Halobacteriovorax vibrionivorans sp. nov., a novel prokaryotic predator isolated from coastal seawater of China.

Abstract:

:Three prokaryotic predator strains, BL9T, BL10 and BL28, were isolated with Vibrio alginolyticus from coastal seawater of PR China. Cells of the strains were Gram-negative, vibrioid-shaped and motile with a single sheathed flagellum (25-28 nm wide). Cells were around 0.3×0.5-1.0 µm in size. The three strains were obligate predators that exhibited a biphasic life cycle: a free-swimming attack phase and an intraperiplasmic growth phase within the prey. Bdelloplasts were formed. NaCl was required for growth. Optimum growth occurred at ~37 °C, with 2-4 % (w/v) NaCl and at pH 7.0-8.0. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that the three strains shared 99.9 % similarity to each other, were affiliated with the genus Halobacteriovorax in the class Oligoflexia, and represented the same new species. Strain BL9T (=MCCC 1K03527T=JCM 32962T) was designated as the type strain. Genome sequencing of strain BL9T revealed a genome size of 3.14 Mb and a G+C content of 35.8 mol%. The estimated digital DNA-DNA hybridization (dDDH) values and the whole genome average nucleotide identity (gANI) values between the genome of strain BL9T and those of Bdellovibrionales and Bacteriovoracales were 12.5-19 and 63.49-76.15 %, respectively. On the basis of life cycle features, results of physiological analyses, gANI data and dDDH data, strain BL9T represents a new species within the genus Halobacteriovorax, for which the name Halobacteriovoraxvibrionivorans sp. nov. is proposed.

authors

Ye XS,Chen MX,Li HY,He XY,Zhao Y

doi

10.1099/ijsem.0.003703

subject

Has Abstract

pub_date

2019-12-01 00:00:00

pages

3917-3923

issue

12

eissn

1466-5026

issn

1466-5034

journal_volume

69

pub_type

杂志文章
  • Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China.

    abstract::Two Gram-positive, rod-shaped, spore-forming and moderately halophilic bacteria (strains 28-1T, 28-4), isolated from a soil sample from a neutral salt lake in Xin-Jiang, China, were characterized polyphasically. On the basis of fasta (ungapped) analyses of 16S rRNA gene sequences, strains 28-1T and 28-4 were shown to ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63180-0

    authors: Ren PG,Zhou PJ

    更新日期:2005-01-01 00:00:00

  • Haloferax sulfurifontis sp. nov., a halophilic archaeon isolated from a sulfide- and sulfur-rich spring.

    abstract::A pleomorphic, extremely halophilic archaeon (strain M6(T)) was isolated from a sulfide- and sulfur-rich spring in south-western Oklahoma (USA). It formed small (0.8-1.0 mm), salmon pink, elevated colonies on agar medium. The strain grew in a wide range of NaCl concentrations (6 % to saturation) and required at least ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63211-0

    authors: Elshahed MS,Savage KN,Oren A,Gutierrez MC,Ventosa A,Krumholz LR

    更新日期:2004-11-01 00:00:00

  • Halostella pelagica sp. nov. and Halostella litorea sp. nov., isolated from salted brown alga Laminaria.

    abstract::Three rod-shaped halophilic archaeal strains, DL-M4T, LYG-109 and DLLS-108T, were isolated from the salted brown alga Laminaria produced in different marine areas of PR China. Cells of strains were motile, formed red-pigmented colonies on agar and lysed in distilled water. The three strains grew optimally with 2.6 M N...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004003

    authors: Han D,Cui HL

    更新日期:2020-03-01 00:00:00

  • Mycoplasma enhydrae sp. nov. isolated from southern sea otters (Enhydra lutris nereis).

    abstract::Five Mycoplasma strains have been isolated from the oropharynx of southern sea otters (Enhydra lutris nereis) from the Central California Coast, USA. These strains were phenotypically and genetically characterized and compared to other established Mycoplasma species. All five strains hydrolysed arginine but not urea, ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003144

    authors: Volokhov DV,Batac F,Gao Y,Miller M,Chizhikov VE

    更新日期:2019-02-01 00:00:00

  • Leucothrix arctica sp. nov., isolated from Arctic seawater.

