Structure of Mutant Hepatitis B Core Protein Capsids with Premature Secretion Phenotype.


:Hepatitis B virus is a major human pathogen that consists of a viral genome surrounded by an icosahedrally ordered core protein and a polymorphic, lipidic envelope that is densely packed with surface proteins. A point mutation in the core protein in which a phenylalanine at position 97 is exchanged for a smaller leucine leads to premature envelopment of the capsid before the genome maturation is fully completed. We have used electron cryo-microscopy and image processing to investigate how the point mutation affects the structure of the capsid at 2.6- to 2.8 Å-resolution. We found that in the mutant the smaller side chain at position 97 is displaced, increasing the size of an adjacent pocket in the center of the spikes of the capsid. In the mutant, this pocket is filled with an unknown density. Phosphorylation of serine residues in the unresolved C-terminal domain of the mutant leaves the structure of the ordered capsid largely unchanged. However, we were able to resolve several previously unresolved residues downstream of proline 144 that precede the phosphorylation-sites. These residues pack against the neighboring subunits and increase the inter-dimer contact suggesting that the C-termini play an important role in capsid stabilization and provide a much larger interaction interface than previously observed.


J Mol Biol


Böttcher B,Nassal M




Has Abstract


2018-12-07 00:00:00














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    abstract::The N-terminal SH3 domain of the Drosophila drk protein (drkN SH3) exists in equilibrium between folded and unfolded states under non-denaturing buffer conditions. In order to examine the origins of this instability, we have made mutations in the domain and characterized the thermodynamics and kinetics of folding. Res...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Mok YK,Elisseeva EL,Davidson AR,Forman-Kay JD

    更新日期:2001-03-30 00:00:00

  • Kinetic role of a meta-stable native-like two-disulphide species in the folding transition of bovine pancreatic trypsin inhibitor.

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Creighton TE,Goldenberg DP

    更新日期:1984-11-05 00:00:00

  • Cooperative unfolding of a metastable serpin to a molten globule suggests a link between functional and folding energy landscapes.

    abstract::Alpha-1 antitrypsin (alpha(1)-AT) is a member of the serpin class of protease inhibitors, and folds to a metastable state rather than its thermodynamically most stable native state. Upon cleavage by a target protease, alpha(1)-AT undergoes a dramatic conformational change to a stable form, translocating the bound prot...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Tsutsui Y,Wintrode PL

    更新日期:2007-08-03 00:00:00

  • Structure of alpha-chymotrypsin refined at 1.68 A resolution.

    abstract::Diffraction data for alpha-chymotrypsin crystals at -10 degrees C were measured at 1.68 A resolution and refined by restrained structure-factor least-squares refinement. The two independent chymotrypsin molecules in the crystallographic asymmetric unit were refined independently. The overall structure of alpha-chymotr...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Tsukada H,Blow DM

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  • Positions of proteins S14, S18 and S20 in the 30 S ribosomal subunit of Escherichia coli.

    abstract::A map of the 30 S ribosomal subunit is presented giving the positions of 15 of its 21 proteins. The components located in the map are S1, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, S14, S15, S18 and S20. ...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Ramakrishnan V,Capel M,Kjeldgaard M,Engelman DM,Moore PB

    更新日期:1984-04-05 00:00:00

  • Tryptophan supports interaction of transmembrane helices.

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Ridder A,Skupjen P,Unterreitmeier S,Langosch D

    更新日期:2005-12-09 00:00:00

  • Protein S20 binds two 16S rRNA sites as assembly is initiated.

    abstract::Ribosomal protein S20 is a primary binding protein that bridges the 5' domain and the 3' minor domain of the 16S ribosomal RNA (rRNA) in the 30S ribosomal subunit. Using time-dependent dimethyl sulfate modification, we have determined that as it is bound to 16S rRNA, protein S20 causes rapid protection of bases A246, ...

    journal_title:Journal of molecular biology

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    authors: Hedrick EG,Hill WE

    更新日期:2010-08-20 00:00:00

  • Predicting Protein-Protein Interfaces that Bind Intrinsically Disordered Protein Regions.

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


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  • The nucleolar PICT-1/GLTSCR2 protein forms homo-oligomers.

