Abstract:
Background and Aims:Schistosomiasis japonica is a widespread human zoonotic disease, and in China, there are many patients with schistosomiasis suffering from liver fibrosis. Many studies have shown that natural killer (NK) cells could reduce the progression of hepatic fibrosis by directly killing hepatic stellate cells (HSCs). However, NK cells could not inhibit the progress of liver fibrosis induced by Schistosoma japonicum infection. We aimed to investigate the function of NK cells in schistosomiasis. Methods:BALB/c mice were infected with S. japonicum cercariae. The receptors and their proportions expressed on NK cells in the liver and spleen from infected mice were detected using flow cytometry. Levels of IFN-γ, perforin, and granzyme of NK cells, and collagen I, III, and α-SMA of hepatic tissue, were detected using quantitative real-time PCR. Changes in cytokine levels in sera were detected using a cytometric bead array. Liver fibrosis was evaluated using hematoxylin and eosin and Masson staining. NK function in the schistosomiasis model was analyzed. Results:From 2 to 4 weeks post-infection, NK cells were activated, with significantly increased levels of effector molecules (IFN-γ, perforin, and granzyme) that peaked at 4 weeks after infection. The proportion of NK cells increased in the liver and spleen from 6 to 10 weeks post-infection. However, the function of NK cells was inhibited from 6 to 10 weeks post-infection with significantly decreased levels of activated receptors (AR), inhibitory receptors (IR), and effector molecules. The levels of IFN-γ, IL-12, and IL-6 in mouse serum peaked at 6 weeks post-infection, and IL-10 and IL-21 levels peaked at 8 weeks post-infection. Hepatic fibrosis markers increased significantly at 6 weeks after infection. Conclusion:Our study suggested that NK cells were activated from 2 to 4 weeks post-infection and participated in inflammation in the mouse model. After the S. japonicum laid their eggs, NK cells became inhibited, with decreased levels of both activating and inhibitory NK cell receptors, as well as cytotoxic molecules. In addition, liver fibrosis formed. In mice infected with S. japonicum, the process of liver fibrosis might be alleviated by removing the functional inhibition of NK cells.
journal_name
Front Cell Infect Microbioljournal_title
Frontiers in cellular and infection microbiologyauthors
Hu Y,Wang X,Wei Y,Liu H,Zhang J,Shen Y,Cao Jdoi
10.3389/fcimb.2020.598987subject
Has Abstractpub_date
2020-11-19 00:00:00pages
598987issn
2235-2988journal_volume
10pub_type
杂志文章abstract::Stapylococcus aureus is a common infectious agent in e.g. sepsis, associated with both high mortality rates and severe long-term effects. The cytolytic protein α-hemolysin has repeatedly been shown to enhance the virulence of S. aureus. Combined with an unhindered spread of multi drug-resistant strains, this has trigg...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.571578
更新日期:2020-11-20 00:00:00
abstract::Occurrence of non-Candida albicans Candida (NCAC) species that are associated with elevated MIC values and therapeutic failures are increasing. As a result, timely and accurate means of identification to the species level is becoming an essential part of diagnostic practices in clinical settings. In this study, 301 cl...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00021
更新日期:2019-02-15 00:00:00
abstract::Eradication of Helicobacter pylori has been found to be effective for gastric cancer prevention, but uncertainties remain about the possible adverse consequences such as the potential microbial dysbiosis. In our study, we investigated the association between gut microbiota and H. pylori-related gastric lesions in 47 s...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00202
更新日期:2018-06-19 00:00:00
abstract::Syf1 is a tetratricopeptide repeat (TPR) protein implicated in transcription elongation, spliceosome conformation, mRNA nuclear-cytoplasmic export and transcription-coupled DNA repair. Recently, we identified the spliceosomal components of the human parasite Entamoeba histolytica, among them is EhSyf. Molecular predic...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00229
更新日期:2018-07-09 00:00:00
abstract::Whole genome sequencing (WGS) methods provide new possibilities in the field of molecular epidemiology. This is particularly true for monomorphic organisms where the discriminatory power of traditional methods (e.g., restriction enzyme length polymorphism typing, multi locus sequence typing etc.) is inadequate to eluc...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00089
更新日期:2018-03-22 00:00:00
abstract::Antifungal agents for the treatment of Candida albicans infections are limited. We recently discovered a novel antifungal small molecule, SM21, with promising in vivo activity. Herein, we employed the newly developed C. albicans haploid toolbox to uncover the mechanism of action of SM21. Comprehensive RNA-Seq analyses...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00164
