Abstract:
BACKGROUND:Pumilio RNA-binding proteins are evolutionarily conserved throughout eukaryotes and are involved in RNA decay, transport, and translation repression in the cytoplasm. Although a majority of Pumilio proteins function in the cytoplasm, two nucleolar forms have been reported to have a function in rRNA processing in Arabidopsis. The species of the genus Chara have been known to be most closely related to land plants, as they share several characteristics with modern Embryophyta. RESULTS:In this study, we identified two putative nucleolar Pumilio protein genes, namely, ChPUM2 and ChPUM3, from the transcriptome of Chara corallina. Of the two ChPUM proteins, ChPUM2 was most similar in amino acid sequence (27% identity and 45% homology) and predicted protein structure to Arabidopsis APUM23, while ChPUM3 was similar to APUM24 (35% identity and 54% homology). The transient expression of 35S:ChPUM2-RFP and 35S:ChPUM3-RFP showed nucleolar localization of fusion proteins in tobacco leaf cells, similar to the expression of 35S:APUM23-GFP and 35S:APUM24-GFP. Moreover, 35S:ChPUM2 complemented the morphological defects of the apum23 phenotypes but not those of apum24, while 35S:ChPUM3 could not complement the apum23 and apum24 mutants. Similarly, the 35S:ChPUM2/apum23 plants rescued the pre-rRNA processing defect of apum23, but 35S:ChPUM3/apum24+/- plants did not rescue that of apum24. Consistent with these complementation results, a known target RNA-binding sequence at the end of the 18S rRNA (5'-GGAAUUGACGG) for APUM23 was conserved in Arabidopsis and C. corallina, whereas a target region of ITS2 pre-rRNA for APUM24 was 156 nt longer in C. corallina than in A. thaliana. Moreover, ChPUM2 and APUM23 were predicted to have nearly identical structures, but ChPUM3 and APUM24 have different structures in the 5th C-terminal Puf RNA-binding domain, which had a longer random coil in ChPUM3 than in APUM24. CONCLUSIONS:ChPUM2 of C. corallina was functional in Arabidopsis, similar to APUM23, but ChPUM3 did not substitute for APUM24 in Arabidopsis. Protein homology modeling showed high coverage between APUM23 and ChPUM2, but displayed structural differences between APUM24 and ChPUM3. Together with the protein structure of ChPUM3 itself, a short ITS2 of Arabidopsis pre-rRNA may interrupt the binding of ChPUM3 to 3'-extended 5.8S pre-rRNA.
journal_name
BMC Plant Bioljournal_title
BMC plant biologyauthors
Park SH,Kim HS,Kalita PJ,Choi SBdoi
10.1186/s12870-020-02444-xsubject
Has Abstractpub_date
2020-05-24 00:00:00pages
230issue
1issn
1471-2229pii
10.1186/s12870-020-02444-xjournal_volume
20pub_type
杂志文章abstract:BACKGROUND:Identification of loci for grain yield (GY) and related traits, and dissection of the genetic architecture are important for yield improvement through marker-assisted selection (MAS). Two genome-wide association study (GWAS) methods were used on a diverse panel of 166 elite wheat varieties from the Yellow an...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1781-3
更新日期:2019-04-29 00:00:00
abstract:BACKGROUND:For most cruciferous plants, which are known as important crops and a number of weeds, hybrid breeding is hampered by the unavailability of a pollination control system. Male sterility induced by a gametocide can be useful for the utilization of plant heterosis. RESULTS:The gametocidal effect of sulfonylure...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-017-1019-1
更新日期:2017-06-01 00:00:00
abstract:BACKGROUND:Chromatin dynamics and stability are both required to control normal development of multicellular organisms. Chromatin assembly factor CAF-1 is a histone chaperone that facilitates chromatin formation and the maintenance of specific chromatin states. In plants and animals CAF-1 is essential for normal develo...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-8-54
更新日期:2008-05-13 00:00:00
abstract:BACKGROUND:Transcription factors play the crucial rule of regulating gene expression and influence almost all biological processes. Systematically identifying and annotating transcription factors can greatly aid further understanding their functions and mechanisms. In this article, we present SoyDB, a user friendly dat...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-14
更新日期:2010-01-18 00:00:00
abstract:BACKGROUND:Soil salinity is an important factor affecting growth, development, and productivity of almost all land plants, including the forage crop alfalfa (Medicago sativa). However, little is known about how alfalfa responds and adapts to salt stress, particularly among different salt-tolerant cultivars. RESULTS:Am...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1250-4
更新日期:2018-02-15 00:00:00
abstract:BACKGROUND:Pinus massoniana Lamb. is an important afforestation tree species with high economic, ecological and medicinal values. Aluminum (Al) toxicity driven by soil acidification causes dieback of P. massoniana plantations. Previous studies showed that ectomycorrhizal fungi alleviate Al stress damages in Pinus, but ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-02719-3
