A rosette assay for identification of Ia-like alloantigens on chicken lymphoid cells.

abstract：:Drugs that target tumor necrosis factor-alpha (TNF) are particularly important in the treatment of severe inflammatory progression in rheumatoid arthritis, Crohn's disease and psoriasis. Despite the central role of the TNF/TNF receptor (TNFR) in various disease states, there is a paucity of information concerning TNFR...

journal_title：Journal of immunological methods

authors： Abe Y,Yoshikawa T,Kamada H,Shibata H,Nomura T,Minowa K,Kayamuro H,Katayama K,Miyoshi H,Mukai Y,Yoshioka Y,Nakagawa S,Tsunoda S,Tsutsumi Y

更新日期：2008-06-01 00:00:00

abstract：:Pretreatment of polystyrene microplate wells with certain doses of UV light enhances their capacity for binding to single-stranded DNA, double stranded DNA and various synthetic polynucleotides. The use of UV-irradiated plates to immobilize nucleic acid antigens provides a simple, rapid, and specific ELISA for measuri...

journal_title：Journal of immunological methods

authors： Zouali M,Stollar BD

更新日期：1986-06-10 00:00:00

abstract：:Since macrophages (MPH) are able to adhere firmly to solid surfaces, the recovery of viable and functional MPH has proven to be extremely difficult. We have developed a simple method using agarose coating for preparing MPH and culturing the cells in suspension. Their properties were tested over 72 h. The oxidative bur...

journal_title：Journal of immunological methods

authors： Château MT,Rabesandratana H,Caravano R

更新日期：1991-09-20 00:00:00

abstract：:We have developed specific immunoassays for secretory peroxidase (SP) and for myeloperoxidase (MP) (polymorphonuclear leukocyte-derived peroxidase) in human saliva. Antibodies against SP and MP were produced using bovine milk lactoperoxidase (LP) and human MP as the immunogens, respectively. The methods developed are ...

journal_title：Journal of immunological methods

authors： Vilja P,Lumikari M,Tenovuo J,Sievers G,Tuohimaa P

更新日期：1991-08-09 00:00:00

abstract：:A radioimmunoassay (RIA) was developed for the quantitative measurement of a glycoprotein which was purified from human milk-fat-globule membrane (MFGM) and termed MFGM-pg 70. The assay with a sensitivity of detecting 30 ng of MFGM-pg 70/ml was employed to quantitate the levels of MFGM-gp 70 shed in supernatants from ...

journal_title：Journal of immunological methods

authors： Imam A,Neville AM

更新日期：1986-11-20 00:00:00

abstract：:ADP-ribosylation factors (Arf) are small GTP-binding proteins involved in vesicular transport and the activation of phospholipase D (PLD). The conversion of Arf-GDP to Arf-GTP is promoted in vivo by guanine nucleotide exchange factors such as ARNO or cytohesin-1. In order to examine the expression of ARNO and cytohesi...

journal_title：Journal of immunological methods

authors： Garceau V,Houle MG,Chouinard F,Gagnon S,Harbour D,Naccache PH,Bourgoin SG

更新日期：2001-03-01 00:00:00

abstract：:In the present work we demonstrate the efficient display of functional scFv antibodies on the bacteriophage lambda capsid. A single-chain (scFv) anti-CEA antibody gene was cloned in two different vectors to obtain fusion of the scFv antibody to the N- or C-terminus of the bacteriophage lambda capsid protein D (gpD). L...

journal_title：Journal of immunological methods

authors： Vaccaro P,Pavoni E,Monteriù G,Andrea P,Felici F,Minenkova O

更新日期：2006-03-20 00:00:00

abstract：:Multiplex assays detecting sets of related clinical analytes simultaneously can save considerable amount of time and resources. Array-in-well (AIW) is a powerful platform for the multiplex detection of different analytes where microarrays can be printed at the bottom of microtiter wells, thus combining the potential o...

journal_title：Journal of immunological methods

authors： Talha SM,Saviranta P,Hattara L,Vuorinen T,Hytönen J,Khanna N,Pettersson K

更新日期：2016-02-01 00:00:00

abstract：:Mold contamination and exposure to fungi in indoor environments has been associated with various adverse health effects but little is known about the significance of individual fungal species in the initiation or exacerbation of such effects. Using Stachybotrys chartarum as a model fungus we sought to demonstrate that...

journal_title：Journal of immunological methods

authors： Schmechel D,Simpson JP,Beezhold D,Lewis DM

更新日期：2006-02-20 00:00:00

abstract：:A simple method is described for the preparation of vimentin-type intermediate filaments for solid-phase immunoassays. Bovine aortic endothelial cells were grown to confluency in multiwells, and extracted with Triton X-100 and a high-salt buffer. Residual protein in the wells consisted of 53% vimentin as judged by SDS...

