Abstract:
:A sixty base pair DNA duplex containing the nucleotide sequence of the bacteriophage T5 early (T5P25) promoter has been constructed using a combination of chemical synthesis and enzymatic methods. Subsequent to cloning into pBR322, the promoter has been demonstrated to be biologically active being capable of directing the efficient expression of genes under its control. This serves as a prototype for an approach to the study of the in vivo structure-function relationships and efficiency of promoters.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Rommens J,MacKnight D,Pomeroy-Cloney L,Jay Edoi
10.1093/nar/11.17.5921subject
Has Abstractpub_date
1983-09-10 00:00:00pages
5921-40issue
17eissn
0305-1048issn
1362-4962journal_volume
11pub_type
杂志文章abstract::Random amplified polymorphic DNA (RAPD) markers are used widely to develop high resolution genetic maps and for genome fingerprinting. Typically, single oligomers of approximately 10 nucleotides are used to PCR amplify characteristic RAPD marker fragments. We describe an efficient method for the direct end-sequencing ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.19.e28
更新日期:1999-10-01 00:00:00
abstract::Members of the basic helix-loop-helix (bHLH) family of transcription factors regulate a wide array of developmental processes in many cell types, including cell fate specification, differentiation and morphogenesis. Our studies describe the cloning of a gene from the nematode Caenorhabditis elegans that is closely rel...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.21.4423
更新日期:2001-11-01 00:00:00
abstract::N-(guanosin-8-yl) acetylaminofluorene (Guo-AAF) was prepared by the reaction of N-acetoxy-N-2-acetylaminofluorene (AAAF) and guanosine. Antibodies to Guo-AAF were elicited in rabbits by immunization with bovine serum albumin-Guo-AAF conjugate. The antibodies were purified by affinity chromatography on a Sepharose-Guo-...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.2.733
更新日期:1979-02-01 00:00:00
abstract::The c-myc proto-oncogene encodes a highly unstable mRNA. Stabilized, truncated myc transcripts have been found in several human and murine tumors of hematopoietic origin. Recently, two tumors expressing 3' truncated c-myc mRNAs that were five times more stable than normal myc transcripts, were described. We have tried...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.9.2387
更新日期:1991-05-11 00:00:00
abstract::Studying the intricate folding of rRNA within the ribosome remains a complex problem. Phenanthroline-Cu(II) complexes cleave phosphodiester bonds in rRNA in specific regions, apparently especially where the rRNA structure is constrained in some fashion. We have introduced phenanthroline-copper complexes into 50S Esche...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.8.1906
更新日期:1999-04-15 00:00:00
abstract::Simian cells have been transformed with SV40 origin-defective recombinant plasmids containing the tsA209 T-antigen gene. These plasmids contain deletions of either 5 or 52 nucleotides that include the BglI site at the SV40 ori, are defective for replication in COS-1 cells but retain a functional SV40 early promoter. T...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.22.7913
更新日期:1985-11-25 00:00:00
abstract::RegAnalyst is a user-friendly web interface that integrates MoPP (Motif Prediction Program), MyPatternFinder (pattern detection tool) and MycoRegDB (mycobacterial promoter and regulatory elements database). Since motif discovery is a challenging task, numerous tools have been developed over the past few years. Strikin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp388
更新日期:2009-07-01 00:00:00
abstract::We have examined the formation of alternate strand triple-helices at the target sequence A11(TC)6.(GA)6T11 using the oligonucleotides T11(AG)6 and T11(TG)6, by DNase I footprinting. These third strands were designed so as to form parallel T.AT triplets together with antiparallel G.GC and A.AT or T.AT triplets. We find...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.19.3977
更新日期:1994-09-25 00:00:00
abstract::Aspects of the metabolism of RNA have been studied in enzymatically isolated protoplasts from cotyledon and first leaf mesophyll tissue of two cultivars of cucumber. The first leaf mesophyll protoplasts incorporated (3H)-uridine into ribosomal RNA at a constant rate for up to 25 hr in a simple salts medium and for up ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/2.7.1111
