Abstract:
BACKGROUND:In mammals, nucleostemin (NS), a nucleolar GTPase, is involved in stem cell proliferation, embryogenesis and ribosome biogenesis. Arabidopsis NUCLEOSTEMIN-LIKE 1 (NSN1) has previously been shown to be essential for plant growth and development. However, the role of NSN1 in cell proliferation is largely unknown. RESULTS:Using nsn1, a loss-of-function mutant of Arabidopsis NSN1, we investigated the function of NSN1 in plant cell proliferation and cell cycle regulation. Morphologically, nsn1 exhibited developmental defects in both leaves and roots, producing severely reduced vegetative organs with a much smaller number of cells than those in the wild type. Dynamic analysis of leaf and root growth revealed a lower cell proliferation rate and slower cell division in nsn1. Consistently, the transcriptional levels of key cell cycle genes, including those regulating the transition of G1-S and G2-M, were reduced drastically in nsn1. The introduction of CYCLIN B1::GUS into nsn1 resulted in confined expression of GUS in both the leaf primordia and root meristem, indicating that cell proliferation was hampered by the mutation of NSN1. Upon subjection to treatment with bleomycin and methyl methanesulfonate (MMS), nsn1 plants exhibited hypersensitivity to the genotoxic agents. In the nucleus, NSN1 interacted with nucleosome assembly protein1 (AtNAP1;1), a highly conserved histone chaperone functioning in cell proliferation. Notably, the N-terminal conserved domains of Arabidopsis NSN1 were critical for the physical interaction. CONCLUSIONS:As a conserved homolog of mammalian nucleostemin, Arabidopsis NSN1 plays pivotal roles in embryogenesis and ribosome biogenesis. In this study, NSN1 was found to function as a positive regulator in cell cycle progression. The interaction between NSN1 and histone chaperone AtNAP1;1, and the high resemblance in sensitivity to genotoxics between nsn1 and atnap1;1 imply the indispensability of the two nuclear proteins for cell cycle regulation. This work provides an insight into the delicate control of cell proliferation through the cooperation of a GTP-binding protein with a nucleosome assembly/disassembly protein in Arabidopsis.
journal_name
BMC Plant Bioljournal_title
BMC plant biologyauthors
Wang Z,Wang X,Xie B,Hong Z,Yang Qdoi
10.1186/s12870-018-1289-2subject
Has Abstractpub_date
2018-06-01 00:00:00pages
99issue
1issn
1471-2229pii
10.1186/s12870-018-1289-2journal_volume
18pub_type
杂志文章abstract:BACKGROUND:Salt stress is one of the major abiotic stresses affecting plant growth and productivity. Vacuolar H+-pyrophosphatase (H+-PPase) genes play an important role in salt stress tolerance in multiple species. RESULTS:In this study, the promoter from the vacuolar H+-pyrophosphatase from Thellungiella halophila (T...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-90
更新日期:2010-05-18 00:00:00
abstract:BACKGROUND:Previously, transgenic trichome-bearing (hairy leaf) Brassica napus lines expressing either the Arabidopsis thaliana GL3 gene (line AtGL3+) [1] or the AtGL3 gene in combination with an RNAi construct to down-regulate TTG1 (line K-5-8) [2] were developed. The leaves of these lines exhibited altered insect fee...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1277-6
更新日期:2018-04-16 00:00:00
abstract:BACKGROUND:Drought is a major abiotic stress factors that reduces agricultural productivity. GRAS transcription factors are plant-specific proteins that play diverse roles in plant development. However, the functions of a number of GRAS genes identified in rice are unknown, especially the GRAS genes related to rice dro...
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pub_type: 杂志文章
doi:10.1186/s12870-015-0532-3
更新日期:2015-06-13 00:00:00
abstract:BACKGROUND:Eukaryotic cilia are complex, highly conserved microtubule-based organelles with a broad phylogenetic distribution. Cilia were present in the last eukaryotic common ancestor and many proteins involved in cilia function have been conserved through eukaryotic diversification. However, cilia have also been lost...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-11-185
更新日期:2011-12-30 00:00:00
abstract:BACKGROUND:The phloem of dicotyledonous plants contains specialized P-proteins (phloem proteins) that accumulate during sieve element differentiation and remain parietally associated with the cisternae of the endoplasmic reticulum in mature sieve elements. Wounding causes P-protein filaments to accumulate at the sieve ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-219
更新日期:2010-10-08 00:00:00
abstract:BACKGROUND:Mitochondria are dynamic organelles that move along actin filaments, and serve as calcium stores in plant cells. The positioning and dynamics of mitochondria depend on membrane-cytoskeleton interactions, but it is not clear whether microfilament cytoskeleton has a direct effect on mitochondrial function and ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-53
更新日期:2010-03-24 00:00:00
abstract:BACKGROUND:In order to grow, plants rely on soil nutrients which can vary both spatially and temporally depending on the environment, the soil type or the microbial activity. An essential nutrient is nitrogen, which is mainly accessible as nitrate and ammonium. Many studies have investigated transport genes for these i...
journal_title:BMC plant biology
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doi:10.1186/s12870-019-1768-0
更新日期:2019-05-20 00:00:00
abstract:BACKGROUND:Currently, Tectona grandis is one of the most valuable trees in the world and no transcript dataset related to secondary xylem is available. Considering how important the secondary xylem and sapwood transition from young to mature trees is, little is known about the expression differences between those succe...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-015-0599-x
更新日期:2015-09-15 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
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更新日期:2007-11-23 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
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更新日期:2016-01-05 00:00:00
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journal_title:BMC plant biology
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doi:10.1186/s12870-019-2058-6
更新日期:2019-10-22 00:00:00
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pub_type: 杂志文章,收录出版
doi:10.1186/s12870-019-2090-6
更新日期:2019-12-04 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
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更新日期:2016-01-19 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1518-8
更新日期:2018-11-20 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-9-65
更新日期:2009-05-29 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-144
更新日期:2013-09-28 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-14-124
更新日期:2014-05-08 00:00:00
abstract:BACKGROUND:The green peach aphid (GPA), Myzus persicae, is economically one of the most threatening pests in pepper cultivation, which not only causes direct damage but also transmits many viruses. Breeding aphid resistant pepper varieties is a promising and environmentally friendly method to control aphid populations ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1340-3
更新日期:2018-06-27 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-017-1152-x
更新日期:2017-11-17 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12870-015-0608-0
更新日期:2015-09-11 00:00:00
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pub_type: 杂志文章
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更新日期:2010-05-07 00:00:00
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pub_type: 杂志文章
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更新日期:2014-08-21 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
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更新日期:2012-02-16 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-11-25
更新日期:2011-01-26 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
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更新日期:2019-01-07 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
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更新日期:2018-01-04 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
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更新日期:2007-07-31 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
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更新日期:2015-02-13 00:00:00
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journal_title:BMC plant biology
pub_type: 杂志文章
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更新日期:2018-02-15 00:00:00
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pub_type: 杂志文章
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更新日期:2020-05-24 00:00:00