Abstract:
:The efficacy of different buffers in extracting peanut from a solid model food incurred with peanut and subjected to processing was evaluated using two commercial ELISA kits: Veratox® for peanut allergen and peanut ELISA from Morinaga. Average percentage recoveries of peanut from unprocessed samples using the kit supplied buffers were 46 ± 5 and 28 ± 2 with the Veratox and Morinaga kits, respectively. However, Na2CO3, pH 9.6 and PBS containing 1 M GuHCl recovered 65% ± 4% and 77% ± 10% of peanut, respectively from unprocessed samples with the Veratox kit. These two buffers also performed better than the Veratox buffer with fried, high pressure processed, and baked samples. PBS containing SDS and β-ME, performed significantly better than the Morinaga buffer in recovering peanut from unprocessed, boiled and fried samples. Thus, the use of alternative extraction buffers provides better recovery of peanut residues from a processed solid food matrix.
journal_name
Food Chemjournal_title
Food chemistryauthors
Jayasena S,Wijeratne SSK,Taylor SL,Baumert JLdoi
10.1016/j.foodchem.2018.11.123subject
Has Abstractpub_date
2019-04-25 00:00:00pages
832-840eissn
0308-8146issn
1873-7072pii
S0308-8146(18)32070-3journal_volume
278pub_type
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