Differential transcriptome analysis of glandular and filamentous trichomes in Artemisia annua.

Abstract:

BACKGROUND:The medicinal plant Artemisia annua is covered with filamentous trichomes and glandular, artemisinin producing trichomes. A high artemisinin supply is needed at a reduced cost for treating malaria. Artemisinin production in bioreactors can be facilitated if a better insight is obtained in the biosynthesis of artemisinin and other metabolites. Therefore, metabolic activities of glandular and filamentous trichomes were investigated at the transcriptome level. RESULTS:By laser pressure catapulting, glandular and filamentous trichomes as well as apical and sub-apical cells from glandular trichomes were collected and their transcriptome was sequenced using Illumina RNA-Seq. A de novo transcriptome was assembled (Trinity) and studied with a differential expression analysis (edgeR).A comparison of the transcriptome from glandular and filamentous trichomes shows that MEP, MVA, most terpene and lipid biosynthesis pathways are significantly upregulated in glandular trichomes. Conversely, some transcripts coding for specific sesquiterpenoid and triterpenoid enzymes such as 8-epi-cedrol synthase and an uncharacterized oxidosqualene cyclase were significantly upregulated in filamentous trichomes. All known artemisinin biosynthesis genes are upregulated in glandular trichomes and were detected in both the apical and sub-apical cells of the glandular trichomes. No significant differential expression could be observed between the apical and sub-apical cells. CONCLUSIONS:Our results underscore the vast metabolic capacities of A. annua glandular trichomes but nonetheless point to the existence of specific terpene metabolic pathways in the filamentous trichomes. Candidate genes that might be involved in artemisinin biosynthesis are proposed based on their putative function and their differential expression level.

journal_name

BMC Plant Biol

journal_title

BMC plant biology

authors

Soetaert SS,Van Neste CM,Vandewoestyne ML,Head SR,Goossens A,Van Nieuwerburgh FC,Deforce DL

doi

10.1186/1471-2229-13-220

subject

Has Abstract

pub_date

2013-12-20 00:00:00

pages

220

issn

1471-2229

pii

1471-2229-13-220

journal_volume

13

pub_type

杂志文章
  • Ionomic and metabolic responses to neutral salt or alkaline salt stresses in maize (Zea mays L.) seedlings.

    abstract:BACKGROUND:Soil salinity and alkalinity present a serious threat to global agriculture. However, most of the studies have focused on neutral salt stress, and the information on the metabolic responses of plants to alkaline salt stress is limited. This investigation aimed at determining the influence of neutral salt and...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-017-0994-6

    authors: Guo R,Shi L,Yan C,Zhong X,Gu F,Liu Q,Xia X,Li H

    更新日期:2017-02-10 00:00:00

  • Genome-wide association study of resistance to stripe rust (Puccinia striiformis f. sp. tritici) in Sichuan wheat.

    abstract:BACKGROUND:Stripe rust (also called yellow rust) is a common and serious fungal disease of wheat (Triticum aestivum L.) caused by Puccinia striiformis f. sp. tritici. The narrow genetic basis of modern wheat cultivars and rapid evolution of the rust pathogen have been responsible for periodic and devastating epidemics ...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-019-1764-4

    authors: Ye X,Li J,Cheng Y,Yao F,Long L,Yu C,Wang Y,Wu Y,Li J,Wang J,Jiang Q,Li W,Ma J,Wei Y,Zheng Y,Chen G

    更新日期:2019-04-16 00:00:00

  • Expression and testing in plants of ArcLight, a genetically-encoded voltage indicator used in neuroscience research.

    abstract:BACKGROUND:It is increasingly appreciated that electrical controls acting at the cellular and supra-cellular levels influence development and initiate rapid responses to environmental cues. An emerging method for non-invasive optical imaging of electrical activity at cell membranes uses genetically-encoded voltage indi...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-015-0633-z

    authors: Matzke AJ,Matzke M

    更新日期:2015-10-12 00:00:00

  • Exposure to trace amounts of sulfonylurea herbicide tribenuron-methyl causes male sterility in 17 species or subspecies of cruciferous plants.