journal_title：Journal of immunological methods

authors： Hansson GK

更新日期：1985-12-27 00:00:00

abstract：:Multivalent antibody fragments (scFv dimers, trimers and tetramers) provide high avidity and ideal pharmacokinetics for tumour targeting applications. This protocol describes our optimised protocol for high-level bacterial synthesis of soluble antibody scFv fragments, as either monomers or multimers, using the heat-in...

journal_title：Journal of immunological methods

authors： Power BE,Hudson PJ

更新日期：2000-08-28 00:00:00

abstract：:Antibody phage display technology is a well established method for selecting specific antibodies against desired targets. Although phage display is the most widely used method of generating synthetic antibodies, it is laborious to perform multiple selections with different antigens simultaneously using conventional ma...

journal_title：Journal of immunological methods

authors： Kiss MM,Babineau EG,Bonatsakis M,Buhr DL,Maksymiuk GM,Wang D,Alderman D,Gelperin DM,Weiner MP

更新日期：2011-03-31 00:00:00

abstract：:The open reading frame of the α-subunit (amino acids 1-211) of human muscle nicotinic acetylcholine receptor (hAChR(α211)) was inserted into eukaryotic expression vector of pPIC9K to form a recombinant plasmid. After transformation and expression, the target protein rhAChR(α211) (recombinant hAChR(α211)) was secretive...

journal_title：Journal of immunological methods

authors： Niu L,Guo C,Hao Z,Yuan J,Li Z

更新日期：2011-09-30 00:00:00

abstract：:We have discovered that 0.5-0.8 M ammonium sulfate has a beneficial effect on the peptic digestion of several antibodies. Initially, the project was begun to address the troublesome precipitation observed when pepsin was used to digest monoclonal antibody CYT-368 (C46), an IgG2a that binds carcinoembryonic antigen. Hi...

journal_title：Journal of immunological methods

authors： Rea DW,Ultee ME

更新日期：1993-01-04 00:00:00

abstract：:A method is described for immunizing mice with a protein-cellulose complex obtained by covalent coupling of antigenic protein molecules to suspended cellulose particles. Primary immunization of the animals with the complex led to a pronounced immune response persisting for 20-30 days. Subsequent administration of the ...

journal_title：Journal of immunological methods

authors： Gurvich AE,Korukova A

更新日期：1986-03-13 00:00:00

abstract：:The human beta2-microglobulin antigen-antibody system was used as a model to illustrate the versatility of a microradioimmunoassay technique using protein A-bearing Staphylococcus aureus Cowan I strain (SACI) bacteria as a non-specific immunoadsorbent in place of a second antibody. Experimental conditions are describe...

journal_title：Journal of immunological methods

authors： Natali PG,Pellegrino MA,Walker L,Ferrone S,Reisfeld RA

更新日期：1979-01-01 00:00:00

abstract：:The purpose of this study was to develop a flow cytometric method for the detection of adenosine deaminase (ADA) in a single cell suspension of mononuclear cells. Anti-human ADA antibody was purified by affinity chromatography on a column of Sepharose 4B to which calf ADA was covalently linked. This antibody was used ...

journal_title：Journal of immunological methods

authors： SenGupta S,Petsche D,Gelfand EW,Chechik BE

更新日期：1985-06-25 00:00:00

abstract：:Over the past 10 years an immunoperoxidase method using dinitrophenyl (DNP) hapten-labelled primary or secondary probes has been devised. Its widely successful application in research and diagnostic work has depended upon the development of certain key reagents. These include a novel non-deleterious DNP labelling comp...

journal_title：Journal of immunological methods

authors： Jasani B,Thomas ND,Navabi H,Millar DM,Newman GR,Gee J,Williams ED

更新日期：1992-06-24 00:00:00

abstract：:When limited amounts of antigens are available, it is often difficult to obtain large quantities of polyclonal antisera. Although antisera can be raised in small rodents, yields are usually small. To circumvent these problems, we have designed a modified method for generating polyclonal ascites fluid (AF). Using the a...

journal_title：Journal of immunological methods

authors： Ou SK,Hwang JM,Patterson PH

更新日期：1993-09-27 00:00:00

abstract：:Recombinant fusion proteins, consisting of a monovalent anti-human RBC monoclonal antibody B6, and conserved immunodominant peptide of HIV-1 envelope glycoprotein gp41 or HIV-2 envelope glycoprotein gp36, have been designed and purified after over-expression in E. coli. These fusion proteins are Fab-based and were obt...

