Abstract:
Background:Fibroblast growth factor 2 (FGF2) is a highly pleiotropic cytokine with antifibrotic activity in wound healing. During the process of wound healing and fibrosis, fibroblasts are the key players. Although accumulating evidence has suggested the antagonistic effects of FGF2 in the activation process of fibroblasts, the mechanisms by which FGF2 hinders the fibroblast activation remains incompletely understood. This study aimed to identify the key genes and their regulatory networks in skin fibroblasts treated with FGF2. Methods:RNA-seq was performed to identify the differentially expressed mRNA (DEGs) and lncRNA between FGF2-treated fibroblasts and control. DEGs were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Furthermore, the networks between mRNAs and lncRNAs were constructed by Pearson correlation analysis and the networkanalyst website. Finally, hub genes were validated by real time-PCR. Results:Between FGF2-treated fibroblasts and control fibroblasts, a total of 1475 DEGs was obtained. These DEGs were mainly enriched in functions such as the ECM organization, cell adhesion, and cell migration. They were mainly involved in ECM-receptor interaction, PI3K-Akt signaling, and the Hippo pathway. The hub DEGs included COL3A1, COL4A1, LOX, PDGFA, TGFBI, and ITGA10. Subsequent real-time PCR, as well as bioinformatics analysis, consistently demonstrated that the expression of ITGA10 was significantly upregulated while the other five DEGs (COL3A1, COL4A1, LOX, PDGFA, TGFBI) were downregulated in FGF2-treated fibroblasts. Meanwhile, 213 differentially expressed lncRNAs were identified and three key lncRNAs (HOXA-AS2, H19, and SNHG8) were highlighted in FGF2-treated fibroblasts. Conclusion:The current study comprehensively analyzed the FGF2-responsive transcriptional profile and provided candidate mechanisms that may account for FGF2-mediated wound healing.
journal_name
PeerJjournal_title
PeerJauthors
Wu B,Tang X,Zhou Z,Ke H,Tang S,Ke Rdoi
10.7717/peerj.10671subject
Has Abstractpub_date
2021-01-15 00:00:00pages
e10671issn
2167-8359pii
10671journal_volume
9pub_type
杂志文章相关文献
PeerJ文献大全abstract::We often expect that investigations of the patterns, causes, and consequences of among-individual variation in a trait of interest will reveal how selective pressures or ecological conditions influence that trait. However, many endocrine traits, such as concentrations of glucocorticoid (GC) hormones, exhibit adaptive ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.4398
更新日期:2018-02-21 00:00:00
abstract::The genus Elatine contains ca 25 species, all of which are small, herbaceous annuals distributed in ephemeral waters on both hemispheres. However, due to a high degree of morphological variability (as a consequence of their amphibious life-style), the taxonomy of this genus remains controversial. Thus, to fill this ga...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2800
更新日期:2016-12-21 00:00:00
abstract::Background. In recent years, there has been an explosion in the number of technical and medical diagnostic platforms being developed. This has greatly improved our ability to more accurately, and more comprehensively, explore and characterize human biological systems on the individual level. Large quantities of biomed...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.177
更新日期:2013-10-03 00:00:00
abstract:Background:The highest rate of cancer-related deaths worldwide is from lung adenocarcinoma (LUAD) annually. Metabolism was associated with tumorigenesis and cancer development. Metabolic-related genes may be important biomarkers and metabolic therapeutic targets for LUAD. Materials and Methods:In this study, the glean...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.10008
更新日期:2020-09-24 00:00:00
abstract::[This corrects the article DOI: 10.7717/peerj.4110.]. ...
