Abstract:
BACKGROUND:Celery is a widely cultivated vegetable abundant in ascorbate (AsA), a natural plant antioxidant capable of scavenging free radicals generated by abiotic stress in plants. Ascorbate peroxidase (APX) is a plant antioxidant enzyme that is important in the synthesis of AsA and scavenging of excess hydrogen peroxide. However, the characteristics and functions of APX in celery remain unclear to date. RESULTS:In this study, a gene encoding APX was cloned from celery and named AgAPX1. The transcription level of the AgAPX1 gene was significantly upregulated under drought stress. AgAPX1 was expressed in Escherichia coli BL21 (DE3) and purified. The predicted molecular mass of rAgAPX1 was 33.16 kDa, which was verified by SDS-PAGE assay. The optimum pH and temperature for rAgAPX1 were 7.0 and 55 °C, respectively. Transgenic Arabidopsis hosting the AgAPX1 gene showed elevated AsA content, antioxidant capacity and drought resistance. Less decrease in net photosynthetic rate, chlorophyll content, and relative water content contributed to the high survival rate of transgenic Arabidopsis lines after drought. CONCLUSIONS:The characteristics of APX in celery were different from that in other species. The enhanced drought resistance of overexpressing AgAPX1 in Arabidopsis may be achieved by increasing the accumulation of AsA, enhancing the activities of various antioxidant enzymes, and promoting stomatal closure. Our work provides new evidence to understand APX and its response mechanisms to drought stress in celery.
journal_name
BMC Plant Bioljournal_title
BMC plant biologyauthors
Liu JX,Feng K,Duan AQ,Li H,Yang QQ,Xu ZS,Xiong ASdoi
10.1186/s12870-019-2095-1subject
Has Abstractpub_date
2019-11-11 00:00:00pages
488issue
1issn
1471-2229pii
10.1186/s12870-019-2095-1journal_volume
19pub_type
杂志文章abstract:BACKGROUND:Wheat seeds provide a staple food and an important protein source for the world's population. Seed germination is vital to wheat growth and development and directly affects grain yield and quality. In this study, we performed the first comparative proteomic analysis of wheat embryo and endosperm during seed ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-015-0471-z
更新日期:2015-04-08 00:00:00
abstract:BACKGROUND:PLATZ proteins are a novel class of plant-specific zinc-dependent DNA-binding proteins that are classified as transcription factors (TFs). However, their common biochemical features and functions are poorly understood. RESULT:Here, we identified and cloned 17 PLATZ genes in the maize (Zea mays) genome. All ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1443-x
更新日期:2018-10-05 00:00:00
abstract:BACKGROUND:Proper development of plastids in embryo and seedling tissues is critical for plant development. During germination, plastids develop to perform many critical functions that are necessary to establish the seedling for further growth. A growing body of work has demonstrated that components of the plastid tran...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-7-37
更新日期:2007-07-13 00:00:00
abstract:BACKGROUND:In order to grow, plants rely on soil nutrients which can vary both spatially and temporally depending on the environment, the soil type or the microbial activity. An essential nutrient is nitrogen, which is mainly accessible as nitrate and ammonium. Many studies have investigated transport genes for these i...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1768-0
更新日期:2019-05-20 00:00:00
abstract:BACKGROUND:There are two plant phosphorus (P)-uptake pathways, namely the direct P uptake by roots and the indirect P uptake through arbuscular mycorrhizal fungi (AMF). Maximizing the efficiency of root and AMF processes associated with P acquisition by adjusting soil conditions is important for simultaneously ensuring...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1550-8
更新日期:2018-12-05 00:00:00
abstract:BACKGROUND:Drought stress in juvenile stages of crop development and premature leaf senescence induced by drought stress have an impact on biomass production and yield formation of barley (Hordeum vulgare L.). Therefore, in order to get information of regulatory processes involved in the adaptation to drought stress an...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-015-0701-4
更新日期:2016-01-05 00:00:00
abstract:BACKGROUND:Chrysanthemum, a leading ornamental species, does not tolerate salinity stress, although some of its related species do. The current level of understanding regarding the mechanisms underlying salinity tolerance in this botanical group is still limited. RESULTS:A comparison of the physiological responses to ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-016-0781-9
更新日期:2016-04-21 00:00:00