更新日期:2020-11-16 00:00:00
abstract:BACKGROUND:Aromatic rice is popular worldwide because of its characteristic fragrance. Genetic studies and physical fine mapping reveal that a candidate gene (fgr/OsBADH2) homologous to betaine aldehyde dehydrogenase is responsible for aroma metabolism in fragrant rice varieties, but the direct evidence demonstrating t...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-8-100
更新日期:2008-10-08 00:00:00
abstract:BACKGROUND:The acid component of grape berries, originating in the metabolism of malate and tartrate, the latter being less well-known than the former, is a key factor at play in the microbiological stability of wines destined for distillation. Grape acidity is increasingly affected by climate changes. The ability to c...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-016-0833-1
更新日期:2016-06-28 00:00:00
abstract:BACKGROUND:Pear fruit exhibit a single sigmoid pattern during development, while peach and strawberry fruits exhibit a double sigmoid pattern. However, little is known about the differences between these two patterns. RESULTS:In this study, fruit weights were measured and paraffin sections were made from fruitlet to m...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-2317-6
更新日期:2020-03-06 00:00:00
abstract:BACKGROUND:Good establishment is important for rapid leaf area development in wheat crops. Poor establishment results in fewer, later-emerging plants, reduced leaf area and tiller number. In addition, poorly established crops suffer from increased soil moisture loss through evaporation and greater competition from weed...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1919-3
更新日期:2019-07-29 00:00:00
abstract:BACKGROUND:Parthenium argentatum (guayule) is an industrial crop that produces latex, which was recently commercialized as a source of latex rubber safe for people with Type I latex allergy. The complete plastid genome of P. argentatum was sequenced. The sequence provides important information useful for genetic engine...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-9-131
更新日期:2009-11-17 00:00:00
abstract:BACKGROUND:Nitrogen is one basic element of amino acids and grain protein in wheat. In field experiments, wheat plants were subjected to different timing of nitrogen topdressing treatments: at the stages of emergence of the top fifth leaf (TL5), top third leaf (TL3) and top first leaf (TL1) to test the regulatory effec...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1563-3
更新日期:2018-12-13 00:00:00
abstract:BACKGROUND:Cotton fibre quality traits such as fibre length, strength, and degree of maturation are determined by genotype and environment during the sequential phases of cotton fibre development (cell elongation, transition to secondary cell wall construction and cellulose deposition). The cotton fibre middle lamella ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-017-1017-3
更新日期:2017-03-31 00:00:00
abstract:BACKGROUND:Soybean (Glycine max (L.)) is one the most important oil-yielding cash crops. However, the soybean production has been seriously restricted by salinization. It is therefore crucial to identify salt tolerance-related genes and reveal molecular mechanisms underlying salt tolerance in soybean crops. A better un...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-2084-4
更新日期:2019-11-05 00:00:00
abstract:BACKGROUND:Wheat grain avenin-like proteins (ALPs) belong to a recently discovered class of wheat grain storage protein. ALPs in wheat grains not only have beneficial effects on dough quality but also display antifungal activities, which is a novel observation for wheat storage proteins. Previous studies have shown tha...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-2259-z
更新日期:2020-01-29 00:00:00
abstract:BACKGROUND:Unilateral incompatibility (UI) is an asymmetric reproductive barrier that unidirectionally prevents gene flow between species and/or populations. UI is characterized by a compatible interaction between partners in one direction, but in the reciprocal cross fertilization fails, generally due to pollen tube r...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-017-1032-4
更新日期:2017-04-24 00:00:00
abstract:BACKGROUND:In mammals, nucleostemin (NS), a nucleolar GTPase, is involved in stem cell proliferation, embryogenesis and ribosome biogenesis. Arabidopsis NUCLEOSTEMIN-LIKE 1 (NSN1) has previously been shown to be essential for plant growth and development. However, the role of NSN1 in cell proliferation is largely unkno...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1289-2
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:When photosynthetic organisms are exposed to harsh environmental conditions such as high light intensities or cold stress, the production of reactive oxygen species like singlet oxygen is stimulated in the chloroplast. In Chlamydomonas reinhardtii singlet oxygen was shown to act as a specific signal inducing...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-279
