Ionic requirements for the specific binding of [3H]GBR 12783 to a site associated with the dopamine uptake carrier.


:At 0 degrees C, when Na+ was the only cation present in the incubation medium, increasing the Na+ concentration from 3 to 10 mM enhanced the affinity of [3H]1-[2-(++di-phenylmethoxy)ethyl]-4-(3-phenyl-2-propenyl)piperaz ine [( 3H]GBR 12783) for the specific binding site present in rat striatal membranes without affecting the Bmax. For higher Na+ concentrations, specific binding values plateaued and then slightly decreased at 130 mM Na+. In a 10 mM Na+ medium, the KD and the Bmax were, respectively, 0.23 nM and 12.9 pmol/mg of protein. In the presence of 0.4 nM [3H]GBR 12783, the half-maximal specific binding occurred at 5 mM Na+. A similar Na+ dependence was observed at 20 degrees C. Scatchard plots indicated that K+, Ca2+, Mg2+, and Tris+ acted like competitive inhibitors of the specific binding of [3H]GBR 12783. The inhibitory potency of various cations (K+, Ca2+, Mg2+, Tris+, Li+, and choline) was enhanced when the Na+ concentration was decreased from 130 to 10 mM. In a 10 mM Na+ medium, the rank order of inhibitory potency was Ca2+ (0.13 mM) greater than Mg2+ greater than Tris+ greater than K+ (15 mM). The requirement for Na+ was rather specific, because none of the other cations acted as a substitute for Na+. No anionic requirement was found: Cl-, Br-, and F- were equipotent. These results suggest that low Na+ concentrations are required for maximal binding; higher Na+ concentrations protect the specific binding site against the inhibitory effect of other cations.


J Neurochem


Bonnet JJ,Benmansour S,Vaugeois JM,Costentin J




Has Abstract


1988-03-01 00:00:00












  • Substance P hydrolysis by human serum cholinesterase.

    abstract::Highly purified human serum cholinesterase (EC, also known as pseudocholinesterase and butyrylcholinesterase) had peptidase activity toward substance P. Digestion of substance P was monitored by high performance liquid chromatography, which separated three product peptides. The cleavages occurred sequentially....

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lockridge O

    更新日期:1982-07-01 00:00:00

  • An allosteric modulator of metabotropic glutamate receptors (mGluR₂), (+)-TFMPIP, inhibits restraint stress-induced phasic glutamate release in rat prefrontal cortex.

    abstract::The potential anxiolytic effects of a novel positive allosteric modulator (PAM) of the metabotropic glutamate receptor subgroup 2 (mGluR₂) were investigated using a self-referencing recording technique with enzyme-based microelectrode arrays (MEAs) that reliably measures tonic and phasic changes in extracellular gluta...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hascup ER,Hascup KN,Pomerleau F,Huettl P,Hajos-Korcsok E,Kehr J,Gerhardt GA

    更新日期:2012-08-01 00:00:00

  • Activation of cholecystokinin neurons in the dorsal pallium of the telencephalon is indispensable for the acquisition of chick imprinting behavior.

    abstract::Chick imprinting behavior is a good model for the study of learning and memory. Imprinting object is recognized and processed in the visual wulst, and the memory is stored in the intermediate medial mesopallium in the dorsal pallium of the telencephalon. We identified chicken cholecystokinin (CCK)-expressing cells loc...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Maekawa F,Nakamori T,Uchimura M,Fujiwara K,Yada T,Tsukahara S,Kanamatsu T,Tanaka K,Ohki-Hamazaki H

    更新日期:2007-09-01 00:00:00

  • Thalamic synaptic transmission of sensory information modulated by synergistic interaction of adenosine and serotonin.

    abstract::The thalamic synapses relay peripheral sensory information to the cortex, and constitute an important part of the thalamocortical network that generates oscillatory activities responsible for different vigilance (sleep and wakefulness) states. However, the modulation of thalamic synaptic transmission by potential slee...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Yang YC,Hu CC,Huang CS,Chou PY

    更新日期:2014-03-01 00:00:00

  • Enhancing the GLP-1 receptor signaling pathway leads to proliferation and neuroprotection in human neuroblastoma cells.

    abstract::Increasing evidence suggests that glucagon-like peptide-1 (GLP-1), an incretin hormone of current interest in type 2 diabetes, is neuroprotective in both cell culture and animal models. To characterize the neuroprotective properties of GLP-1 and associated underlying mechanisms, we over-expressed the GLP-1 receptor (G...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Li Y,Tweedie D,Mattson MP,Holloway HW,Greig NH

