Abstract:
BACKGROUND:Meloidogyne chitwoodi commonly known as Columbia root-knot nematode or CRKN is one of the most devastating pests of potato in the Pacific Northwest of the United States of America. In addition to the roots, it infects potato tubers causing internal as well as external defects, thereby reducing the market value of the crop. Commercial potato varieties with CRKN resistance are currently unavailable. Race specific resistance to CRKN has been introgressed from the wild, diploid potato species Solanum bulbocastanum into the tetraploid advanced selection PA99N82-4 but there is limited knowledge about the nature of its resistance mechanism. In the present study, we performed histological and differential gene expression profiling to understand the mode of action of introgressed CRKN resistance in PA99N82-4 in comparison to the CRKN susceptible variety Russet Burbank. RESULTS:Histological studies revealed that the nematode juveniles successfully infect both resistant and susceptible root tissue by 48 h post inoculation, but the host resistance response restricts nematode feeding site formation in PA99N82-4. Differential gene expression analysis shows that 1268, 1261, 1102 and 2753 genes were up-regulated in PA99N82-4 at 48 h, 7 days, 14 days and 21 days post inoculation respectively, of which 61 genes were common across all the time points. These genes mapped to plant-pathogen interaction, plant hormonal signaling, antioxidant activity and cell wall re-enforcement pathways annotated for potato. CONCLUSION:The introgressed nematode resistance in PA99N82-4 is in the form of both pattern-triggered immune response and effector-triggered immune response, which is mediated by accumulation of reactive oxygen species and hypersensitive response (HR). Salicylic acid is playing a major role in the HR. Polyamines and suberin (a component of the Casperian strip in roots) also play an important role in mediating the resistance response. The present study provides the first ever comprehensive insights into transcriptional changes among M. chitwoodi resistant and susceptible potato genotypes after nematode inoculation. The knowledge generated in the present study has implications in breeding for CRKN resistance in potato.
journal_name
BMC Genomicsjournal_title
BMC genomicsauthors
Bali S,Vining K,Gleason C,Majtahedi H,Brown CR,Sathuvalli Vdoi
10.1186/s12864-019-6257-1subject
Has Abstractpub_date
2019-11-28 00:00:00pages
907issue
1issn
1471-2164pii
10.1186/s12864-019-6257-1journal_volume
20pub_type
杂志文章相关文献
BMC GENOMICS文献大全abstract:BACKGROUND:Mango fruits contain a broad spectrum of phenolic compounds which impart potential health benefits; their biosynthesis is catalysed by enzymes in the phenylpropanoid-flavonoid (PF) pathway. The aim of this study was to reveal the variability in genes involved in the PF pathway in three different mango variet...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1784-x
更新日期:2015-07-30 00:00:00
abstract:BACKGROUND:Mammalian genomes contain a large number (approximately 1000) of olfactory receptor (OR) genes, many of which (20 to 50%) are pseudogenes. OR gene transcription is not restricted to the olfactory epithelium, but is found in numerous tissues. Using microarray hybridization and RTqPCR, we analyzed the mRNA pro...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-10-572
更新日期:2009-12-02 00:00:00
abstract:BACKGROUND:Drought is a lifestyle disease. Plant metabolomics has been exercised for understanding the fine-tuning of the potential pathways to surmount the adverse effects of drought stress. A broad spectrum of morphological and metabolic responses from seven Triticeae species including wild types with different droug...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-4321-2
更新日期:2017-12-15 00:00:00
abstract:BACKGROUND:The Andean cultivar Paloma is resistant to Mesoamerican and Andean races of Colletotrichum lindemuthianum, the fungal pathogen that causes the destructive anthracnose disease in common bean. Remarkably, Paloma is resistant to Mesoamerican races 2047 and 3481, which are among the most virulent races of the an...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-3685-7
更新日期:2017-04-18 00:00:00
abstract:BACKGROUND:Complete or near-complete genomic sequence information is presently only available for a few plant species representing a large phylogenetic diversity among plants. In order to effectively transfer this information to species lacking sequence information, comparative genomic tools need to be developed. Molec...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-7-207
更新日期:2006-08-14 00:00:00
abstract:BACKGROUND:Bacterial spore germination is a developmental process during which all required metabolic pathways are restored to transfer cells from their dormant state into vegetative growth. Streptomyces are soil dwelling filamentous bacteria with complex life cycle, studied mostly for they ability to synthesize second...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-1173
