:2007-07-01 00:00:00