更新日期:2018-06-08 00:00:00
abstract::More than a decade ago a short tandem repeat-based typing method was developed for the fungus Aspergillus fumigatus. This STRAf assay is based on the analysis of nine short tandem repeat markers. Interpretation of this STRAf assay is complicated when there are only one or two differences in tandem repeat markers betwe...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00082
更新日期:2019-03-29 00:00:00
abstract::Cold shock-domain family proteins (Csps) are highly conserved nucleic acid binding proteins regulating the expression of various genes including those involved in stress resistance and virulence in bacteria. We show here that Csps are involved in virulence, cell aggregation and flagella-based extracellular motility of...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00453
更新日期:2017-10-26 00:00:00
abstract::The mechanism by which microRNAs (miRNAs) modulate innate immunity and autophagy has not been fully elucidated in Mycobacterium bovis (M. bovis) infections. In this study, we identified that miR-199a inhibited key innate immune responses and autophagy in murine macrophages infected with M. bovis. Using ex vivo and in ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00238
更新日期:2018-07-10 00:00:00
abstract::Mycobacterium bovis (M. bovis) is the pathogen of animals and humans that can replicate in the phagosomes of myeloid cells. Cytosolic detection of bacterial products plays a crucial role in initiating the innate immune response, including autophagy activation and interferon-β (IFN-β) release. Although IFN-β release an...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00169
更新日期:2017-05-05 00:00:00
abstract::Compounds with novel modes of action are urgently needed to develop effective combination therapies for the treatment of tuberculosis. In this study, a series of compounds was evaluated for activity against replicating Mycobacterium tuberculosis and Vero cell line toxicity. Fourteen of the compounds with in vitro acti...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.582416
更新日期:2020-11-13 00:00:00
abstract::Dermatophytoses account for nearly a quarter of all fungal infections worldwide. These difficult to treat infections of the skin, hair, and nails, are growing more resistant to conventional antifungal treatments, and when treatable, often require prolonged therapeutic regimens. For centuries, essential oils have been ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.545913
更新日期:2020-10-16 00:00:00
abstract::Objectives: To evaluate metagenomic next-generation sequencing (mNGS) as a diagnostic tool in detecting pathogens from osteoarticular infection (OAI) samples. Methods: 130 samples of joint fluid, sonicate fluid, and tissue were prospectively collected from 92 patients with OAI. The performance of mNGS and microbiology...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00471
更新日期:2020-09-17 00:00:00
abstract::Shigella is a Gram-negative bacterium that is responsible for shigellosis. Over the years, the study of Shigella has provided a greater understanding of how the host responds to bacterial infection, and how bacteria have evolved to effectively counter the host defenses. In this review, we provide an update on some of ...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2016.00038
更新日期:2016-03-31 00:00:00
abstract::Pathogenic fungi have developed many strategies to evade the host immune system. Multiple escape mechanisms appear to function together to inhibit attack by the various stages of both the adaptive and the innate immune response. Thus, after entering the host, such pathogens fight to overcome the immune system to allow...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2016.00142
更新日期:2016-11-15 00:00:00
abstract::The oral microbiome can play a role in the instigation and progression of oral diseases that can manifest into other systemic conditions. These associations encourage the exploration of oral dysbiosis leading to the pathogenesis of cancers. In this study, oral rinse was used to characterize the oral microbiome fluctua...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2018.00267
更新日期:2018-08-03 00:00:00
abstract::Persistence has evolved as a potent survival strategy to overcome adverse environmental conditions. This capability is common to almost all bacteria, including all human bacterial pathogens and likely connected to chronic infections caused by some of these pathogens. Although the majority of a bacterial cell populatio...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2020.615450
更新日期:2021-01-14 00:00:00
abstract::Natural killer (NK) cells are critical for targeting and killing tumor, virus-infected and stressed cells as a member of the innate immune system. Recently, NK cells have also emerged as key regulators of adaptive immunity and have become a prominent therapeutic target for cancer immunotherapy and infection control. N...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2020.00231
