Cells transfected with the basic fibroblast growth factor gene fused to a signal sequence are invasive in vitro and in situ in the brain.

Abstract:

:Invasiveness is a critical event in the development of malignancy in brain tumors. A potential molecular mediator is basic fibroblast growth factor (bFGF). NIH-3T3 cells transfected with the bFGF gene fused with a signal peptide sequence (signal peptide bFGF) acquire an invasive phenotype as measured by in vitro assays of invasion including: 1) the formation of branching networks on Matrigel; 2) invasiveness in a chemoinvasion assay; 3) migration in a cell spreading assay; 4) detection of an Mr 92,000 gelatinase; and 5) local invasion into the surrounding neuropil after injection in the athymic mouse brain. By contrast, cells transfected with only the native bFGF gene (wild-type bFGF): 1) formed discrete cell clusters on Matrigel; 2) were less invasive and migratory in vitro; 3) released minimal Mr 92,000 collagenase; and 4) in vivo formed a pseudocapsule that separated the tumor cells from the neuropil. Quantitation of bFGF in the conditioned serum-free medium of the cell lines by enzyme-linked immunosorbent assay demonstrated that the signal peptide-bFGF cell clone secreted bFGF. These findings suggest a role for bFGF-mediated pathways and collagenase as molecular determinants of invasiveness in the brain.

journal_name

Neurosurgery

journal_title

Neurosurgery

authors

Gately S,Tsanaclis AM,Takano S,Klagsbrun M,Brem S

doi

10.1227/00006123-199504000-00020

subject

Has Abstract

pub_date

1995-04-01 00:00:00

pages

780-8

issue

4

eissn

0148-396X

issn

1524-4040

journal_volume

36

pub_type

杂志文章
  • Ranging accuracy test of the sonic microstereometric system.

    abstract::We have recently introduced a new type of ultrasonic-based, three-dimensional ranging system for the localization of small handheld surgical tools to be used during operation in open stereotactic brain surgery. This targeting system has now been extensively laboratory tested during thousands of measuring cycles with a...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/00006123-199404000-00030

    authors: Horstmann GA,Reinhardt HF

    更新日期:1994-04-01 00:00:00

  • Most intracranial meningiomas are not cleavable tumors: anatomic-surgical evidence and angiographic predictibility.

    abstract:OBJECTIVE:The statement that intracranial meningiomas are cleavable tumors has to be seriously questioned from a surgical standpoint. The purpose of this study was 1) to analyze the operative reports of a personal series of meningiomas to evaluate the percentages of the tumors that could be dissected by passing in the ...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1097/00006123-199803000-00007

    authors: Sindou MP,Alaywan M

    更新日期:1998-03-01 00:00:00

  • Cephaloceles: treatment, outcome, and antenatal diagnosis.

    abstract::A series of 74 cephaloceles (17 cranial meningoceles and 57 meningoencephaloceles) is reported. Infants born with large meningoencephaloceles containing recognizable cerebral tissue usually did badly despite endeavors to conserve brain function by expanding the cranial capacity (5 cases) or decompressing hydrocephalic...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/00006123-198407000-00005

    authors: Simpson DA,David DJ,White J

    更新日期:1984-07-01 00:00:00

  • Microplate-bridge technique for watertight dural closures in the combined petrosal approach.

    abstract:BACKGROUND:Although the combined petrosal approach has significant advantages for medium to large petroclival lesions, it carries the risk of a few major complications. The cerebrospinal fluid leak rate with this approach has been reported to be as high as 15%. OBJECTIVE:To describe an innovative technique of watertig...

    journal_title:Neurosurgery

    pub_type: 杂志文章,评审

    doi:10.1227/NEU.0b013e3182356269

    authors: Kusumi M,Fukushima T,Aliabadi H,Mehta AI,Noro S,Rosen CL,Fujii K

    更新日期:2012-06-01 00:00:00

  • The use of susceptibility-weighted imaging as an indicator of retrograde leptomeningeal venous drainage and venous congestion with dural arteriovenous fistula: diagnosis and follow-up after treatment.

