New approach to phylogenetic analysis of the genus Bifidobacterium based on partial HSP60 gene sequences.

Abstract:

:The partial 60 kDa heat-shock protein (HSP60) genes of 36 Bifidobacterium strains representing 30 different Bifidobacterium species and subspecies and of the type strain of Gardnerella vaginalis were cloned and sequenced using a pair of universal degenerate HSP60 PCR primers. The HSP60 DNA sequence similarities were determined for the taxa at various ranks as follows: 99.4-100% within the same species, 96% at the subspecies level, and 73-96% (mean 85%) at the interspecies level (and 98% in the case of two groups of closely related species, Bifidobacterium animalis and Bifidobacterium lactis, Bifidobacterium infantis, Bifidobacterium longum and Bifidobacterium suis, whose 165 rRNA sequence similarities are all above 99%). The HSP60 DNA sequence similarities between different Bifidobacterium species and G. vaginalis, a closely related bacterium according to 16S rRNA analysis, ranged from 71 to 79% (mean 75%). Although the topology of the phylogenetic tree constructed using the HSP60 sequences determined was basically similar to that for 16S rRNA, it seemed to be more clear-cut for species delineation, and the clustering was better correlated with the DNA base composition (mol% G+C) than that of the 16S rRNA tree. In the HSP60 phylogenetic tree, all of the high-G+C (55-67 mol%) bifidobacteria were grouped into one cluster, whereas the low-G+C species Bifidobacterium inopinatum (45 mol %) formed a separate cluster with G. vaginalis (42 mol%) and Bifidobacterium denticolens (55 mol%); a Bifidobacterium species of intermediate G+C content formed another cluster between the two. This study demonstrates that the highly conserved and ubiquitous HSP60 gene is an accurate and convenient tool for phylogenetic analysis of the genus Bifidobacterium.

authors

Jian W,Zhu L,Dong X

doi

10.1099/00207713-51-5-1633

keywords:

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

1633-1638

issue

Pt 5

eissn

1466-5026

issn

1466-5034

journal_volume

51

pub_type

杂志文章
  • Rhizobium anhuiense sp. nov., isolated from effective nodules of Vicia faba and Pisum sativum.

    abstract::Four rhizobia-like strains, isolated from root nodules of Pisum sativum and Vicia faba grown in Anhui and Jiangxi Provinces of China, were grouped into the genus Rhizobium but were distinct from all recognized species of the genus Rhizobium by phylogenetic analysis of 16S rRNA and housekeeping genes. The combined sequ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.000365

    authors: Zhang YJ,Zheng WT,Everall I,Young JPW,Zhang XX,Tian CF,Sui XH,Wang ET,Chen WX

    更新日期:2015-09-01 00:00:00

  • Mycobacterium lacus sp. nov., a novel slowly growing, non-chromogenic clinical isolate.

    abstract::A strain of a novel non-chromogenic mycobacterium was isolated from synovial tissue from a 68-year-old female with bursitis of her right elbow. The slowly growing strain had a unique PCR-restriction enzyme analysis (PRA) profile of the hsp65 gene and 16S rRNA gene sequence in comparison with other mycobacterium specie...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-52-6-2135

    authors: Turenne C,Chedore P,Wolfe J,Jamieson F,Broukhanski G,May K,Kabani A

    更新日期:2002-11-01 00:00:00

  • Salegentibacter mishustinae sp. nov., isolated from the sea urchin Strongylocentrotus intermedius.

    abstract::A bacterial strain, designated KMM 6049T, was isolated from the sea urchin Strongylocentrotus intermedius inhabiting the Sea of Japan. The bacterium studied was strictly aerobic, heterotrophic, yellow-pigmented, non-motile, Gram-negative and oxidase-, catalase-, beta-galactosidase- and alkaline phosphatase-positive. 1...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63297-0

    authors: Nedashkovskaya OI,Kim SB,Lysenko AM,Mikhailov VV,Bae KS,Kim IS

    更新日期:2005-01-01 00:00:00

  • Amycolatopsis helveola sp. nov. and Amycolatopsis pigmentata sp. nov., isolated from soil.

