Abstract：
BACKGROUND:To study the efficacy of cisplatin-loaded M1 macrophage secreted-exosomes (DDP-M1-Exos) in enhancing DDP anti-tumor effect. METHODS:M1-Exos were first extracted using density gradient centrifugation, and the DDP-M1-Exos system was established via electroporation. Then, the morphology, particle size and maker proteins of the DDP-M1-Exos system were assessed using transmission electron microscope (TEM), DLS and Western blotting, respectively. The uptake of Exos by mouse Lewis lung cancer (LLC) cells was observed under a confocal laser scanning microscope, and the influence of the DDP-M1-Exos system on the viability of Lewis cells was determined using methyl thiazolyl tetrazolium (MTT) assay and 4',6-diamidino-2-phenylindole (DAPI) staining. Besides, its impacts on the messenger ribonucleic acid (mRNA) levels and protein expression levels of B-cell lymphoma 2 (Bcl-2), Bcl-2 associated X protein (Bax) and Caspase-3 in LLC cells were examined using quantitative real-time PCR (qRT-PCR) and Western blotting(WB), respectively. Finally, with the LLC-bearing mouse model as the object of study, the efficacy was evaluated using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and hematoxylin-eosin staining (HE) staining in vivo. RESULTS:Saucer-shaped Exos with a bilayer membrane, uniform particle size and highly expressed CD81, Alix and HSP70 were successfully isolated from M1 macrophages via density gradient centrifugation, and they were absorbed by LLC cells. Meanwhile, the DDP-M1-Exos drug delivery system was successfully prepared using electroporation technique. Compared with Control group, DDP and DDP-M1-Exos groups exhibited a decrease in the proliferation rate of mouse LLC cells and an increase in their apoptosis rate (*P<0.05), and the apoptosis rate in DDP-M1-Exos group was substantially higher than that in DDP group (#P<0.01). According to the qRT-PCR and WB results, DDP-M1-Exos up-regulated Bax and Caspase-3 in the apoptosis signaling pathway to induce tumor cell apoptosis, thereby resisting tumors. Moreover, through in vivo experiments, it was found that M1-Exos alone had a potential to suppress tumor growth, and that the carrier of M1-Exos could not only kill tumor cells, but also encapsulate DDP to enhance its anti-lung cancer effect. CONCLUSIONS:The DDP-M1-Exos drug delivery system is prepared using Exos extracted via density gradient centrifugation by electroporation, and the drug-loaded Exos are able to effectively inhibit the proliferation of tumor cells and induce their apoptosis, exerting an anti-tumor effect.
journal_name
Minerva Medjournal_title
Minerva medicaauthors
Li J,Li N,Wang Jdoi
10.23736/S0026-4806.20.06564-7subject
Has Abstractpub_date
2020-04-08 00:00:00eissn
0026-4806issn
1827-1669pii
S0026-4806.20.06564-7pub_type
杂志文章相关文献
MINERVA MEDICA文献大全abstract：:After examining the most recent findings on the physiopathology of the renin-angiotensin system, techniques are analysed for the determination of renin activity (PRA). These are still inadequate and imprecise, notwithstanding radioimmunological techniques and stress is laid on the importance of their standardisation. ...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：1980-09-08 00:00:00
abstract：:The thyroid activity of twenty euthyroid patients, with single or multiple "cold" nodules was investigated, with determination of seric T3, T4, reverse-T3 (rT3) and TSH. The blood-drags were performed respectively, during (with seric samples from the inferior thyroid vein ipsilateral to the affected lobe) and after (1...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1986-05-19 00:00:00
abstract：:The object of the present study is to review all that in the last years has been discovered about growth hormone-releasing hormone (GHRH), in order to point out both its physiopathological characteristics and its possible diagnostic and therapeutic use. In the first section are summarily reviewed the different studies...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：1989-04-01 00:00:00
abstract：:Changes in body weight (absolute and percent), overweight index, and body surface area were examined in 27 grossly obese subjects after jejunal-ileal by-pass. Curves were steeper and linear for 3-4 months and then flattened out for the remainder of the twelve-month period of observation. They were parallel to each oth...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1978-11-17 00:00:00
abstract：:Penbutolol has proved particularly effective and suitable for the treatment, even on a long-term basis, of recently developed hypertension, especially in its hyperkinetic forms. The drug produces minimal side effects, is well tolerated and gives an early therapeutic response. In addition penbutolol does not cause any ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1987-09-30 00:00:00
