Abstract：
:The two forms of pituitary adenylate cyclase-activating polypeptide, PACAP27 and PACAP38, are two neuropeptide hormones related to the vasoactive intestinal peptide/secretin/ glucagon family of peptides. PACAP receptors that are positively coupled to adenylyl cyclase and phospholipase C have been identified in cultured cerebellar granule cells. Using the reverse transcription-polymerase chain reaction methodology, we demonstrated the expression of the PACAP-R and PACAP-R-hop mRNAs in cultured granule cells. When grown in the absence of serum or in low K+ concentrations, these neurons underwent apoptosis, a naturally occurring process characterized by cell shrinkage and internucleosomal DNA cleavage. We used these models of programmed cell death to study the relationship between PACAP receptor activation and neuronal apoptosis. Treatment with PACAP27 and PACAP38 reduced the development of apoptosis in a dose-dependent manner. The neuroprotective activity of PACAP was mimicked by high concentrations of vasoactive intestinal peptide or forskolin but not by carbamylcholine. Thus, we suggest that the activation of type I PACAP receptors may contribute to the survival of cerebellar granule neurons.
journal_name
Mol Pharmacoljournal_title
Molecular pharmacologyauthors
Cavallaro S,Copani A,D'Agata V,Musco S,Petralia S,Ventra C,Stivala F,Travali S,Canonico PLsubject
Has Abstractpub_date
1996-07-01 00:00:00pages
60-6issue
1eissn
0026-895Xissn
1521-0111journal_volume
50pub_type
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更新日期：1987-12-01 00:00:00
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更新日期：1989-06-01 00:00:00
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pub_type： 评论,杂志文章,评审
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更新日期：2006-02-01 00:00:00
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更新日期：1998-06-01 00:00:00
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更新日期：1990-11-01 00:00:00
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doi：
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doi：
更新日期：1992-03-01 00:00:00
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