Towards PacBio-based pan-eukaryote metabarcoding using full-length ITS sequences.

Abstract:

:Development of high-throughput sequencing techniques has greatly benefited our understanding about microbial ecology, yet the methods producing short reads suffer from species-level resolution and uncertainty of identification. Here, we optimize Pacific Biosciences-based metabarcoding protocols covering the internal transcribed spacer (ITS region) and partial small subunit of the rRNA gene for species-level identification of all eukaryotes, with a specific focus on Fungi (including Glomeromycota) and Stramenopila (particularly Oomycota). Based on tests on composite soil samples and mock communities, we propose best suitable degenerate primers, ITS9munngs + ITS4ngsUni for eukaryotes and selected groups therein and discuss the pros and cons of long read-based identification of eukaryotes.

journal_name

Environ Microbiol Rep

authors

Tedersoo L,Anslan S

doi

10.1111/1758-2229.12776

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

659-668

issue

5

issn

1758-2229

journal_volume

11

pub_type

杂志文章
  • Cell-specific CO2 fixation rates of two distinct groups of plastidic protists in the Atlantic Ocean remain unchanged after nutrient addition.

    abstract::To assess the role of open-ocean ecosystems in global CO2 fixation, we investigated how picophytoplankton, which dominate primary production, responded to episodic increases in nutrient availability. Previous experiments have shown nitrogen alone, or in combination with phosphorus or iron, to be the proximate limiting...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12228

    authors: Grob C,Jardillier L,Hartmann M,Ostrowski M,Zubkov MV,Scanlan DJ

    更新日期:2015-04-01 00:00:00

  • Commensal Escherichia coli strains in Guiana reveal a high genetic diversity with host-dependant population structure.

    abstract::We undertook a large-scale epidemiological survey of commensal Escherichia coli in Trois-Sauts, an isolated village located in the south of French Guiana where human population exchanges are restricted and source of antibiotics controlled. Stools from 162 Wayampi Amerindians and rectal swabs from 33 human associated a...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2012.00374.x

    authors: Lescat M,Clermont O,Woerther PL,Glodt J,Dion S,Skurnik D,Djossou F,Dupont C,Perroz G,Picard B,Catzeflis F,Andremont A,Denamur E

    更新日期:2013-02-01 00:00:00

  • Water-column stratification governs the community structure of subtropical marine picophytoplankton.

    abstract::The increase in the areal extent of the subtropical gyres over the past decade has been attributed to a global tendency towards increased water-column stratification. Here, we examine how vertical stratification governs the community structure of the picophytoplankton that dominate these vast marine ecosystems. We ana...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2011.00241.x

    authors: Bouman HA,Ulloa O,Barlow R,Li WK,Platt T,Zwirglmaier K,Scanlan DJ,Sathyendranath S

    更新日期:2011-08-01 00:00:00

  • KaiC family proteins integratively control temperature-dependent UV resistance in Methylobacterium extorquens AM1.

    abstract::KaiC protein is the pivotal component of the circadian clock in cyanobacteria. While KaiC family proteins are well-conserved throughout divergent phylogenetic lineages, studies of the physiological roles of KaiC proteins from other microorganisms have been limited. We examined the role of the KaiC proteins, KaiC1 and ...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12662

    authors: Iguchi H,Yoshida Y,Fujisawa K,Taga H,Yurimoto H,Oyama T,Sakai Y

    更新日期:2018-12-01 00:00:00

  • Chemotype diversity in Planktothrix rubescens (cyanobacteria) populations is correlated to lake depth.

    abstract::The cyanobacterial species Planktothrix rubescens is known to preferably inhabit deep, stratified, oligo- to mesotrophic lakes. It is also known for the production of diverse bioactive peptides, including the hepatotoxic microcystins. A number of studies showed that P. rubescens populations generally consist of multip...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12519

    authors: Haruštiaková D,Welker M

    更新日期:2017-04-01 00:00:00

  • Nitrate addition has minimal short-term impacts on greenland ice sheet supraglacial prokaryotes.

    abstract::Tropospheric nitrate levels are predicted to increase throughout the 21st century, with potential effects on terrestrial ecosystems, including the Greenland ice sheet (GrIS). This study considers the impacts of elevated nitrate concentrations on the abundance and composition of dominant bulk and active prokaryotic com...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12510

    authors: Cameron KA,Stibal M,Chrismas N,Box J,Jacobsen CS

    更新日期:2017-04-01 00:00:00

  • The versatile in situ gene expression of an Epsilonproteobacteria-dominated biofilm from a hydrothermal chimney.