更新日期:2014-12-23 00:00:00
abstract:BACKGROUND:Microarray technology is limited to monitoring the expression of previously annotated genes that have corresponding probes on the array. Computationally annotated genes have not fully been validated, because ESTs and full-length cDNAs cannot cover entire transcribed regions. Here, mRNA-Seq (an Illumina cDNA ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-683
更新日期:2010-12-02 00:00:00
abstract:BACKGROUND:Armigeres subalbatus is a natural vector of the filarial worm Brugia pahangi, but it rapidly and proficiently kills Brugia malayi microfilariae by melanotic encapsulation. Because B. malayi and B. pahangi are morphologically and biologically similar, the Armigeres-Brugia system serves as a valuable model for...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-8-463
更新日期:2007-12-18 00:00:00
abstract:BACKGROUND:BCG is the most widely used vaccine of all time and remains the only licensed vaccine for use against tuberculosis in humans. BCG also protects other species such as cattle against tuberculosis, but due to its incompatibility with current tuberculin testing regimens remains unlicensed. BCG's efficacy relates...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5791-1
更新日期:2019-05-28 00:00:00
abstract:BACKGROUND:The importance of Indian germplasm as origin and primary center of diversity of cultivated melon is widely accepted. Genetic diversity of several collections from Indian has been studied previously, although an integrated analysis of these collections in a global diversity perspective has not been possible. ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5784-0
更新日期:2019-06-03 00:00:00
abstract:BACKGROUND:Gene expression profiling is a promising approach to better estimate patient prognosis; however, there are still unresolved problems, including little overlap among similarly developed gene sets and poor performance of a developed gene set in other datasets. RESULTS:We applied a gene sets approach to develo...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-9-177
更新日期:2008-04-16 00:00:00
abstract:BACKGROUND:The genus Populus includes poplars, aspens and cottonwoods, which will be collectively referred to as poplars hereafter unless otherwise specified. Poplars are the dominant tree species in many forest ecosystems in the Northern Hemisphere and are of substantial economic value in plantation forestry. Poplar h...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-9-57
更新日期:2008-01-29 00:00:00
abstract:BACKGROUND:Ancestry informative markers (AIMs) are a type of genetic marker that is informative for tracing the ancestral ethnicity of individuals. Application of AIMs has gained substantial attention in population genetics, forensic sciences, and medical genetics. Single nucleotide polymorphisms (SNPs), the materials ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-346
更新日期:2012-07-28 00:00:00
abstract:BACKGROUND:Roche 454 pyrosequencing has become a method of choice for generating transcriptome data from non-model organisms. Once the tens to hundreds of thousands of short (250-450 base) reads have been produced, it is important to correctly assemble these to estimate the sequence of all the transcripts. Most transcr...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-571
更新日期:2010-10-16 00:00:00
abstract:BACKGROUND:Small RNA (sRNA) sequences are known to have a broad impact on gene regulation by various mechanisms. Their performance for the prediction of hybrid traits has not yet been analyzed. Our objective was to analyze the relation of parental sRNA expression with the performance of their hybrids, to develop a sRNA...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-4708-8
更新日期:2018-05-21 00:00:00
abstract:BACKGROUND:Europeans and American Indians were major genetic ancestry of Hispanics in the U.S. These ancestral groups have markedly different incidence rates and outcomes in many types of cancers. Therefore, the genetic admixture may cause biased genetic association study with cancer susceptibility variants specificall...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-6333-6
更新日期:2019-12-30 00:00:00
abstract:BACKGROUND:Bacteriophages that infect the opportunistic pathogen Pseudomonas aeruginosa have been classified into several groups. One of them, which includes temperate phage particles with icosahedral heads and long flexible tails, bears genomes whose architecture and replication mechanism, but not their nucleotide seq...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-1146
更新日期:2014-12-19 00:00:00
abstract:BACKGROUND:The Gram-negative bacterium Chlamydia pneumoniae (Cpn) is the leading intracellular human pathogen responsible for respiratory infections such as pneumonia and bronchitis. Basic and applied research in pathogen biology, especially the elaboration of new mechanism-based anti-pathogen strategies, target discov...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-13-632