    abstract::Three short spore chain-forming actinomycete strains were isolated from soil samples collected from subtropical islands in Japan. The cell-wall peptidoglycan of these strains contained meso-diaminopimelic acid (meso-A(2)pm), glutamic acid and alanine. The major isoprenoid quinone was MK-9(H(4)), iso-C(16 : 0) and 2-OH...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.017871-0

    authors: Tamura T,Ishida Y,Otoguro M,Suzuki KI

    更新日期:2010-11-01 00:00:00

  • Polyphasic characterization of xanthomonads pathogenic to members of the Anacardiaceae and their relatedness to species of Xanthomonas.

    abstract::We have used amplified fragment length polymorphism (AFLP), multilocus sequence analysis (MLSA) and DNA-DNA hybridization for genotypic classification of Xanthomonas pathovars associated with the plant family Anacardiaceae. AFLP and MLSA results showed congruent phylogenetic relationships of the pathovar mangiferaeind...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65453-0

    authors: Ah-You N,Gagnevin L,Grimont PA,Brisse S,Nesme X,Chiroleu F,Bui Thi Ngoc L,Jouen E,Lefeuvre P,Vernière C,Pruvost O

    更新日期:2009-02-01 00:00:00

  • Dactylosporangium siamense sp. nov., isolated from soil.

    abstract::A novel actinomycete strain, designed MW4-36(T), was isolated from tropical forest soil in Nakhon Sawan Province, Thailand. Morphological and chemotaxonomic characteristics of this strain clearly demonstrated that it belongs to the genus Dactylosporangium. The strain formed finger-shaped sporangia on short sporangioph...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.052092-0

    authors: Thawai C,Suriyachadkun C

    更新日期:2013-11-01 00:00:00

  • Candida ecuadorensis sp. nov., an ascomycetous yeast species found in two separate regions of Ecuador.

    abstract::In the course of an on-going study aimed at cataloguing the natural yeast biodiversity found in Ecuador, two strains (CLQCA 13-025 and CLQCA 20-004(T)) were isolated from samples of cow manure and rotten wood collected in two separate provinces of the country (Orellana and Bolívar). These strains were found to represe...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.047555-0

    authors: James SA,Carvajal Barriga EJ,Barahona PP,Cross K,Bond CJ,Roberts IN

    更新日期:2013-01-01 00:00:00

  • Aneurinibacillus soli sp. nov., isolated from mountain soil.

    abstract::A novel bacterial strain designated CB4(T) was isolated from soil from the Hallasan, Jeju, Korea. Strain CB4(T) was found to be strictly aerobic, Gram-stain-positive, rod-shaped, motile and formed creamy greyish colonies on nutrient agar. The major fatty acids were identified as iso-C(15:0) and iso-C(16:0), and the pr...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.066779-0

    authors: Lee KC,Kim KK,Eom MK,Kim JS,Kim DS,Ko SH,Lee JS

    更新日期:2014-11-01 00:00:00

  • Streptomyces venetus sp. nov., an actinomycete with a blue aerial mycelium.

    abstract::A Gram-positive bacterium, designated CMU-AB225T, was isolated from rhizosphere soil of an oil palm (Elaeis guineensis). The strain exhibited a blue aerial spore mass and a light cream to moderate yellow substrate mycelium and formed chains of spiny spores. Whole-cell hydrolysates consisted of ll-diaminopimelic acid, ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002995

    authors: Sujarit K,Kudo T,Ohkuma M,Pathom-Aree W,Lumyong S

    更新日期:2018-10-01 00:00:00

  • Rubrivirga profundi sp. nov., isolated from deep-sea water, and emended description of the genus Rubrivirga.

    abstract::A Gram-staining-negative, rod-shaped, facultatively anaerobic, non-motile and pale-red-pigmented bacterium, designated SAORIC-476T, was isolated from deep-sea water from the Pacific Ocean. 16S rRNA gene sequence analyses showed that strain SAORIC-476T was most closely related to Rubrivirga marinaSAORIC-28T (96.8 % sim...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.001182

    authors: Song J,Joung Y,Park S,Cho JC,Kogure K

    更新日期:2016-09-01 00:00:00

  • Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea.