    更新日期:2010-06-01 00:00:00

  • Competitive inhibition of N-acetylated-alpha-linked acidic dipeptidase activity by N-acetyl-L-aspartyl-beta-linked L-glutamate.

    abstract::The endogenous neuropeptide N-acetyl-L-aspartyl-L-glutamate (NAAG) fulfills several criteria required to be accepted as a neurotransmitter. NAAG inactivation may proceed through enzymatic hydrolysis into N-acetyl-L-aspartate and glutamate by an N-acetylated-alpha-linked acidic dipeptidase (NAALADase). Therefore, some ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Serval V,Barbeito L,Pittaluga A,Cheramy A,Lavielle S,Glowinski J

    更新日期:1990-07-01 00:00:00

  • Reduction in glial fibrillary acidic protein mRNA abundance induced by (-)-deprenyl and other monoamine oxidase B inhibitors in C6 glioma cells.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Li XM,Qi J,Juorio AV,Boulton AA

    更新日期:1993-10-01 00:00:00

  • Depletion of neuronal endoplasmic reticulum calcium stores by thapsigargin: effect on protein synthesis.

    abstract::We have used thapsigargin (TG), a specific, irreversible inhibitor of endoplasmic reticulum (ER) Ca(2+)-ATPases, and caffeine, an agonist of the ryanodine receptor, to study the effect of emptying of ER calcium stores on protein synthesis in neuronal cells. TG at 1 microM caused a permanent inhibition of protein synth...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Paschen W,Doutheil J,Gissel C,Treiman M

    更新日期:1996-10-01 00:00:00

  • Hydralazine inhibits compression and acrolein-mediated injuries in ex vivo spinal cord.

    abstract::We have previously shown that acrolein, a lipid peroxidation byproduct, is significantly increased following spinal cord injury in vivo, and that exposure to neuronal cells results in oxidative stress, mitochondrial dysfunction, increased membrane permeability, impaired axonal conductivity, and eventually cell death. ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hamann K,Nehrt G,Ouyang H,Duerstock B,Shi R

    更新日期:2008-02-01 00:00:00

  • Annexins in the human neuroblastoma SH-SY5Y: demonstration of relocation of annexins II and V to membranes in response to elevation of intracellular calcium by membrane depolarisation and by the calcium ionophore A23187.

    abstract::The human neuroblastoma SH-SY5Y was found to express annexins I, II, IV, V, and VI by western blot analysis. Calcium-dependent membrane-binding proteins were isolated from SH-SY5Y and analysed by 2-dimensional gel electrophoresis. Proteins with Mr and Pi values similar to those of annexins I, II, III, IV, V, and VI we...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Blanchard S,Barwise JL,Gerke V,Goodall A,Vaughan PF,Walker JH

    更新日期:1996-08-01 00:00:00

  • Rapid phosphorylation of phospholipase C gamma 1 by brain-derived neurotrophic factor and neurotrophin-3 in cultures of embryonic rat cortical neurons.

    abstract::Phospholipase C gamma 1 (PLC-gamma 1) is involved at an early step in signal transduction of many hormones and growth factors and catalyzes the hydrolysis of phosphatidylinositol (PI) 4,5-bisphosphate to diacylglycerol and inositol trisphosphate, two potent intracellular second messenger molecules. The transformation ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Widmer HR,Kaplan DR,Rabin SJ,Beck KD,Hefti F,Knüsel B

    更新日期:1993-06-01 00:00:00

  • Dimercaprol is an acrolein scavenger that mitigates acrolein-mediated PC-12 cells toxicity and reduces acrolein in rat following spinal cord injury.

    abstract::Acrolein is one of the most toxic byproducts of lipid peroxidation, and it has been shown to be associated with multiple pathological processes in trauma and diseases, including spinal cord injury, multiple sclerosis, and Alzheimer's disease. Therefore, suppressing acrolein using acrolein scavengers has been suggested...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Tian R,Shi R

    更新日期:2017-06-01 00:00:00

  • Pretreatment of cerebellar granule cells with concanavalin A potentiates quisqualate-stimulated phosphoinositide hydrolysis.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Raulli R,Danysz W,Wroblewski JT

    更新日期:1991-06-01 00:00:00

  • Selective linkage of mitochondrial enzymes to intracellular calcium stores differs between human-induced pluripotent stem cells, neural stem cells, and neurons.