abstract:BACKGROUND:Modifications of DNA and histones in various combinations are correlated with many cellular processes. In this study, we investigated the possible relationship between histone H4 tetraacetylation, DNA methylation and histone H3 dimethylation at lysine 9 during mitosis in maize root meristems. RESULTS:Treatm...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-178
更新日期:2010-08-18 00:00:00
abstract:BACKGROUND:Invertases are ubiquitous enzymes that irreversibly cleave sucrose into fructose and glucose. Plant invertases play important roles in carbohydrate metabolism, plant development, and biotic and abiotic stress responses. In potato (Solanum tuberosum), invertases are involved in 'cold-induced sweetening' of tu...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-271
更新日期:2010-12-09 00:00:00
abstract:BACKGROUND:Isoprenylcysteine methylesterases (ICME) demethylate prenylated protein in eukaryotic cell. Until now, knowledge about their molecular information, localization and expression pattern is largely unavailable in plant species. One ICME in Arabidopsis, encoded by At5g15860, has been identified recently. Over-ex...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-212
更新日期:2010-09-27 00:00:00
abstract:BACKGROUND:Epigenetic marks superimposed on the DNA sequence of eukaryote chromosomes provide agility and plasticity in terms of modulating gene expression, ontology, and response to the environment. Modulating the methylation status of cytosine can generate epialleles, which have been detected and characterised at spe...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-12-193
更新日期:2012-10-20 00:00:00
abstract:BACKGROUND:Flowering at an appropriate time is crucial for seed maturity and reproductive success in all flowering plants. Soybean (Glycine max) is a typical short day plant, and both photoperiod and autonomous pathway genes exist in soybean genome. However, little is known about the functions of soybean autonomous pat...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-014-0263-x
更新日期:2014-10-07 00:00:00
abstract:BACKGROUND:Mulberry trees are the most important host for rearing mulberry silkworms in sericulture. Improved varieties of mulberry tree have been developed through traditional breeding procedures. Not much work, however, has been carried out on the molecular characterization of these varieties. Random Amplified Polymo...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-1-3
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Phased small interfering RNA (phasiRNA) is primarily derived from the 22-nt miRNA targeting loci. GhMYB2, a gene with potential roles in cotton fiber cell fate determination, is a target gene of miR828 and miR858 in the generation of phasiRNAs. RESULTS:In the presented work, through the evaluation of phasin...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-02430-3
更新日期:2020-05-15 00:00:00
abstract:BACKGROUND:The selective removal of grapevine leaves around berry clusters can improve the quality of ripening fruits by influencing parameters such as the berry sugar and anthocyanin content at harvest. The outcome depends strongly on the timing of defoliation, which influences the source-sink balance and the modified...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-30
更新日期:2013-02-22 00:00:00
abstract:BACKGROUND:Wheat grain avenin-like proteins (ALPs) belong to a recently discovered class of wheat grain storage protein. ALPs in wheat grains not only have beneficial effects on dough quality but also display antifungal activities, which is a novel observation for wheat storage proteins. Previous studies have shown tha...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-2259-z
更新日期:2020-01-29 00:00:00
abstract:BACKGROUND:Salt stress is one of the major abiotic stresses affecting plant growth and productivity. Vacuolar H+-pyrophosphatase (H+-PPase) genes play an important role in salt stress tolerance in multiple species. RESULTS:In this study, the promoter from the vacuolar H+-pyrophosphatase from Thellungiella halophila (T...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-90
更新日期:2010-05-18 00:00:00
abstract:BACKGROUND:The modification effect of leaching fraction (LF) on the physiological responses of plants to irrigation water salinity (ECiw) remains unknown. Here, leaf gas exchange, photosynthetic light-response and CO2-response curves, and total carbon (C) and nitrogen (N) accumulation in hot pepper leaves were investig...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1910-z
更新日期:2019-07-08 00:00:00
abstract:BACKGROUND:PsbS is a 22-kDa Photosystem (PS) II protein involved in non-photochemical quenching (NPQ) of chlorophyll fluorescence. Rice (Oryza sativa L.) has two PsbS genes, PsbS1 and PsbS2. However, only inactivation of PsbS1, through a knockout (PsbS1-KO) or in RNAi transgenic plants, results in plants deficient in q...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-014-0242-2
