Abstract：
:Hematopoiesis in continuous bone marrow culture is dependent upon close-range interaction between hematopoietic cells and marrow-derived adherent cells. The latter have been shown to include a significant proportion of fibroblastic elements, synthesizing the attachment protein fibronectin. The role of fibronectin in continuous bone marrow culture was therefore investigated. Continuous marrow cultures were stained by the peroxidase-anti-peroxidase technique, using an affinity-purified anti-fibronectin antibody. The stained cultures were then examined by transmission electron microscopy for the presence of fibronectin at sites of cellular attachment. Fibronectin was detected on the substratum attachment surfaces of marrow-derived adherent cells and at sites of interaction between stromal elements in the adherent layer. Fibronectin was also detected at many attachment sites between marrow-derived adherent cells and developing granulocytes and monocytes. The possible significance of these observations is discussed in relation to the hematopoietic microenvironment.
journal_name
Exp Hematoljournal_title
Experimental hematologyauthors
Bentley SA,Tralka TSsubject
Has Abstractpub_date
1983-02-01 00:00:00pages
129-38issue
2eissn
0301-472Xissn
1873-2399journal_volume
11pub_type
杂志文章abstract：:Bone marrow stem cells reside in close proximity to endosteal osteoblasts. To explore the potential role of osteoblasts in hematopoietic differentiation, we measured the mRNA accumulation, protein production, and secretion of hematopoietic growth factors by the nonmineralizing MG-63 and the mineralizing SaOS-2 human o...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
abstract：OBJECTIVE:Peripheral blood progenitor cells (PBPC) have been established as an alternative source of hematopoietic stem cells for allogeneic transplantation, but an increased incidence of both acute and chronic graft-vs-host disease (GVHD) has become apparent. We performed a prospective randomized trial comparing bone ...
journal_title：Experimental hematology
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0301-472x(03)00195-4
更新日期：2003-10-01 00:00:00
abstract：:The relative contributions of various organs to platelet production is controversial. In this study, serial histologic sections of bone marrow, spleen, liver, and lung from normal C57BL/6J mice and mice that had received three different agents which perturb normal murine thrombopoiesis (platelet antiserum, 5-fluoroura...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:An increasing amount of data provides strong evidence for the complex multifactorial control of primary hemopoietic functions. Here we present a new multicellular functional unit, the Hematon, isolated from the light-density floating fraction of normal human bone marrow (BM) aspirates. The Hematon is organized in a co...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：:The kinetics of cell turnover for myeloid/monocyte cells that form colonies in agar (GM-CFC) were measured through the progressive increase in their sensitivity to 313-nm light during a period of cell labeling with BrdCyd. Two components of cell killing with distinctly separate labeling kinetics revealed both the pres...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：OBJECTIVES:We previously demonstrated that allogeneic bone marrow transplantation (BMT) after low-dose total lymphoid irradiation (200 cGy) and depletion of donor-reactive cells with cyclophosphamide (Cy) converted recipients to graft-vs-host disease (GVHD)-free chimeras tolerant to donor skin grafts. BMT also generate...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/s0301-472x(02)01009-3
更新日期：2003-01-01 00:00:00
abstract：OBJECTIVE:Preservation of hematopoietic stem and progenitor cells from early radiation-induced apoptosis is the rationale for emergency antiapoptotic cytokine therapy (EACK) after radiation accidents. This strategy is based on the combination of stem cell factor + Flt3-ligand + thrombopoietin + interleukin 3 (SFT3). Th...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2007.04.017
更新日期：2007-08-01 00:00:00
abstract：:The advent of single-cell transcriptomics has led to the proposal of a number of novel high-resolution models for the hematopoietic system. Testing the predictions generated by such models requires cell fate potential assays of matching, single-cell resolution. Here we detail the development of an in vitro high-throug...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2018.01.005
更新日期：2018-04-01 00:00:00