更新日期:1975-07-01 00:00:00
abstract::Myotonic dystrophy type 1 (DM1) is an autosomal dominant multisystemic disorder caused by expansion of CTG triplet repeats in 3'-untranslated region of DMPK gene. The pathomechanism of DM1 is driven by accumulation of toxic transcripts containing expanded CUG repeats (CUG(exp)) in nuclear foci which sequester several ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv163
更新日期:2015-03-31 00:00:00
abstract::Our web server, PIZSA (http://cospi.iiserpune.ac.in/pizsa), assesses the likelihood of protein-protein interactions by assigning a Z Score computed from interface residue contacts. Our score takes into account the optimal number of atoms that mediate the interaction between pairs of residues and whether these contacts...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz368
更新日期:2019-07-02 00:00:00
abstract::The secondary structures of the 5'-untranslated region (5'-UTR) of five different tymoviruses have been determined by structure probing, computer prediction and sequence comparison. Despite large sequence differences, there are remarkable similarities in the secondary structure. In all viruses two or four hairpins are...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.24.4910
更新日期:1996-12-15 00:00:00
abstract::A mutant at the carboxyl end of the terminal protein, p3, of phage phi 29 DNA has been constructed by inserting an containing the stop translation codon TGA in the three possible reading frames, immediately downstream of a phage phi 29 DNA fragment coding for all but the last five amino acids of protein p3. The activ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.21.7397
更新日期:1983-11-11 00:00:00
abstract::Lateral ribosomal stalk is responsible for binding and recruiting translation factors during protein synthesis. The eukaryotic stalk consists of one P0 protein with two copies of P1•P2 heterodimers to form a P0(P1•P2)₂ pentameric P-complex. Here, we have solved the structure of full-length P1•P2 by nuclear magnetic re...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt636
更新日期:2013-10-01 00:00:00
abstract::Unfolding of the nucleosomes in transcriptionally active chromatin uncovers the sulfhydryl groups of histone H3 and permits the selective recovery of the unfolded nucleosomes by mercury-affinity chromatography. This new technique has been used to compare the nucleosomal proteins and their postsynthetic modifications i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.11.4375
更新日期:1987-06-11 00:00:00
abstract::Understanding the evolutionary and genomic mechanisms responsible for turning the soil-derived saprophytic mycobacteria into lethal intracellular pathogens is a critical step towards the development of strategies for the control of mycobacterial diseases. In this context, Mycobacterium indicus pranii (MIP) is of speci...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks793
更新日期:2012-11-01 00:00:00
abstract::Specific targeting of protein–nucleic acid interactions is an area of current interest, for example, in the regulation of gene-expression. Most transcription factor proteins bind in the DNA major groove; however, we are interested in an approach using small molecules to target the minor groove to control expression by...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1232
更新日期:2017-02-17 00:00:00
abstract::The targeted adduction of aflatoxin B1- exo -8,9-epoxide (AFB1- exo -8,9-epoxide) to a specific guanine within an oligodeoxyribonucleotide containing multiple guanines was achieved using a DNA triplex to control sequence selectivity. The oligodeoxyribonucleotide d(AGAGAAGATTTTCTTCTCTTTTTTTTCTCTT), designated '3G', spo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.4.1070
更新日期:1998-02-15 00:00:00
abstract::Here we advocate the use of 2-dimensional data representation in the context of the informational approach of sequence analysis (Claverie & Bougueleret (1986) Nucleic Acids Research 14, 179-196) by applying these methods to the problem of intron/exon discrimination. Two main findings are reported: i) oligonucleotide p...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.5.1729
更新日期:1988-03-11 00:00:00