    abstract::The Epsilonproteobacteria, including members of the genus Sulfurovum, are regarded as important primary producers in hydrothermal systems. However, their in situ gene expression in this habitat has so far not been investigated. We report a metatranscriptomic analysis of a Sulfurovum-dominated biofilm from one of the c...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12016

    authors: Dahle H,Roalkvam I,Thorseth IH,Pedersen RB,Steen IH

    更新日期:2013-04-01 00:00:00

  • Role of GbpA protein, an important virulence-related colonization factor, for Vibrio cholerae's survival in the aquatic environment.

    abstract::Vibrio cholerae N-acetyl glucosamine-binding protein A (GbpA) is a chitin binding protein and a virulence factor involved in the colonization of human intestine. We investigated the distribution and genetic variations of gbpA in 488 V. cholerae strains of environmental and clinical origin, belonging to different serog...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2012.00356.x

    authors: Stauder M,Huq A,Pezzati E,Grim CJ,Ramoino P,Pane L,Colwell RR,Pruzzo C,Vezzulli L

    更新日期:2012-08-01 00:00:00

  • Reductive dechlorination of 2-chlorophenol by Anaeromyxobacter dehalogenans with an electrode serving as the electron donor.

    abstract::Electrodes poised at potentials low enough to serve as an electron donor for microbial respiration, but high enough to avoid the production of hydrogen, have been proposed as an alternative to the use of soluble electron donors for stimulating the bioremediation of chlorinated contaminants and/or metals. However, this...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2009.00118.x

    authors: Strycharz SM,Gannon SM,Boles AR,Franks AE,Nevin KP,Lovley DR

    更新日期:2010-04-01 00:00:00

  • Low seasonal variability in community composition of sediment bacteria in large and shallow lake.

    abstract::The community composition of bacteria with highly dynamic abundance and activity was observed to be with low variability in a shallow lake sediment with frequent physical disturbance. This suggests that physical disturbance did not create more niches and did not lead to highly variable bacterial community. The major p...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2010.00221.x

    authors: Tšertova N,Kisand A,Tammert H,Kisand V

    更新日期:2011-04-01 00:00:00

  • Yeasts from macroalgae and lichens that inhabit the South Shetland Islands, Antarctica.

    abstract::Antarctic terrestrial ecosystems are largely dominated by lichens, while shallow coastal environments are mainly covered by macroalgae. The aim of this study was to isolate and to evaluate the diversity of yeasts in different species of macroalgae and lichens collected in South Shetland Islands, Antarctica. A total of...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12452

    authors: Duarte AWF,Passarini MRZ,Delforno TP,Pellizzari FM,Cipro CVZ,Montone RC,Petry MV,Putzke J,Rosa LH,Sette LD

    更新日期:2016-10-01 00:00:00

  • Genomic distribution of B-vitamin auxotrophy and uptake transporters in environmental bacteria from the Chloroflexi phylum.

    abstract::Bacteria from the Chloroflexi phylum are dominant members of phototrophic microbial mat communities in terrestrial thermal environments. Vitamins of B group are key intermediates (precursors) in the biosynthesis of indispensable enzyme cofactors driving numerous metabolic processes in all forms of life. A genomics-bas...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12227

    authors: Rodionova IA,Li X,Plymale AE,Motamedchaboki K,Konopka AE,Romine MF,Fredrickson JK,Osterman AL,Rodionov DA

    更新日期:2015-04-01 00:00:00

  • Targeted and shotgun metagenomic approaches provide different descriptions of dryland soil microbial communities in a manipulated field study.

    abstract::The extent to which different sequence-based approaches describe environmental microbial communities in comparative studies is an important consideration when deriving inferences from ecological studies. The ability of a targeted metagenomic approach [small subunit (SSU) rRNA pyrosequencing] and shotgun metagenome app...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2012.00328.x

    authors: Steven B,Gallegos-Graves LV,Starkenburg SR,Chain PS,Kuske CR

    更新日期:2012-04-01 00:00:00

  • Genetic dissection of a motility-associated c-di-GMP signalling protein of Pseudomonas putida.

    abstract::Lack of the Pseudomonas putida PP2258 protein or its overexpression results in defective motility on solid media. The PP2258 protein is tripartite, possessing a PAS domain linked to two domains associated with turnover of c-di-GMP - a cyclic nucleotide that controls the switch between motile and sessile lifestyles. Th...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12045

    authors: Österberg S,Åberg A,Herrera Seitz MK,Wolf-Watz M,Shingler V

    更新日期:2013-08-01 00:00:00

  • Isolation and genetic characterization of an improved benzene-tolerant mutant of Pseudomonas putida S12.

