Abstract:
:Despite the introduction of novel targeted therapies, chemotherapy still remains the primary treatment for metastatic melanoma in poorly funded healthcare environments or in case of disease relapse, with no reliable molecular markers for progression-free survival (PFS) available. As chemotherapy primarily eliminates cancer cells by apoptosis, we here evaluated if the expression of key apoptosis regulators (Bax, Bak, Bcl-2, Bcl-xL, Smac, Procaspase-9, Apaf-1, Procaspase-3 and XIAP) allows prognosticating PFS in stage III/IV melanoma patients. Following antibody validation, marker expression was determined by automated and manual scoring of immunohistochemically stained tissue microarrays (TMAs) constructed from treatment-naive metastatic melanoma biopsies. Interestingly and counter-intuitively, low expression of the pro-apoptotic proteins Bax, Bak and Smac indicated better prognosis (log-rank p < 0.0001, p = 0.0301 and p = 0.0227 for automated and p = 0.0422, p = 0.0410 and p = 0.0073 for manual scoring). These findings were independently validated in the cancer genome atlas (TCGA) metastatic melanoma cohort (TCGA-SKCM) at transcript level (log-rank p = 0.0004, p = 0.0104 and p = 0.0377). Taking expression heterogeneity between the markers in individual tumour samples into account allowed defining combinatorial Bax, Bak, Smac signatures that were associated with significantly increased PFS (p = 0.0002 and p = 0.0028 at protein and transcript level, respectively). Furthermore, combined low expression of Bax, Bak and Smac allowed predicting prolonged PFS (> 12 months) on a case-by-case basis (area under the receiver operating characteristic curve (ROC AUC) = 0.79). Taken together, our results therefore suggest that Bax, Bak and Smac jointly define a signature with potential clinical utility in chemotherapy-treated metastatic melanoma.
journal_name
Cell Death Disjournal_title
Cell death & diseaseauthors
Guttà C,Rahman A,Aura C,Dynoodt P,Charles EM,Hirschenhahn E,Joseph J,Wouters J,de Chaumont C,Rafferty M,Warren M,van den Oord JJ,Gallagher WM,Rehm Mdoi
10.1038/s41419-020-2309-3subject
Has Abstractpub_date
2020-02-13 00:00:00pages
124issue
2issn
2041-4889pii
10.1038/s41419-020-2309-3journal_volume
11pub_type
杂志文章abstract::Resistin is an adipokine that is associated with obesity, inflammation, and various cancers. Chondrosarcomas are primary malignant bone tumors that have a poor prognosis. VEGF-A is a critical angiogenic factor that is known to promote angiogenesis and metastasis in chondrosarcoma. It is unknown as to whether resistin ...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-018-1241-2
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abstract::Interleukin-8 (IL-8) expression correlates with poor prognosis in many cancers, including head and neck squamous cell carcinoma (HNSCC), but the underlying mechanism is poorly understood. In this study, we found that overexpression of IL-8 correlated with poor outcome in HNSCC patients. IL-8 significantly increased ce...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-020-2627-5
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abstract::Ionizing radiation (IR) response has been extensively investigated in BMSCs with an increasing consensus that this type of cells showed relative radiosensitivity in vitro analysis. However, the underlying mechanism of IR-induced injury of BMSCs has not been elucidated. In current study, the regulatory role of miR-22/R...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-019-1373-z
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abstract::Mammalian folliculogenesis is a complex process in which primordial follicles develop into pre-ovulatory follicles, followed by ovulation to release mature oocytes. In this study, we explored the role of miR-144 in ovulation. miR-144 was one of the differentially expressed microRNAs, which showed 5.59-fold changes, in...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2017.24
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abstract::Hereditary pancreatitis (HP) is an autosomal dominant disease that displays the features of both acute and chronic pancreatitis. Mutations in human cationic trypsinogen (PRSS1) are associated with HP and have provided some insight into the pathogenesis of pancreatitis, but mechanisms responsible for the initiation of ...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2014.120
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abstract::We previously showed that injury by partial duct ligation (PDL) in adult mouse pancreas activates Neurogenin 3 (Ngn3)(+) progenitor cells that can differentiate to β cells ex vivo. Here we evaluate the role of Ngn3(+) cells in β cell expansion in situ. PDL not only induced doubling of the β cell volume but also increa...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2013.52
更新日期:2013-03-07 00:00:00
abstract::Phosphatase and tensin homolog (PTEN) regulates apoptosis and axonal growth in the developing and adult central nervous system (CNS). Here, we show that human PTEN C-terminal PDZ interactions play a critical role in neuronal apoptosis and axon regeneration after traumatic CNS injury and stroke, highlighted by the find...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-019-1802-z
更新日期:2019-07-29 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Cell death & disease
pub_type: 已发布勘误
doi:10.1038/s41419-019-1722-y
更新日期:2019-06-17 00:00:00
abstract::Histamine is a widely distributed biogenic amine involved in the regulation of an array of biological processes. Serum histamine level is markedly elevated in the early stages of acute myocardial infarction, whereas the role it plays remains unclear. Histidine decarboxylase (HDC) is the unique enzyme responsible for h...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-018-0723-6
