Abstract:
BACKGROUND:Verticillium spp. are major pathogens of dicotyledonous plants such as cotton, tomato, olive or oilseed rape. Verticillium symptoms are often ambiguous and influenced by development and environment. The aim of the present study was to define disease and resistance traits of the complex Verticillium longisporum syndrome in Arabidopsis thaliana (L.) Heynh. A genetic approach was used to determine genetic, developmental and environmental factors controlling specific disease and resistance traits and to study their interrelations. RESULTS:A segregating F2/F3 population originating from ecotypes 'Burren' (Bur) and 'Landsberg erecta' (Ler) was established. Plants were root-dip inoculated and tested under greenhouse conditions. The Verticillium syndrome was dissected into components like systemic spread, stunting, development time and axillary branching. Systemic spread of V. longisporum via colonisation of the shoot was extensive in Ler; Bur showed a high degree of resistance against systemic spread. Fungal colonisation of the shoot apex was determined by (a) determining the percentage of plants from which the fungus could be re-isolated and (b) measuring fungal DNA content with quantitative real-time PCR (qPCR). Four quantitative trait loci (QTL) controlling systemic spread were identified for the percentage of plants showing fungal outgrowth, two of these QTL were confirmed with qPCR data. The degree of colonisation by V. longisporum was negatively correlated with development time. QTL controlling development time showed some overlap with QTL for resistance to systemic spread. Stunting depended on host genotype, development time and seasonal effects. Five QTL controlling this trait were identified which did not co-localize with QTL controlling systemic spread. V. longisporum induced increased axillary branching in Bur; two QTL controlling this reaction were found. CONCLUSIONS:Systemic spread of V. longisporum in the host as well as resistance to this major disease trait are described for the first time in natural A. thaliana accessions. This creates the possibility to study a major resistance mechanism against vascular pathogens in this model plant and to clone relevant genes of the involved pathways. Stunting resistance and resistance to systemic spread were controlled by different QTL and should be treated as separate traits. Developmental and environmental effects on pathogenesis and resistance need to be considered when designing and interpreting experiments in research and breeding.
journal_name
BMC Plant Bioljournal_title
BMC plant biologyauthors
Häffner E,Karlovsky P,Diederichsen Edoi
10.1186/1471-2229-10-235subject
Has Abstractpub_date
2010-11-02 00:00:00pages
235issn
1471-2229pii
1471-2229-10-235journal_volume
10pub_type
杂志文章abstract:BACKGROUND:Rapeseed (Brassica napus L.) and soybean (Glycine max L.) seeds are rich in both protein and oil, which are major sources of biofuels and nutrition. Although the difference in seed oil content between soybean (~ 20%) and rapeseed (~ 40%) exists, little is known about its underlying molecular mechanism. RESU...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1542-8
更新日期:2018-12-04 00:00:00
abstract:BACKGROUND:DnaJ proteins participate in many metabolic pathways through dynamic interactions with various components of these processes. The role of three small chloroplast-targeted DnaJ proteins, AtJ8 (At1 g80920), AtJ11 (At4 g36040) and AtJ20 (At4 g13830), was investigated here using knock-out mutants of Arabidopsis ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-43
更新日期:2010-03-07 00:00:00
abstract:BACKGROUND:Plant breeding has been proposed as one of the most effective and environmentally safe methods to control fungal infection and to reduce fumonisin accumulation. However, conventional breeding can be hampered by the complex genetic architecture of resistance to fumonisin accumulation and marker-assisted selec...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1759-1
更新日期:2019-04-27 00:00:00
abstract:BACKGROUND:Within the Arabidopsis genome, there are 272 cytochrome P450 monooxygenase (P450) genes. However, the biological functions of the majority of these P450s remain unknown. The CYP709B family of P450s includes three gene members, CYP709B1, CYP709B2 and CYP709B3, which have high amino acid sequence similarity an...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-169
更新日期:2013-10-28 00:00:00
abstract:BACKGROUND:Recent genome sequencing enables mega-base scale comparisons between related genomes. Comparisons between animals, plants, fungi, and bacteria demonstrate extensive synteny tempered by rearrangements. Within the legume plant family, glimpses of synteny have also been observed. Characterizing syntenic relatio...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-5-15
更新日期:2005-08-15 00:00:00
abstract:BACKGROUND:To gain insight into the regulation of fruit ascorbic acid (AsA) pool in tomatoes, a combination of metabolite analyses, non-labelled and radiolabelled substrate feeding experiments, enzyme activity measurements and gene expression studies were carried out in fruits of the 'low-' and 'high-AsA' tomato cultiv...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-12-239
更新日期:2012-12-17 00:00:00
abstract:BACKGROUND:Many flowering plants produce bicellular pollen. The two cells of the pollen grain are destined for separate fates in the male gametophyte, which provides a unique opportunity to study genetic interactions that govern guided single-cell polar expansion of the growing pollen tube and the coordinated control o...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-12-24
