Abstract:
:Poly 2'0-ethylcytidylic acid (poly (Ce)) was prepared by polymerization of 2'-0-ethylcytidine-5'-pyrophosphate with Escherichia coli polynucleotide phosphorylase in the presence of Mn++, and its properties compared with those of poly (rC), poly (Cm) and poly (dC). The neutral form of pOLY (Ce) exhibits properties similar to those of poly (rC) and poly (Cm). It also forms an acid twin-stranded helix with a transition pH of 5.9 in 0.1 M NaCl. The neutral form readily forms a double-stranded helical complex with poly (rI). Relative to poly (Cm), replacement of the 2'-0-methyl by 2-0-ethyl leads to increased enhancement of the thermal stabilities of both the acid helical form of poly (Ce) and its complex with poly (rI).
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Kielanowska M,Shugar Ddoi
10.1093/nar/3.3.817subject
Has Abstractpub_date
1976-03-01 00:00:00pages
817-24issue
3eissn
0305-1048issn
1362-4962journal_volume
3pub_type
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