更新日期:2020-05-19 00:00:00
abstract::The emergence of antimicrobial-resistant (AR) Salmonella has a major concern worldwide. This study was designed to determine the AR profiles and serovars distribution of Salmonella enterica isolated from different breeds of breeder chickens in the province of Henan, China. For this, 2,139 dead embryo samples were coll...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00497
更新日期:2020-09-15 00:00:00
abstract::Clostridium difficile infections (CDI) are a major cause of antibiotic-associated diarrhea. It is hypothesized that CDI develops due to the antibiotic-induced disruption of the intestinal microbial community structure, which allows C. difficile to flourish. Here, we pre-treated weaned pigs with the antibiotics Clindam...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00271
更新日期:2019-08-06 00:00:00
abstract::Influenza is a major public health problem that causes acute respiratory infection in humans. Identification of host factors influencing in disease outcome is critical for recognition of individuals with increased risk. Investigations on the role of rs34481144A and rs12252C IFITM3 polymorphisms in influenza A(H1N1)pdm...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00352
更新日期:2020-07-10 00:00:00
abstract::Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis (TB), which leads to an estimated 1. 5 million deaths worldwide each year. Although the immune correlates of protection against Mtb infection and TB disease have not been well-defined, natural killer (NK) cells are increasingly recognized as a key...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00120
更新日期:2020-03-24 00:00:00
abstract::Gram-positive and Gram-negative pathogens exist as planktonic cells only at limited times during their life cycle. In response to environmental signals such as temperature, pH, osmolality, and nutrient availability, pathogenic bacteria can adopt varied cellular fates, which involves the activation of virulence gene pr...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2019.00421
更新日期:2019-12-11 00:00:00
abstract::Enterococcus faecium is a clinically important pathogen associated with opportunistic infection and multi-drug resistance. E. faecium has been shown to produce membrane vesicles (MVs), but MV production by E. faecium under antibiotic stress conditions and the pathogenic traits thereof have yet to be determined. This s...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00295
更新日期:2019-08-14 00:00:00
abstract::Ten bacteriophages were isolated from faeces and their lytic effects assayed on 103 pathogenic and non-pathogenic Enterobacteriaceae. Two phages (DT1 and DT6) were selected based on their host ranges, and their lytic effects on pathogenic E. coli strains inoculated on pieces of beef were determined. We evaluated the r...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2013.00020
更新日期:2013-06-06 00:00:00
abstract::Apicomplexan parasites, such as human malaria parasites, have complex lifecycles encompassing multiple and diverse environmental niches. Invading, replicating, and escaping from different cell types, along with exploiting each intracellular niche, necessitate large and dynamic changes in parasite morphology and cellul...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2020.611801
更新日期:2021-01-08 00:00:00
abstract::The fungal pathogen Cryptococcus neoformans can cause life-threatening infections in immune compromised individuals. This pathogen is typically acquired via inhalation, and enters the respiratory tract. Innate immune cells such as macrophages and dendritic cells (DCs) are the first host cells that encounter C. neoform...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2020.00037
更新日期:2020-02-11 00:00:00
abstract::Streptococcus pneumoniae is a leading cause of bacterial pneumonia worldwide. Given the critical role of dendritic cells (DCs) in regulating and modulating the immune response to pathogens, we investigated here the role of DCs in S. pneumoniae lung infections. Using a well-established transgenic mouse line which allow...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2013.00021
更新日期:2013-06-21 00:00:00
abstract::The cell surface mucin MUC1 is an important host factor limiting Helicobacter pylori (H. pylori) pathogenesis in both humans and mice by providing a protective barrier and modulating mucosal epithelial and leukocyte responses. The aim of this study was to establish the time-course of molecular events in MUC1-modulated...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00343
更新日期:2020-07-24 00:00:00
abstract::Intestinal microbes are an important system in the human body, with significant effects on behavior. An increasing body of research indicates that intestinal microbes affect brain function and neurogenesis, including sensitivity to stress. To investigate the effects of microbial colonization on behavior, we examined b...
journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00489
更新日期:2017-11-30 00:00:00