    abstract:BACKGROUND:For most cruciferous plants, which are known as important crops and a number of weeds, hybrid breeding is hampered by the unavailability of a pollination control system. Male sterility induced by a gametocide can be useful for the utilization of plant heterosis. RESULTS:The gametocidal effect of sulfonylure...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-017-1019-1

    authors: Yu CY,Dong JG,Hu SW,Xu AX

    更新日期:2017-06-01 00:00:00

  • Abscisic acid synergizes with sucrose to enhance grain yield and quality of rice by improving the source-sink relationship.

    abstract:BACKGROUND:Abscisic acid (ABA) and sucrose act as molecular signals in response to abiotic stress. However, how their synergy regulates the source-sink relationship has rarely been studied. This study aimed to reveal the mechanism underlying the synergy between ABA and sucrose on assimilates allocation to improve grain...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-019-2126-y

    authors: Chen T,Li G,Islam MR,Fu W,Feng B,Tao L,Fu G

    更新日期:2019-11-27 00:00:00

  • An investigation on possible effect of leaching fractions physiological responses of hot pepper plants to irrigation water salinity.

    abstract:BACKGROUND:The modification effect of leaching fraction (LF) on the physiological responses of plants to irrigation water salinity (ECiw) remains unknown. Here, leaf gas exchange, photosynthetic light-response and CO2-response curves, and total carbon (C) and nitrogen (N) accumulation in hot pepper leaves were investig...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-019-1910-z

    authors: Qiu R,Liu C,Li F,Wang Z,Yang Z,Cui N

    更新日期:2019-07-08 00:00:00

  • Down-regulation of POLYGALACTURONASE1 alters firmness, tensile strength and water loss in apple (Malus x domestica) fruit.

    abstract:BACKGROUND:While there is now a significant body of research correlating apple (Malus x domestica) fruit softening with the cell wall hydrolase ENDO-POLYGALACTURONASE1 (PG1), there is currently little knowledge of its physiological effects in planta. This study examined the effect of down regulation of PG1 expression i...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-12-129

    authors: Atkinson RG,Sutherland PW,Johnston SL,Gunaseelan K,Hallett IC,Mitra D,Brummell DA,Schröder R,Johnston JW,Schaffer RJ

    更新日期:2012-08-02 00:00:00

  • Long-distance transport of Gibberellic Acid Insensitive mRNA in Nicotiana benthamiana.

    abstract:BACKGROUND:The Gibberellic Acid (GA) signal is governed by the GAI (Gibberellic Acid Insensitive) repressor, which is characterized by a highly conserved N-terminal DELLA domain. Deletion of the DELLA domain results in constitutive suppression of GA signaling. As the GAI transcript is transportable in phloem elements, ...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-13-165

    authors: Xu H,Iwashiro R,Li T,Harada T

    更新日期:2013-10-21 00:00:00

  • The HaDREB2 transcription factor enhances basal thermotolerance and longevity of seeds through functional interaction with HaHSFA9.

    abstract:BACKGROUND:Transcription factor HaDREB2 was identified in sunflower (Helianthus annuus L.) as a drought-responsive element-binding factor 2 (DREB2) with unique properties. HaDREB2 and the sunflower Heat Shock Factor A9 (HaHSFA9) co-activated the Hahsp17.6G1 promoter in sunflower embryos. Both factors could be involved ...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-9-75

    authors: Almoguera C,Prieto-Dapena P,Díaz-Martín J,Espinosa JM,Carranco R,Jordano J

    更新日期:2009-06-19 00:00:00

  • Comparative analysis of maca (Lepidium meyenii) proteome profiles reveals insights into response mechanisms of herbal plants to high-temperature stress.

    abstract:BACKGROUND:High-temperature stress (HTS) is one of the main environmental stresses that limit plant growth and crop production in agricultural systems. Maca (Lepidium meyenii) is an important high-altitude herbaceous plant adapted to a wide range of environmental stimuli such as cold, strong wind and UV-B exposure. How...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-02645-4

    authors: Wang ZQ,Zhao QM,Zhong X,Xiao L,Ma LX,Wu CF,Zhang Z,Zhang LQ,Tian Y,Fan W

