Abstract:
Background:Type 2 diabetes mellitus (T2DM) had been reported to be associated with tendinopathy. However, the underlying mechanisms of diabetic tendinopathy still remain largely to be discovered. The purpose of this study was to develop insulin resistance (IR) model on primary human tenocytes (hTeno) culture with tumour necrosis factor-alpha (TNF-α) treatment to study tenocytes homeostasis as an implication for diabetic tendinopathy. Methods:hTenowere isolated from human hamstring tendon. Presence of insulin receptor beta (INSR-β) on normal tendon tissues and the hTeno monolayer culture were analyzed by immunofluorescence staining. The presence of Glucose Transporter Type 1 (GLUT1) and Glucose Transporter Type 4 (GLUT4) on the hTeno monolayer culture were also analyzed by immunofluorescence staining. Primary hTeno were treated with 0.008, 0.08, 0.8 and 8.0 µM of TNF-α, with and without insulin supplement. Outcome measures include 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-d-glucose (2-NBDG) assay to determine the glucose uptake activity; colourimetric total collagen assay to quantify the total collagen expression levels; COL-I ELISA assay to measure the COL-I expression levels and real-time qPCR to analyze the mRNA gene expressions levels of Scleraxis (SCX), Mohawk (MKX), type I collagen (COL1A1), type III collagen (COL3A1), matrix metalloproteinases (MMP)-9 and MMP-13 in hTeno when treated with TNF-α. Apoptosis assay for hTeno induced with TNF-α was conducted using Annexin-V FITC flow cytometry analysis. Results:Immunofluorescence imaging showed the presence of INSR-β on the hTeno in the human Achilles tendon tissues and in the hTeno in monolayer culture. GLUT1 and GLUT4 were both positively expressed in the hTeno. TNF-α significantly reduced the insulin-mediated 2-NBDG uptake in all the tested concentrations, especially at 0.008 µM. Total collagen expression levels and COL-I expression levels in hTeno were also significantly reduced in hTeno treated with 0.008 µM of TNF-α. The SCX, MKX and COL1A1 mRNA expression levels were significantly downregulated in all TNF-α treated hTeno, whereas the COL3A1, MMP-9 and MMP-13 were significantly upregulated in the TNF-α treated cells. TNF-α progressively increased the apoptotic cells at 48 and 72 h. Conclusion:At 0.008 µM of TNF-α, an IR condition was induced in hTeno, supported with the significant reduction in glucose uptake, as well as significantly reduced total collagen, specifically COL-I expression levels, downregulation of candidate tenogenic markers genes (SCX and MKX), and upregulation of ECM catabolic genes (MMP-9 and MMP-13). Development of novel IR model in hTeno provides an insight on how tendon homeostasis could be affected and can be used as a tool for further discovering the effects on downstream molecular pathways, as the implication for diabetic tendinopathy.
journal_name
PeerJjournal_title
PeerJauthors
Tan HY,Tan SL,Teo SH,Roebuck MM,Frostick SP,Kamarul Tdoi
10.7717/peerj.8740subject
Has Abstractpub_date
2020-06-08 00:00:00pages
e8740issn
2167-8359pii
8740journal_volume
8pub_type
杂志文章相关文献
PeerJ文献大全abstract::Fear is a response to a known threat, anxiety is a response to a perceived threat. Both of these affective states can be detrimental to animal welfare in modern housing environments. In comparison to the well-validated tests for assessing fear in laying hens, tests for measuring anxiety are less developed. Perception ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7303
更新日期:2019-07-10 00:00:00
abstract::Relative gene expression data obtained from quantitative RT-PCR (RT-qPCR) experiments are dependent on appropriate normalization to represent true values. It is common to use constitutively expressed endogenous reference genes (RGs) for normalization, but for this strategy to be valid the RGs must be stably expressed ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7181
更新日期:2019-06-26 00:00:00
abstract::Miniature inverted-repeat transposable elements (MITEs) have been associated with genic regions in plant genomes and may play important roles in the regulation of nearby genes via recruitment of small RNAs (sRNA) to the MITEs loci. We identified eight families of MITEs in the sugarcane genome assembly with MITE-Hunter...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6080
更新日期:2019-01-11 00:00:00
abstract:BACKGROUND:Algae encompass a wide array of photosynthetic organisms that are ubiquitously distributed in aquatic and terrestrial habitats. Algal species often bloom in aquatic ecosystems, providing a significant autochthonous carbon input to the deeper anoxic layers in stratified water bodies. In addition, various alga...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2803
更新日期:2017-01-10 00:00:00
abstract:Background:This study sought to better understand the psychometric properties of the SARC-F, by examining the baseline and training-related relationships between the five SARC-F items and objective measures of muscle function. Each of the five items of the SARC-F are scored from 0 to 2, with total score of four or more...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.8140