journal_title:PeerJ
pub_type: 杂志文章,已发布勘误
doi:10.7717/peerj.4110/correction-1
更新日期:2017-12-20 00:00:00
abstract:Background:Plant reproduction is influenced by the net outcome of plant-herbivore and plant-pollinator interactions. While both herbivore impacts and pollinator impacts on plant reproduction have been widely studied, few studies examine them in concert. Methodology:Here, we review the contemporary literature that exam...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.9049
更新日期:2020-06-08 00:00:00
abstract::Grazing and litter removal can alter understory structure and composition after afforestation, posing a serious threat to sustainable forest development. Enclosure is considered to be an effective measure to restore degraded forest restoration. However, little is known about the dynamics of soil nutrients and microbia...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.8857
更新日期:2020-03-25 00:00:00
abstract::Seasonal upwelling at the northern Pacific coast of Costa Rica offers the opportunity to investigate the effects of pronounced changes in key water parameters on fine-scale dynamics of local coral reef communities. This study monitored benthic community composition at Matapalo reef (10.539°N, 85.766°W) by weekly obser...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1434
更新日期:2015-11-24 00:00:00
abstract::We investigated population dynamics, breeding pairs, breeding habitat selection, nest density, distance between neighboring nests, nest survival, reproductive success, and recruitment rate for Black-necked Cranes (BNC, Grus nigricollis) during 2013-2015 in Yanchiwan National Nature Reserve (YCW), Gansu, China. Numbers...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2939
更新日期:2017-01-31 00:00:00
abstract::Inflammation is critical in the dysregulated growth of adipose tissue and associated vascular dysfunctions. 15-Lipoxygenase metabolites, important mediators of inflammation in adipose tissue during obese conditions, may contribute to codependence of inflammation and angiogenesis in adipose tissue. We have already repo...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.635
更新日期:2014-10-21 00:00:00
abstract::Outbreaks of the processionary moth Thaumetopoea pityocampa (Denis & Schiffermüller, 1775), a forest pest from the Palearctic, are thought to induce a behavioral response of bats, but up to now the moth has been seldom identified as bats' prey. Studies on bat diets suggest moths with cyclical outbreaks attract a wide ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7169
更新日期:2019-07-05 00:00:00
abstract::The variation between the actual and perceived lightness of a stimulus has strong dependency on its background, a phenomena commonly known as lightness induction in the literature of visual neuroscience and psychology. For instance, a gray patch may perceptually appear to be darker in a background while it looks brigh...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.5626
更新日期:2018-09-25 00:00:00
abstract::Cell immobilization is an alternative to microencapsulation for the maintenance of cells in a liquid medium. However, artificial immobilization carriers are expensive and pose a high safety risk. Okara, a food-grade byproduct from soymilk production, is rich in prebiotics. Lactobacilli could provide health enhancing e...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2701
更新日期:2016-11-10 00:00:00
abstract::It can be challenging to identify scleractinian corals from the genus Pocillopora Lamarck 1816 in the field because of their large range of inter- and intra-specific morphological variation that co-occur with changes in the physical environment. This task is made more arduous in the context of a depth gradient, where ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.5797
更新日期:2018-10-25 00:00:00
abstract::Recent upperward migration of plants and animals along altitudinal gradients and poleward movement of animal range boundaries have been confirmed by many studies. This phenomenon is considered to be part of the fingerprint of recent climate change on the biosphere. Here I examine whether poleward movement is occurring...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.77
更新日期:2013-05-28 00:00:00
abstract::In humans and apes, yawn contagion echoes emotional contagion, the basal layer of empathy. Hence, yawn contagion is a unique tool to compare empathy across species. If humans are the most empathic animal species, they should show the highest empathic response also at the level of emotional contagion. We gathered data ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.519
更新日期:2014-08-12 00:00:00
abstract:Background:Weight management interventions involving behaviour change often utilise face to face interventions which include evidence based behaviour change strategies yet are costly and time intensive. In contrast, digital interventions cost less and have a wider reach yet tend to lack an evidence base and are less ef...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6907
更新日期:2019-05-15 00:00:00