abstract：OBJECTIVE:Unresponsiveness to chemotherapy is a major problem in the treatment of leukemia, which can be caused by unresponsiveness of noncycling cells to cell cycle-dependent cytotoxic agents. Targeted toxins consisting of a targeting and activating cytokine (granulocyte-macrophage colony-stimulating factor [GM-CSF]) ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2003.11.005
更新日期：2004-02-01 00:00:00
abstract：OBJECTIVE:All-trans retinoic acid (ATRA) has been used as the first-line therapy for patients with acute promyelocytic leukemia (APL). We previously reported that ATRA increased serum thrombopoietin (TPO) levels accompanied by thrombocytosis during ATRA therapy for APL. In this study, we investigated the mechanism of t...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2003.10.009
更新日期：2004-01-01 00:00:00
abstract：:The anemia of Belgrade b/b rats has been shown to be due to intracellular iron deficiency. The aim of this study of erythropoiesis at the progenitor cell level in these rats was to determine if a defect is present in the early phase of red cell production. Both erythroid colony-forming unit (CFU-E)- and erythroid burs...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
abstract：:The reproducibility and utility of an in vitro bioassay for erythropoietin were evaluated, and modifications, including the use of frozen target cells, were made. The assay, based on 3H-thymidine incorporation into phenylhydrazine-treated mouse spleen cells, showed a dose-response curve from 0.2 to 20 mU of sheep plas...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1987-05-01 00:00:00
abstract：:Murine low-density bone marrow cells sorted from the blast cell window on the basis of high rhodamine-123 retention (Rh-bright), are highly enriched in histamine-, IL-4-, and IL-6-producing cells. We established by in situ hybridization that up to 50% of this population (around 0.25% of the whole bone marrow) coexpres...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/s0301-472x(99)00039-9
更新日期：1999-06-01 00:00:00
abstract：:Improving our understanding of the intricacies of hematopoietic specification of induced or embryonic human pluripotent stem cells is beneficial for many areas of research and translational medicine. Currently, it is not clear whether, during human pluripotent stem cells hematopoietic differentiation in vitro, the mat...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2018.05.007
更新日期：2018-09-01 00:00:00
abstract：:HIV-derived lentiviral vectors have been used widely to transduce non-dividing cells, such as hematopoietic stem cells (HSCs), in the setting of gene therapy. In this study, we screened lentiviral vectors for their ability to drive expression of the murine MHC class II chaperone, invariant chain (Ii) and a GFP reporte...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2013.07.002
更新日期：2013-11-01 00:00:00
abstract：:An unusual case of graft failure following bone marrow transplantation for aplastic anemia is reported in which the patient had delayed and incomplete recovery of hematopoiesis despite documentation of sustained engraftment. The pathophysiology of graft failure following bone marrow transplantation is discussed and re...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:To investigate the value of genetic mutations in the pathogenesis and differential diagnosis of hemophagocytic lymphohistiocytosis (HLH), mutations related to blood and immune deficiency genes were analyzed in patients with HLH. Peripheral blood samples from 33 children diagnosed with HLH on the basis of the 2004 diag...
journal_title：Experimental hematology
pub_type： 临床试验,杂志文章
doi：10.1016/j.exphem.2019.09.024
更新日期：2019-10-01 00:00:00
abstract：:Four types of colonies can be formed by progenitor cells (CFUc) in double layer agar cultures: eosinophil, granulocyte, macrophage, and mixed granulocyte-macrophage. We have developed a method for in situ staining of intact agar plates with luxol fast blue and acetoorcein employed to differentiate the type of colonies...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1980-11-01 00:00:00
abstract：:Thrombocytopenia is an important clinical problem for many acquired immunodeficiency syndrome (AIDS) patients. Recently, the utility of recombinant cytokines in alleviating the hematopoietic complications of AIDS and AIDS therapy has been evaluated. The newly cloned cytokine stem cell factor (SCF) has been demonstrate...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:The use of neonatal instead of fetal calf serum, as a constituent of the culture medium, confronted the authors with the problem of toxicity in the fetal liver cell erythropoietin assay. Tested human sera were toxic at rather low concentrations. Heating the human sera for 30 min at 56 degrees C abolished the toxicity....