abstract::The synthetic DNA fragment (formula, see text) (corresponding to nucleotides 4299-4314 of the phi X DNA sequence) was cloned into either the AmpR gene or the KmR gene of plasmid pACYC 177. The DNA sequence of the KmR gene around the insertion site was determined by nucleotide sequence analysis of the pACYC 177 FnudII ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.14.3335
更新日期:1981-07-24 00:00:00
abstract::The sigma(54) factor associates with core RNA polymerase (RNAP) to form a holoenzyme that is unable to initiate transcription unless acted on by an activator protein. sigma(54) is closely involved in many steps of activator-dependent transcription, such as core RNAP binding, promoter recognition, activator interaction...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp419
更新日期:2009-07-01 00:00:00
abstract::Procollagen alpha 2 (I) mRNA can be induced congruent to 6-fold in primary avian tendon (PAT) cells on addition of ascorbate to the culture medium. Previously, we have shown that the induction is linear after a 12 h lag and requires a total of 60-72 h to achieve maximum levels. We have now investigated in more detail ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/12.5.2569
更新日期:1984-03-12 00:00:00
abstract::The pattern of sites for cleavage mediated by topoisomerase II was determined in 830 kb of cloned DNA from the Drosophila X chromosome, with the objectives of comparing it with mapped structural and functional landmarks and examining if the correlations with such landmarks reported in individual loci can be generalize...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.11.2041
更新日期:1997-06-01 00:00:00
abstract::Tomato golden mosaic geminivirus has a genome of two single-stranded (ss) DNA components, A and B. An almost identical 'common' region in DNA A and DNA B is thought to contain sequence elements controlling replication and transcription. Hence investigation of sequences important for DNA replication by in vitro mutagen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.24.10213
更新日期:1989-12-25 00:00:00
abstract::Recently, due to the dual roles of DNA and enzyme, DNAzyme has been widely used in the field of DNA circuit, which has a wide range of applications in bio-engineered system, information processing and biocomputing. In fact, the activity of DNAzymes was regulated by subunits assembly, pH control and metal ions triggers...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky1245
更新日期:2019-02-20 00:00:00
abstract::Many types of DNA structures are generated in response to DNA damage, repair and recombination that require processing via specialized nucleases. DNA hairpins represent one such class of structures formed during V(D)J recombination, palindrome extrusion, DNA transposition and some types of double-strand breaks. Here w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1059
更新日期:2012-03-01 00:00:00
abstract::Rad18 is a ubiquitin E3 ligase that monoubiquitinates PCNA on stalled replications forks. This allows recruitment of damage-tolerant polymerases for damage bypass and DNA repair. In this activity, the Rad18 protein has to interact with Rad6, the E2 ubiquitin-conjugating enzyme, ubiquitin, PCNA and DNA. Here we analyze...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm615
更新日期:2007-01-01 00:00:00
abstract::LocDB is a manually curated database with experimental annotations for the subcellular localizations of proteins in Homo sapiens (HS, human) and Arabidopsis thaliana (AT, thale cress). Currently, it contains entries for 19,604 UniProt proteins (HS: 13,342; AT: 6262). Each database entry contains the experimentally der...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq927
更新日期:2011-01-01 00:00:00
abstract::A cDNA encoding the 60 kDa subunit of mammalian splicing factor SF3a has been isolated. The deduced protein sequence reveals a 30% identity to the PRP9 splicing protein of the yeast S.cerevisiae. The highest homology is present in a zinc finger-like region in the C-terminal domain of both proteins. The PRP9 zinc finge...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.24.5223
更新日期:1994-12-11 00:00:00
abstract::The alternatively spliced RNA species of tumor suppressor gene p53, containing an additional 96 bases derived from intron 10, is present at approximately 25 to 30% the level of regularly spliced p53 RNA in both normal epidermal and carcinoma cells. The presence of this alternatively spliced RNA in 10T1/2 fibroblast ce...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.8.1979
更新日期:1992-04-25 00:00:00