Abstract:
:Post-transcriptional protein synthesis by GH3 cloned pituitary cells, which secrete prolactin and growth hormone, is dependent on Ca2+. The effects of antagonists of prolactin secretion were examined on overall protein synthesis in GH3 cells as a function of cellular Ca2+ depletion and restoration at varying concentrations of extracellular Ca2+. Leucine incorporation by Ca2+-depleted cells during short incubations was reduced by 80-90%. Trifluoperazine at micromolar concentrations inhibited leucine incorporation in a Ca2+-dependent manner. The extent of inhibition varied with extracellular Ca2+ concentration and was fully reversed at higher Ca2+ concentrations. Similar patterns of inhibition of leucine incorporation were observed with nifedipine, verapamil, calmidazolium, chlorpromazine, bromocriptine, ergotamine, and the (+)- and (-)-isomers of butaclamol, but dopamine, apomorphine, and chlorpromazine sulfoxide were not inhibitory. Muscarinic agonists decreased incorporation in a Ca2+-dependent manner, but lesser degrees of inhibition were obtained. Inhibitions were observed for a broad spectrum of polypeptide species, could not be explained by effects on Mg2+ availability or amino acid uptake, and were rapidly and fully reversed by Ca2+. Production of prolactin and growth hormone was decreased by secretory inhibitors to the same extent and with the same Ca2+ concentration dependence as was observed for inhibition of amino acid incorporation. It is proposed that these substances inhibit protein synthesis in GH3 cells through alterations in intracellular Ca2+ metabolism rather than through mechanisms mediated by calmodulin or dopamine receptors.
journal_name
Mol Pharmacoljournal_title
Molecular pharmacologyauthors
Wolfe SE,Brostrom CO,Brostrom MAsubject
Has Abstractpub_date
1986-04-01 00:00:00pages
411-9issue
4eissn
0026-895Xissn
1521-0111journal_volume
29pub_type
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journal_title:Molecular pharmacology
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pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
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更新日期:1984-07-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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更新日期:1982-11-01 00:00:00
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journal_title:Molecular pharmacology
pub_type: 杂志文章
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更新日期:1994-08-01 00:00:00
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