更新日期:2010-12-17 00:00:00
abstract:BACKGROUND:The sucrosylgalactoside oligosaccharide raffinose (Raf, Suc-Gal1) accumulates in Arabidopsis leaves in response to a myriad of abiotic stresses. Whilst galactinol synthases (GolS), the first committed enzyme in Raf biosynthesis are well characterised in Arabidopsis, little is known of the second biosynthetic...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-218
更新日期:2013-12-20 00:00:00
abstract:BACKGROUND:The development of chilling and freezing injury symptoms in plants is known to frequently coincide with peroxidation of free fatty acids. Mitochondria are one of the major sources of reactive oxygen species during cold stress. Recently it has been suggested that uncoupling of oxidation and phosphorylation in...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-1-1
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:The thylakoid system in plant chloroplasts is organized into two distinct domains: grana arranged in stacks of appressed membranes and non-appressed membranes consisting of stroma thylakoids and margins of granal stacks. It is argued that the reason for the development of appressed membranes in plants is tha...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-12-72
更新日期:2012-05-25 00:00:00
abstract:BACKGROUND:MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level in eukaryotes. In rice, MIR7695 expression is regulated by infection with the rice blast fungus Magnaporthe oryzae with subsequent down-regulation of an alternatively spliced transcript of natural res...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-2156-5
更新日期:2019-12-18 00:00:00
abstract:BACKGROUND:Tocopherols are natural antioxidants with both in vivo (vitamin E) and in vitro activity. Sunflower seeds contain predominantly alpha-tocopherol (>90% of total tocopherols), with maximum vitamin E effect but lower in vitro antioxidant action than other tocopherol forms such as gamma-tocopherol. Sunflower ger...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-12-71
更新日期:2012-05-18 00:00:00
abstract:BACKGROUND:Auxin-induced genes regulate many aspects of plant growth and development. The Gretchen Hagen 3 (GH3) gene family, one of three major early auxin-responsive families, is ubiquitous in the plant kingdom and its members function as regulators in modulating hormonal homeostasis, and stress adaptations. Specific...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1545-5
更新日期:2018-12-12 00:00:00
abstract:BACKGROUND:Arabidopsis ovules comprise four morphologically distinct parts: the nucellus, which contains the embryo sac, two integuments that become the seed coat, and the funiculus that anchors the ovule within the carpel. Analysis of developmental mutants has shown that ovule morphogenesis relies on tightly regulated...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-9-29
更新日期:2009-03-16 00:00:00
abstract:BACKGROUND:Protoplast technologies offer unique opportunities for fundamental research and to develop novel germplasm through somatic hybridization, organelle transfer, protoclonal variation, and direct insertion of DNA. Applying protoplast technologies to develop Dutch elm disease resistant American elms (Ulmus americ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-12-75
更新日期:2012-05-30 00:00:00
abstract:BACKGROUND:Characterization of the expression and function of genes regulating embryo development in conifers is interesting from an evolutionary point of view. However, our knowledge about the regulation of embryo development in conifers is limited. During early embryo development in Pinus species the proembyo goes th...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-016-0939-5
更新日期:2016-11-18 00:00:00
abstract:BACKGROUND:During asexual reproduction the embryogenic callus can differentiate into a new plantlet, offering great potential for fostering in vitro culture efficiency in plants. The immature embryos (IMEs) of wheat (Triticum aestivum L.) are more easily able to generate embryogenic callus than mature embryos (MEs). To...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-017-1204-2
更新日期:2017-12-19 00:00:00
abstract:BACKGROUND:Flowering at an appropriate time is crucial for seed maturity and reproductive success in all flowering plants. Soybean (Glycine max) is a typical short day plant, and both photoperiod and autonomous pathway genes exist in soybean genome. However, little is known about the functions of soybean autonomous pat...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-014-0263-x
更新日期:2014-10-07 00:00:00
abstract:BACKGROUND:The medicinal plant Artemisia annua is covered with filamentous trichomes and glandular, artemisinin producing trichomes. A high artemisinin supply is needed at a reduced cost for treating malaria. Artemisinin production in bioreactors can be facilitated if a better insight is obtained in the biosynthesis of...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-220
更新日期:2013-12-20 00:00:00