    abstract::A Gram-stain-negative, non-motile, oxidase- and catalase-positive, rod-shaped bacterium was isolated from a coastal seawater sample from the Arctic Circle and designated strain IMCC9719T. On the basis of 16S rRNA gene sequence analysis, it was shown that strain IMCC9719T belonged to the genus Leucothrix and was closel...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003072

    authors: Baek K,Choi A,Lee YM,Lee HK,Cho JC

    更新日期:2018-12-01 00:00:00

  • Vibrio pacinii sp. nov., from cultured aquatic organisms.

    abstract::Three strains were isolated from cultured aquatic organisms. They were Gram-negative, oxidase-positive, motile, fermentative, arginine dihydrolase-positive, lysine and ornithine decarboxylase-negative and sensitive to vibriostatic agent O/129. These strains differ from other related Vibrio species by several phenotypi...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.02670-0

    authors: Gomez-Gil B,Thompson FL,Thompson CC,Swings J

    更新日期:2003-09-01 00:00:00

  • Rhodothermus profundi sp. nov., a thermophilic bacterium isolated from a deep-sea hydrothermal vent in the Pacific Ocean.

    abstract::Nine thermophilic strains of aerobic, non-sporulating, heterotrophic bacteria were isolated after enrichment of chimney material sampled from a deep-sea hydrothermal field at a depth of 2634m on the East-Pacific Rise (1 °N). The bacteria stained Gram-negative. They were rod-shaped and measured approximately 0.5μm in w...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.012724-0

    authors: Marteinsson VT,Bjornsdottir SH,Bienvenu N,Kristjansson JK,Birrien JL

    更新日期:2010-12-01 00:00:00

  • Allochromatium phaeobacterium sp. nov.

    abstract::A rod-shaped, phototrophic, purple sulfur bacterium was isolated in pure culture from brackish water near Bheemli, Visakhapatnam, India, in a medium that contained 2 % NaCl (w/v). Strain JA144(T) was Gram-negative and motile. It did not require salt, but tolerated up to 3 % NaCl (w/v). Intracellular photosynthetic mem...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65647-0

    authors: Srinivas TN,Anil Kumar P,Sucharitha K,Sasikala Ch,Ramana ChV

    更新日期:2009-04-01 00:00:00

  • Marortus luteolus gen. nov., sp. nov., isolated from surface seawater of the East Sea in China.

    abstract::A Gram-stain-negative, motile, aerobic, rod-shaped bacterium with flagella, designated ZX-21T, was isolated from surface seawater of the East Sea in Zhoushan, China. Growth of strain ZX-21T was observed at 10--35  o°C (optimum, 30 °C), at pH 6.0-8.5 (pHoptimum 6.5-7.0) and in the presence of 0.5-8 % (w/v) NaCl (optimu...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003348

    authors: Yu XD,Yu XY,Fu GY,Zhao Z,Shen X,Sun C,Wu M

    更新日期:2019-05-01 00:00:00

  • Kribbella catacumbae sp. nov. and Kribbella sancticallisti sp. nov., isolated from whitish-grey patinas in the catacombs of St Callistus in Rome, Italy.

    abstract::Several nocardioform actinomycetes were isolated from tufaceous surfaces with whitish-grey patinas in the catacombs of St Callistus in Rome, Italy. The morphology of the isolates and their chemotaxonomic characteristics such as LL-diaminopimelic acid in the cell-wall peptidoglycan, the major menaquinone of MK-9(H(4)),...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65613-0

    authors: Urzì C,De Leo F,Schumann P

    更新日期:2008-09-01 00:00:00

  • Erythrobacter citreus sp. nov., a yellow-pigmented bacterium that lacks bacteriochlorophyll a, isolated from the western Mediterranean Sea.