    更新日期:2020-09-16 00:00:00

  • ABC1K10a, an atypical kinase, functions in plant salt stress tolerance.

    abstract:BACKGROUND:ABC1K (Activity of BC1 complex Kinase) is an evolutionarily primitive atypical kinase family widely distributed among prokaryotes and eukaryotes. The ABC1K protein kinases in Arabidopsis are predicted to localize either to the mitochondria or chloroplasts, in which plastid-located ABC1K proteins are involved...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-02467-4

    authors: Qin X,Duan Z,Zheng Y,Liu WC,Guo S,Botella JR,Song CP

    更新日期:2020-06-10 00:00:00

  • Structural and functional similarities and differences in nucleolar Pumilio RNA-binding proteins between Arabidopsis and the charophyte Chara corallina.

    abstract:BACKGROUND:Pumilio RNA-binding proteins are evolutionarily conserved throughout eukaryotes and are involved in RNA decay, transport, and translation repression in the cytoplasm. Although a majority of Pumilio proteins function in the cytoplasm, two nucleolar forms have been reported to have a function in rRNA processin...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-02444-x

    authors: Park SH,Kim HS,Kalita PJ,Choi SB

    更新日期:2020-05-24 00:00:00

  • Ectopic expression of the apple nucleus-encoded thylakoid protein MdY3IP1 triggers early-flowering and enhanced salt-tolerance in Arabidopsis thaliana.

    abstract:BACKGROUND:The roles in photosystem I (PSI) assembly of the nucleus-encoded thylakoid protein Y3IP1 who interacts with the plastid-encoded Ycf3 protein that has been well-characterized in plants. However, its function and potential mechanisms in other aspects remain poorly understood. RESULTS:We identified the apple M...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-018-1232-6

    authors: Yu JQ,Wang JH,Sun CH,Zhang QY,Hu DG,Hao YJ

    更新日期:2018-01-20 00:00:00

  • Genome-wide meta-analysis of maize heterosis reveals the potential role of additive gene expression at pericentromeric loci.

    abstract:BACKGROUND:The identification of QTL involved in heterosis formation is one approach to unravel the not yet fully understood genetic basis of heterosis - the improved agronomic performance of hybrid F1 plants compared to their inbred parents. The identification of candidate genes underlying a QTL is important both for ...

    journal_title:BMC plant biology

    pub_type: 杂志文章,meta分析

    doi:10.1186/1471-2229-14-88

    authors: Thiemann A,Fu J,Seifert F,Grant-Downton RT,Schrag TA,Pospisil H,Frisch M,Melchinger AE,Scholten S

    更新日期:2014-04-02 00:00:00

  • Comparative phosphoproteomic analysis of blast resistant and susceptible rice cultivars in response to salicylic acid.

    abstract:BACKGROUND:Salicylic acid (SA) is a significant signaling molecule that induces rice resistance against pathogen invasion. Protein phosphorylation carries out an important regulatory function in plant defense responses, while the global phosphoproteome changes in rice response to SA-mediated defense response has not be...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-019-2075-5

    authors: Sun R,Qin S,Zhang T,Wang Z,Li H,Li Y,Nie Y

    更新日期:2019-10-28 00:00:00

  • A blackberry (Rubus L.) expressed sequence tag library for the development of simple sequence repeat markers.

    abstract:BACKGROUND:The recent development of novel repeat-fruiting types of blackberry (Rubus L.) cultivars, combined with a long history of morphological marker-assisted selection for thornlessness by blackberry breeders, has given rise to increased interest in using molecular markers to facilitate blackberry breeding. Yet no...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-8-69

    authors: Lewers KS,Saski CA,Cuthbertson BJ,Henry DC,Staton ME,Main DS,Dhanaraj AL,Rowland LJ,Tomkins JP

    更新日期:2008-06-20 00:00:00

  • Auxin mediates the touch-induced mechanical stimulation of adventitious root formation under windy conditions in Brachypodium distachyon.