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    authors: Borodianskiy-Shteinberg T,Kalt I,Kipper S,Nachum N,Katz S,Pauker MH,Barda-Saad M,Gerber D,Sarid R

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  • Effects of Mg2+, K+, and H+ on an equilibrium between alternative conformations of an RNA pseudoknot.

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    journal_title:Journal of molecular biology

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    authors: Johs A,Harwood IM,Parks JM,Nauss RE,Smith JC,Liang L,Miller SM

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  • Assignment of the 1H nuclear magnetic resonance spectrum of the proteinase inhibitor IIA from bull seminal plasma by two-dimensional nuclear magnetic resonance at 500 MHz.

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


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    journal_title:Journal of molecular biology

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  • β-Lactamases and β-Lactamase Inhibitors in the 21st Century.

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    journal_title:Journal of molecular biology

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    authors: Sharma D,Putnam AA,Jankowsky E

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    journal_title:Journal of molecular biology

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  • A branched pathway in the early stage of transcription by Escherichia coli RNA polymerase.

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    journal_title:Journal of molecular biology

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  • The crystal and molecular structure of the Rhizomucor miehei triacylglyceride lipase at 1.9 A resolution.

    abstract::The crystal and molecular structure of a triacylglyceride lipase (EC from the fungus Rhizomucor miehei was analyzed using X-ray single crystal diffraction data to 1.9 A resolution. The structure was refined to an R-factor of 0.169 for all available data. The details of the molecular architecture and the cryst...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Derewenda ZS,Derewenda U,Dodson GG

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  • A novel calmodulin-like gene from the nematode Caenorhabditis elegans.

    abstract::A novel gene from the nematode Caenorhabditis elegans was isolated by hybridization with a human calmodulin complementary DNA probe. This gene, cal-1, is present at one copy per haploid genome. In-situ hybridization of the cloned gene to metaphase chromosomes allowed us to assign it to the nematode linkage group IV. T...

    journal_title:Journal of molecular biology

    pub_type: 杂志文章


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  • Order-disorder-order transitions mediate the activation of cholera toxin.

    abstract::Cholera toxin (CT) holotoxin must be activated to intoxicate host cells. This process requires the intracellular dissociation of the enzymatic CTA1 domain from the holotoxin components CTA2 and B5, followed by subsequent interaction with the host factor ADP ribosylation factor 6 (ARF6)-GTP. We report the first NMR-bas...

    journal_title:Journal of molecular biology

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    authors: Ampapathi RS,Creath AL,Lou DI,Craft JW Jr,Blanke SR,Legge GB

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  • Structure of short thick filaments from Limulus muscle.

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    journal_title:Journal of molecular biology

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    journal_title:Journal of molecular biology

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    authors: Gu J,Villanueva RA,Snyder CS,Roth MJ,Georgiadis MM

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    journal_title:Journal of molecular biology

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    authors: Moon TM,Correa F,Kinch LN,Piala AT,Gardner KH,Goldsmith EJ

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Pagel JM,Winkelman JW,Adams CW,Hatfield GW

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  • Crystal structure at 1.95 A resolution of the breast tumour-specific antibody SM3 complexed with its peptide epitope reveals novel hypervariable loop recognition.

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Dokurno P,Bates PA,Band HA,Stewart LM,Lally JM,Burchell JM,Taylor-Papadimitriou J,Snary D,Sternberg MJ,Freemont PS

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  • Identification of three aspartic acid residues essential for catalysis by the RusA holliday junction resolvase.

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Bolt EL,Sharples GJ,Lloyd RG

    更新日期:1999-02-19 00:00:00

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Kolesky S,Ouhammouch M,Brody EN,Geiduschek EP

    更新日期:1999-08-13 00:00:00

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    journal_title:Journal of molecular biology

    pub_type: 杂志文章


    authors: Meijer PJ,Andersen PS,Haahr Hansen M,Steinaa L,Jensen A,Lantto J,Oleksiewicz MB,Tengbjerg K,Poulsen TR,Coljee VW,Bregenholt S,Haurum JS,Nielsen LS

    更新日期:2006-05-05 00:00:00