    abstract:BACKGROUND:Retrograde leptomeningeal venous drainage (RLVD) in dural arteriovenous fistulas (DAVFs) is associated with intracerebral hemorrhage and nonhemorrhagic neurological deficits or death. Angiographic evidence of RLVD is a definite indication for treatment, but less invasive methods of identifying RLVD are requi...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/NEU.0b013e318276f7cc

    authors: Nakagawa I,Taoka T,Wada T,Nakagawa H,Sakamoto M,Kichikawa K,Hironaka Y,Motoyama Y,Park YS,Nakase H

    更新日期:2013-01-01 00:00:00

  • Cervical artery dissections: evidence for redissection in previously dissected arteries: report of three cases.

    abstract:OBJECTIVE AND IMPORTANCE:Among patients with spontaneous internal carotid artery (ICA) or vertebral artery dissections, there is a small but significant risk of development of dissection in an uninvolved ICA or vertebral artery at some time in the future. Recurrence of dissection in a previously dissected and healed IC...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:

    authors: Atkinson JL,Piepgras DG,Huston J 3rd,Mokri B

    更新日期:2002-09-01 00:00:00

  • Quality of life in patients 2 to 4 years after closed head injury.

    abstract::This study evaluated quality of life in 78 patients with closed head injury (CHI) 2 to 4 years postinjury. Using both interview data and mean data from the Sickness Impact Profile questionnaire, impaired quality of life was observed in the areas of psychosocial functioning, social role functioning, leisure activities,...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/00006123-198611000-00004

    authors: Klonoff PS,Snow WG,Costa LD

    更新日期:1986-11-01 00:00:00

  • Pediatric cerebellar infarction: case report and review of the literature.

    abstract::Infarction in the vertebrobasilar system is uncommon in children. Cerebellar infarction presenting as a posterior fossa mass lesion is distinctly rare. We recently treated a 13-year-old boy with such a lesion as a complication of basilar artery migraine. A review of the literature on vertebrobasilar infarction in chil...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/00006123-198205000-00008

    authors: Harbaugh RE,Saunders RL,Reeves AG

    更新日期:1982-05-01 00:00:00

  • Prevention of chronic cerebral vasospasm in dogs with milrinone.

    abstract:OBJECTIVE:Delayed cerebral ischemia resulting from vasospasm is a major cause of morbidity and death in patients with aneurysmal subarachnoid hemorrhage. Milrinone, because it inhibits Type IV cyclic adenosine monophosphate-specific phosphodiesterase enzyme in both cardiac and vascular smooth muscle, is a powerful inot...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1097/00006123-199702000-00025

    authors: Khajavi K,Ayzman I,Shearer D,Jones SC,Levy JH,Prayson RA,Skibinski CI,Hahn JF,Chyatte D

    更新日期:1997-02-01 00:00:00

  • Telecanthal approach for meningiomas in the ethmoid and sphenoid sinuses.

    abstract::In three cases involving meningiomas in the ethmoid and sphenoid sinuses, transbasal spreading of the interocular distance (telecanthal approach) was used for tumor removal and reconstruction of the skull base. This telecanthal approach involves 1) bilateral en bloc removal of the superior lateral rim of the orbit, th...

    journal_title:Neurosurgery

    pub_type: 杂志文章,评审

    doi:10.1097/00006123-199105000-00013

    authors: Fujitsu K,Saijoh M,Aoki F,Sakata K,Fujii S,Mochimatsu Y,Kuwabara T

    更新日期:1991-05-01 00:00:00

  • Neurogenic pulmonary edema associated with ruptured intracranial aneurysm: case report.