更新日期:2019-11-27 00:00:00
abstract::The objective of this study was to quantify consumption of clothianidin-treated corn seed by birds following standard planting practices. Based on post-planting seed counts on 21 fields in southwestern Ontario, Canada, between 29 and 813 seeds/ha (mean of 224 ± 167 (SD)) were estimated to remain on the soil surface im...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.5880
更新日期:2018-11-07 00:00:00
abstract::Aerosol optical depth (AOD) is a key parameter that reflects aerosol characteristics. However, research on the AOD of dust aerosols and various environmental variables is scarce. Therefore, we conducted in-depth studies on the distributions and variations of AOD in the Taklimakan Desert and its margins, China. We exam...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.10542
更新日期:2021-01-05 00:00:00
abstract::Background. In recent years, there has been an explosion in the number of technical and medical diagnostic platforms being developed. This has greatly improved our ability to more accurately, and more comprehensively, explore and characterize human biological systems on the individual level. Large quantities of biomed...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.177
更新日期:2013-10-03 00:00:00
abstract::The purpose of the study was to investigate the relationship between different exercise modes and visuospatial working memory in healthy older adults. A cross-sectional design was adopted. A total of 111 healthy older adults were enrolled in the study. They were classified by the exercise-related questionnaire to be i...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2254
更新日期:2016-07-20 00:00:00
abstract:Background:Staphylococcus aureus is a human colonizer with high potential for virulence, and the spread of the virulent strains from the colonized hosts to non-carriers in the community is on the increase. However, there are few reports on comprehensive analysis of staphylococcal enterotoxin (SE) genes with clonal line...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.5204
更新日期:2018-07-09 00:00:00
abstract::The goal of this study was to assess the diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis. Blood samples were collected from 56 sheep (36 naturally infected with Pasteurella multocida and 20 healthy controls) belonging to one farm in Eastern region, Saudi...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.2161
更新日期:2016-07-19 00:00:00
abstract::Limestone karsts of Southeast Asia can harbor high levels of endemism, but are highly fragmented, increasingly threatened, and their biodiversity is often poorly studied. This is true of the Padawan Limestone Area of Sarawak, Malaysia, home to the endemic Artocarpus annulatus, the closest known wild relative of two im...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.9897
更新日期:2020-09-21 00:00:00
abstract::Artifacts removal and rhythms extraction from electroencephalography (EEG) signals are important for portable and wearable EEG recording devices. Incorporating a novel grouping rule, we proposed an adaptive singular spectrum analysis (SSA) method for artifacts removal and rhythms extraction. Based on the EEG signal am...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3474
更新日期:2017-06-28 00:00:00
abstract:Background:Hair follicles in cashmere goats are divided into primary and secondary hair follicles (HFs). HF development, which determines the morphological structure, is regulated by a large number of vital genes; however, the key functional genes and their interaction networks are still unclear. Although the vitamin D...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7230
更新日期:2019-07-03 00:00:00
abstract:Background:B-mode ultrasound is one of the most commonly used imaging techniques for evaluating thyroid nodules due to its noninvasive property and excellent performance in terms of discriminating between benign and malignant nodules. However, the accuracy of differential diagnosis strongly depends on the experience of...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.9112
更新日期:2020-05-04 00:00:00
abstract::Bacterial vaginosis (BV) is one of most common vaginal infections. However, its diagnosis by classical methods reveals low specificity. Our goal was to evaluate the accuracy diagnosis of 150 vaginal samples with research gold standard methods and our Peptide Nucleic Acid (PNA) probes by Fluorescence in situ Hybridizat...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.780
更新日期:2015-02-17 00:00:00
abstract:Background:Sex ratio distorting agents (maternally inherited symbionts and meiotically-driving sex chromosomes) are common in insects. When these agents rise to high frequencies they create strong population sex ratio bias and selection then favours mutations that act to restore the rare sex. Despite this strong select...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7677
更新日期:2019-10-01 00:00:00
abstract:Background:Vegetation in the Mongolian Plateau is very sensitive to climate change, which has a significant impact on the regulation of terrestrial carbon cycle. Methods:We analyzed spatio-temporal changes of both growing season and the seasonal Normalized Difference Vegetation Index (NDVI) using simple linear trend a...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7735