abstract：AIM:The aim of this supplement registry was to evaluate the efficacy of the Pycnogenol® in improving cochlear flow and symptoms in a 6-month follow-up for patients with Meniere's disease (MD), tinnitus and cochlear hypoperfusion. METHODS:Main signs/symptoms were considered: Spontaneous vertigo, positional vertigo, hea...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：2014-06-01 00:00:00
abstract：BACKGROUND:MALAT1 (Metastasis-associated lung adenocarcinoma transcript 1), a newly discovered long non-coding RNA (lncRNA), has been reported to be overexpressed in various cancers. However, the clinical value of MALAT1 in digestive system cancers is unclear. This study was designed to investigate the potential value ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：2016-05-17 00:00:00
abstract：:Buckley's syndrome is a nosologic entity characterized by the presence from birth of extremely elevated levels of circulating immunoglobulin E and an important deficiency of polymorphonuclear chemotaxis. The consequences of such alterations are represented by the premature rising of recurrent cutaneous, pulmonary and ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：BACKGROUND:Several studies demonstrate that the adherence to asthma guidelines (GL) is poor, but only a few of them were performed in community pharmacies. Thus, we decided to study this phenomenon by administering a questionnaire (Q) in two pharmacies. METHODS:A Q was developed and administered to 138 patients-custom...
journal_title：Minerva medica
pub_type： 杂志文章,多中心研究
doi：
更新日期：2003-02-01 00:00:00
abstract：:Merkel cell carcinoma (MCC) is a rare malignant cutaneous neuroendocrine tumour with an aggressive behaviour and frequent regional lymph node and distant metastases. It mostly occurs in old patients and the commonest sites are the skin of the head, neck and the extremities. Typically, the primary tumour presents as a ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：2008-06-01 00:00:00
abstract：:The Authors report a case of chylous mesenteric cyst of great size in an adult patient. They stress the diagnostic value of roentgen and sonographic findings that are determinant for a correct preoperative diagnosis. Sonography is the most important diagnostic tool; it leads to identification and localization of the m...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1982-12-15 00:00:00
abstract：:In the present paper the incidence of lymphomatous disease in Messina and its province, with growing urbanization and rural limiting areas, is discussed: by analysing 150 cases of malignant lymphoma observed at our Institute from 1990 to 1995. The method proposed, based on data obtained from the medical files of these...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：1999-05-01 00:00:00
abstract：:A simple routine endoscopic screening test has been sought for the diagnosis of chronic atrophic gastritis. An endoscopic-bioptic study was therefore carried out on 850 subjects presenting consecutively at a Digestive Endoscopy Department with dyspeptic-pain symptomatology. In a first sample of 389 patients, 2 biopsie...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1989-09-01 00:00:00
abstract：:Fifty-one alcoholics, previously admitted to the hospital for medical care and treated with disulfiram, were re-examinated after 1-3 years. In thirty of them (58%) total abstinence was found; eighteen patients (35%) behaved so in spite of disulfiram withhold during the first year of self-administration. No side-effect...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1979-04-28 00:00:00
abstract：:The behaviour of urinary NAG activity was studied in patients during menopause, some of whom were given estrogen therapy due to severe vasomotor symptoms (hot flushes etc.). One group of these patients revealed slightly higher than normal blood pressure. A second group had slight arterial hypertension and pancreatic d...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1987-03-31 00:00:00
abstract：:The Authors report the findings of a perspective study carried out in 214 cases of acute diarrhoea to estimate the presence of giardia intestinalis infections. The incidence of 3.2% has been discussed on the bases of recent epidemiological advances. ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:A case of carcinoid tumor of the last ileal loop is presented; the first clinical sign was exophthalmos due to bilateral orbital metastases. The case is believed to be the third recorded one of carcinoid with metastases to the orbit; other metastatic lesions were located in myocardium, liver, spleen, kidney, ovary and...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1979-03-17 00:00:00
abstract：:A case of aneurysmatic dilatation of the vena cava superior is presented. This lesion is rare and not commonly taken into consideration in the differential diagnosis of anterior mediastinal masses. Chest X-rays taken during inspiration and expiration and in the standing and reclining positions (particularly the second...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1978-10-27 00:00:00