更新日期:2018-06-07 00:00:00
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journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2016.277
更新日期:2016-09-29 00:00:00
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journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-020-2595-9
更新日期:2020-05-21 00:00:00
abstract::The WWOX tumor suppressor is a WW domain-containing protein. Its function in the cell has been shown to be mediated, in part, by interacting with its partners through its first WW (WW1) domain. Here, we demonstrated that WWOX via WW1 domain interacts with p53 homolog, ΔNp63α. This protein-protein interaction stabilize...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2013.6
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abstract::Glycogen synthase kinase-3β (GSK-3β) has emerged as a critical factor in several pathways involved in hippocampal neuronal maintenance and function. In Huntington's disease (HD), there are early hippocampal deficits both in patients and transgenic mouse models, which prompted us to investigate whether disease-specific...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2016.104
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abstract::BMP2 expression is spatiotemporally correlated with embryo implantation and is crucial for endometrial decidualization and fertility in mice. BMP2 has been reported to increase the mesenchymal adhesion molecule N-cadherin and enhance cell invasion in cancer cells; moreover, studies suggest that N-cadherin promotes pla...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-017-0230-1
更新日期:2018-02-07 00:00:00
abstract::Menin, encoded by the multiple endocrine neoplasia type 1 (MEN1) gene, is a tumor suppressor that leads to multiple endocrine tumors upon loss of its function. Menin functions as a transcriptional activator by tethering MLL complex to mediate histone H3 K4 methylation. It also functions as a repressor. However, the mo...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2013.98
更新日期:2013-04-11 00:00:00
abstract::Glioma patients commonly suffer from epileptic seizures. However, the mechanisms of glioma-associated epilepsy are far to be completely understood. Using glioma-neurons co-cultures, we found that tumor cells are able to deeply influence neuronal chloride homeostasis, by depolarizing the reversal potential of γ-aminobu...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2014.437
更新日期:2014-10-30 00:00:00
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journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-020-02836-9
更新日期:2020-08-09 00:00:00
abstract::Inactivation of the retinoblastoma protein (Rb) has a key role in tumorigenesis. It is well established that Rb function is largely regulated by a dynamic balance of phosphorylation and dephosphorylation. Although much research has been done to understand the mechanisms and function of RB phosphorylation, the regulati...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2015.3
更新日期:2015-02-12 00:00:00
abstract::Adipogenesis is a process which induces or represses many genes in a way to drive irreversible changes of cell phenotypes; lipid accumulation, round cell-shape, secreting many adipokines. As a master transcription factor (TF), PPARγ2 induces several target genes to orchestrate these adipogenic changes. Thus induction ...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-018-1249-7
更新日期:2019-01-08 00:00:00
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journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-018-1228-z
更新日期:2018-12-05 00:00:00
abstract::Glioblastoma multiforme (GBM) are resistant to TNFα-induced apoptosis and blockade of TNFα-induced NF-κB activation sensitizes glioma cells to apoptosis. As Casein kinase-2 (CK2) induces aberrant NF-κB activation and as we observed elevated CK2 levels in GBM tumors, we investigated the potential of CK2 inhibitors (CK2...
journal_title:Cell death & disease
pub_type: 杂志文章
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journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2010.67
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journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2014.257
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journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/cddis.2012.195
更新日期:2013-01-10 00:00:00
abstract::Neonatal jaundice is prevalent among newborns and can lead to severe neurological deficits, particularly sensorimotor dysfunction. Previous studies have shown that bilirubin (BIL) enhances the intrinsic excitability of central neurons and this can potentially contribute to their overexcitation, Ca2+ overload, and neur...
journal_title:Cell death & disease
pub_type: 杂志文章
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journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-018-1032-9
更新日期:2018-10-03 00:00:00
abstract::As an important modality for the local control of colorectal cancer (CRC), radiotherapy or neoadjuvant radiotherapy is widely applied in the clinic, but radioresistance has become a major obstacle for CRC radiotherapy. Here we reported that B7-H3, an important costimulatory molecule, is associated with radioresistance...
journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-020-03041-4
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journal_title:Cell death & disease
pub_type: 杂志文章
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更新日期:2017-04-06 00:00:00
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journal_title:Cell death & disease
pub_type: 杂志文章
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journal_title:Cell death & disease
pub_type: 杂志文章
doi:10.1038/s41419-017-0133-1
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