    abstract::Two facultatively oligotrophic, intensely yellow-pigmented bacterial strains, RE35F/1T and RE10F/45, have been previously isolated from the western Mediterranean Sea (Bay of Calvi, Corsica, France) by 0.2 microm membrane filtration. The organisms were gram-negative, catalase- and oxidase-positive, strictly aerobic, ro...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-52-5-1655

    authors: Denner EBM,Vybiral D,Koblízek M,Kämpfer P,Busse HJ,Velimirov B

    更新日期:2002-09-01 00:00:00

  • Niabella aurantiaca gen. nov., sp. nov., isolated from a greenhouse soil in Korea.

    abstract::An orange-coloured bacterial strain, designated R2A15-11(T), was isolated from greenhouse soil. The strain was found to be strictly aerobic, Gram-negative, non-spore-forming and non-flagellated. The cells were short rods (0.7-0.9x1.0-1.5 mum) and produced flexirubin. Growth of the strain was observed at 10-35 degrees ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64614-0

    authors: Kim BY,Weon HY,Yoo SH,Hong SB,Kwon SW,Stackebrandt E,Go SJ

    更新日期:2007-03-01 00:00:00

  • Chryseobacterium camelliae sp. nov., isolated from green tea.

    abstract::A Gram-staining-negative, strictly aerobic, non-motile, rod-shaped and flexirubin-type-pigmented strain, THG C4-1(T), was isolated from green tea leaves in Jangheung-gun, Republic of Korea. Strain THG C4-1(T) grew well at 20-30 °C, at pH 7.0-7.5 and in the absence of NaCl on nutrient agar. Based on 16S rRNA gene seque...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.057398-0

    authors: Kook M,Son HM,Ngo HTT,Yi TH

    更新日期:2014-03-01 00:00:00

  • Brucella papionis sp. nov., isolated from baboons (Papio spp.).

    abstract::Two Gram-negative, non-motile, non-spore-forming coccoid bacteria (strains F8/08-60(T) and F8/08-61) isolated from clinical specimens obtained from baboons (Papio spp.) that had delivered stillborn offspring were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence similarities, both strai...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.065482-0

    authors: Whatmore AM,Davison N,Cloeckaert A,Al Dahouk S,Zygmunt MS,Brew SD,Perrett LL,Koylass MS,Vergnaud G,Quance C,Scholz HC,Dick EJ,Hubbard G,Schlabritz-Loutsevitch NE

    更新日期:2014-12-01 00:00:00

  • Methanosalsum natronophilum sp. nov., and Methanocalculus alkaliphilus sp. nov., haloalkaliphilic methanogens from hypersaline soda lakes.

    abstract::Two groups of haloalkaliphilic methanogenic archaea were dominating in enrichments from hypersaline soda lake sediments at pH 10. At moderate salt concentrations with formate or H2 as electron donor, methanogens belonging to the genus Methanocalculus were enriched, while at high salt concentrations with methylated sub...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000488

    authors: Sorokin DY,Abbas B,Merkel AY,Rijpstra WIC,Damsté JSS,Sukhacheva MV,van Loosdrecht MCM

    更新日期:2015-10-01 00:00:00

  • Flavobacterium beibuense sp. nov., isolated from marine sediment.

    abstract::A taxonomic study was carried out on strain F44-8(T), which was isolated from a crude-oil-degrading consortium, enriched from marine sediment of the Beibu Gulf, PR China. The 16S rRNA gene sequence of strain F44-8(T) showed highest similarities to those of Flavobacterium frigoris LMG 21922(T) (93.3 %), Flavobacterium ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.018846-0

    authors: Fu Y,Tang X,Lai Q,Zhang C,Zhong H,Li W,Liu Y,Chen L,Sun F,Shao Z

    更新日期:2011-01-01 00:00:00

  • Reclassification of [Cytophaga] marinoflava Reichenbach 1989 as Leeuwenhoekiella marinoflava gen. nov., comb. nov. and description of Leeuwenhoekiella aequorea sp. nov.

