Symbiosis dependent accumulation of primary metabolites in arbuscule-containing cells.

Abstract:

BACKGROUND:The arbuscular mycorrhizal symbiosis is characterized by the presence of different symbiotic structures and stages within a root system. Therefore tools allowing the analysis of molecular changes at a cellular level are required to reveal insight into arbuscular mycorrhizal (AM) symbiosis development and functioning. RESULTS:Here we describe the analysis of metabolite pools in arbuscule-containing cells, which are the site of nutrient transfer between AM fungus and host plant. Laser capture microdissection (LCM) combined with gas chromatography mass spectrometry (GC-EI/TOF-MS) enabled the analysis of primary metabolite levels,which might be of plant or fungal origin, within these cells. CONCLUSIONS:High levels of the amino acids, aspartate, asparagine, glutamate, and glutamine, were observed in arbuscule-containing cells. Elevated amounts of sucrose and the steady-state of hexose levels indicated a direct assimilation of monosaccharides by the fungal partner.

journal_name

BMC Plant Biol

journal_title

BMC plant biology

authors

Gaude N,Bortfeld S,Erban A,Kopka J,Krajinski F

doi

10.1186/s12870-015-0601-7

subject

Has Abstract

pub_date

2015-09-30 00:00:00

pages

234

issn

1471-2229

pii

10.1186/s12870-015-0601-7

journal_volume

15

pub_type

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