当前位置： SCI文献检索 > JOURNAL OF MOLECULAR BIOLOGY期刊下所有文献 > Folding pathway mediated by an intramolecular chaperone: characterization of the structural changes in pro-subtilisin E coincident with autoprocessing.

Folding pathway mediated by an intramolecular chaperone: characterization of the structural changes in pro-subtilisin E coincident with autoprocessing.

Abstract：

:Mechanisms by which many N-terminal propeptides facilitate folding of proteins are unknown. The maturation of such proteins from their precursors involve three steps, namely: (1) folding of the precursor, (2) autoprocessing of the propeptide from the N terminus and (3) degradation of the cleaved propeptide. Using subtilisin E we have analyzed the mechanism of propeptide-mediated protein folding. Two active site mutations allow us to trap intermediates at stages of autoprocessing and degradation. An analysis of these intermediates has shown the existence of a molten-globule-like intermediate on the folding pathway. After autoprocessing of the propeptide, this intermediate undergoes a structural reorganization which reduces solvent-accessible hydrophobic surface area and increases the amount of its tertiary structure. Removal of the propeptide from the mature enzyme in this intermediate state occurs only by proteolytic degradation and contributes to the stability of the active enzyme.

journal_name

J Mol Biol

journal_title

Journal of molecular biology

authors

Shinde U,Inouye M

doi

10.1006/jmbi.1995.0472

subject

Has Abstract

pub_date

1995-09-08 00:00:00

pages

25-30

issue

1

eissn

0022-2836

issn

1089-8638

pii

S0022-2836(85)70472-X

journal_volume

252

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR BIOLOGY文献大全
  • Structural properties of a collagenous heterotrimer that mimics the collagenase cleavage site of collagen type I.

    abstract：:Collagens contain sequence- and conformation-dependent epitopes responsible for their digestion by collagenases at specific loci. A synthetic heterotrimer construct containing the collagenase cleavage site of collagen type I was found to mimic perfectly native collagen in terms of selectivity and mode of enzymatic deg...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/S0022-2836(02)00365-0

    authors： Fiori S,Saccà B,Moroder L

    更新日期：2002-06-21 00:00:00

  • Regulatory sequences in sigma 54 localise near the start of DNA melting.

    abstract：:Transcription initiation by the enhancer-dependent sigma(54) RNA polymerase holoenzyme is positively regulated after promoter binding. The promoter DNA melting process is subject to activation by an enhancer-bound activator protein with nucleoside triphosphate hydrolysis activity. Tethered iron chelate probes attached...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.4393

    authors： Wigneshweraraj SR,Chaney MK,Ishihama A,Buck M

    更新日期：2001-03-02 00:00:00

  • Zooming in on the hydrophobic ridge of H-2D(b): implications for the conformational variability of bound peptides.

    abstract：:Class I major histocompatibility complex (MHC) molecules, which display intracellularly processed peptides on the cell surface for scanning by T-cell receptors (TCRs), are extraordinarily polymorphic. MHC polymorphism is believed to result from natural selection, since individuals heterozygous at the corresponding loc...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.5016

    authors： Ciatto C,Tissot AC,Tschopp M,Capitani G,Pecorari F,Plückthun A,Grütter MG

    更新日期：2001-10-05 00:00:00

  • The crystal structures of trout Hb I in the deoxy and carbonmonoxy forms.

    abstract：:We have determined the X-ray crystallographic structure of trout Hb I in both the deoxy and carbonmonoxy forms to resolution limits of 2.3 angstroms and 2.5 angstroms, respectively. The overall fold of the molecule is highly similar to that of human HbA despite the low level of sequence identity between these proteins...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0355

    authors： Tame JR,Wilson JC,Weber RE

    更新日期：1996-06-21 00:00:00

  • Undesigned selection for replication termination of bacterial chromosomes.

    abstract：:The oriC DNA replication origin in bacterial chromosomes, the location of which appears to be physically identified, is genetically regulated by relevant molecular machinery. In contrast, the location of the terminus remains obscure for many bacterial replicons, except for terC, the proposed and well-studied chromosom...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2014.06.005

    authors： Kono N,Arakawa K,Sato M,Yoshikawa H,Tomita M,Itaya M

    更新日期：2014-08-12 00:00:00

  • Premature stop codons inactivate the RT6 genes of the human and chimpanzee species.

