Abstract：
:We have previously reported that insulin/insulin-like growth factor (IGF)-I induced the alpha 1 isoform of Na+,K(+)-ATPase in cultured astrocytes. In this study the effects of insulin/IGF-I on Na+,K(+)-ATPase activity and cell proliferation were examined in astrocytes cultured under the various conditions, to test the possible involvement of the enzyme activity in the mitogenic action of IGF-I on astrocytes. Insulin increased Na+,K(+)-ATPase activity and stimulated cell proliferation in subconfluent astrocytes (cultured for 7-14 days in vitro). In contrast, these effects were not observed in confluent cells (cultured for 28 days). Furthermore, insulin stimulated neither the enzyme activity nor [3H]thymidine incorporation in astrocytes preincubated in fetal calf serum-free medium for 2 days (quiescent cells) and treated with dibutyryl cyclic AMP (differentiated cells). The increases in Na+,K(+)-ATPase activity and expression of the alpha 1 mRNA preceded the mitogenic effect. 125I-IGF-I binding experiment showed that all the cells used here had similar binding characteristics. The insulin-induced increase in enzyme activity was not affected by 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7), and it was observed even in Ca(2+)-free medium. The stimulation by IGF-I of [3H]thymidine incorporation was attenuated by ouabain and a low external K+ level. These findings suggest that stimulation of Na+,K(+)-ATPase activity is involved in the mitogenic action of IGF-I on cultured astrocytes.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Matsuda T,Murata Y,Tanaka K,Hosoi R,Hayashi M,Tamada K,Takuma K,Baba Adoi
10.1046/j.1471-4159.1996.66020511.xsubject
Has Abstractpub_date
1996-02-01 00:00:00pages
511-6issue
2eissn
0022-3042issn
1471-4159journal_volume
66pub_type
杂志文章abstract：:Although the causes of psychiatric disorders are not fully understood, it is well established that mental illness originates from the interaction between genetic and environmental factors. In this regard, compelling evidence demonstrates that depression can be the consequence of altered, and often maladaptive, respons...
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doi：10.1111/j.1471-4159.1980.tb09000.x
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journal_title：Journal of neurochemistry
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doi：10.1111/j.1471-4159.1991.tb08168.x
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pub_type： 杂志文章,评审
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：1981-08-01 00:00:00
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journal_title：Journal of neurochemistry
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doi：10.1111/j.1471-4159.1981.tb00462.x
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journal_title：Journal of neurochemistry
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更新日期：1996-05-01 00:00:00