journal_title：Journal of immunological methods

authors： Gupta A,Gupta S,Chaudhary VK

更新日期：2001-10-01 00:00:00

abstract：:Immune analysis of polypeptides separated by SDS-PAGE provides a technique useful in the characterisation of complex mixtures. We describe a simple procedure involving immune analysis of fractionated PAGE gels. Polypeptides of purified herpesvirus simplex, HSV 'excreted antigen' and cell culture 'control' preparations...

journal_title：Journal of immunological methods

authors： Cleator GM,Klapper PE,Cropper LM,Coupes D,Longson M

更新日期：1984-03-16 00:00:00

abstract：:A C1q solid phase microassay was designed for the rapid detection of circulating immune complexes. Its level of sensitivity is comparable to that of the Raji cell and greater than the C1q binding assay; furthermore, it is faster and low in cost. These conditions make it more practical and applicable in the clinical se...

journal_title：Journal of immunological methods

authors： Orozco A,Contreras CE,Sánchez P,Meilijson O,Bianco NE

更新日期：1983-04-29 00:00:00

abstract：:The differences between murine and human neutrophils mean that findings in mice may not translate to humans, and therefore an in vivo model with human neutrophils would be an important methodological advance. We generated humanised mice by injecting human cord blood derived CD34+ stem cells into irradiated NOD-scid-γc...

journal_title：Journal of immunological methods

authors： Coughlan AM,Freeley SJ,Robson MG

更新日期：2012-11-30 00:00:00

abstract：:Murine lymphocytes readily undergo spontaneous and glucocortocoid-induced apoptosis in vitro. It has been previously demonstrated that during apoptosis, many cell types including lymphocytes, enzymatically cleave their DNA, thus demonstrating a sub-G0 DNA peak when stained with propidium iodide and analyzed by flow cy...

journal_title：Journal of immunological methods

authors： Douglas RS,Tarshis AD,Pletcher CH Jr,Nowell PC,Moore JS

更新日期：1995-12-27 00:00:00

abstract：:A novel enzyme-linked bio-immunoassay (bio-ELISA) has been developed to detect interferon-gamma (IFN-gamma) induced HLA-DR antigen on the surface of human tumor cells. Cells are cultured at 37 degrees C in 96-well microtiter plates in the presence of IFN-gamma for 2 days. After fixation with reagent alcohol the HLA-DR...

journal_title：Journal of immunological methods

authors： Gibson UE,Kramer SM

更新日期：1989-12-20 00:00:00

abstract：:The histamine content of commercial preparations of pokeweed mitogen was measured by amino acid analysis technique, automated fluorometry, and bioassay employing the guinea pig ileum. Ten samples from 5 companies were examined and found to contain between 0.026 micrograms and 167.5 micrograms of histamine per ml of so...

journal_title：Journal of immunological methods

authors： Seltzer JM,O'Connor RD,Simon RA,Hugli TE

更新日期：1982-12-30 00:00:00

abstract：:An important factor limiting better understanding of the protective role of sIgA at mucosal surfaces is the limited availability of the purified immunoglobulin. Among other things, purified sIgA is needed for use as a standard in measurements of the concentration of this immunoglobulin in mucosal secretions, particula...

journal_title：Journal of immunological methods

authors： Parr EL,Bozzola JJ,Parr MB

更新日期：1995-03-27 00:00:00

abstract：:Overlapping peptide scans prepared by Spot synthesis have been used to map interaction sites in several systems. Here we report our experience with this approach to identify peptides from the variable parts of anti-hapten, anti-peptide and anti-protein antibodies that retain their specific antigen-binding capacity in ...

journal_title：Journal of immunological methods

authors： Laune D,Molina F,Ferrières G,Villard S,Bès C,Rieunier F,Chardès T,Granier C

更新日期：2002-09-01 00:00:00

abstract：:A high non-specific binding of immunoglobulins to plastic surfaces was noted with a number of rat sera, when tested in an indirect 125I-labeled protein-A assay for detection of cell-surface-bound rat immunoglobulins of various classes and IgG subclasses. This type of non-specific binding was found with all types of Ig...

journal_title：Journal of immunological methods

authors： Nilsson R,Sjögren HO

更新日期：1984-01-20 00:00:00

abstract：:Application of antibody phage display to the identification of cell surface antigens with restricted expression patterns is often complicated by the inability to demonstrate specific binding to a certain cell type. The specificity of an antibody can only be properly assessed when the antibody is of sufficient high aff...

journal_title：Journal of immunological methods

authors： Geuijen CA,Clijsters-van der Horst M,Cox F,Rood PM,Throsby M,Jongeneelen MA,Backus HH,van Deventer E,Kruisbeek AM,Goudsmit J,de Kruif J

更新日期：2005-07-01 00:00:00