    abstract::A case of ruptured distal anterior cerebral artery aneurysm associated with neurogenic pulmonary edema is presented. It is suggested that this association should not be accepted as a "taboo" for radical intervention followed by a proper management of ventilation with positive end-expiratory pressure to maintain the an...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/00006123-198608000-00025

    authors: Yabumoto M,Kuriyama T,Iwamoto M,Kinoshita T

    更新日期:1986-08-01 00:00:00

  • Hemianopia: a presenting feature of acute epidural hematomas.

    abstract::Visual field abnormalities were studied in a retrospective analysis of 199 patients with epidural hematomas. One hundred three patients had been alert enough at the time of hospital admission to have had their visual fields examined, but in only 27 patients were visual fields documented in the charts. Six of the 27 pa...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/00006123-198208000-00007

    authors: Matwijecky C,Steinbok P

    更新日期:1982-08-01 00:00:00

  • Treatment of cerebrospinal fluid and syringosubarachnoid shunt infection with systemic and intrathecal antibiotics.

    abstract::We present a case of a patient with two preexisting Silastic syringosubarachnoid shunt catheters who developed Enterobacter meningitis. The infection was treated successfully with systemic and intrathecal antibiotics without removal of the catheters. With careful sequential clinical and laboratory monitoring, even thi...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/00006123-198508000-00015

    authors: Tomaszek DE,Powers SK

    更新日期:1985-08-01 00:00:00

  • A special cyst puncture catheter for use in thick-walled or mobile intracranial cysts.

    abstract::Having encountered a number of thick-walled or mobile symptomatic intracranial cysts that have resisted stereotactic puncture with standard blunt-ended ventricular catheters, the authors have designed a cyst puncture catheter that has a number of features helpful in overcoming this problem. The catheter and its use ar...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:

    authors: Ross DA,Muraszko K,Dauser R

    更新日期:1994-01-01 00:00:00

  • Unusual angiographic changes in a dissecting aneurysm of the basilar artery: case report.

    abstract::A patient with unusual angiographic changes of a dissecting aneurysm of the basilar artery is presented. Initial angiography and magnetic resonance imaging revealed a typical dissecting aneurysm. However, a follow-up study demonstrated a saccular aneurysmal dilatation of the proximal part of the dissection. The import...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/00006123-199402000-00022

    authors: Takagi M,Hirata K,Fujitsu K,Yamamoto I

    更新日期:1994-02-01 00:00:00

  • False aneurysm complicating carotid endarterectomy.

    abstract::We present two cases of false aneurysm after carotid endarterectomy with primary arteriotomy closure. In both cases, infection appeared to be the precipitating cause. Both lesions were repaired with a saphenous vein patch graft, but infection in one patient persisted, and recurrence of a false aneurysm required ligati...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/00006123-198609000-00014

    authors: Branch CL Jr,Davis CH Jr

    更新日期:1986-09-01 00:00:00

  • Sir Victor Horsley (1857-1916): pioneer of neurological surgery.

    abstract::Immortalized in surgical history for the introduction of "antiseptic wax," Sir Victor Horsley played a pivotal role in shaping the face of standard neurosurgical practice. His contributions include the first laminectomy for spinal neoplasm, the first carotid ligation for cerebral aneurysm, the curved skin flap, the tr...

    journal_title:Neurosurgery

    pub_type: 传,历史文章,杂志文章

    doi:10.1097/00006123-200203000-00032

    authors: Tan TC,Black PM

    更新日期:2002-03-01 00:00:00

  • Robot-assisted stereotactic laser ablation in medically intractable epilepsy: operative technique.

    abstract:BACKGROUND:Stereotactic laser ablation offers an advantage over open surgical procedures for treatment of epileptic foci, tumors, and other brain pathology. Robot-assisted stereotactic laser ablation could offer an accurate, efficient, minimally invasive, and safe method for placement of an ablation catheter into the t...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/NEU.0000000000000286

    authors: Gonzalez-Martinez J,Vadera S,Mullin J,Enatsu R,Alexopoulos AV,Patwardhan R,Bingaman W,Najm I