更新日期:2012-11-16 00:00:00
abstract:BACKGROUND:Reads assignment to taxonomic units is a key step in microbiome analysis pipelines. To date, accurate taxonomy annotation of 16S reads, particularly at species rank, is still challenging due to the short size of read sequences and differently curated classification databases. The close phylogenetic relations...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-5914-8
更新日期:2019-07-08 00:00:00
abstract:BACKGROUND:Systems biology and functional genomics require genome-wide datasets and resources. Complete sets of cloned open reading frames (ORFs) have been made for about a dozen bacterial species and allow researchers to express and study complete proteomes in a high-throughput fashion. RESULTS:We have constructed an...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-11-470
更新日期:2010-08-11 00:00:00
abstract:BACKGROUND:Genomic studies such as genome-wide association and genomic selection require genome-wide genotype data. All existing technologies used to create these data result in missing genotypes, which are often then inferred using genotype imputation software. However, existing imputation methods most often make use ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-017-3873-5
更新日期:2017-07-10 00:00:00
abstract:BACKGROUND:Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeutic potential of genomic editing technologies. Targeted PCR amplification combined with Next Generation Sequencing (NGS) or enzymatic digestion, while broadly used in the genome editing field...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-018-4561-9
更新日期:2018-03-21 00:00:00
abstract:BACKGROUND:The obligate intracellular parasite Toxoplasma gondii establishes a life-long chronic infection within any warm-blooded host. After ingestion of an encysted parasite, T. gondii disseminates throughout the body as a rapidly replicating form during acute infection. Over time and after stimulation of the host i...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-806
更新日期:2014-09-20 00:00:00
abstract:BACKGROUND:Acinetobacter baumannii is a major health problem. The most common infection caused by A. baumannii is hospital acquired pneumonia, and the associated mortality rate is approximately 50%. Neither in vivo nor ex vivo expression profiling has been performed at the proteomic or transcriptomic level for pneumoni...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-015-1608-z
更新日期:2015-05-30 00:00:00
abstract:BACKGROUND:The unfolded protein response (UPR) is a network of intracellular signaling pathways that supports the ability of the secretory pathway to maintain a balance between the load of proteins entering the endoplasmic reticulum (ER) and the protein folding capacity of the ER lumen. Current evidence indicates that ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-159
更新日期:2014-02-25 00:00:00
abstract:BACKGROUND:Alpha tubulin is a fundamental component of the cytoskeleton which is responsible for cell shape and is involved in cell division, ciliary and flagellar motility and intracellular transport. Alpha tubulin gene expression varies according to the morphological changes suffered by Leishmania in its life cycle. ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-14-454
更新日期:2013-07-06 00:00:00
abstract:BACKGROUND:The laying hen model of spontaneous epithelial ovarian cancer (EOC) is unique in that it is the only model that enables observations of early events in disease progression and is therefore also uniquely suited for chemoprevention trials. Previous studies on the effect of dietary flaxseed in laying hens have ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-15-709
更新日期:2014-08-24 00:00:00
abstract:BACKGROUND:Citrus blight is a very important progressive decline disease of commercial citrus. The etiology is unknown, although the disease can be transmitted by root grafts, suggesting a viral etiology. Diagnosis is made by demonstrating physical blockage of xylem cells that prevents the movement of water. This test ...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-019-6339-0
更新日期:2019-12-11 00:00:00
abstract:BACKGROUND:Diversification on the basis of utilization is a hallmark of Beta vulgaris (beet), as well as other crop species. Often, crop improvement and management activities are segregated by crop type, thus preserving unique genome diversity and organization. Full interfertility is typically retained in crosses betwe...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/s12864-020-6451-1
更新日期:2020-03-02 00:00:00
abstract:BACKGROUND:Sorghum belongs to the tribe of the Andropogoneae that includes potential biofuel crops like switchgrass, Miscanthus and successful biofuel crops like corn and sugarcane. However, from a genomics point of view sorghum has compared to these other species a simpler genome because it lacks the additional rounds...
journal_title:BMC genomics
pub_type: 杂志文章
doi:10.1186/1471-2164-12-356
更新日期:2011-07-08 00:00:00