Abstract：
OBJECTIVES:Mechanobiology phenomena constitute a major element of the cellular and tissue response during orthodontic treatment and the implantation of a biomaterial. Better understanding these phenomena will improve the effectiveness of our treatments. The objective of this work is to validate a model of three-dimensional (3D) culture of osteoblasts to study mechanobiology. MATERIALS AND METHODS:The hFOB 1.19 cell line was cultured either traditionally on a flat surface or in aggregates called spheroids. They were embedded in 0.8% low-melting agarose type VII and placed in a polyethylene terephthalate transwell insert. Compressive forces of 1 and 4 g/cm2 were applied with an adjustable weight. Proliferation was evaluated by measuring diameters, monitoring glucose levels, and conducting Hoechst/propidium iodide staining. Enzyme-linked immunosorbent assays focusing on the pro-inflammatory mediators interleukin (IL)-6 and IL-8 and bone remodelling factor osteoprotegerin were performed to evaluate soluble factor synthesis. quantitative reverse transcription-polymerase chain reaction was performed to evaluate bone marker transcription. RESULTS:The 3D model shows good cell viability and permits IL dosing. Additionally, three gene expression profiles are analysable. LIMITATIONS:The model allows analysis of conventional markers; larger exploration is needed for better understanding osteoblast mechanobiology. However, it only allows an analysis over 3 days. CONCLUSION:The results obtained by applying constant compressive forces to 3D osteoblastic cultures validate this model system for exploring biomolecule release and analysing gene transcription. In particular, it highlights a disturbance in the expression of markers of osteogenesis.
journal_name
Eur J Orthodjournal_title
European journal of orthodonticsauthors
Brezulier D,Pellen-Mussi P,Tricot-Doleux S,Novella A,Sorel O,Jeanne Sdoi
10.1093/ejo/cjaa017subject
Has Abstractpub_date
2020-09-11 00:00:00pages
387-395issue
4eissn
0141-5387issn
1460-2210pii
5794375journal_volume
42pub_type
杂志文章abstract：:The aim of this study was to determine whether Fluor Protector, a fluoride varnish, applied to molars before orthodontic banding could prevent white spot formation. In the in vitro study 93 human premolars were used, divided in five different groups, representing different clinical situations. Each tooth was sliced in...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/12.3.316
更新日期：1990-08-01 00:00:00
abstract：OBJECTIVE:To investigate the factors associated with the change in alveolar bone level of mandibular second and third molars after second molar protraction into the space of the missing first molar (L6) or second premolar (LE). METHODS:Fifty-one patients in whom space of the missing L6 or LE was treated with second mo...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjz001
更新日期：2019-09-21 00:00:00
abstract：Background/objective:The aim of this study was to evaluate the efficacy of tooth alignment with conventional and self-ligating labial and lingual orthodontic bracket systems. Materials/methods:We tested labial brackets (0.022″ slot size) and lingual brackets (0.018″ slot size). The labial brackets were: (i) regular tw...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjy005
更新日期：2018-11-30 00:00:00
abstract：:The aim of this research was to evaluate the effect of the Delaire surgical technique on the midfacial morphology in a group of subjects with a congenital unilateral cleft of lip and palate (UCLP), prior to orthodontic treatment. Thirty-five UCLP (15 left and 20 right) patients (16 males and 19 females, mean age 7.03+...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjn068
更新日期：2009-02-01 00:00:00
abstract：BACKGROUND:Although ceramic brackets have been extensively used for decades in orthodontics there is not till today any study focusing on the possible deterioration of mechanical properties after in vivo ageing. OBJECTIVES:To determine whether the mechanical properties of alumina orthodontic brackets change after intr...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjz024
更新日期：2020-01-27 00:00:00
abstract：:The purpose of this investigation was to assess the vertical changes occurring in skeletal open bite patients treated orthodontically with different extraction patterns. The study was conducted using lateral cephalometric radiographs taken before and after treatment. Fifteen patients who had an anterior open bite (AOB...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/24.4.407
更新日期：2002-08-01 00:00:00
abstract：Objective:To investigate effects of mandible advanced device (MAD) therapy for obstructive sleep apnoea-hypopnea syndrome (OSAHS) on the neuron apoptosis and acetylcholine esterase activity in frontal cortex. Materials and methods:Thirty male New Zealand white rabbits were randomly divided into three groups (n = 10 in...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjx060