    abstract：:RT6 is a glycosyl phosphatidylinositol-anchored cell membrane protein, whose expression is restricted to peripheral T cells and intraepithelial lymphocytes. It has attracted interest as a T cell differentiation marker and activation antigen in rats. The only known protein to which RT6 shows significant homology is a r...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1680

    authors： Haag F,Koch-Nolte F,Kühl M,Lorenzen S,Thiele HG

    更新日期：1994-10-28 00:00:00

  • Effect of cytosine methylation on DNA-DNA recognition at CpG steps.

    abstract：:Although DNA methylation is a fundamental mechanism for repressing genetic activity, the influence of methyl groups on DNA conformation is found to be small. In this study, the role of cytosine methylation is analysed in the context of DNA condensation by examining its influence on DNA-DNA recognition processes. Previ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1997.1137

    authors： Mayer-Jung C,Moras D,Timsit Y

    更新日期：1997-07-18 00:00:00

  • Transition between different binding modes in rat DNA polymerase beta-ssDNA complexes.

    abstract：:Interactions of rat DNA polymerase beta with a single-stranded (ss) DNA have been studied using the quantitative fluorescence titration technique. Examination of the fluorescence changes accompanying the binding, as a function of the thermodynamically rigorous binding density of rat pol beta-ssDNA complexes, reveals t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1998.2252

    authors： Jezewska MJ,Rajendran S,Bujalowski W

    更新日期：1998-12-11 00:00:00

  • A pentavalent single-domain antibody approach to tumor antigen discovery and the development of novel proteomics reagents.

    abstract：:Proteomics research has delivered many novel tumor targets. However, due to key limitations, it does not specifically identify targets that are most accessible for drug delivery, such as cell-surface antigens. A novel tumor antigen discovery platform based on screening a single domain antibody (sdAb) library against t...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.05.069

    authors： Zhang J,Li Q,Nguyen TD,Tremblay TL,Stone E,To R,Kelly J,Roger MacKenzie C

    更新日期：2004-07-30 00:00:00

  • Negatively stained 50 S ribosomal subunits of Escherichia coli.

    abstract：:Ribosomes are large nucleoproteins of approximately 3 X 10(6) Mr. In contrast to helical or spherical nucleoproteins (viruses) of similar size (which consist of several hundred small asymmetric units reproduced by symmetry), ribosomes are completely asymmetric; therefore, the amount of structural information (defined ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(86)90366-9

    authors： Hoppe W,Oettl H,Tietz HR

    更新日期：1986-11-20 00:00:00

  • Structural biology sheds light on the puzzle of genomic ORFans.

    abstract：:Genomic ORFans are orphan open reading frames (ORFs) with no significant sequence similarity to other ORFs. ORFans comprise 20-30% of the ORFs of most completely sequenced genomes. Because nothing can be learnt about ORFans via sequence homology, the functions and evolutionary origins of ORFans remain a mystery. Furth...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.06.073

    authors： Siew N,Fischer D

    更新日期：2004-09-10 00:00:00

  • Structural changes in tubulin sheets upon removal of microtubule-associated proteins.

    abstract：:Zinc-induced tubulin sheets without microtubule-associated proteins (MAPs) were assembled from tubulin purified by phosphocellulose chromatography. Large, open sheets were obtained in five-minute incubations at pH 5.7. Electron micrographs of negatively stained sheets showed a protofilament arrangement similar to that...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(83)80002-3

    authors： McEwen BF,Ceska TA,Crepeau RH,Edelstein SJ

    更新日期：1983-05-15 00:00:00

  • Solution structure of a C-terminal coiled-coil domain from bovine IF(1): the inhibitor protein of F(1) ATPase.

    abstract：:Bovine IF(1) is a basic, 84 amino acid residue protein that inhibits the hydrolytic action of the F(1)F(0) ATP synthase in mitochondria under anaerobic conditions. Its oligomerization state is dependent on pH. At a pH value below 6.5 it forms an active dimer. At higher pH values, two dimers associate to form an inacti...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2001.4570

    authors： Gordon-Smith DJ,Carbajo RJ,Yang JC,Videler H,Runswick MJ,Walker JE,Neuhaus D

    更新日期：2001-04-27 00:00:00

  • Transcriptional control of IS1 transposition in Escherichia coli.

    abstract：:The movement of the bacterial insertion sequence IS1 often generates cointegrate structures in which donor and target replicons are connected by direct repeats of IS1. The experiments reported here were designed to understand how IS1 transposition is controlled. Our physical characterization of the structures of coint...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(84)90337-1

    authors： Biel SW,Adelt G,Berg DE

    更新日期：1984-04-05 00:00:00

  • Folding mechanism of indole-3-glycerol phosphate synthase from Sulfolobus solfataricus: a test of the conservation of folding mechanisms hypothesis in (beta(alpha))(8) barrels.

