Abstract:
BACKGROUND:Invertases are ubiquitous enzymes that irreversibly cleave sucrose into fructose and glucose. Plant invertases play important roles in carbohydrate metabolism, plant development, and biotic and abiotic stress responses. In potato (Solanum tuberosum), invertases are involved in 'cold-induced sweetening' of tubers, an adaptive response to cold stress, which negatively affects the quality of potato chips and French fries. Linkage and association studies have identified quantitative trait loci (QTL) for tuber sugar content and chip quality that colocalize with three independent potato invertase loci, which together encode five invertase genes. The role of natural allelic variation of these genes in controlling the variation of tuber sugar content in different genotypes is unknown. RESULTS:For functional studies on natural variants of five potato invertase genes we cloned and sequenced 193 full-length cDNAs from six heterozygous individuals (three tetraploid and three diploid). Eleven, thirteen, ten, twelve and nine different cDNA alleles were obtained for the genes Pain-1, InvGE, InvGF, InvCD141 and InvCD111, respectively. Allelic cDNA sequences differed from each other by 4 to 9%, and most were genotype specific. Additional variation was identified by single nucleotide polymorphism (SNP) analysis in an association-mapping population of 219 tetraploid individuals. Haplotype modeling revealed two to three major haplotypes besides a larger number of minor frequency haplotypes. cDNA alleles associated with chip quality, tuber starch content and starch yield were identified. CONCLUSIONS:Very high natural allelic variation was uncovered in a set of five potato invertase genes. This variability is a consequence of the cultivated potato's reproductive biology. Some of the structural variation found might underlie functional variation that influences important agronomic traits such as tuber sugar content. The associations found between specific invertase alleles and chip quality, tuber starch content and starch yield will facilitate the selection of superior potato genotypes in breeding programs.
journal_name
BMC Plant Bioljournal_title
BMC plant biologyauthors
Draffehn AM,Meller S,Li L,Gebhardt Cdoi
10.1186/1471-2229-10-271subject
Has Abstractpub_date
2010-12-09 00:00:00pages
271issn
1471-2229pii
1471-2229-10-271journal_volume
10pub_type
杂志文章abstract:BACKGROUND:Recurrent gene duplication and retention played an important role in angiosperm genome evolution. It has been hypothesized that these processes contribute significantly to plant adaptation but so far this hypothesis has not been tested at the genome scale. RESULTS:We studied available sequenced angiosperm g...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-14-151
更新日期:2014-05-31 00:00:00
abstract:BACKGROUND:Isoprenylcysteine methylesterases (ICME) demethylate prenylated protein in eukaryotic cell. Until now, knowledge about their molecular information, localization and expression pattern is largely unavailable in plant species. One ICME in Arabidopsis, encoded by At5g15860, has been identified recently. Over-ex...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-212
更新日期:2010-09-27 00:00:00
abstract:BACKGROUND:Homeodomain-leucine zipper (HD-ZIP) transcription factors play important roles in the growth, development and stress responses of plants, including (presumably) physic nut (Jatropha curcas), which has high drought and salinity tolerance. However, although physic nut's genome has been released, there is littl...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1920-x
更新日期:2019-07-08 00:00:00
abstract:BACKGROUND:The normal growth of Rehmannia glutinosa, a widely used medicinal plant in China, is severely disturbed by replant disease. The formation of replant disease commonly involves interactions among plants, allelochemicals and microbes; however, these relationships remain largely unclear. As a result, no effectiv...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-017-1060-0
更新日期:2017-07-10 00:00:00
abstract:BACKGROUND:Sesame (Sesame indicum L.) is well-known as a versatile industrial crop having various usages and contains 50-55% oil, 20% protein, 14-20% carbohydrate and 2-3% fiber. Several environmental factors are known to adversely affect yield and productivity of sesame. Our overall aim was to improve the growth, yiel...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-2077-3
更新日期:2019-11-04 00:00:00
abstract:BACKGROUND:Leaves are determinate organs; hence, precise control of cell proliferation and post-mitotic cell expansion is essential for their growth. A defect in cell proliferation often triggers enhanced post-mitotic cell expansion in leaves. This phenomenon is referred to as 'compensation'. Several lines of evidence ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-143
更新日期:2013-09-28 00:00:00