更新日期:2012-02-16 00:00:00
abstract:BACKGROUND:Stevia rebaudiana (Asteraceae), native from Paraguay, accumulates steviol glycosides (SGs) into its leaves. These compounds exhibit acaloric intense sweet taste which answers to consumer demands for reducing daily sugar intake. Despite the developpement of S. rebaudiana cultivation all over the world, the de...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-2061-y
更新日期:2019-10-21 00:00:00
abstract:BACKGROUND:Cultivated rice species (Oryza sativa L. and O. glaberrima Steud.) are generally considered among the crop species most sensitive to salt stress. A handful of lines are known to be tolerant, and a small number of these have been used extensively as donors in breeding programs. However, these donors use many ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-32
更新日期:2013-02-27 00:00:00
abstract:BACKGROUND:Pumilio RNA-binding proteins are evolutionarily conserved throughout eukaryotes and are involved in RNA decay, transport, and translation repression in the cytoplasm. Although a majority of Pumilio proteins function in the cytoplasm, two nucleolar forms have been reported to have a function in rRNA processin...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-02444-x
更新日期:2020-05-24 00:00:00
abstract:BACKGROUND:Heading time is one of the most important agronomic traits in wheat, as it largely affects both adaptation to different agro-ecological conditions and yield potential. Identification of genes underlying the regulation of wheat heading and the development of diagnostic markers could facilitate our understandi...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-020-02539-5
更新日期:2020-07-13 00:00:00
abstract:BACKGROUND:Zinc (Zn) deficiency is one of the most widespread mineral nutritional problems that affect normal development in plants. Because Zn cannot passively diffuse across cell membranes, it must be transported into intracellular compartments for all biological processes where Zn is required. Several members of the...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-12-111
更新日期:2012-07-23 00:00:00
abstract:BACKGROUND:Suaeda salsa L. (S. salsa) is an annual euhalophyte with high salt tolerance and high value as an oil crop, traditional Chinese medicine and vegetable. However, there are few comprehensive studies on the metabolomics of S. salsa under saline conditions. RESULTS:Seedlings of S. salsa were cultured with 0, 20...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-2006-5
更新日期:2019-09-06 00:00:00
abstract:BACKGROUND:ANGUSTIFOLIA (AN), one of the CtBP family proteins, plays a major role in microtubule-dependent cell morphogenesis. Microarray analysis of mammalian AN homologs suggests that AN might function as a transcriptional activator and regulator of a wide range of genes. Genetic characterization of AN mutants sugges...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-79
更新日期:2013-05-14 00:00:00
abstract:BACKGROUND:Salix caprea is a cold-tolerant pioneer species that is ecologically important in Europe and western and central Asia. However, little data is available on its population genetic structure and molecular ecology. We describe the levels of geographic population genetic structure in natural Irish populations of...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-014-0202-x
更新日期:2014-08-07 00:00:00
abstract:BACKGROUND:Plant matrix metalloproteinases (MMP) are conserved proteolytic enzymes found in a wide range of monocotyledonous and dicotyledonous plant species. Acting on the plant extracellular matrix, they play crucial roles in many aspects of plant physiology including growth, development and the response to stresses ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-9-83
更新日期:2009-06-29 00:00:00
abstract:BACKGROUND:The green peach aphid (GPA), Myzus persicae, is economically one of the most threatening pests in pepper cultivation, which not only causes direct damage but also transmits many viruses. Breeding aphid resistant pepper varieties is a promising and environmentally friendly method to control aphid populations ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-018-1340-3
更新日期:2018-06-27 00:00:00
abstract:BACKGROUND:Agrobacterium infection, which is widely used to generate transgenic plants, is often accompanied by T-DNA-linked mutations and transpositions in flowering plants. It is not known if Agrobacterium infection also affects the rates of point mutations, somatic homologous recombinations (SHR) and frame-shift mut...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-015-0595-1
更新日期:2015-08-26 00:00:00
abstract:BACKGROUND:Glutamine synthetase (GS) acts as a key enzyme in plant nitrogen (N) metabolism. It is important to understand the regulation of GS expression in plant. Promoters can initiate the transcription of its downstream gene. Eichhornia crassipes is a most prominent aquatic invasive plant, which has negative effects...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-021-02832-x
更新日期:2021-01-29 00:00:00
abstract:BACKGROUND:The identification of QTL involved in heterosis formation is one approach to unravel the not yet fully understood genetic basis of heterosis - the improved agronomic performance of hybrid F1 plants compared to their inbred parents. The identification of candidate genes underlying a QTL is important both for ...