    abstract:BACKGROUND:It is widely perceived that mechanical or thigmomorphogenic stimuli, such as rubbing and bending by passing animals, wind, raindrop, and flooding, broadly influence plant growth and developmental patterning. In particular, wind-driven mechanical stimulation is known to induce the incidence of radial expansio...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-02544-8

    authors: Nam BE,Park YJ,Gil KE,Kim JH,Kim JG,Park CM

    更新日期:2020-07-16 00:00:00

  • Full-length transcriptome analysis of Phytolacca americana and its congener P. icosandra and gene expression normalization in three Phytolaccaceae species.

    abstract:BACKGROUND:Phytolaccaceae species in China are not only ornamental plants but also perennial herbs that are closely related to human health. However, both large-scale full-length cDNA sequencing and reference gene validation of Phytolaccaceae members are still lacking. Therefore, single-molecule real-time sequencing te...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-02608-9

    authors: Liu D,Chen L,Chen C,An X,Zhang Y,Wang Y,Li Q

    更新日期:2020-08-27 00:00:00

  • Calmodulin-dependent and calmodulin-independent glutamate decarboxylases in apple fruit.

    abstract:BACKGROUND:The ubiquitous, non-proteinaceous amino acid GABA (γ-aminobutyrate) accumulates in plants subjected to abiotic stresses such as chilling, O2 deficiency and elevated CO2. Recent evidence indicates that controlled atmosphere storage causes the accumulation of GABA in apple (Malus x domestica Borkh.) fruit, and...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-13-144

    authors: Trobacher CP,Zarei A,Liu J,Clark SM,Bozzo GG,Shelp BJ

    更新日期:2013-09-28 00:00:00

  • microRNA-dependent gene regulatory networks in maize leaf senescence.

    abstract:BACKGROUND:Maize grain yield depends mainly on the photosynthetic efficiency of functional leaves, which is controlled by an array of gene networks and other factors, including environmental conditions. MicroRNAs (miRNAs) are small RNA molecules that play important roles in plant developmental regulation. A few senesce...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-016-0755-y

    authors: Wu X,Ding D,Shi C,Xue Y,Zhang Z,Tang G,Tang J

    更新日期:2016-03-22 00:00:00

  • Salt stress vs. salt shock - the case of sugar beet and its halophytic ancestor.

    abstract:BACKGROUND:Sugar beet is a highly salt-tolerant crop. However, its ability to withstand high salinity is reduced compared to sea beet, a wild ancestor of all beet crops. The aim of this study was to investigate transcriptional patterns associated with physiological, cytological and biochemical mechanisms involved in sa...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-019-1661-x

    authors: Skorupa M,Gołębiewski M,Kurnik K,Niedojadło J,Kęsy J,Klamkowski K,Wójcik K,Treder W,Tretyn A,Tyburski J

    更新日期:2019-02-06 00:00:00

  • Mapping QTLs and association of differentially expressed gene transcripts for multiple agronomic traits under different nitrogen levels in sorghum.

    abstract:BACKGROUND:Sorghum is an important C4 crop which relies on applied Nitrogen fertilizers (N) for optimal yields, of which substantial amounts are lost into the atmosphere. Understanding the genetic variation of sorghum in response to limited nitrogen supply is important for elucidating the underlying genetic mechanisms ...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-015-0696-x

    authors: Gelli M,Mitchell SE,Liu K,Clemente TE,Weeks DP,Zhang C,Holding DR,Dweikat IM

    更新日期:2016-01-13 00:00:00

  • Transcriptome analysis reveals insight into molecular hydrogen-induced cadmium tolerance in alfalfa: the prominent role of sulfur and (homo)glutathione metabolism.

    abstract:BACKGROUND:Hydrogen gas (H2) is hypothesised to play a role in plants that are coping with stresses by regulating signal transduction and gene expression. Although the beneficial role of H2 in plant tolerance to cadmium (Cd) has been investigated previously, the corresponding mechanism has not been elucidated. In this ...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-2272-2

    authors: Cui W,Yao P,Pan J,Dai C,Cao H,Chen Z,Zhang S,Xu S,Shen W

    更新日期:2020-02-04 00:00:00

  • An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar.

