Abstract:
OBJECTIVE:This work aimed to study the role of inflammation in medication-related osteonecrosis of the jaw (MRONJ) in rats with focus on Wnt signaling. METHODS:A total of 36 female Wistar rats (12 weeks ± 200 g) were divided into 2 groups (n = 6) in 3 experiments: saline (SAL) and zoledronic acid (ZOL). For MRONJ induction, rats received 0.1 mg/kg of ZOL (ip) 3×/week for 9 weeks. Animals from the SAL group received 0.1 mg/kg of 0.9% SAL, ip 3×/week for 9 weeks. On the 8th week, 3 left upper molars were extracted, and on the 11th week, they were euthanized. Maxillae were evaluated by macroscopic and histopathological analyses; scanning electron microscopy (SEM); immunohistochemistry for DKK-1, Wnt 10b, and caspase-3; and Raman spectrometry. Gingiva was also collected for TNF-α e IL-1β quantification. RESULTS:Bone necrosis was confirmed by healing impairment, reduced number of viable osteocytes, increased caspase-3 immunoexpression, and increased number of empty lacunae (p < 0.05). ZOL enhanced inflammation and increased gingival levels of IL-1β and TNF-α (p < 0.05). Irregular indentations were seen on bone after ZOL administration. Bone necrosis was marked by reduced amount of total and type I collagen. ZOL reduced the mineral/matrix ratio and increased carbonate/phosphate ratio. It was observed a significant reduction on Wnt10b and beta-catenin immunolabeling in the bone tissue of ZOL group. CONCLUSION:In summary, MRONJ model caused bone necrosis due to intense inflammation. Wnt signaling seems to play an important role in this process. CLINICAL RELEVANCE:New therapeutic strategies focusing on Wnt pathway can provide an interesting approach for future treatments.
journal_name
Clin Oral Investigjournal_title
Clinical oral investigationsauthors
de Sousa Ferreira VC,Lopes AP,Alves NM,Sousa FRN,Pereira KMA,Gondim DV,Girão VCC,Leitão RFC,Goes Pdoi
10.1007/s00784-020-03551-7subject
Has Abstractpub_date
2021-02-01 00:00:00pages
673-682issue
2eissn
1432-6981issn
1436-3771pii
10.1007/s00784-020-03551-7journal_volume
25pub_type
杂志文章abstract:OBJECTIVES:Resin-based composites may leach monomers such as triethylene-glycol dimethacrylate (TEGDMA), which could contribute to intrapulpal inflammation. The aim of this investigation was to examine whether various concentrations of TEGDMA are able to influence dentally relevant Matrix metalloproteinase (MMP)-2, MMP...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03545-5
更新日期:2020-08-26 00:00:00
abstract::The purpose of the present study was to decide whether composite resin or conventional glass ionomer cement should be preferred as a base material in endodontically treated premolars. Twelve extracted human maxillary premolars were mounted in a universal testing machine at a 35 degrees angle. Cuspal stiffness was dete...
journal_title:Clinical oral investigations
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s007840050049
更新日期:1998-06-01 00:00:00
abstract:OBJECTIVES:There is an increasing number of oral squamous cell carcinoma (OSCC) associated with HPV-16. However, p16 expression by immunohistochemistry as the current gold standard for a surrogate marker for virus infection reveals unsatisfying diagnostic accuracy. The aim of this study was to investigate a new rapid t...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03601-0
更新日期:2020-10-23 00:00:00
abstract:OBJECTIVES:Recent scientific evidences suggest a relevant role of the oral cavity in the transmission and pathogenicity of SARS-CoV-2. METHODS:A literature search was performed in PubMed, up to April 30, 2020, focusing on SARS-CoV-2, COVID-19, oral cavity, and antimicrobial agents. RESULTS:Oral viral load of SARS-CoV...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03413-2
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVES:Bioactive proteins are sequestered in human dentine and play a decisive role in dental pulp regeneration and repair. They can be released and exposed on the dentine surface by acids, but also chelators, such as ethylenediaminetetraacetic acid (EDTA). The objectives of this study were (i) to evaluate whether ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-1824-1
更新日期:2017-04-01 00:00:00
abstract:OBJECTIVE:This aimed to assess the potential role of chronic stress in saliva secretion, xerostomia, and oral health in a population attending a saliva clinic. MATERIALS AND METHODS:Data of 114 patients who met the inclusion criteria and completed all questionnaires were analyzed in this study. Participants completed ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2393-2
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVES:This case series compared the clinical survival of tooth-implant-supported (TI-S) and tooth-supported (T-S) three-unit fixed dental prostheses (FDPs) with zirconia frameworks and described the incidence of biological and technical complications. MATERIALS AND METHODS:Forty-four patients received 27 TI-S FDP...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1594-1