    abstract::Five heterotrophic, aerobic, halotolerant and pigmented bacterial strains with gliding motility were isolated from Antarctic sea water; one other isolate was collected from the sea urchin Strongylocentrotus intermedius in the Gulf of Peter the Great in the Sea of Japan. 16S rRNA gene sequence analysis indicated that t...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63410-0

    authors: Nedashkovskaya OI,Vancanneyt M,Dawyndt P,Engelbeen K,Vandemeulebroecke K,Cleenwerck I,Hoste B,Mergaert J,Tan TL,Frolova GM,Mikhailov VV,Swings J

    更新日期:2005-05-01 00:00:00

  • Bensingtonia rectispora sp. nov. and Bensingtonia bomiensis sp. nov., ballistoconidium-forming yeast species from Tibetan plant leaves.

    abstract::Five yeast strains isolated from plant leaves collected in south-east Tibet formed cream to brownish colonies and produced asymmetrical ballistoconidia and CoQ-9 as the major ubiquinone. Sequence analysis of the 26S rRNA D1/D2 domain and the internal transcribed spacer region indicated that these strains represented t...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.038117-0

    authors: Wang QM,Boekhout T,Bai FY

    更新日期:2012-08-01 00:00:00

  • Haloprofundus marisrubri gen. nov., sp. nov., an extremely halophilic archaeon isolated from a brine-seawater interface.

    abstract::We isolated a Gram-stain-negative, pink-pigmented, motile, pleomorphic, extremely halophilic archaeon from the brine-seawater interface of Discovery Deep in the Saudi Arabian Red Sea. This strain, designated SB9T, was capable of growth within a wide range of temperatures and salinity, but required MgCl2. Cells lysed i...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001559

    authors: Zhang G,Gu J,Zhang R,Rashid M,Haroon MF,Xun W,Ruan Z,Dong X,Stingl U

    更新日期:2017-01-01 00:00:00

  • Phylogenomics and protein signatures elucidating the evolutionary relationships among the Gammaproteobacteria.

    abstract::The class Gammaproteobacteria, which forms one of the largest groups within bacteria, is currently distinguished from other bacteria solely on the basis of its branching in phylogenetic trees. No molecular or biochemical characteristic is known that is unique to the class Gammaproteobacteria or its different subgroups...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.002741-0

    authors: Gao B,Mohan R,Gupta RS

    更新日期:2009-02-01 00:00:00

  • Chitinibacter fontanus sp. nov., isolated from a spring.

    abstract::A bacterial strain, designated STM-7T, was isolated from a spring in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain STM-7T were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate-accumulating, motile by a single polar flagellum, rod-shaped, surrounded by a thick capsule and formed ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001345

    authors: Sheu SY,Li YS,Young CC,Chen WM

    更新日期:2016-10-01 00:00:00

  • Pseudomonas lactis sp. nov. and Pseudomonas paralactis sp. nov., isolated from bovine raw milk.

    abstract::Five strains, designated WS 4672T, WS 4998, WS 4992T, WS 4997 and WS 5000, isolated from bovine raw milk formed two individual groups in a phylogenetic analysis. The most similar species on the basis of 16S rRNA gene sequences were Pseudomonas azotoformans IAM 1603T, Pseudomonas gessardii CIP 105469T and Pseudomonas l...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001836

    authors: von Neubeck M,Huptas C,Glück C,Krewinkel M,Stoeckel M,Stressler T,Fischer L,Hinrichs J,Scherer S,Wenning M

    更新日期:2017-06-01 00:00:00

  • Paenibacillus physcomitrellae sp. nov., isolated from the moss Physcomitrella patens.

    abstract::A Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain XBT, was isolated from Physcomitrella patens growing in Beijing, China. The isolate was identified as a member of the genus Paenibacillus based on phenotypic characteristics and phylogenetic inferences. The novel strain was spor...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000428

    authors: Zhou X,Nan Guo G,Qi Wang L,Lan Bai S,Li Li C,Yu R,Hong Li Y

    更新日期:2015-10-01 00:00:00

  • New approach to phylogenetic analysis of the genus Bifidobacterium based on partial HSP60 gene sequences.

