Abstract：
:We describe a new method to detect trace levels of ptaquiloside (Pta), a major carcinogen of bracken fern in biological samples such as milk from farm animals. The method involves the absorption of analyte on carbograph followed by elution with solvents mixtures. The unstable analyte is then converted into Br-Pt (II), which is specific for Pta, as it is not a natural decay product of the glycoside in aqueous media. An internal standard, the Br-pterosine-d2, prepared in our laboratories has been used. Detection and quantification are possible with gas chromatography/mass spectrometry (GC/MS) in single ion monitoring mode (SIM). The detectable amount is in the range of ppb. The method allowed us to detect Pta not only in the milk from bracken fern-poisoned cattle but also, for the first time, in the milk from healthy farm animals such as sheep, goat, horse, and donkey mares.
journal_name
Food Chemjournal_title
Food chemistryauthors
Francesco B,Giorgio B,Rosario N,Saverio RF,Francesco G,Romano M,Adriano S,Cinzia R,Antonio T,Franco R,Valeria R,Sante Rdoi
10.1016/j.foodchem.2010.05.059subject
Has Abstractpub_date
2011-01-15 00:00:00pages
660-665issue
2eissn
0308-8146issn
1873-7072pii
S0308-8146(10)00633-3journal_volume
124pub_type
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