Abstract:
:Stapylococcus aureus is a common infectious agent in e.g. sepsis, associated with both high mortality rates and severe long-term effects. The cytolytic protein α-hemolysin has repeatedly been shown to enhance the virulence of S. aureus. Combined with an unhindered spread of multi drug-resistant strains, this has triggered research into novel anti virulence (i.e. anti α-hemolysin) drugs. Their functionality will depend on our ability to identify infections that might be alleviated by such. We therefore saw a need for detection methods that could identify individuals suffering from S. aureus infections where α-hemolysin was a major determinant. Molecular imprinted polymers were subsequently prepared on gold coated sensor chips. Used in combination with a surface plasmon resonance biosensor, α-hemolysin could therethrough be quantified from septic blood samples (n = 9), without pre-culturing of the infectious agent. The biosensor recognized α-hemolysin with high affinity (KD = 2.75 x 10-7 M) and demonstrated a statistically significant difference (p < 0.0001) between the α-hemolysin response and potential sample contaminants. The detection scheme proved equally good, or better, when compared to antibody-based detection methods. This novel detection scheme constitutes a more rapid, economical, and user-friendly alternative to many methods currently in use. Heightening both reproducibility and sensitivity, molecular imprinting in combination with surface plasmon resonance (SPR)-technology could be a versatile new tool in clinical- and research-settings alike.
journal_name
Front Cell Infect Microbioljournal_title
Frontiers in cellular and infection microbiologyauthors
Andersson T,Bläckberg A,Lood R,Ertürk Bergdahl Gdoi
10.3389/fcimb.2020.571578subject
Has Abstractpub_date
2020-11-20 00:00:00pages
571578issn
2235-2988journal_volume
10pub_type
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journal_title:Frontiers in cellular and infection microbiology
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pub_type: 杂志文章
doi:10.3389/fcimb.2017.00277
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.3389/fcimb.2017.00410
更新日期:2017-09-21 00:00:00
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2019.00021
更新日期:2019-02-15 00:00:00
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
doi:10.3389/fcimb.2019.00454
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.00044
更新日期:2020-02-28 00:00:00
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doi:10.3389/fcimb.2020.00497
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doi:10.3389/fcimb.2018.00023
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章,评审
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journal_title:Frontiers in cellular and infection microbiology
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2017.00411
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pub_type: 杂志文章,评审
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doi:10.3389/fcimb.2016.00126
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journal_title:Frontiers in cellular and infection microbiology
pub_type: 杂志文章
doi:10.3389/fcimb.2020.591974
更新日期:2020-10-29 00:00:00
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