    abstract::Mitochondria and releasable endoplasmic reticulum (ER) calcium modulate neuronal calcium signaling, and both change in Alzheimer's disease (AD). The releasable calcium stores in the ER are exaggerated in fibroblasts from AD patients and in multiple models of AD. The activity of the alpha-ketoglutarate dehydrogenase co...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Chen H,Cross AC,Thakkar A,Xu H,Li A,Paull D,Noggle SA,Kruger L,Denton TT,Gibson GE

    更新日期:2020-08-31 00:00:00

  • Dose-dependent, K+-stimulated efflux of endogenous taurine from primary astrocyte cultures is Ca2+-dependent.

    abstract::The K+-stimulated efflux of endogenous taurine from primary rat cerebellar astrocyte cultures prepared from 7-9-day-old rats was studied at 16-18 days in vitro using HPLC analysis. Taurine efflux was dose-dependent at K+ concentrations between 10 mM and 80 mM, with an EC50 of approximately 50 mM. Maximum stimulation o...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Philibert RA,Rogers KL,Allen AJ,Dutton GR

    更新日期:1988-07-01 00:00:00

  • Multiple sites of ethanol action in alpha1 and alpha2 glycine receptors suggested by sensitivity to pressure antagonism.

    abstract::The current study used an ethanol antagonist, increased atmospheric pressure, to test the hypothesis that ethanol acts on multiple sites in glycine receptors (GlyRs). The effects of 12 times normal atmospheric pressure of helium-oxygen gas (pressure) on ethanol-induced potentiation of GlyR function in Xenopus oocytes ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Davies DL,Crawford DK,Trudell JR,Mihic SJ,Alkana RL

    更新日期:2004-06-01 00:00:00

  • Glutamate inhibits adenylate cyclase activity in dispersed rat hippocampal cells directly via an N-methyl-D-aspartate-like metabotropic receptor.

    abstract::Three major subtypes of glutamate receptors that are coupled to cation channels--N-methyl-D-aspartate (NMDA), kainate, and alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptors--are known as ionotropic receptors in the mammalian CNS. Recently, an additional subtype that is coupled to GTP binding protei...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Itano Y,Murayama T,Kitamura Y,Nomura Y

    更新日期:1992-09-01 00:00:00

  • Molecular and functional characterization of an Na+-independent choline transporter in rat astrocytes.

    abstract::In this study, we examined the molecular and functional characterization of choline uptake into cultured rat cortical astrocytes. Choline uptake into astrocytes showed little dependence on extracellular Na+. Na+-independent choline uptake was saturable and mediated by a single transport system, with an apparent Michae...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Inazu M,Takeda H,Matsumiya T

    更新日期:2005-09-01 00:00:00

  • Developmental changes in the differential expression of two serotonin 5-HT3 receptor splice variants in the rat.

    abstract::PCR was used to isolate identical partial cDNA clones encoding a serotonin 5-HT3 receptor subunit from rat nodose and superior cervical ganglia. The amino acid sequence predicted from these clones, extending from the putative transmembrane domain I to the stop codon, demonstrated a 93% homology with the 5-HT3 receptor...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Miquel MC,Emerit MB,Gingrich JA,Nosjean A,Hamon M,el Mestikawy S

    更新日期:1995-08-01 00:00:00

  • G proteins coupled to phospholipase C: molecular targets of long-term ethanol exposure.

    abstract::Long-term ethanol exposure is known to inhibit bradykinin-stimulated phosphoinositide hydrolysis in cultures of neuroblastoma x glioma 108-15 cells. In the present study, [3H]bradykinin binding, GTP-binding protein function, and phospholipase C activity were assayed in cells grown for 4 days in 100 mM ethanol with the...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Simonsson P,Rodriguez FD,Loman N,Alling C

    更新日期:1991-06-01 00:00:00

  • Direct and continuous detection of ATP secretion from primary monolayer cultures of bovine adrenal chromaffin cells.

    abstract::A method was developed for direct and continuous detection of secretion of ATP from primary monolayer cultures of bovine adrenal chromaffin cells. ATP, which is costored with catecholamines within adrenal chromaffin cells, was released into the incubation medium, where it reacted with firefly luciferin-luciferase prod...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: White TD,Bourke JE,Livett BG

    更新日期:1987-10-01 00:00:00

  • The dipeptidyl peptidase IV inhibitor vildagliptin suppresses development of neuropathy in diabetic rodents: effects on peripheral sensory nerve function, structure and molecular changes.

    abstract::Incretin-related therapy was found to be beneficial for experimental diabetic neuropathy, but its mechanism is obscure. The purpose of this study is to explore the mechanism through which dipeptidyl peptidase IV inhibitor, vildagliptin (VG), influences neuropathy in diabetic rodents. To this end, non-obese type 2 diab...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Tsuboi K,Mizukami H,Inaba W,Baba M,Yagihashi S

