当前位置： SCI文献检索 > BMC PLANT BIOLOGY期刊下所有文献 > A hypomethylated population of Brassica rapa for forward and reverse epi-genetics.

A hypomethylated population of Brassica rapa for forward and reverse epi-genetics.

Abstract：

BACKGROUND:Epigenetic marks superimposed on the DNA sequence of eukaryote chromosomes provide agility and plasticity in terms of modulating gene expression, ontology, and response to the environment. Modulating the methylation status of cytosine can generate epialleles, which have been detected and characterised at specific loci in several plant systems, and have the potential to generate novel and relatively stable phenotypes. There have been no systematic attempts to explore and utilise epiallelic variation, and so extend the range of phenotypes available for selection in crop improvement. We developed an approach for generating novel epialleles by perturbation of the DNA methylation status. 5- Azacytidine (5-AzaC) provides selective targeting of 5 mCG, which in plants is associated with exonic DNA. Targeted chemical intervention using 5-AzaC has advantages over transgenic or mutant modulation of methyltransferases, allowing stochastic generation of epialleles across the genome. RESULTS:We demonstrate the potential of stochastic chemically-induced hypomethylation to generate novel and valuable variation for crop improvement. Systematic analysis of dose-response to 5-AzaC in B. rapa guided generation of a selfed stochastically hypomethylated population, used for forward screening of several agronomic traits. Dose-response was sigmoidal for several traits, similar to that observed for chemical mutagens such as EMS. We demonstrated transgenerational inheritance of some phenotypes. BraRoAZ is a unique hypomethylated population of 1000 E2 sib lines. When compared to untreated controls, 5-Aza C-treated lines exhibited reduced immuno-staining of 5mC on pachytene chromosomes, and Methylation Sensitive Amplified Polymorphism (MSAP) profiles that were both divergent and more variable. There was coincident phenotypic variation among these lines for a range of seed yield and composition traits, including increased seed protein content and decreased oil content, as well as decreased erucic acid and corresponding increases in linoleic and/or palmitic acid. Each 5-AzaC-treated line represents a unique combination of hypomethylated epialleles. CONCLUSIONS:The approach and populations developed are available for forward and reverse screening of epiallelic variation and subsequent functional and inheritance studies. The generation of stochastically hypomethylated populations has utility in epiallele discovery for a wide range of crop plants, and has considerable potential as an intervention strategy for crop improvement.

journal_name

BMC Plant Biol

journal_title

BMC plant biology

authors

Amoah S,Kurup S,Rodriguez Lopez CM,Welham SJ,Powers SJ,Hopkins CJ,Wilkinson MJ,King GJ

doi

10.1186/1471-2229-12-193

subject

Has Abstract

pub_date

2012-10-20 00:00:00

pages

193

issn

1471-2229

pii

1471-2229-12-193

journal_volume

pub_type

杂志文章

在线工具

A hypomethylated population of Brassica rapa for forward and reverse epi-genetics.