更新日期：2018-05-25 00:00:00
abstract：:This study was undertaken to assess the factors affecting self-perception and the demand for orthodontic treatment among north Jordanian school children. In total, 1404 students randomly selected to represent five geographical areas of northern Jordan were examined. The students were further subgrouped according to ge...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjh094
更新日期：2005-06-01 00:00:00
abstract：Objective:To evaluate the transverse relationships of the first permanent molars after the correction of posterior crossbite performed during the deciduous dentition with two different treatment protocols. Materials/methods:Ninety patients (40 males and 50 females) with posterior crossbite were treated during the deci...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjw080
更新日期：2017-10-01 00:00:00
abstract：:This paper describes and illustrates an innovative versatile skull-holding device for experimental research in cephalometrics. Repositioning of the skulls was evaluated and found to be highly reproducible. ...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/18.2.193
更新日期：1996-04-01 00:00:00
abstract：:The analysis of the non-linear and time-dependent viscoelasticity of the periodontal ligament (PDL) enables a better understanding of the biomechanical features of the key regulator tissue for tooth movement. This is of great significance in the field of orthodontics as targeted tooth movement remains still one of the...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjs103
更新日期：2013-12-01 00:00:00
abstract：Objective:To assess the dental, skeletal, and profile changes after functional appliances and subsequent fixed appliances treatment in order to quantify their effect and evaluate the influence of initial incisal inclination and growth pattern. Materials and methods:A retrospective cephalometric analysis was performed ...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjw064
更新日期：2017-06-01 00:00:00
abstract：:Cervical column morphology was examined in adult patients with obstructive sleep apnoea (OSA) and compared with the cervical morphology of an adult control group with neutral occlusion, normal craniofacial morphology, and no history of sleep apnoea. The sleep apnoea group consisted of 91 patients, 16 females aged 29-5...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjn028
更新日期：2008-10-01 00:00:00
abstract：:The aim of this investigation was to compare the traditional method of manual cephalometric tracing with four different computerized tracing programs, where the lateral cephalograms were scanned at 300 dpi and digitized onscreen. Thirty randomly selected cephalometric radiographs were used in this study. Four programs...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjr008
更新日期：2012-06-01 00:00:00
abstract：:The publication of the three-paper series on the effects of two slot sizes on treatment parameters in this issue, targets a topic, which has attracted a lot of discussion based primarily on anecdotal evidence. The conclusive evidence contribute to the lack of variation of treatment variables with slot size selection, ...
journal_title：European journal of orthodontics
pub_type： 评论,杂志文章
doi：10.1093/ejo/cjy051
更新日期：2019-05-24 00:00:00
abstract：:Two-hundred-and-thirty-eight subjects in three different age-groups (7, 11, and 15 years) were followed over a period of 4-5 years in respect of morphological malocclusions, and signs and symptoms of functional disturbances. About half of the 7-year-olds had at least one of the morphological malocclusions registered w...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/12.4.399
更新日期：1990-11-01 00:00:00
abstract：:Orthodontic torque expression is the result of axial rotation of rectangular archwires within a rectangular bracket slot. This study investigates the effect of bracket material on torque expression. Torque exerted by a rotating archwire on each bracket will be measured as well as the relative deformation of each brack...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjr120
更新日期：2014-08-01 00:00:00
abstract：:The aim of this study was to investigate potential occurrence of bacteremia in orthodontic patients after removal of miniscrews.The study group comprised 30 healthy subjects (17 males, 13 females) with a mean age of 24.1 years treated with self-ligating fixed appliances and mini-implant anchorage. Two 20 ml venous blo...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjs099
更新日期：2014-02-01 00:00:00
abstract：AIM:Compare surface roughness of 'aesthetic' nickel-titanium (NiTi) archwires with their dynamic frictional properties. METHODS:Archwires investigated were: four fully coated tooth coloured [Forestadent: Biocosmetic (FB) and Titanol Cosmetic (FT); TOC Tooth Tone (TT); and Hawley Russell Coated Superelastic NiTi (HRC)]...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cju004