更新日期:2019-09-30 00:00:00
abstract::The role of glial cells in pain modulation has recently gathered attention. The objective of this study was to determine healthy spinal microglia and astrocyte morphology and disposition in equine spinal cord dorsal horns using Iba-1 and GFAP/Cx-43 immunofluorescence labeling, respectively. Five adult horses without v...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.3965
更新日期:2017-10-27 00:00:00
abstract:Background:Cities face rapid changes leading to increasing inequalities and emerging public health issues that require cost-effective interventions. The urban exposome concept refers to the continuous monitoring of urban environmental and health indicators using the city and smaller intra-city areas as measurement unit...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6851
更新日期:2019-05-24 00:00:00
abstract::Researchers often need to consider the practical significance of a relationship. For example, interpreting the magnitude of an effect size or establishing bounds in equivalence testing requires knowledge of the meaningfulness of a relationship. However, there has been little research exploring the degree of relationsh...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6853
更新日期:2019-05-14 00:00:00
abstract::Seymouria is the best known of the seymouriamorphs, a group of Permo-Carboniferous reptiliomorphs with both terrestrial and aquatic taxa. The majority of research on Seymouria has focused on cranial anatomy, with few detailed descriptions or illustrations of the postcrania. We utilized neutron computed tomography (nCT...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.8698
更新日期:2020-03-10 00:00:00
abstract::Soil microbial communities are continuously exposed to H2 diffusing into the soil from the atmosphere. N2-fixing nodules represent a peculiar microniche in soil where H2 can reach concentrations up to 20,000 fold higher than in the global atmosphere (0.530 ppmv). In this study, we investigated the impact of H2 exposur...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1782
更新日期:2016-03-10 00:00:00
abstract:Background:Despite clinical trials with antioxidant supplementation, few studies have been conducted to evaluate the nutritional status of antioxidant vitamins and minerals, and none have reported on the status of these serum antioxidants associated with the dietary intake of antioxidants by non-alcoholic fatty liver d...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.9838
更新日期:2020-09-17 00:00:00
abstract::Meniscal tears are a common cause of stifle lameness in dogs. Use of autologous synoviocytes from the affected stifle is an attractive cell source for tissue engineering replacement fibrocartilage. However, the diseased state of these cells may impede in vitro fibrocartilage formation. Synoviocytes from 12 osteoarthri...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.581
更新日期:2014-09-30 00:00:00
abstract:BACKGROUND:The purpose of this study is to provide a report on scientific production during the period 2010-2014 in order to identify the major topics as well as the predominant actors (journals, countries, continents) involved in the field of eye disease. METHODS:A PubMed search was carried out to extract articles re...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.1557
更新日期:2016-01-07 00:00:00
abstract:Background:Conserving both biodiversity and ecosystem services is a major goal of the Convention on Biological Diversity. Hotspots for biodiversity in the Andes significantly overlap with areas with dense human populations that sustain their economy through agricultural production. Therefore, developing management form...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6207
更新日期:2019-02-13 00:00:00
abstract::A greenhouse experiment was carried out to investigate the effects of arbuscular mycorrhizal fungi (AMF) on the growth, P and Cd concentrations and bioenergy quality-related factors of five cultivars of switchgrass, including three lowland cultivars (Alamo (Ala), Kanlow (Kan), Performer (Per)) and two highland cultiva...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.4425
更新日期:2018-03-06 00:00:00
abstract::A new genus with a new species of the tribe Hoplocampini of Hoplocampinae was described from China: Analcellicampa xanthosoma Wei & Niu, gen. et sp. nov.Hoplocampa danfengensis G. Xiao 1994 was designated as the type species of the new genus. The characters of Analcellicampa danfengensis (G. Xiao) comb. nov. were brie...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.6866
更新日期:2019-05-02 00:00:00
abstract::Transgenerational effects are important for phenotypic plasticity and adaptation of marine invertebrates in the changing ocean. Ultraviolet-B (UV-B) radiation is an increasing threat to marine invertebrates. For the first time, we reported positive and negative transgenerational effects of UV-B radiation on egg size, ...
journal_title:PeerJ
pub_type: 杂志文章
doi:10.7717/peerj.7598
更新日期:2019-08-26 00:00:00