    abstract::Pseudomonas putida S12.49, a mutant stain of P. putida S12 that tolerates up to 20 mM benzene, was obtained by evolutionary selection. The genetic basis for the strongly enhanced benzene tolerance was investigated by proteome and transcriptome analysis. Indications were found that the highly benzene-tolerant phenotype...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2010.00172.x

    authors: Volkers RJ,Ballerstedt H,De Winde JH,Ruijssenaars HJ

    更新日期:2010-06-01 00:00:00

  • Virulence and polar tube protein genetic diversity of Nosema ceranae (Microsporidia) field isolates from Northern and Southern Europe in honeybees (Apis mellifera iberiensis).

    abstract::Infection of honeybees by the microsporidian Nosema ceranae is considered to be one of the factors underlying the increased colony losses and decreased honey production seen in recent years. However, these effects appear to differ in function of the climatic zone, the distinct beekeeping practices and the honeybee spe...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12133

    authors: Van der Zee R,Gómez-Moracho T,Pisa L,Sagastume S,García-Palencia P,Maside X,Bartolomé C,Martín-Hernández R,Higes M

    更新日期:2014-08-01 00:00:00

  • Geographically conserved microbiomes of four temperate water tunicates.

    abstract::Tunicates are useful models for exploring microbiomes because they have an innate immune system resembling that of chordates. Automated ribosomal RNA intergenic spacer analysis and High-Throughput Sequencing were used to compare the tunic microbiomes of Ciona robusta (formerly Ciona intestinalis type A), Ciona savigny...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12391

    authors: Cahill PL,Fidler AE,Hopkins GA,Wood SA

    更新日期:2016-08-01 00:00:00

  • Methanotrophic bacteria associated to rice roots: the cultivar effect assessed by T-RFLP and microarray analysis.

    abstract::Rice plants play a key role in regulating methane emissions from paddy fields by affecting both underlying processes: methane production and oxidation. Specific differences were reported for methane oxidation rates; however, studies on the bacterial communities involved are rare. Here, we analysed the methanotrophic c...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2011.00251.x

    authors: Lüke C,Bodrossy L,Lupotto E,Frenzel P

    更新日期:2011-10-01 00:00:00

  • Comparing effective population sizes of dominant marine alphaproteobacteria lineages.

    abstract::A fundamental question in marine microbial ecology is how microbes adapt to ocean environments. Although numerically dominant populations are typically considered more successful, higher census population sizes (Nc) do not equate directly to a greater capability for adaptation. Instead, effective population size (Ne) ...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12129

    authors: Luo H,Swan BK,Stepanauskas R,Hughes AL,Moran MA

    更新日期:2014-04-01 00:00:00

  • Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1.

    abstract::Pyrrolysine (Pyl), the 22nd canonical amino acid, is only decoded and synthesized by a limited number of organisms in the domains Archaea and Bacteria. Pyl is encoded by the amber codon UAG, typically a stop codon. To date, all known Pyl-decoding archaea are able to carry out methylotrophic methanogenesis. The functio...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12545

    authors: Guan Y,Haroon MF,Alam I,Ferry JG,Stingl U

    更新日期:2017-08-01 00:00:00

  • Bacterial diversity associated with freshwater zooplankton.

    abstract::Bacterial community compositions (BCC) associated with the cladoceran Bosmina coregoni and the cyclopoid copepod Thermocyclops oithonoides in oligotrophic Lake Stechlin versus eutrophic Lake Dagow (northeastern Germany) were compared using molecular techniques. We also transplanted the zooplankton from their native la...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2008.00003.x

    authors: Grossart HP,Dziallas C,Tang KW

    更新日期:2009-02-01 00:00:00

  • Assimilation of marine extracellular polymeric substances by deep-sea prokaryotes in the NW Mediterranean Sea.

    abstract::This study examined total uptake of extracellular polymeric substances (EPS) and glucose and the percentage of prokaryotic cells (Bacteria, Crenarchaea and Euryarchaea) consuming these compounds in the major water masses at the DYFAMED site (NW Mediterranean Sea). The potential assimilation rates of EPS at 10 m depth ...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2011.00285.x

    authors: Boutrif M,Garel M,Cottrell MT,Tamburini C

    更新日期:2011-12-01 00:00:00

  • Configuration of redox gradient determines magnetotactic polarity of the marine bacteria MO-1.