    更新日期:2014-06-01 00:00:00

  • Effect of subarachnoid hemorrhage on serotonin uptake and release in the rabbit basilar artery.

    abstract::This study analyzes the changes induced by subarachnoid hemorrhage (SAH) on the serotonin (5-hydroxytryptamine, 5-HT) uptake and release evoked in rabbit basilar arteries by tyramine. Rabbits were injected with 5 ml of autologous arterial blood into the cisterna magna to produce SAH. Tritium accumulation in basilar ar...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:

    authors: Tanaka Y,Machi T,Nihei H,Kassell NF

    更新日期:1991-03-01 00:00:00

  • A preliminary study of the prognostic value of proton magnetic resonance spectroscopic imaging in gamma knife radiosurgery of recurrent malignant gliomas.

    abstract:OBJECTIVE:The goal of this study was to investigate the use of proton magnetic resonance spectroscopic imaging as a prognostic indicator in gamma knife radiosurgery of recurrent gliomas. METHODS:Thirty-six patients with recurrent gliomas were studied with proton magnetic resonance spectroscopic imaging at the time of ...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1097/00006123-200002000-00011

    authors: Graves EE,Nelson SJ,Vigneron DB,Chin C,Verhey L,McDermott M,Larson D,Sneed PK,Chang S,Prados MD,Lamborn K,Dillon WP

    更新日期:2000-02-01 00:00:00

  • Feasibility, safety, and periprocedural complications associated with endovascular treatment of selected ruptured aneurysms under conscious sedation and local anesthesia.

    abstract:BACKGROUND:Endovascular coil embolization of ruptured aneurysms is performed under general anesthesia at most centers for perceived improved image quality and patient safety. OBJECTIVE:To report the feasibility of and outcomes associated with endovascular treatment of subarachnoid hemorrhage (SAH) patients with ruptur...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/NEU.0b013e31827b9183

    authors: Kan P,Jahshan S,Yashar P,Orion D,Webb S,Siddiqui AH,Hopkins LN,Levy EI

    更新日期:2013-02-01 00:00:00

  • Failure of extracranial-intracranial arterial bypass in acute middle cerebral artery occlusion: case report.

    abstract::Extracranial-intracranial arterial bypass procedures provide important augmentation of collateral circulation to cortical areas rendered potentially ischemic by therapeutic occlusion of major branches of the circle of Willis. Although the case studies reported to date in general reflect positively on this use of the b...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:

    authors: Samson DS,Neuwelt EA,Beyer CW,Ditmore QM

    更新日期:1980-02-01 00:00:00

  • The epidemiology of urban pediatric neurological trauma: evaluation of, and implications for, injury prevention programs.

    abstract:OBJECTIVE:To describe the incidence and causes of pediatric head, spinal cord, and peripheral nerve injuries in an urban setting and to assess the implications of these data for injury prevention programs. METHODS:Pediatric deaths and hospital admissions secondary to neurological trauma included in the Northern Manhat...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1097/00006123-199802000-00052

    authors: Durkin MS,Olsen S,Barlow B,Virella A,Connolly ES Jr

    更新日期:1998-02-01 00:00:00

  • History of the Department of Neurosurgery at Thomas Jefferson University Hospital.

    abstract::The neurosurgical tradition at Jefferson Medical College began in the 19th century with Samuel Gross. In his textbook entitled A System of Surgery, Gross revealed his knowledge of the disorders of the nervous system at a time when innovations were practically inexistent. Gross' work paved the way for William Williams ...

    journal_title:Neurosurgery

    pub_type: 历史文章,杂志文章

    doi:10.1227/01.neu.0000430299.02714.46

    authors: Chalouhi N,Osterholm J,Jabbour P,Dumont AS,Gonzalez LF,Harrop J,Sharan A,Rosenwasser R,Tjoumakaris S

    更新日期:2013-10-01 00:00:00

  • Randomized trials of neurosurgical interventions: a systematic appraisal.