journal_title:BMC plant biology
pub_type: 杂志文章,meta分析
doi:10.1186/1471-2229-14-88
更新日期:2014-04-02 00:00:00
abstract:BACKGROUND:APETALA2-like genes encode plant-specific transcription factors, some of which possess one microRNA172 (miR172) binding site. The miR172 and its target euAP2 genes are involved in the process of phase transformation and flower organ development in many plants. However, the roles of miR172 and its target AP2 ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-1936-2
更新日期:2019-08-01 00:00:00
abstract:BACKGROUND:The selective removal of grapevine leaves around berry clusters can improve the quality of ripening fruits by influencing parameters such as the berry sugar and anthocyanin content at harvest. The outcome depends strongly on the timing of defoliation, which influences the source-sink balance and the modified...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-13-30
更新日期:2013-02-22 00:00:00
abstract:BACKGROUND:Herbivory imposes an important selective pressure on plants. In Arabidopsis thaliana leaf trichomes provide a key defense against insect herbivory; however, trichome production incurs a fitness cost in the absence of herbivory. Previous work on A. thaliana has shown an increase in trichome density in respons...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-14-119
更新日期:2014-05-05 00:00:00
abstract:BACKGROUND:The thylakoid system in plant chloroplasts is organized into two distinct domains: grana arranged in stacks of appressed membranes and non-appressed membranes consisting of stroma thylakoids and margins of granal stacks. It is argued that the reason for the development of appressed membranes in plants is tha...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-12-72
更新日期:2012-05-25 00:00:00
abstract:BACKGROUND:Genome-wide association studies (GWAS) based on linkage disequilibrium (LD) provide a promising tool for the detection and fine mapping of quantitative trait loci (QTL) underlying complex agronomic traits. In this study we explored the genetic basis of variation for the traits heading date, plant height, tho...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-12-16
更新日期:2012-01-27 00:00:00
abstract:BACKGROUND:Plant-endophyte symbioses often revolve around nitrogen metabolism, and involve varying degrees of intimacy. Although evidence for vertical inheritance of nitrogen-fixing endophytic bacteria is increasing, it is confined mostly to crop plants, and to date no such system has been reported for geophytes. METH...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-019-2049-7
更新日期:2019-10-23 00:00:00
abstract:BACKGROUND:A positive relationship between genome size and intron length is observed across eukaryotes including Angiosperms plants, indicating a co-evolution of genome size and gene structure. Conifers have very large genomes and longer introns on average than most plants, but impacts of their large genome and longer ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-14-95
更新日期:2014-04-16 00:00:00
abstract:BACKGROUND:In cereal crops such as wheat, an optimal timing of developmental transitions is required to maximize grain yield. Many of these developmental changes are precisely regulated by changes in the duration, intensity or quality of light. Phytochromes are dimeric photoreceptors that absorb light maximally in the ...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-016-0831-3
更新日期:2016-06-21 00:00:00
abstract:BACKGROUND:Berry size is considered as one of the main selection criteria in table grape breeding programs. However, this is a quantitative and polygenic trait, and its genetic determination is still poorly understood. Considering its economic importance, it is relevant to determine its genetic architecture and elucida...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/s12870-016-0789-1
更新日期:2016-04-26 00:00:00
abstract:BACKGROUND:Genetic markers and linkage mapping are basic prerequisites for marker-assisted selection and map-based cloning. In the case of the key grassland species Lolium spp., numerous mapping populations have been developed and characterised for various traits. Although some genetic linkage maps of these populations...
journal_title:BMC plant biology
pub_type: 杂志文章
doi:10.1186/1471-2229-10-177
更新日期:2010-08-16 00:00:00