    abstract：:As a test of the hypothesis that folding mechanisms are better conserved than sequences in TIM barrels, the equilibrium and kinetic folding mechanisms of indole-3-glycerol phosphate synthase (sIGPS) from the thermoacidophilic archaebacterium Sulfolobus solfataricus were compared to the well-characterized models of the...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(02)00557-0

    authors： Forsyth WR,Matthews CR

    更新日期：2002-07-26 00:00:00

  • A conserved unfoldase activity for the p97 AAA-ATPase in proteasomal degradation.

    abstract：:The multifunctional AAA-ATPase p97 is one of the most abundant and conserved proteins in eukaryotic cells. The p97/Npl4/Ufd1 complex dislocates proteins that fail the protein quality control in the endoplasmic reticulum to the cytosol where they are subject to degradation by the ubiquitin/proteasome system. Substrate ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.09.050

    authors： Beskow A,Grimberg KB,Bott LC,Salomons FA,Dantuma NP,Young P

    更新日期：2009-12-11 00:00:00

  • Human prorenin structure sheds light on a novel mechanism of its autoinhibition and on its non-proteolytic activation by the (pro)renin receptor.

    abstract：:Antibodies and prorenin mutants have long been used to structurally characterize prorenin, the inactive proenzyme form of renin. They were designed on the basis of homology models built using other aspartyl protease proenzyme structures since no structure was available for prorenin. Here, we present the first X-ray st...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2012.05.003

    authors： Morales R,Watier Y,Böcskei Z

    更新日期：2012-08-03 00:00:00

  • Effects of experimentally achievable improvements in the quality of NMR distance constraints on the accuracy of calculated protein structures.

    abstract：:New methods for collecting cross-relaxation data from proteins and nucleic acids make it possible to improve the accuracy and precision of interproton distance measurements used as input for NMR solution structure determinations. It thus is of interest to determine whether such experimentally achievable improvements i...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1996.0254

    authors： Hoogstraten CG,Markley JL

    更新日期：1996-05-03 00:00:00

  • A conserved insertion in protein-primed DNA polymerases is involved in primer terminus stabilisation.

    abstract：:Protein-primed DNA polymerases form a subgroup of the eukaryotic-type DNA polymerases family, also called family B or alpha-like. A multiple amino acid sequence alignment of this subgroup of DNA polymerases led to the identification of two insertions, TPR-1 and TPR-2, in the polymerisation domain. We showed previously...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00788-5

    authors： Dufour E,Rodríguez I,Lázaro JM,de Vega M,Salas M

    更新日期：2003-08-22 00:00:00

  • Observing membrane protein diffusion at subnanometer resolution.

    abstract：:Single sodium-driven rotors from a bacterial ATP synthase were embedded into a lipid membrane and observed in buffer solution at subnanometer resolution using atomic force microscopy (AFM). Time-lapse AFM topographs show the movement of single proteins within the membrane. Subsequent analysis of their individual traje...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/s0022-2836(03)00206-7

    authors： Müller DJ,Engel A,Matthey U,Meier T,Dimroth P,Suda K

    更新日期：2003-04-11 00:00:00

  • The human homologue of the yeast splicing factor prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions.

    abstract：:An essential step of pre-mRNA spliceosome assembly is the interaction between the snRNPs U4/U6 and U5, to form the [U4/U6.U5] tri-snRNP. While the tri-snRNP protein Prp6p appears to play an important role for tri-snRNP formation in yeast, little is known about the interactions that connect the two snRNP particles in h...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.2000.3685

    authors： Makarov EM,Makarova OV,Achsel T,Lührmann R

    更新日期：2000-05-12 00:00:00

  • What drives proteins into the major or minor grooves of DNA?

    abstract：:The energetic profiles of a significant number of protein-DNA systems at 20 degrees C reveal that, despite comparable Gibbs free energies, association with the major groove is primarily an enthalpy-driven process, whereas binding to the minor groove is characterized by an unfavorable enthalpy that is compensated by fa...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jmb.2006.09.059

    authors： Privalov PL,Dragan AI,Crane-Robinson C,Breslauer KJ,Remeta DP,Minetti CA

    更新日期：2007-01-05 00:00:00

  • Non-receptor tyrosine kinases c-Abl and Arg regulate the activity of C/EBPbeta.