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVE:The aim of this prospective, randomized, clinical study was to assess the effect of photobiomodulation therapy (PBM) with low-level laser irradiation (LLLI) on postoperative pain after endodontic treatment. MATERIALS AND METHODS:Sixty patients, diagnosed with irreversible pulpitis in lower molar teeth, parti...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-018-2435-9
更新日期:2019-01-01 00:00:00
abstract::The present study examines the three-dimensional (3D) morphology of early approximal subsurface enamel caries lesions and subjacent dentin reactions in deciduous molars. Twenty-three extracted primary molars were embedded in Technovit 9100 and serial sections were cut using a saw microtome. Forty approximal lesions we...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840050066
更新日期:1998-12-01 00:00:00
abstract:OBJECTIVES:The aim of the present study is to provide a detailed macroscopic mapping of the palatal and tuberal blood supply applying anatomical methods and studying specific anastomoses to bridge the gap between basic structural and empirical clinical knowledge. MATERIALS AND METHODS:Ten cadavers (three dentate, seve...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2538-3
更新日期:2019-03-01 00:00:00
abstract:OBJECTIVES:The aim of the present study was to determine the prevalence of permanent first molar (P1M) extraction among Turkish adolescents and young adult subpopulation, and to investigate the effects of P1M extraction on development of the third molars (3Ms) in the same quadrant. MATERIALS AND METHODS:A retrospectiv...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1121-1
更新日期:2014-01-01 00:00:00
abstract::In the course of a prospectively designed long-term clinical trial, composite fillings and inlays were evaluated for clinical acceptability as restorative materials in one, two or more surface cavities of posterior teeth over a 1-year period. In 45 patients, 88 restorations were placed by nine student operators, under...
journal_title:Clinical oral investigations
pub_type: 临床试验,杂志文章
doi:10.1007/s007840050013
更新日期:1997-06-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the effect of fluoride dentifrices combined with sodium hexametaphosphate (HMP) on enamel demineralization in vitro. MATERIAL AND METHODS:Enamel bovine blocks were selected by initial surface hardness (SHi) and then divided into five experimental groups (n = 12): placebo...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-015-1707-x
更新日期:2016-11-01 00:00:00
abstract:INTRODUCTION:To compare, by Micro-CT and confocal laser scanning microscopy (CLSM), the ability of the Wave One Gold and Wave One systems to remove filling material from mesial canals of mandibular molars, effective time spent; quantity of extruded material, and percentage of sealer in the dentinal tubules after retrea...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2441-y
更新日期:2019-01-01 00:00:00
abstract:INTRODUCTION:Decay of teeth, erosion and disintegration of hard dental tissue are a clinically often seen side effect after radiotherapy for tumours in the head and neck region. To investigate if these side effects are due to structural changes in dental hard tissues, the current literature was searched. It can influen...
journal_title:Clinical oral investigations
pub_type: 杂志文章,评审
doi:10.1007/s00784-013-1034-z
更新日期:2014-01-01 00:00:00
abstract::The goal of this investigation was to evaluate the effect of postoperative exposure of two different bioresorbable membranes on the guided tissue regeneration (GTR) healing results compared to nonexposed sites. In each of 25 patients one pair of contralateral intrabony lesions was treated either with polylactic acid (...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s007840050021
更新日期:1997-09-01 00:00:00
abstract:OBJECTIVES:To assess the association between periodontal disease and Helicobacter pylori (H. pylori) infection in oral cavity. MATERIALS AND METHODS:We searched PubMed, Embase, Web of Science, Cochrane library, Gray literature, and clinicaltrials.gov for eligible studies up to September 25, 2019. Odds ratios (ORs) and...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析
doi:10.1007/s00784-020-03330-4
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVE:This randomized clinical trial evaluated the survival of direct restorations on first permanent molars (FPMs) with molar incisor hypomineralization (MIH) and its impact on self-reported dental pain and dental anxiety. MATERIAL AND METHOD:FPMs with MIH of 35 patients aged 7 to 16 years were included. The FPMs...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03459-2
更新日期:2020-07-21 00:00:00
abstract:OBJECTIVES:This systematic review (SR) aimed to summarise and critically appraise available evidence about the association between the synergistic consumption of alcohol and tobacco and the occurrence of oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS:Observational studies investigating the association betw...