abstract:BACKGROUND:Calophyllum brasiliense is highlighted as an important resource of calanolides, which are dipyranocoumarins that inhibit the reverse transcriptase of human immunodeficiency virus type 1 (HIV-1 RT). Despite having great medicinal importance, enzymes involved in calanolide, biosynthesis and the pathway itself,...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-016-0862-9
更新日期:2016-08-15 00:00:00
abstract:BACKGROUND:Cotton fibre quality traits such as fibre length, strength, and degree of maturation are determined by genotype and environment during the sequential phases of cotton fibre development (cell elongation, transition to secondary cell wall construction and cellulose deposition). The cotton fibre middle lamella ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-017-1017-3
更新日期:2017-03-31 00:00:00
abstract:BACKGROUND:Water permeability governed by seed coat is a major facet of seed crops, especially soybean, whose seeds lack physiological dormancy and experience rapid deterioration in seed viability under prolonged storage. Moreover, the physiological and chemical characteristics of soybean seeds are known to vary with s...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1341-2
更新日期:2018-07-09 00:00:00
abstract:BACKGROUND:Plant lipid transfer proteins (LTPs) assemble a family of small (7-9 kDa) ubiquitous cationic proteins with an ability to bind and transport lipids as well as participate in various physiological processes including defense against phytopathogens. They also form one of the most clinically relevant classes of...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-016-0792-6
更新日期:2016-04-30 00:00:00
abstract:BACKGROUND:While floral symmetry has traditionally been assessed qualitatively, recent advances in geometric morphometrics have opened up new avenues to specifically quantify flower shape and size using robust multivariate statistical methods. In this study, we examine, for the first time, the ability of geometric morp...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-017-1152-x
更新日期:2017-11-17 00:00:00
abstract:BACKGROUND:It is known that hexaploid common wheat (Triticum aestivum L.) has stronger adaptability to many stressful environments than its tetraploid wheat progenitor. However, the physiological basis and evolutionary course to acquire these enhanced adaptabilities by common wheat remain understudied. Here, we aimed t...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1334-1
更新日期:2018-06-07 00:00:00
abstract:BACKGROUND:The arbuscular mycorrhizal symbiosis is characterized by the presence of different symbiotic structures and stages within a root system. Therefore tools allowing the analysis of molecular changes at a cellular level are required to reveal insight into arbuscular mycorrhizal (AM) symbiosis development and fun...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-015-0601-7
更新日期:2015-09-30 00:00:00
abstract:BACKGROUND:Mal d 1 is a major apple allergen causing food allergic symptoms of the oral allergy syndrome (OAS) in birch-pollen sensitised patients. The Mal d 1 gene family is known to have at least 7 intron-containing and 11 intronless members that have been mapped in clusters on three linkage groups. In this study, th...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-8-116
更新日期:2008-11-13 00:00:00
abstract:BACKGROUND:Real-time RT-PCR has become a powerful technique to monitor low-abundance mRNA expression and is a useful tool when examining bacterial gene expression inside infected host tissues. However, correct evaluation of data requires accurate and reliable normalisation against internal standards. Thus, the identifi...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-7-50
更新日期:2007-09-21 00:00:00
abstract:BACKGROUND:A positive relationship between genome size and intron length is observed across eukaryotes including Angiosperms plants, indicating a co-evolution of genome size and gene structure. Conifers have very large genomes and longer introns on average than most plants, but impacts of their large genome and longer ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-14-95
更新日期:2014-04-16 00:00:00
abstract:BACKGROUND:Glutathione S-transferases (GSTs) represent a ubiquitous gene family encoding detoxification enzymes able to recognize reactive electrophilic xenobiotic molecules as well as compounds of endogenous origin. Anthocyanin pigments require GSTs for their transport into the vacuole since their cytoplasmic retentio...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-14-39
更新日期:2014-02-03 00:00:00
abstract:BACKGROUND:UDP-glucose pyrophosphorylase (UGPase) is a sugar-metabolizing enzyme (E.C. 2.7.7.9) that catalyzes a reversible reaction of UDP-glucose and pyrophosphate from glucose-1-phosphate and UTP. UDP-glucose is a key intermediate sugar that is channeled to multiple metabolic pathways. The functional role of UGPase ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-014-0265-8
更新日期:2014-10-07 00:00:00