    abstract:BACKGROUND:Forest trees have important economic and ecological value. As a model tree, poplar has played a significant role in elucidating the molecular mechanisms underlying tree biology. However, a lack of mutant libraries and time-consuming stable genetic transformation processes severely limit progress into the fun...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-021-02833-w

    authors: Zheng L,Yang J,Chen Y,Ding L,Wei J,Wang H

    更新日期:2021-01-21 00:00:00

  • Genome-wide analysis of cotton GH3 subfamily II reveals functional divergence in fiber development, hormone response and plant architecture.

    abstract:BACKGROUND:Auxin-induced genes regulate many aspects of plant growth and development. The Gretchen Hagen 3 (GH3) gene family, one of three major early auxin-responsive families, is ubiquitous in the plant kingdom and its members function as regulators in modulating hormonal homeostasis, and stress adaptations. Specific...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-018-1545-5

    authors: Yu D,Qanmber G,Lu L,Wang L,Li J,Yang Z,Liu Z,Li Y,Chen Q,Mendu V,Li F,Yang Z

    更新日期:2018-12-12 00:00:00

  • Characterization of singlet oxygen-accumulating mutants isolated in a screen for altered oxidative stress response in Chlamydomonas reinhardtii.

    abstract:BACKGROUND:When photosynthetic organisms are exposed to harsh environmental conditions such as high light intensities or cold stress, the production of reactive oxygen species like singlet oxygen is stimulated in the chloroplast. In Chlamydomonas reinhardtii singlet oxygen was shown to act as a specific signal inducing...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-10-279

    authors: Fischer BB,Eggen RI,Niyogi KK

    更新日期:2010-12-17 00:00:00

  • Genomic regions associated with stripe rust resistance against the Egyptian race revealed by genome-wide association study.

    abstract:BACKGROUND:Wheat stripe rust (caused by Puccinia striiformis f. sp. Tritici), is a major disease that causes huge yield damage. New pathogen races appeared in the last few years and caused a broke down in the resistant genotypes. In Egypt, some of the resistant genotypes began to be susceptible to stripe rust in recent...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-02813-6

    authors: Abou-Zeid MA,Mourad AMI

    更新日期:2021-01-14 00:00:00

  • Synthesis of chlorophyll b: localization of chlorophyllide a oxygenase and discovery of a stable radical in the catalytic subunit.

    abstract:BACKGROUND:Assembly of stable light-harvesting complexes (LHCs) in the chloroplast of green algae and plants requires synthesis of chlorophyll (Chl) b, a reaction that involves oxygenation of the 7-methyl group of Chl a to a formyl group. This reaction uses molecular oxygen and is catalyzed by chlorophyllide a oxygenas...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-4-5

    authors: Eggink LL,LoBrutto R,Brune DC,Brusslan J,Yamasato A,Tanaka A,Hoober JK

    更新日期:2004-04-15 00:00:00

  • Impact of recurrent gene duplication on adaptation of plant genomes.

    abstract:BACKGROUND:Recurrent gene duplication and retention played an important role in angiosperm genome evolution. It has been hypothesized that these processes contribute significantly to plant adaptation but so far this hypothesis has not been tested at the genome scale. RESULTS:We studied available sequenced angiosperm g...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/1471-2229-14-151

    authors: Fischer I,Dainat J,Ranwez V,Glémin S,Dufayard JF,Chantret N

    更新日期:2014-05-31 00:00:00

  • Identification of the vernalization gene VRN-B1 responsible for heading date variation by QTL mapping using a RIL population in wheat.

    abstract:BACKGROUND:Heading time is one of the most important agronomic traits in wheat, as it largely affects both adaptation to different agro-ecological conditions and yield potential. Identification of genes underlying the regulation of wheat heading and the development of diagnostic markers could facilitate our understandi...

    journal_title:BMC plant biology

    pub_type: 杂志文章

    doi:10.1186/s12870-020-02539-5

    authors: Li Y,Xiong H,Guo H,Zhou C,Xie Y,Zhao L,Gu J,Zhao S,Ding Y,Liu L

    更新日期:2020-07-13 00:00:00