更新日期：2015-02-01 00:00:00
abstract：Background:Detection of pubertal growth peak is vital in orthodontic treatment timing and planning. Gingival crevicular fluid (GCF) contains abundant proteins from different sources and is an ideal source of biomarkers. The aim of this research is to detect candidate GCF biomarkers of pubertal growth by tandem mass tag...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjx082
更新日期：2018-07-27 00:00:00
abstract：OBJECTIVES:To examine the relationships between dental appearance, characteristics of the individual and their environment, and oral health-related quality of life (OHQoL) in young people over time. METHODS:A total of 374 young people (122 boys, 252 girls) aged 11-12 years from seven different XX schools were recruite...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cju076
更新日期：2015-10-01 00:00:00
abstract：:The aim of this presentation is to describe an innovative adhesive procedure for connecting palatal implants with transpalatal arches (TPAs). The steps required for completing the procedure, the costs involved and the requisite time were reviewed and compared with those of two alternative procedures reported in the li...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cji002
更新日期：2005-06-01 00:00:00
abstract：:The purpose of this study was to investigate cephalometrically, the effects of a functional appliance-headgear combination, the Elastic Bite-block (EBB). Of 50 Class II division 1 subjects who participated in this study, 30 made up the treatment group and 20 the control group. All of the selected cases exhibited mandi...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/17.5.375
更新日期：1995-10-01 00:00:00
abstract：:The surface topography of 100 clinically used archwires of stainless steel, beta-, or nickel-titanium were investigated that had contacted either ceramic or stainless steel brackets. One group consisted of two sets: 60 wires with no treatment records accessed to bias analyses, and 40 wires for which extensive clinical...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/22.4.409
更新日期：2000-08-01 00:00:00
abstract：:The aims of this study were to evaluate the frequency of errors in panoramic radiographs in young orthodontic patients, to register pathologic and abnormal conditions, and to compare these findings with the patient's record. A total of 1287 panoramic radiographs of children and adolescents (530 boys and 757 girls; mea...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjr035
更新日期：2012-08-01 00:00:00
abstract：:The aim of this study was to statistically assess the morphological changes of the rat periodontal ligament (PDL) and its vascularity in relation to varied magnitudes of superelastic force in experimental tooth movement using nickel-titanium (NiTi) alloy wire. Forces of 0.8, 1.6, 4, 8, and 18 g were applied to the upp...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjn075
更新日期：2009-02-01 00:00:00
abstract：:The aim of the present study was to examine dentoalveolar development in subjects with an 'ideal' (normal) occlusion. The material comprised 436 study casts of 189 male and 247 female subjects of Swedish origin between the ages of 5 and 31 years with no history of orthodontic treatment. Tooth width, and arch length, w...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjn124
更新日期：2009-04-01 00:00:00
abstract：:The purpose of this study was to determine whether an additional application of Fluor Protector before band cementation with glass ionomer cement reduces white spot formation compared with band cementation with glass ionomer cement. In the in vitro study, 80 premolars were divided in half, creating a control and a tes...
journal_title：European journal of orthodontics
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1093/ejo/20.3.219
更新日期：1998-06-01 00:00:00
abstract：Background/objective:To assess the validity and reliability of a series of three questionnaires for the quantification of patient perception of fixed appliance orthodontic treatment. Subjects and methods:The study was carried out at the University of Dundee with content and face validity being assessed using proformas...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/cjw076
更新日期：2017-08-01 00:00:00
abstract：:The aim of this study was to investigate the short- and long-term effects induced by rapid maxillary expansion (RME) on the shape of the maxillary and circummaxillary structures by means of thin-plate spline (TPS) analysis. The sample consisted of 42 patients who were compared with a control sample of 20 subjects. The...
journal_title：European journal of orthodontics
pub_type： 杂志文章
doi：10.1093/ejo/24.2.143
更新日期：2002-04-01 00:00:00