abstract:BACKGROUND:Manganese (Mn), an essential element for plants, can be toxic when present in excess. Stylo (Stylosanthes) is a pioneer tropical legume with great potential for Mn tolerance, but its Mn tolerance mechanisms remain poorly understood. RESULTS:In this study, variations in Mn tolerance were observed among nine ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1822-y
更新日期:2019-05-22 00:00:00
abstract:BACKGROUND:The thylakoid system in plant chloroplasts is organized into two distinct domains: grana arranged in stacks of appressed membranes and non-appressed membranes consisting of stroma thylakoids and margins of granal stacks. It is argued that the reason for the development of appressed membranes in plants is tha...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-12-72
更新日期:2012-05-25 00:00:00
abstract:BACKGROUND:Flowering at an appropriate time is crucial for seed maturity and reproductive success in all flowering plants. Soybean (Glycine max) is a typical short day plant, and both photoperiod and autonomous pathway genes exist in soybean genome. However, little is known about the functions of soybean autonomous pat...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-014-0263-x
更新日期:2014-10-07 00:00:00
abstract:BACKGROUND:Among various pests, the brown planthopper (BPH) that damages rice is the major destructive pests. Understanding resistance mechanisms is a critical step toward effective control of BPH. This study investigates the proteomics of BPH interactions with three rice cultivars: the first resistant (PR) to BPH, the...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1622-9
更新日期:2019-01-18 00:00:00
abstract:BACKGROUND:Phosphoenolpyruvate carboxylase (PEPC) is a critical enzyme catalyzing the β-carboxylation of phosphoenolpyruvate (PEP) to oxaloacetate, a tricarboxylic acid (TCA) cycle intermediate. PEPC typically exists as a Class-1 PEPC homotetramer composed of plant-type PEPC (PTPC) polypeptides, and two of the subunits...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-200
更新日期:2010-09-14 00:00:00
abstract:BACKGROUND:Low-molecular-weight glutenin subunits (LMW-GS), encoded by Glu-3 complex loci in hexaploid wheat, play important roles in the processing quality of wheat flour. To date, the molecular characteristics and effects on dough quality of individual Glu-3 alleles and their encoding proteins have been poorly studie...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-014-0367-3
更新日期:2014-12-19 00:00:00
abstract:BACKGROUND:Angiosperm sex chromosomes, where present, are generally recently evolved. The key step in initiating the development of sex chromosomes from autosomes is the establishment of a sex-determining locus within a region of non-recombination. To better understand early sex chromosome evolution, it is important to...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1766-2
更新日期:2019-04-30 00:00:00
abstract:BACKGROUND:Wheat stripe rust (caused by Puccinia striiformis f. sp. Tritici), is a major disease that causes huge yield damage. New pathogen races appeared in the last few years and caused a broke down in the resistant genotypes. In Egypt, some of the resistant genotypes began to be susceptible to stripe rust in recent...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-02813-6
更新日期:2021-01-14 00:00:00
abstract:BACKGROUND:Gynodioecious species exist in two sexes - male-sterile females and hermaphrodites. Male sterility in higher plants often results from mitonuclear interaction between the CMS (cytoplasmic male sterility) gene(s) encoded by mitochondrial genome and by nuclear-encoded restorer genes. Mitochondrial and nuclear-...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-2193-0
更新日期:2019-12-19 00:00:00
abstract:BACKGROUND:The green peach aphid (GPA), Myzus persicae, is economically one of the most threatening pests in pepper cultivation, which not only causes direct damage but also transmits many viruses. Breeding aphid resistant pepper varieties is a promising and environmentally friendly method to control aphid populations ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1340-3
更新日期:2018-06-27 00:00:00
abstract:BACKGROUND:Xylogen, a chimeric arabinogalactan protein containing a non-specific lipid transfer protein domain, can promote xylem cell differentiation. No comprehensive study has been carried out on the XYLP gene family in rice. As a first step in research on this gene family and as a useful strategy in general, a geno...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-014-0299-y
更新日期:2014-11-18 00:00:00
abstract:BACKGROUND:The effective functional analysis of male gametophyte development requires new tools enabling the spatially and temporally controlled expression of both marker genes and modified genes of interest. In particular, promoters driving expression at earlier developmental stages including microspores are required....
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-6-31
更新日期:2006-12-21 00:00:00