The time course of myosin light-chain phosphorylation in blood-induced vasospasm.

Abstract:

:The phosphorylation of an M(r) 20,000 myosin light chain (MLC20) promotes the generation of contractile force through actin-myosin adenosine triphosphatase in most agonist-mediated vascular smooth muscle cell contraction. However, the role of calcium-mediated contractile processes in sustained arterial narrowing after subarachnoid hemorrhage remains unknown. In a femoral artery model of vasospasm, whole blood was applied to arteries in 54 rats for periods of 2 to 10 days; the contralateral artery treated with platelet-rich plasma served as matched control. During the early stage of vasospasm (Days 2-5), in the media of arteries exposed to blood, MLC20 phosphorylation (including diphosphorylated forms) increased significantly (30-38%; P < 0.05); total medial MLC20 during this interval was comparable to that in controls. After 5 days, however, total MLC20 decreased markedly (> 90%; P < 0.01) compared with controls; phosphorylated MLC20 was undetectable during this interval. MLC20-mediated contractile processes may be prominent in the early stages of arterial narrowing after subarachnoid hemorrhage; later stages are associated with the loss of MLC20 and the possible persistence of arterial narrowing by other mechanisms.

journal_name

Neurosurgery

journal_title

Neurosurgery

authors

Harada T,Seto M,Sasaki Y,London S,Luo Z,Mayberg M

doi

10.1227/00006123-199506000-00018

subject

Has Abstract

pub_date

1995-06-01 00:00:00

pages

1178-82; discussion 1182-3

issue

6

eissn

0148-396X

issn

1524-4040

journal_volume

36

pub_type

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