    abstract：:The CCAAT/enhancer-binding protein beta (C/EBPbeta) is a critical transcription factor that regulates gene expression during numerous biological processes, including differentiation, metabolism, homeostasis, proliferation, tumorigenesis, inflammation, and apoptosis. In this study, interactions between C/EBPbeta and ei...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2009.06.055

    authors： Li X,Liu X,Wang G,Zhu X,Qu X,Li X,Yang Y,Peng L,Li C,Li P,Huang W,Ma Q,Cao C

    更新日期：2009-08-28 00:00:00

  • Diversity of molecular transformations involved in the formation of spider silks.

    abstract：:Spiders that spin orb webs secrete seven types of silk. Although the spinning process of the dragline thread is beginning to be understood, the molecular events that occur in spiders' opisthosomal glands, which produce the other fibers, are unknown due to a lack of data regarding their initial and final structures. Ta...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2010.10.052

    authors： Lefèvre T,Boudreault S,Cloutier C,Pézolet M

    更新日期：2011-01-07 00:00:00

  • Sites of reaction of Escherichia coli DNA gyrase on pBR322 in vivo as revealed by oxolinic acid-induced plasmid linearization.

    abstract：:pBR322 DNA, linearized by lysis of an oxolinic acid-treated culture of Escherichia coli strain DK6recA- (pBR322) with sodium dodecyl sulfate, was purified, treated with DNA polymerase in the presence of the four deoxynucleoside triphosphates, and ligated to DNA linkers containing the XhoI recognition sequence. Most of...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(85)90324-9

    authors： Lockshon D,Morris DR

    更新日期：1985-01-05 00:00:00

  • Crystal structure of the Drosophila peptidoglycan recognition protein (PGRP)-SA at 1.56 A resolution.

    abstract：:Peptidoglycan recognition proteins (PGRPs) form a recently discovered protein family, which is conserved from insect to mammals and is implicated in the innate immune system by interacting with/or degrading microbial peptidoglycans (PGNs). Drosophila PGRP-SA is a member of this family of pattern recognition receptors ...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2004.04.077

    authors： Reiser JB,Teyton L,Wilson IA

    更新日期：2004-07-16 00:00:00

  • RNA binding, packaging and polymerase activities of the different incomplete polymerase complex particles of dsRNA bacteriophage phi 6.

    abstract：:phi 6 is an enveloped dsRNA bacterial virus. Its segmented genome resides inside the virion associated polymerase complex which is formed by four proteins (P1, P2, P4 and P7) encoded by the viral L segment. Complete and incomplete polymerase complex particles can be produced using cDNA copies of this largest genome se...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1995.0317

    authors： Juuti JT,Bamford DH

    更新日期：1995-06-09 00:00:00

  • Modular structure in developmentally eliminated DNA in Tetrahymena may be a consequence of frequent insertions and deletions.

    abstract：:The work reported here describes insertion-deletion (Indel) polymorphisms in two internally eliminated sequences (IESs, that are deleted during development in Tetrahymena): a 1.8 kb Indel at one end of the 1.1 kb H1 IES and a 0.5 kb Indel inside the 1.4 kb calmodulin (C) IES. These two IESs are located in the proximit...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/j.jmb.2003.12.065

    authors： Huvos P

    更新日期：2004-03-05 00:00:00

  • Crystallization and preliminary crystallographic analysis of a DNA (cytosine-5)-methyltransferase from Haemophilus aegyptius bound covalently to DNA.

    abstract：:A DNA (cytosine)-5-methyltransferase from Haemophilus aegyptius (M.Hae III), which catalyzes methyl transfer from S-adenosyl-L-methionine to DNA, has been crystallized as a covalent complex with a suicide oligonucleotide substrate. Crystals of the co-complex were grown by vapor diffusion with hanging droplets, using p...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1006/jmbi.1994.1319

    authors： Reinisch KM,Chen L,Verdine GL,Lipscomb WN

    更新日期：1994-05-13 00:00:00

  • Erythromycin-induced stabilization of ermA messenger RNA in Staphylococcus aureus and Bacillus subtilis.

    abstract：:Erythromycin-induced stabilization of ermA mRNA was studied in Staphylococcus aureus, its original host background, and in Bacillus subtilis, subcloned on plasmid vectors. By RNA blot analysis it was shown that 40 nM-erythromycin specifically increased the chemical half-life of ermA mRNA from 2.5 to 17.5 minutes where...

    journal_title：Journal of molecular biology

    pub_type： 杂志文章

    doi：10.1016/0022-2836(88)90116-7

    authors： Sandler P,Weisblum B

    更新日期：1988-10-20 00:00:00