abstract::Garden Warblers Sylvia borin were studied during autumn stopover in Crete before crossing the barrier of the Mediterranean Sea and the Sahara Desert. Birds followed with transmitters show extensive stopover periods, which were longer in first-year birds, 16 days, compared with adult birds, 14 days. The distribution of...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.319
更新日期:2014-03-25 00:00:00
abstract:INTRODUCTION:There is much literature devoted to the problem of drug resistance and decreased susceptibility of fungi to commonly used antifungals. AIM:To analyze drug susceptibility of Candida albicans and non-Candida albicans strains isolated from the hands of people without any symptoms of disease over a 16-year pe...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3038
更新日期:2017-02-23 00:00:00
abstract::Wildlife demography is typically studied at a single point in time within a year when species, often during the reproductive season, are more active and therefore easier to find. However, this provides only a low-resolution glimpse into demographic temporal patterns over time and may hamper a more complete understandi...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.8658
更新日期:2020-02-25 00:00:00
abstract::As conspicuous modular components of benthic marine habitats, gorgonian (sea fan) octocorals have perplexed taxonomists for centuries through their shear diversity, particularly throughout the Indo-Pacific. Phenotypic incongruence within and between seemingly unitary lineages across contrasting environments can provid...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1128
更新日期:2015-08-20 00:00:00
abstract::Metabolic dysfunction in skeletal muscle is a major contributor to the development of type 2 diabetes. Endurance exercise training has long been established as an effective means to directly restore skeletal muscle glucose and lipid uptake and metabolism. However, in addition to the direct effects of skeletal muscle o...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.605
更新日期:2014-09-30 00:00:00
abstract::Urmia Lake, the largest natural habitat of the brine shrimp Artemia urmiana, has progressively desiccated over the last two decades, resulting in a loss of 80% of its surface area and producing thousands of hectares of arid salty land. This ecological crisis has seriously affected the lake's native biodiversity. Artem...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7190
更新日期:2019-07-02 00:00:00
abstract:BACKGROUND:In Burkina Faso, it is not uncommon for mothers to drink alcohol, even during pregnancy. We aimed to study the association between maternal alcohol consumption during pregnancy and the child's cognitive performance using the Kaufman Assessment Battery for Children, 2nd edition (KABC-II) and the Children's Ca...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3507
更新日期:2017-06-30 00:00:00
abstract::Rao's quadratic diversity index is one of the most widely applied diversity indices in functional and phylogenetic ecology. The standard way of computing Rao's quadratic diversity index for an ecological assemblage with a group of species with varying abundances is to sum the functional or phylogenetic distances betwe...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.5211
更新日期:2018-07-06 00:00:00
abstract::Background. Genome-wide association studies have succeeded in detecting novel common variants which associate with complex diseases. As a result of the fast changes in next generation sequencing technology, a large number of sequencing data are generated, which offers great opportunities to identify rare variants that...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2139
更新日期:2016-06-16 00:00:00
abstract:Background:Many fish taxa produce sound in voluntary and in disturbance contexts but information on the full acoustic repertoire is lacking for most species. Yet, this knowledge is critical to enable monitoring fish populations in nature through acoustic monitoring. Methods:In this study we characterized the sounds em...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.8559
更新日期:2020-02-24 00:00:00
abstract::Deep-sea hydrothermal vents in the western Pacific are increasingly being assessed for their potential mineral wealth. To anticipate the potential impacts on biodiversity and connectivity among populations at these vents, environmental baselines need to be established. Bathymodiolus manusensis is a deep-sea mussel fou...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3655
更新日期:2017-08-21 00:00:00
abstract::A range of diseases is associated with amyloid fibril formation. Despite different proteins being responsible for each disease, all of them share similar features including beta-sheet-rich secondary structure and fibril-like protein aggregates. A number of proteins can form amyloid-like fibrils in vitro, resembling st...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1271
更新日期:2015-09-24 00:00:00
abstract:Objective:To explore the blood pressure response to different ablation points of renal denervation (RDN) in patients with resistant hypertension. Methods:A total of 42 cases with resistant hypertension treated by RDN in our center from 2013 to 2015 were retrospectively analyzed. The patients were divided into two grou...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.9842
更新日期:2020-09-15 00:00:00