    abstract::The partial 60 kDa heat-shock protein (HSP60) genes of 36 Bifidobacterium strains representing 30 different Bifidobacterium species and subspecies and of the type strain of Gardnerella vaginalis were cloned and sequenced using a pair of universal degenerate HSP60 PCR primers. The HSP60 DNA sequence similarities were d...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-51-5-1633

    authors: Jian W,Zhu L,Dong X

    更新日期:2001-09-01 00:00:00

  • Sulfurivermis fontis gen. nov., sp. nov., a sulfur-oxidizing autotroph, and proposal of Thioprofundaceae fam. nov.

    abstract::A novel Gram-stain-negative, chemolithoautotrophic sulfur oxidizer, strain JG42T, was isolated from a hot spring microbial mat. As an electron donor for autotrophic growth, strain JG42T utilized sulfide, thiosulfate, tetrathionate and elemental sulfur. Cells of strain JG42T were oxidase-positive and catalase-negative....

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002137

    authors: Kojima H,Watanabe M,Fukui M

    更新日期:2017-09-01 00:00:00

  • Pelagirhabdus alkalitolerans gen. nov., sp. nov., an alkali-tolerant and thermotolerant bacterium isolated from beach sediment, and reclassification of Amphibacillus fermentum as Pelagirhabdus fermentum comb. nov.

    abstract::A novel bacterial strain, designated S5T, was isolated from Pingaleshwar beach, in India. Cells were Gram-stain-positive, rod-shaped, non-motile and non-endospore-forming. Based on 16S rRNA gene sequence analysis, the strain was identified as belonging to the class Firmibacteria and was related most closely to Amphiba...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000678

    authors: Sultanpuram VR,Mothe T,Chintalapati S,Chintalapati VR

    更新日期:2016-01-01 00:00:00

  • Micromonospora equina sp. nov., isolated from soil from a racecourse.

    abstract::Two actinomycete strains were isolated from within the fynbos-rich area surrounded by the horseracing track at Kenilworth Racecourse in Cape Town, South Africa. Rapid molecular identification indicated that the isolates belonged to the family Micromonosporaceae. Based on 16S rRNA gene sequence blast analysis, the isol...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.042929-0

    authors: Everest GJ,Meyers PR

    更新日期:2013-03-01 00:00:00

  • Caldanaerovirga acetigignens gen. nov., sp. nov., an anaerobic xylanolytic, alkalithermophilic bacterium isolated from Trego Hot Spring, Nevada, USA.

    abstract::An anaerobic thermophilic bacterium, designated strain JW/SA-NV4(T), was isolated from a xylan-supplemented enrichment culture from Trego hot spring located within the Black Rock Desert (NV, USA). Cells were generally straight or slightly bent rod-shaped, 0.4-0.8 microm in width and 3-6 microm in length during exponen...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.005207-0

    authors: Wagner ID,Ahmed S,Zhao W,Zhang CL,Romanek CS,Rohde M,Wiegel J

    更新日期:2009-11-01 00:00:00

  • Campylobacter armoricus sp. nov., a novel member of the Campylobacter lari group isolated from surface water and stools from humans with enteric infection.

    abstract::During a study on the prevalence and diversity of members of the genus Campylobacter in a shellfish-harvesting area and its catchment in Brittany, France, six urease-positive isolates of members of the genus Campylobacter were recovered from surface water samples, as well as three isolates from stools of humans displa...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003836

    authors: Boukerb AM,Penny C,Serghine J,Walczak C,Cauchie HM,Miller WG,Losch S,Ragimbeau C,Mossong J,Mégraud F,Lehours P,Bénéjat L,Gourmelon M

    更新日期:2019-12-01 00:00:00

  • Chryseobacterium gregarium sp. nov., isolated from decaying plant material.

    abstract::In this study, strain P 461/12T, isolated from decaying plant material after mulching a pasture, was shown to represent a novel species of the genus Chryseobacterium by means of a polyphasic approach. The closest phylogenetic neighbours to the novel strain, as determined by 16S rRNA gene sequence analysis, were Chryse...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65544-0

    authors: Behrendt U,Ulrich A,Schumann P

    更新日期:2008-05-01 00:00:00