更新日期:2014-10-24 00:00:00
abstract:BACKGROUND:Long terminal repeat (LTR) retrotransposons are highly abundant in plant genomes and require transcriptional activity for their proliferative mode of replication. These sequences exist in plant genomes as diverse sublineages within the main element superfamilies (i.e., gypsy and copia). While transcriptional...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-017-1223-z
更新日期:2018-01-05 00:00:00
abstract:BACKGROUND:Plant lipid transfer proteins (LTPs) assemble a family of small (7-9 kDa) ubiquitous cationic proteins with an ability to bind and transport lipids as well as participate in various physiological processes including defense against phytopathogens. They also form one of the most clinically relevant classes of...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-016-0792-6
更新日期:2016-04-30 00:00:00
abstract:BACKGROUND:Mitogen-activated protein kinase kinase kinases (MAPKKKs; MAP3Ks) are important components of MAPK cascades, which are highly conserved signal transduction pathways in animals, yeast and plants, play important roles in plant growth and development. MAPKKKs have been investigated on their evolution and expres...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-014-0219-1
更新日期:2014-08-27 00:00:00
abstract:BACKGROUND:ABA-mediated processes are involved in plant responses to water deficit, especially the control of stomatal opening. However in grapevine it is not known if these processes participate in the phenotypic variation in drought adaptation existing between genotypes. To elucidate this question, the response to sh...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-016-0778-4
更新日期:2016-04-18 00:00:00
abstract:BACKGROUND:The R2R3-MYB genes comprise one of the largest transcription factor gene families in plants, playing regulatory roles in plant-specific developmental processes, metabolite accumulation and defense responses. Although genome-wide analysis of this gene family has been carried out in some species, the R2R3-MYB ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-014-0249-8
更新日期:2014-09-25 00:00:00
abstract:BACKGROUND:The plant nuclear pore complex has strongly attracted the attention of the scientific community during the past few years, in particular because of its involvement in hormonal and pathogen/symbiotic signalling. In Arabidopsis thaliana, more than 30 nucleoporins have been identified, but only a few of them ha...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-015-0695-y
更新日期:2016-01-05 00:00:00
abstract:BACKGROUND:Sugar beet is a highly salt-tolerant crop. However, its ability to withstand high salinity is reduced compared to sea beet, a wild ancestor of all beet crops. The aim of this study was to investigate transcriptional patterns associated with physiological, cytological and biochemical mechanisms involved in sa...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1661-x
更新日期:2019-02-06 00:00:00
abstract:BACKGROUND:Somatic embryogenesis (SE) is an asexual propagation pathway requiring a somatic-to-embryonic transition of differentiated somatic cells toward embryogenic cells capable of producing embryos in a process resembling zygotic embryogenesis. In chicory, genetic variability with respect to the formation of somati...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-7-27
更新日期:2007-06-06 00:00:00
abstract:BACKGROUND:Leaves are determinate organs; hence, precise control of cell proliferation and post-mitotic cell expansion is essential for their growth. A defect in cell proliferation often triggers enhanced post-mitotic cell expansion in leaves. This phenomenon is referred to as 'compensation'. Several lines of evidence ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-143
更新日期:2013-09-28 00:00:00
abstract:BACKGROUND:The acid component of grape berries, originating in the metabolism of malate and tartrate, the latter being less well-known than the former, is a key factor at play in the microbiological stability of wines destined for distillation. Grape acidity is increasingly affected by climate changes. The ability to c...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-016-0833-1
更新日期:2016-06-28 00:00:00
abstract:BACKGROUND:Epigenetic factors such as DNA methylation and histone modifications regulate a wide range of processes in plant development. Cytosine methylation and demethylation exist in a dynamic balance and have been associated with gene silencing or activation, respectively. In Arabidopsis, cytosine demethylation is a...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-172
更新日期:2013-10-31 00:00:00