abstract：:Relaxation and tranquility are commonly induced by acupuncture. Techniques are presented and neurophysiological theories underlying the therapeutic effect are discussed. Emphasis is laid on the importance of this effect in psychiatric management. ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1976-06-09 00:00:00
abstract：:Diclofenac is a non-steroidal antinflammatory drug (NSAID) that finds indication in the treatment of debilitating pathologies characterized by chronic pain sustained by inflammation, such as in rheumatic disease (rheumatoid arthritis or osteoarthritis) or periarthritis, bursitis, tendonitis, myositis and sciatica. Its...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：2016-06-01 00:00:00
abstract：:The combination acetominophen/tramadol implies the use of two molecules which are different in the pharmacological structure but share a biological similarity in kinetics. Their respective actions are directed to different components of the nociceptive afference. Hematological clinical practice daily challenges diseas...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：2008-12-01 00:00:00
abstract：:The subject of this dissertation is the diet of flight personnel. A rapid review of the general question of diet in general and that of flight personnel in particular is given. On this basis, the various aspects of the problem (energy sources, calorie requirements, activity of the individual, etc.) are examined. The c...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1982-08-25 00:00:00
abstract：:0.3 mg/day betamethyldigoxin was given per os in three daily administrations to 8 healthy subjects, and 8 compensated and 8 decompensated heart patients. Prior to the treatment, and 6 hr after the last administration, blood digoxin values were determined radio-immunologically, together with cardiac output, systolic st...
journal_title：Minerva medica
pub_type： 杂志文章,评审
doi：
更新日期：1981-02-18 00:00:00
abstract：:The authors report a case of pheochromocytoma revealed by a de novo appeared hypertension in a young female patient at her 3rd trimester of pregnancy. An early diagnosis and an appropriate medical management allowed a successful maternal outcome. Since maternal and fetal prognosis of pheochromocytoma associated with p...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1981-05-07 00:00:00
abstract：:Penile systolic pressure has been evaluated by the Doppler-sonographic method in 15 diabetic patients suffering from erectile impotence. Urodynamic tests have also been carried out in some patients for a diagnosis of neurological impotence. The results obtained show the possibility of a double or sole origin (neurogen...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1981-12-08 00:00:00
abstract：:Cytology using fine needle aspiration biopsy has been widely used in dysplastic and neoplastic thyroid lesions as it is free of complications and very accurate. Personal experience with 2212 thyroid fine needle aspiration biopsies (1564 patients) performed in the period 1983-1987 is reported. Cytologic and histologic ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:Certain applications of radioisotopes to obstetric and gynaecological diagnosis are illustrated. Radioisotopes have been widely employed since the improvement in instrumental methods and the availability of short-life radioisotopes has removed the basic limitation which once prevented their use during the entire fecun...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1975-12-12 00:00:00
abstract：:A case of myelodysplasia (refractory anaemia with excessive blasts) arising 7 years after a 3 year period of intermittent monthly treatment cycles with melphalan for multiple myelomas is reported. This is another example of preleukaemic syndrome possibly caused by melphalan. Among the possible pathogenetic mechanisms,...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1983-07-14 00:00:00
abstract：:A case of serious, recurrent enterorrhagia is reported. The cause could not be identified with normal diagnostic tools and was discovered only after explorative laparotomy. The finding was benign ulcerated tumour of the small intestine, histologically defined as fibroma with fluorishing cellularity. ...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1976-05-05 00:00:00
abstract：:The authors describe one case of infections sacro-iliitis caused by Borrelia, where the diagnostic procedures by conventional radiology, computerized tomography and nuclear magnetic resonance imaging have not permitted the localization and the assessment of joint inflammatory disease. The bone "theree phases" scintisc...
journal_title：Minerva medica
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