    abstract::Two Gram-positive or Gram-variable, endospore-forming, moderately halophilic rods (strains TF-11(T) and TF-12(T)) were isolated from a tidal flat of the Yellow Sea in Korea and were subjected to a polyphasic taxonomic study. Strains TF-11(T) and TF-12(T) had cell-wall peptidoglycan based on meso-diaminopimelic acid. T...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.02365-0

    authors: Yoon JH,Kim IG,Kang KH,Oh TK,Park YH

    更新日期:2003-09-01 00:00:00

  • Elstera litoralis gen. nov., sp. nov., isolated from stone biofilms of Lake Constance, Germany.

    abstract::An alphaproteobacterium, strain Dia-1(T), was isolated from algae-dominated biofilms on stones from the littoral zone of Lake Constance, Germany. This bacterium was isolated after initial enrichment in spent medium obtained after growth of a diatom culture. Numerous sugars and some organic acids and alcohols served as...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.026609-0

    authors: Rahalkar M,Bahulikar RA,Deutzmann JS,Kroth PG,Schink B

    更新日期:2012-08-01 00:00:00

  • Paenibacillus assamensis sp. nov., a novel bacterium isolated from a warm spring in Assam, India.

    abstract::A polyphasic approach was used to characterize a bacterium, GPTSA 11(T), isolated from a warm spring located in a reserve forest in Assam, India. The cells are Gram-variable, strictly aerobic, sporulating motile rods. The major fatty acids of the strain are C(15 : 0) anteiso (48.42 %), C(16 : 0) iso (11.59 %), C(16 : ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.63846-0

    authors: Saha P,Mondal AK,Mayilraj S,Krishnamurthi S,Bhattacharya A,Chakrabarti T

    更新日期:2005-11-01 00:00:00

  • Pyruvatibacter mobilis gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122.

    abstract::A Gram-stain-negative, aerobic bacterium, designated strain GYP-11T, was isolated from the culture broth of a marine microalga, Picochloruma sp. 122. Cells were dimorphic rods; free living cells were motile by means of a single polar flagellum, and star-shaped-aggregate-forming cells were attached with stalks and non-...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.000692

    authors: Wang G,Tang M,Wu H,Dai S,Li T,Chen C,He H,Fan J,Xiang W,Li X

    更新日期:2016-01-01 00:00:00

  • Spongiibacter marinus gen. nov., sp. nov., a halophilic marine bacterium isolated from the boreal sponge Haliclona sp. 1.

    abstract::Strain HAL40b(T) was isolated from the marine sponge Haliclona sp. 1 collected at the Sula Ridge off the Norwegian coast and characterized by physiological, biochemical and phylogenetic analyses. The isolate was a small rod with a polar flagellum. It was aerobic, Gram-negative and oxidase- and catalase-positive. Optim...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.65438-0

    authors: Graeber I,Kaesler I,Borchert MS,Dieckmann R,Pape T,Lurz R,Nielsen P,von Döhren H,Michaelis W,Szewzyk U

    更新日期:2008-03-01 00:00:00

  • Oceanipulchritudo coccoides gen. nov., sp. nov., isolated from marine sediment within the family Puniceicoccaceae.

    abstract::A Gram-stain-negative, aerobic coccus, designated CK1056T, was isolated from coastal sediment of Xiaoshi Island, Weihai, PR China. Strain CK1056T was found to grow at 15-37 °C (optimum, 30 °C), with 0.5-6.5 % (w/v) NaCl (optimum, 3.5 %) and displayed alkaliphilic growth within the pH range of pH 6.5-10.0 (optimum, pH ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004458

    authors: Feng X,Zou QH,Zhang XY,Ye MQ,Du ZJ

    更新日期:2020-11-01 00:00:00

  • Proposal to transfer Actinomadura carminata to a new subspecies of the genus Nonomuraea as Nonomuraea roseoviolacea subsp. carminata comb. nov.

