Abstract:
:Amphibians are in decline as a result of habitat destruction, climate change and infectious diseases. Tadpoles are thought susceptible to infections because they are dependent on only an innate immune system (e.g. macrophages). This is because the frog adaptive immune system does not function until later stages of their life cycle. In 1920, Nöller described a putative infectious agent of tadpoles named Nematopsis temporariae, which he putatively assigned to gregarine protists (Apicomplexa). Here, we identify a gregarine infection of tadpoles using both microscopy and ribosomal DNA sequencing of three different frog species (Rana temporaria, R. dalmatina, and Hyla arborea). We show that this protist lineage belongs to the subclass Gregarinasina Dufour 1828 and is regularly present in macrophages located in liver sinusoids of tadpoles, confirming the only known case of a gregarine infection of a vertebrate.
journal_name
Environ Microbiol Repjournal_title
Environmental microbiology reportsauthors
Chambouvet A,Valigurová A,Pinheiro LM,Richards TA,Jirků Mdoi
10.1111/1758-2229.12421subject
Has Abstractpub_date
2016-10-01 00:00:00pages
675-679issue
5issn
1758-2229journal_volume
8pub_type
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