Abstract：
:Estimates of quantitative proteomic distance between populations have not been reported to date. Here, quantitative proteomic distances between three Spanish bovine breeds (Asturiana de los Valles, AV; Retinta, RE; and Rubia Gallega, RG) were estimated from two-dimensional electrophoresis profiles of meat samples of longissimus thoracis muscle at 2 h post-mortem. Statistically significant distances were detected between AV/RG and the most genetically different RE breed, using the novel QD measure of quantitative proteomic distance. In total, 18 differentially abundant myofibrillar and sarcoplasmic proteins/isoforms contributing to proteomic distances between breeds were confidently identified by tandem mass spectrometry. The fast skeletal myosin regulatory light chain 2 followed by other five interacting proteins exhibited the most pronounced relative change between breeds. In addition, most differentially represented proteins could be associated with variations in meat tenderness. Therefore, they could be candidate biomarkers for molecular breeding programs and authentication of the three Spanish beef breeds.
journal_name
Food Chemjournal_title
Food chemistryauthors
Rodríguez-Vázquez R,Mato A,López-Pedrouso M,Franco D,Sentandreu MA,Zapata Cdoi
10.1016/j.foodchem.2020.126293subject
Has Abstractpub_date
2020-06-15 00:00:00pages
126293eissn
0308-8146issn
1873-7072pii
S0308-8146(20)30142-4journal_volume
315pub_type
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