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：:A simple two-step procedure has been developed for the production of human urinary erythropoietin suitable for tissue culture use. Lyophilized human urinary protein was fractionated by phenyl-Sepharose chromatography, the erythropoietin binding in 2 M lithium chloride and being eluted by an ascending linear gradient t...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1985-09-01 00:00:00
abstract：:Serum obtained from (C3H X C57BL) F1 hybrid mice injected with parental (C57BL) lymph node cells after 600 rad irradiation contained a high level of an erythropoietic factor, which enhanced erythroid colony formation by fetal C57BL liver cells in methylcellulose cultures. The serum level of this erythroid colony-stimu...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:Our previous studies have shown that a unique glycoprotease from Pasteurella haemolytica specifically cleaves only proteins containing sialylated O-linked glycans. The hematopoietic progenitor cell antigen, CD34, which is heavily glycosylated with both N- and O-linked glycans, is readily cleaved by this protease. In t...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1992-06-01 00:00:00
abstract：BACKGROUND:In patients treated with rituximab and alemtuzumab for lymphomas or CLL, antibody-dependent cellular cytotoxicity (ADCC) is a major mechanism of action. Therefore, assessment of ADCC is mandatory to understand the complex mechanisms leading to the anti-lymphoma effects of monoclonal antibodies (mAb). Due to ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2006.02.015
更新日期：2006-06-01 00:00:00
abstract：:The circadian and circannual rhythms of committed stem cells (CFUc) were investigated by using the agar colony technique. There was a marked circadian rhythmicity, with variations between maximum and minimum colony yields of 2-5.5 times; such variations could also be seen throughout the year. A phase shift was observe...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：:In the accompanying paper we constructed a model for the proliferation and differentiation of granulocyte-macrophage progenitor cells in response to the specific proliferation/differentiation stimulus granulocyte-macrophage colony-stimulating activity (gm-CSA). Here we have tested the model without altering the model ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：
更新日期：1985-02-01 00:00:00
abstract：OBJECTIVE:Our previous studies have demonstrated differentially expressed genetic signature patterns in adult peripheral blood (APB) monocytes (Mos) vs cord blood (CB) Mos. MATERIALS AND METHODS:In this study, we examined the differential gene expression profiles of APB vs CB immature dendritic cells (iDCs) and mature...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2009.07.010
更新日期：2009-10-01 00:00:00
abstract：:The aberrant overexpression of Wilms tumor-1 (WT1) in acute myeloid leukemia (AML) plays an important role in blast cell survival by enhancing proliferation and inhibiting apoptosis. However, the mechanism underlying the overexpression of WT1 remains unclear. Here, we identified miR-193a (miR-193a-5p) and miR-600 targ...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2018.02.001
更新日期：2018-05-01 00:00:00
abstract：:We analyzed the accessory function of malignant B cells from non-Hodgkin's lymphomas (NHLs). Among the 70 samples of malignant B cells included, four patterns of expression of the costimulatory molecules CD80 and CD86 were distinguished (+/+, +/-, -/+ and -/-). In two-thirds of the cases, CD80, CD86, or both were expr...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/s0301-472x(98)00059-9
更新日期：1999-03-01 00:00:00
abstract：:Anamorsin (AM) is an antiapoptotic molecule that confers factor-independent survival on hematopoietic cells. AM-deficient (AM(-/-)) mice are embryonic lethal because of a defect in definitive hematopoiesis; however, the significance of AM in embryonic hematopoiesis remains unknown. This study characterized the hematop...
journal_title：Experimental hematology
pub_type： 杂志文章
doi：10.1016/j.exphem.2014.01.002
更新日期：2014-05-01 00:00:00
