Abstract:
:5'-Bromodeoxyuridine (BrdU) present in the course of late S and G2 phases of the cell cycle in PHA-stimulated human lymphocyte cultures causes the despiralization and elongation of some chromosome regions, including short arms of acrocentric chromosomes. BrdU present at a concentration of 250 microM during the last 10 h in lymphocyte cultures from 19 healthy subjects did not affect the number of silver-stained NORs, but raised significantly the number of satellite associations of acrocentric chromosomes. The mere substitution of thymine by BrdU in DNA strands as a reason for increased number of satellite associations seems a less plausible explanation than the modification of DNA-protein complexes of NOR regions, which could alter the degree of their spiralization and cause the increased tendency of acrocentric chromosomes to associate in the subsequent metaphase.
journal_name
Hum Genetjournal_title
Human geneticsauthors
Musilová J,Michalová K,Hoffmanová Hdoi
10.1007/BF00286640subject
Has Abstractpub_date
1983-01-01 00:00:00pages
91-3issue
2eissn
0340-6717issn
1432-1203journal_volume
65pub_type
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