    abstract::Magnetotactic bacteria are capable of aligning and swimming along the geomagnetic field lines; such a behaviour is called magnetotaxis. Previous studies reported that bacteria in the northern hemisphere migrate preferentially towards the North Pole of the Earth's magnetic field (north-seeking, NS), whereas those in th...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2010.00150.x

    authors: Zhang WJ,Chen C,Li Y,Song T,Wu LF

    更新日期:2010-10-01 00:00:00

  • Quorum sensing signal production and inhibition by coral-associated vibrios.

    abstract::Corals are inhabited by complex communities of microbes that affect their growth and survival. Several studies suggest that coral disease may be attributed to the success of vibrios in out-competing other bacteria in the mucus and tissues of corals. Vibrios utilize a variety of quorum sensing (QS) signal molecules to ...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2009.00122.x

    authors: Tait K,Hutchison Z,Thompson FL,Munn CB

    更新日期:2010-02-01 00:00:00

  • Deep-sea hydrothermal vent metagenome-assembled genomes provide insight into the phylum Nanoarchaeota.

    abstract::Ectosymbiotic Nanoarchaeota live on the surface of diverse archaeal hosts. Despite being broadly distributed in global geothermal systems, only three Nanoarchaeota have been successfully co-cultivated with their hosts, and until now no nanoarchaeotal cultures or genomes have been described from deep-sea hydrothermal v...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12740

    authors: St John E,Flores GE,Meneghin J,Reysenbach AL

    更新日期:2019-04-01 00:00:00

  • Nematopsis temporariae (Gregarinasina, Apicomplexa, Alveolata) is an intracellular infectious agent of tadpole livers.

    abstract::Amphibians are in decline as a result of habitat destruction, climate change and infectious diseases. Tadpoles are thought susceptible to infections because they are dependent on only an innate immune system (e.g. macrophages). This is because the frog adaptive immune system does not function until later stages of the...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12421

    authors: Chambouvet A,Valigurová A,Pinheiro LM,Richards TA,Jirků M

    更新日期:2016-10-01 00:00:00

  • Interaction of Aspergillus fumigatus conidia with Acanthamoeba castellanii parallels macrophage-fungus interactions.

    abstract::Aspergillus fumigatus and free-living amoebae are common inhabitants of soil. Mechanisms of A. fumigatus to circumvent the amoeba's digestion may facilitate overcoming the vertebrate macrophage defence mechanisms. We performed co-culture experiments using A. fumigatus conidia and the amoeba Acanthamoeba castellanii. A...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12082

    authors: Van Waeyenberghe L,Baré J,Pasmans F,Claeys M,Bert W,Haesebrouck F,Houf K,Martel A

    更新日期:2013-12-01 00:00:00

  • Enigmatic reticulated filaments in subsurface granite.

    abstract::In the last few years, geomicrobiologists have focused their researches on the nature and origin of enigmatic reticulated filaments reported in modern and fossil samples from limestone caves and basalt lava tubes. Researchers have posed questions on these filaments concerning their nature, origin, chemistry, morpholog...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/j.1758-2229.2012.00375.x

    authors: Miller AZ,Hernández-Mariné M,Jurado V,Dionísio A,Barquinha P,Fortunato E,Afonso MJ,Chaminé HI,Saiz-Jimenez C

    更新日期:2012-12-01 00:00:00

  • Arsenic and the gastrointestinal tract microbiome.

    abstract::Arsenic is a toxin, ranking first on the Agency for Toxic Substances and Disease Registry and the Environmental Protection Agency Priority List of Hazardous Substances. Chronic exposure increases the risk of a broad range of human illnesses, most notably cancer; however, there is significant variability in arsenic-ind...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章,评审

    doi:10.1111/1758-2229.12814

    authors: McDermott TR,Stolz JF,Oremland RS

    更新日期:2020-04-01 00:00:00

  • Insights into the genomic plasticity of Pseudomonas putida KF715, a strain with unique biphenyl-utilizing activity and genome instability properties.

    abstract::Pseudomonas putida KF715 exhibits unique properties in both catabolic activity and genome plasticity. Our previous studies revealed that the DNA region containing biphenyl and salycilate metabolism gene clusters (termed the bph-sal element) was frequently deleted and transferred by conjugation to closely related P. pu...

    journal_title:Environmental microbiology reports

    pub_type: 杂志文章

    doi:10.1111/1758-2229.12561

    authors: Suenaga H,Fujihara H,Kimura N,Hirose J,Watanabe T,Futagami T,Goto M,Shimodaira J,Furukawa K

    更新日期:2017-10-01 00:00:00