Abstract：
:Phenylalanyl-tRNA synthetase (EC 6.1.1.20) from the extreme thermophile Thermus thermophilus HB8 has been isolated and crystallized. The enzyme was found to consist of two types of subunits with molecular masses 38 X 10(3) (alpha) and 94 X 10(3) (beta) and is likely to be a tetrameric protein with a molecular mass of about 260 X 10(3) (alpha 2 beta 2). Crystals of phenylalanyl-tRNA synthetase were grown by the hanging-drop technique at 4 degrees C in the presence of ammonium sulfate. Trigonal crystals, space group P3(1)21, with cell dimensions a = b = 176 A and c = 142 A (1 A = 0.1 nm), are suitable for medium-resolution X-ray analysis.
journal_name
J Mol Bioljournal_title
Journal of molecular biologyauthors
Chernaya MM,Korolev SV,Reshetnikova LS,Safro MGdoi
10.1016/0022-2836(87)90301-9subject
Has Abstractpub_date
1987-12-05 00:00:00pages
555-6issue
3eissn
0022-2836issn
1089-8638pii
0022-2836(87)90301-9journal_volume
198pub_type
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