    abstract::An anthracycline-producing actinomycete (strain SF2303) was compared with two other anthracycline producers, Actinomadura carminata IFO 15903T and Nonomuraea roseoviolacea IFO 14098T, using morphological, physiological, chemotaxonomic and molecular-genetic criteria. The morphological and cultural characteristics of th...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-51-3-881

    authors: Gyobu Y,Miyadoh S

    更新日期:2001-05-01 00:00:00

  • Microbacterium excoecariae sp. nov., a novel endophytic actinobacterium isolated from bark of Excoecaria agallocha Linn.

    abstract::A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China. Cells were short rods. Colonies were light yellow, circular and had entire margins. Strain CBS5P-1T grew at 10-37 °C (optimum, 30 °C...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.004523

    authors: Chen MS,Li F,Yan XR,Tuo L

    更新日期:2020-12-01 00:00:00

  • Lactobacillus silagei sp. nov., isolated from orchardgrass silage.

    abstract::A Gram-reaction-positive, facultatively anaerobic, non-spore-forming and catalase-negative rod-shaped bacterial strain, designated IWT126(T), was isolated from orchardgrass (Dactylis glomerata L.) silage preserved in Hachimantai, Iwate, Japan. The isolate showed growth at 15-45 °C, pH 3.5-7.5 and with 4.0 % (w/v) NaCl...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.053124-0

    authors: Tohno M,Kitahara M,Irisawa T,Masuda T,Uegaki R,Ohkuma M,Tajima K

    更新日期:2013-12-01 00:00:00

  • Vibrio ouci sp. nov. and Vibrio aquaticus sp. nov., two marine bacteria isolated from the East China Sea.

    abstract::Two Gram-stain-negative, catalase- and oxidase-positive, facultative anaerobic and rod-shaped motile bacteria, designated strains BEI176T and BEI207T, were isolated from seawater collected in the East China Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains BEI176T and BEI207T belonged to ...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003732

    authors: Li B,Li Y,Liu R,Xue C,Zhu X,Tian X,Wang X,Liang J,Zheng Y,Zhang XH

    更新日期:2020-01-01 00:00:00

  • Histidinibacterium lentulum gen. nov., sp. nov., a marine bacterium from the culture broth of marine microalga Picochlorum sp. 122.

    abstract::A Gram-stain-negative, non-spore-forming, aerobic, motile, ovoid or short rod shaped bacterium, designed strain B17T, was isolated from the culture broth of Picochlorum sp. 122. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain B17T forms a stable cluster with Oceanicolagranulosus MCCC 1A105...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003225

    authors: Wang G,Xu S,Su H,Chen B,Liang J,Huang W,Wang Y,Yu K

    更新日期:2019-02-01 00:00:00

  • Erythrobacter zhengii sp. nov., a bacterium isolated from deep-sea sediment.

    abstract::A Gram-stain-negative, strictly aerobic, rod-shaped bacterium, designated V18T, was isolated from a deep-sea sediment sample collected from the Pacific Ocean and subjected to a polyphasic taxonomic investigation. Cells of strain V18T grew in medium containing 0-10.0 % (w/v) NaCl (optimum 1.0 %), at pH 5.5-9.0 (optimum...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.003136

    authors: Fang C,Wu YH,Sun C,Wang H,Cheng H,Meng FX,Wang CS,Xu XW

    更新日期:2019-01-01 00:00:00

  • Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov., and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water.

    abstract::The taxonomic status of four strains from several mineral water sources in Taejon, Korea, was examined. The DNA base compositions of these strains ranged from 62 to 66 mol %. All of the strains contained sphingolipids and ubiquinone 10 as the main respiratory quinone. The cellular fatty acids of these strains included...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-51-4-1491

    authors: Lee JS,Shin YK,Yoon JH,Takeuchi M,Pyun YR,Park YH

    更新日期:2001-07-01 00:00:00

  • Echinicola sediminis sp. nov., a marine bacterium isolated from coastal sediment.