journal_title:Clinical oral investigations
pub_type: 杂志文章,meta分析
doi:10.1007/s00784-019-02958-1
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to investigate changes in saliva concentration of the inflammatory marker MRP-8/14 and the presence of some periodontitis-associated bacteria in patients with mixed dentition treated with a rigid acrylic, bonded maxillary expander. METHODS:Fifteen patients in mixed dentition treate...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03706-6
更新日期:2020-12-03 00:00:00
abstract:OBJECTIVES:To explore the pathophysiology of proliferative verrucous leukoplakia, a rare oral disorder that exhibits high rates of recurrence and malignant transformation, through a RNAseq case-control study. MATERIAL AND METHODS:We obtained oral biopsies from 10 patients with verrucous leukoplakia lesions and from th...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03575-z
更新日期:2020-09-11 00:00:00
abstract:OBJECTIVES:It is well accepted that stage-specific embryonic antigen (SSEA)-4 is an antigen that is useful to isolate adult stem cells analogous to embryonic stem cells. Therefore, in the present study, we investigated whether SSEA-4 can also be used as a marker to identify human deciduous dental pulp (D-DP) stem cells...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1260-z
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to evaluate the effect of simulated gastric acid solution on surface topography and bending properties of esthetic coated nickel-titanium (NiTi) archwires. MATERIALS AND METHODS:Three brands of as-received white-coated superelastic NiTi upper archwires were used in this study: D...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03438-7
更新日期:2020-07-03 00:00:00
abstract:OBJECTIVES:This study aimed to investigate the effect of fracture orientation on the detection accuracy of vertical root fractures (VRFs) in non-endodontically treated teeth using four different cone beam computed tomography (CBCT) units. MATERIALS AND METHODS:Thirty eight out of 148 extracted human permanent teeth we...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-019-02905-0
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVES:The aim of this in vitro study was to evaluate the efficacy of different irrigation techniques including laser-activated irrigation using an erbium:yttrium-aluminum-garnet (Er:YAG) laser with a novel tip design (photon-induced photoacoustic streaming (PIPS)), Er:YAG laser with Preciso tip, sonic activation, ...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-016-2013-y
更新日期:2017-09-01 00:00:00
abstract:OBJECTIVES:To date, scarce evidence exists around the application of subgingival air polishing during treatment of severe periodontitis. The aim of this study was to evaluate the benefits of subgingival air polishing during non-surgical treatment of deep bleeding pockets in stages III-IV periodontitis patients MATERIAL...
journal_title:Clinical oral investigations
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00784-020-03648-z
更新日期:2021-02-01 00:00:00
abstract:OBJECTIVES:This study aimed to examine the electromyographic activity of superficial masseter and anterior temporal muscles during chewing gum and gummy jelly mastication in healthy subjects to reveal the difference of neuromuscular control of jaw-closing muscles, according to the food texture. MATERIALS AND METHODS:E...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-018-2754-x
更新日期:2019-09-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to establish the prevalence of celiac disease (CD) in patients with erosive and reticular form of oral lichen planus (OLP) compared to healthy controls. MATERIALS AND METHODS:Tissue transglutaminase antibodies (anti-tTG) and antibodies against gliadin (AGA) were measured in the ser...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-014-1288-0
更新日期:2015-04-01 00:00:00
abstract:OBJECTIVES:To assess the internal and external morphologies of the mesiobuccal (MB) root of maxillary molars presenting a third root canal (MB3), using micro-computed tomography (micro-CT). MATERIAL AND METHODS:Two-hundred and sixty-five extracted maxillary first and second molars with different root configurations we...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-020-03284-7
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVES:The main goal of the present study was to compare the biomechanical stability of locking plates and conventional miniplate combinations in human mandibles reconstructed with fibular grafts. MATERIALS AND METHODS:A specially developed and well-proven testing device reproduced the in vivo loading conditions o...
journal_title:Clinical oral investigations
pub_type: 杂志文章
doi:10.1007/s00784-013-1105-1
更新日期:2014-05-01 00:00:00