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Glucosidation of estradiol-17 beta in the cultured ovaries of the silkworm, Bombyx mori.

Abstract：

:Metabolic pathways of estradiol-17 beta and other vertebrate steroid hormones of cultured silkworm pupal ovaries were examined using 14C-labeled steroids. The isolated ovaries showed significant uptake and metabolic activity of the 14C-labeled estradiol-17 beta added to the medium. Analysis of the metabolized compounds by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) revealed extensive metabolic conversion of [14C]estradiol-17 beta and estrone; i.e., estrone was reduced to estradiol-17 beta and estradiol-17 beta was metabolized to conjugates, including estradiol-3-beta-D-glucoside and estradiol-17-alpha-D-glucoside. [14C]Testosterone was not transformed appreciably by the ovaries. Metabolic activity and physiological significance of the vertebrate steroid hormones in the silkworm ovaries are discussed.

journal_name

Gen Comp Endocrinol

journal_title

General and comparative endocrinology

authors

Ogiso M,Fujimoto Y,Ikekawa N,Ohnishi E

doi

10.1016/0016-6480(86)90225-x

subject

Has Abstract

pub_date

1986-03-01 00:00:00

pages

393-401

issue

3

eissn

0016-6480

issn

1095-6840

journal_volume

61

pub_type

杂志文章

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