    abstract:OBJECTIVE:To systematically appraise the study design and quality of reporting of randomized controlled trials (RCTs) on neurosurgical procedures and to identify potential defects and biases. METHODS:Randomized controlled trials with at least five patients comparing any neurosurgical procedure against another procedur...

    journal_title:Neurosurgery

    pub_type: 杂志文章,评审

    doi:10.1227/01.neu.0000126873.00845.a7

    authors: Vranos G,Tatsioni A,Polyzoidis K,Ioannidis JP

    更新日期:2004-07-01 00:00:00

  • Esophageal perforation during surgery on the cervical spine.

    abstract::Esophageal perforation during surgery for cervical disc herniation is a rare complication. Differences in the time of diagnosis of this complication in three patients--no delay and delays of 6 hours and 4 days, respectively--resulted in different symptoms and outcomes. Early detection of the perforation allows prompt ...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1097/00006123-199111000-00021

    authors: van Berge Henegouwen DP,Roukema JA,de Nie JC,vd Werken C

    更新日期:1991-11-01 00:00:00

  • Significance of the so-called absorptive reserve in communicating hydrocephalus: a preliminary report.

    abstract::Twelve patients with communicating hydrocephalus were studied with a servocontrolled lumbar infusion technique to measure net cerebrospinal fluid (CSF) absorptive capacity and resting pressure. Each patient showed a significant absorptive reserve; the rate of CSF absorption exceeded the rate of formation over a physio...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/00006123-198105000-00003

    authors: Sklar FH,Beyer CW Jr,Diehl JT,Clark WK

    更新日期:1981-05-01 00:00:00

  • Shunt-related abdominal metastasis of cerebral teratocarcinoma: report of an unusual case and review of the literature.

    abstract:OBJECTIVE AND IMPORTANCE:Internal drainage of cerebrospinal fluid to the abdominal cavity via a ventriculoperitoneal shunt (VPS) is a common procedure for therapy of obstructive hydrocephalus; because this condition is often caused by brain tumors blocking the natural cerebrospinal fluid pathways, the VPS as an artific...

    journal_title:Neurosurgery

    pub_type: 杂志文章,评审

    doi:10.1097/00006123-199806000-00118

    authors: Rickert CH,Reznik M,Lenelle J,Rinaldi P

    更新日期:1998-06-01 00:00:00

  • The Levels of Glial Fibrillary Acidic Protein and Ubiquitin C-Terminal Hydrolase-L1 During the First Week After a Traumatic Brain Injury: Correlations With Clinical and Imaging Findings.

    abstract:BACKGROUND:Glial fibrillary acidic protein (GFAP) and ubiquitin C-terminal hydrolase-L1 (UCH-L1) are promising biomarkers of traumatic brain injury (TBI). OBJECTIVE:We investigated the relation of the GFAP and UCH-L1 levels to the severity of TBI during the first week after injury. METHODS:Plasma UCH-L1 and GFAP were...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/NEU.0000000000001226

    authors: Posti JP,Takala RS,Runtti H,Newcombe VF,Outtrim J,Katila AJ,Frantzén J,Ala-Seppälä H,Coles JP,Hossain MI,Kyllönen A,Maanpää HR,Tallus J,Hutchinson PJ,van Gils M,Menon DK,Tenovuo O

    更新日期:2016-09-01 00:00:00

  • Value of sequential computed tomography in the multimodality treatment of glioblastoma multiforme.

    abstract::Previous assessments of the value of sequential computed tomographic (CT) scanning in brain tumor patients have suffered from the heterogeneous nature of the study populations in regard to (a) pathology, (b) treatment plan, and (c) time of scanning. This report is based on the first 21 of 30 consecutive cases of gliob...

    journal_title:Neurosurgery

    pub_type: 杂志文章

    doi:10.1227/00006123-198101000-00004

    authors: Salcman M,Levine H,Rao K

    更新日期:1981-01-01 00:00:00