Abstract：
:Together with their undeniable role in the ecology of arid and semiarid ecosystems, Agave species are emerging as a model to dissect the relationships between crassulacean acid metabolism and high efficiency of light and water use, and as an energy crop for bioethanol production. Transcriptome resources from economically valuable Agaves species, such as Agave tequilana and A. salmiana, as well as hybrids for fibers, are now available, and multiple gene expression landscape analyses have been reported. Key components in molecular mechanisms underlying drought tolerance could be uncovered by analyzing gene expression patterns of roots. This study describes an efficient protocol for high-quality total RNA isolation from phenolic compounds-rich Agave roots. Our methodology involves suitable root handling and collecting in the field and using saving-time commercial kits available. RNA isolated from roots free of lignified out-layers and clean cortex showed high values of quality and integrity according to electrophoresis and microfluidics-based platform. Synthesis of long full-length cDNAs and PCR amplification tested the suitability for downstream applications of extracted RNA. The protocol was applied successfully to A. tequilana roots but can be used for other Agave species that also develop lignified epidermis/exodermis in roots.
journal_name
3 Biotechjournal_title
3 Biotechauthors
Maceda-López LF,Villalpando-Aguilar JL,García-Hernández E,Ávila de Dios E,Andrade-Canto SB,Morán-Velázquez DC,Rodríguez-López L,Hernández-Díaz D,Chablé-Vega MA,Trejo L,Góngora-Castillo E,López-Rosas I,Simpson J,Alatorre-Cobos Fdoi
10.1007/s13205-020-02620-8subject
Has Abstractpub_date
2021-02-01 00:00:00pages
75issue
2eissn
2190-572Xissn
2190-5738pii
2620journal_volume
11pub_type
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