    更新日期:2016-02-01 00:00:00

  • Cyclosporin A, an immunosuppressive drug, induces programmed cell death in rat C6 glioma cells by a mechanism that involves the AP-1 transcription factor.

    abstract::Cyclosporin A (CsA) is a clinically important immunosuppressive drug widely used to prevent graft rejection following organ or bone marrow transplantation. Although there are reports of serious neurologic alterations associated with the use of the drug, the precise mechanism of its action on the CNS still remains unkn...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Mosieniak G,Figiel I,Kaminska B

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  • P-glycoprotein in blood-brain barrier endothelial cells: interaction and oligomerization with caveolins.

    abstract::P-glycoprotein (P-gp), an adenosine triphosphate (ATP)-binding cassette transporter which acts as a drug efflux pump, is highly expressed at the blood-brain barrier (BBB) where it plays an important role in brain protection. Recently, P-gp has been reported to be located in the caveolae of multidrug-resistant cells. I...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Jodoin J,Demeule M,Fenart L,Cecchelli R,Farmer S,Linton KJ,Higgins CF,Béliveau R

    更新日期:2003-11-01 00:00:00

  • DARPP-32 in the orchestration of responses to positive natural stimuli.

    abstract::Dopamine- and cAMP-regulated phosphoprotein (Mr 32 kDa, DARPP-32) is an integrator of multiple neuronal signals and plays a crucial role particularly in mediating the dopaminergic component of the systems involved in the evaluation of stimuli and the ensuing elaboration of complex behavioral responses (e.g., responses...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Scheggi S,De Montis MG,Gambarana C

    更新日期:2018-11-01 00:00:00

  • Neuropeptide Y in frontal cortex is not altered in major depression.

    abstract::Previously, we reported a modest but significant reduction in the concentration of neuropeptide Y in frontal cortices from victims of suicide relative to age-matched natural or accidental death control subjects. The reduction in neuropeptide Y appeared to be greatest in a subgroup of victims of suicide for which there...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ordway GA,Stockmeier CA,Meltzer HY,Overholser JC,Jaconetta S,Widdowson PS

    更新日期:1995-10-01 00:00:00

  • Excitatory and inhibitory effects of A1 and A2A adenosine receptor activation on the electrically evoked [3H]acetylcholine release from different areas of the rat hippocampus.

    abstract::The modulation by adenosine analogues and endogenous adenosine of the electrically evoked release of [3H]acetylcholine ([3H]ACh) was compared in subslices of the three areas of the rat hippocampus (CA1, CA3, and dentate gyrus). The mixed A1/A2 agonist 2-chloroadenosine (CADO; 2-10 microM) inhibited, in a concentration...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Cunha RA,Milusheva E,Vizi ES,Ribeiro JA,Sebastião AM

    更新日期:1994-07-01 00:00:00

  • Phosphorylation and transcriptional activity regulation of retinoid-related orphan receptor alpha 1 by protein kinases C.

    abstract::Retinoid-related orphan receptor alpha1 (RORalpha1) is a member of the nuclear receptor superfamily. It is highly expressed in CNS particularly in the cerebellum. Absence of this transcription factor in mice leads to several abnormalities, such as cerebellar atrophy linked to Purkinje cell death and impaired different...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Duplus E,Gras C,Soubeyre V,Vodjdani G,Lemaigre-Dubreuil Y,Brugg B

    更新日期:2008-03-01 00:00:00

  • Translational regulation of GluR2 mRNAs in rat hippocampus by alternative 3' untranslated regions.

    abstract::The glutamate receptor 2 (GluR2) subunit determines many of the functional properties of the alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate subtype of glutamate receptor. The roles of untranslated regions (UTRs) in mRNA stability, transport, or translation are increasingly recognized. The 3' end of the GluR2 tra...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Irier HA,Shaw R,Lau A,Feng Y,Dingledine R

    更新日期:2009-04-01 00:00:00

  • Synaptosomal calcium metabolism during hypoxia and 3,4-diaminopyridine treatment.

    abstract::A decline in the calcium-dependent release of neurotransmitters appears to underlie the decreased neuronal function that accompanies reduced oxygen tensions (hypoxia). To determine if alterations in calcium uptake are primary to these changes, synaptosomal calcium uptake was measured in the presence of 100%, 2.5%, or ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Peterson C,Gibson GE

    更新日期:1984-01-01 00:00:00