    abstract::A novel Gram-stain-negative, rod-shaped, gliding bacterium, designated strain 001-Na2T, was isolated from coastal sediment of the Yellow Sea in Sindu-ri, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. Colonies were dark salmon-coloured, circular, and pulvinate with entire margins. 16S r...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002118

    authors: Lee DW,Lee AH,Lee H,Kim JJ,Khim JS,Yim UH,Kim BS

    更新日期:2017-09-01 00:00:00

  • Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (Populus deltoides x nigra DN34).

    abstract::A pink-pigmented, aerobic, facultatively methylotrophic bacterium, strain BJ001T, was isolated from internal poplar tissues (Populus deltoidesxnigra DN34) and identified as a member of the genus Methylobacterium. Phylogenetic analyses showed that strain BJ001T is related to Methylobacterium thiocyanatum, Methylobacter...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.02796-0

    authors: Van Aken B,Peres CM,Doty SL,Yoon JM,Schnoor JL

    更新日期:2004-07-01 00:00:00

  • Asaccharobacter celatus gen. nov., sp. nov., isolated from rat caecum.

    abstract::An obligately anaerobic and equol-producing bacterium, designated strain do03T, was isolated from the caecal content of a rat. Cells were Gram-positive, non-spore-forming rods. The results from a phylogenetic analysis based on 16S rRNA gene sequences showed that strain do03T formed a separate line of descent in the ph...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.64894-0

    authors: Minamida K,Ota K,Nishimukai M,Tanaka M,Abe A,Sone T,Tomita F,Hara H,Asano K

    更新日期:2008-05-01 00:00:00

  • Flavobacterium amniphilum sp. nov., isolated from a stream.

    abstract::Strain KYPY10T, isolated from a water sample taken from the Funglin Stream in Taiwan, was characterized using a polyphasic taxonomic approach. Cells of strain KYPY10T were Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped surrounded by a thick capsule, and formed light-yellow colonies. Growth occurr...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002443

    authors: Sheu SY,Su CL,Kwon SW,Chen WM

    更新日期:2017-12-01 00:00:00

  • Phyllobacterium sophorae sp. nov., a symbiotic bacterium isolated from root nodules of Sophora flavescens.

    abstract::Two novel Gram-stain-negative strains (CCBAU 03422(T) and CCBAU 03415) isolated from root nodules of Sophora flavescens were classified phylogenetically into the genus Phyllobacterium based on the comparative analysis of 16S rRNA and atpD genes. They showed 99.8 % rRNA gene sequence similarities to Phyllobacterium bra...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijs.0.067017-0

    authors: Jiao YS,Yan H,Ji ZJ,Liu YH,Sui XH,Zhang XX,Wang ET,Chen WX,Chen WF

    更新日期:2015-02-01 00:00:00

  • Kuraishia mediterranea sp. nov., a methanol-assimilating yeast species from olive oil and its sediment.

    abstract::Six yeast strains isolated from olive oil sediments and spoiled olive oils originating from Slovenia and Portugal, respectively, proved to represent an undescribed yeast species based on DNA sequence comparisons. The analysis of gene sequences for internal transcribed spacer regions and the large subunit rRNA gene D1/...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/ijsem.0.002392

    authors: Čadež N,Dlauchy D,Tóbiás A,Péter G

    更新日期:2017-11-01 00:00:00

  • Arthrobacter nasiphocae sp. nov., from the common seal (Phoca vitulina).

    abstract::An unknown gram-positive, catalase-positive, strictly aerobic, rod-shaped bacterium was isolated from the nasal cavities of two common seals. Chemical analysis revealed the presence in the bacterium of a hitherto unknown cell-wall murein [type: L-Lys-L-Ala2-Gly(2-3)-L-Ala (Gly)]. Comparative 16S rRNA gene sequencing s...

    journal_title:International journal of systematic and evolutionary microbiology

    pub_type: 杂志文章

    doi:10.1099/00207713-52-2-569

    authors: Collins MD,Hoyles L,Foster G,Falsen E,Weiss N

    更新日期:2002-03-01 00:00:00