Abstract:
AIMS:Most benign breast fibroepithelial lesions (FEL) in adults harbour recurrent somatic MED12 exon 2 mutations and rare TERT promoter hotspot mutations. We sought to determine the frequency of MED12 exon 2 and TERT promoter hotspot mutations in fibroadenomas (FA) and benign phyllodes tumours (BePT) in adolescents and young adults. METHODS:DNA from 21 consecutive FAs and eight consecutive BePTs in adolescents and young adults was subjected to Sanger sequencing of the exon 2 of MED12 and the TERT promoter hotspot locus. RESULTS:We identified MED12 exon 2 mutations in 62% and 88% of FAs and BePTs, respectively, and no TERT promoter hotspot mutations. The majority of the MED12 exon 2 mutations identified were in-frame deletions (60%). CONCLUSIONS:As in adults, benign FELs in juvenile patients harbour recurrent MED12 exon 2 mutations.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Pareja F,Da Cruz Paula A,Murray MP,Hoang T,Gularte-Mérida R,Brown D,da Silva EM,Sebastiao APM,Giri DD,Weigelt B,Reis-Filho JS,Brogi Edoi
10.1136/jclinpath-2018-205570subject
Has Abstractpub_date
2019-03-01 00:00:00pages
258-262issue
3eissn
0021-9746issn
1472-4146pii
jclinpath-2018-205570journal_volume
72pub_type
杂志文章abstract:AIMS:To evaluate a recently developed digital slide and virtual microscope system, and to compare this method with optical microscopy on routine gastrointestinal biopsy specimens in both local and remote access modes. METHODS:A fully computer controlled commercial microscope was used. The scanning program included obj...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.56.6.433
更新日期:2003-06-01 00:00:00
abstract:AIMS:To investigate staining patterns for mannan binding protein (MBP) by immunocytochemistry in liver biopsy specimens from patients with various hepatic disorders; to measure the serum MBP concentration in the patients at the time of biopsy; and to compare these to define further the role of MBP in disease. METHODS:...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
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abstract:AIMS:To show that medullary carcinomas of the thyroid are morphologically indistinguishable from gut carcinoids: the value of histochemistry in their identification and differential diagnosis from metastatic carcinoid tumours to the thyroid and some follicular cell neoplasms. METHODS:15 thyroid medullary carcinomas wi...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.46.2.113
更新日期:1993-02-01 00:00:00
abstract::The isotopic estimation of the cortisol secretion rate in man is now an accepted and reliable method of assaying adrenal cortical function.Seven years' experience with its clinical use is reviewed and some practical aspects of technique are considered. The mean normal resting cortisol secretion rate is 16.2 +/- 5.7 mg...
journal_title:Journal of clinical pathology
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doi:10.1136/jcp.18.1.82
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journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jcp.2005.033217
更新日期:2007-06-01 00:00:00
abstract::Incomplete expression of human haemoglobin beta-chains at birth may lead to difficulty in the early demonstration of an inherited beta-chain variant. In this case, the rare unstable variant, Hb Tacoma beta30 (B12) arg leads to ser, although not present in cord blood in sufficient amounts to be easily detected by routi...
journal_title:Journal of clinical pathology
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abstract::The cellularity of 104 primary breast carcinomata was determined by semiautomated image analysis to allow the relation between cellularity and oestrogen receptor content values to be assessed. The oestrogen receptor content of tumours detected by a steroid binding assay showed no correlation with cellularity, although...
journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2004.019299
更新日期:2005-03-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-204103
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journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
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更新日期:1981-05-01 00:00:00
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journal_title:Journal of clinical pathology
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doi:10.1136/jclinpath-2020-206629
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abstract::During a 12-month period, 40 146 blood donors were tested for the presence of high titre antitetanus antibodies. An attempt has been made to define the most suitable donors, those with antitetanus antibody titres of 4 IU/ml or above, by virtue of titre, sex, location, and length of time since most recent immunisation...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.2.177
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2015-203580
更新日期:2016-09-01 00:00:00
abstract::A spectrophotometric assay of proteolytic enzyme activity is described in which the optical density obtained by a given enzyme preparation is related (2 alpha less than 0.01) to the serological activity of that preparation in a two stage technique. The assay appears to be of use for the standardisation of enzyme prepa...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.3.329
更新日期:1984-03-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jcp.2007.049312
更新日期:2008-01-01 00:00:00
abstract::A technique is described whereby inclusion-bearing cells identified by light microscopy in stained smears of urinary sediment were reprocessed for examination in the electron microscope. The nuclei of the abnormal cells were found to contain numerous virus particles, 35 nm in diameter, which morphologically resembled ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.30.11.1015
更新日期:1977-11-01 00:00:00
abstract::A new staining method was developed for the detection of inclusions of Chlamydia psittaci and Chlamydia trachomatis inclusions in cell cultures. Using a combination of methyl green and neutral red stains and washing at pH 5.0, inclusions were stained red while cell cytoplasm was pale pink and cell nuclei were pale gre...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.35.6.642
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
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journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
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abstract::The distribution and quantitative expression of HBcAg in relation to serum HBeAg and liver histology before and after a trial of interferon in 50 patients with chronic type B hepatitis were evaluated using polyclonal and monoclonal antibodies. In general, both antisera showed a similar pattern in terms of the distribu...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章
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journal_title:Journal of clinical pathology
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journal_title:Journal of clinical pathology
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更新日期:2003-05-01 00:00:00
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.4.383
更新日期:1989-04-01 00:00:00
abstract::The activities of three commercial products of papain were compared as supplied and after activation, and when used in the enzyme-serum method for rhesus (D) grouping. Certain dangers in activation became apparent, and accurate determination of the proportions of papain to anti-D appeared more important than indiscrim...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.15.6.503
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journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.38.11.1304
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journal_title:Journal of clinical pathology
pub_type: 杂志文章,评审
doi:10.1136/jclinpath-2012-201091
更新日期:2013-03-01 00:00:00
abstract:AIMS:This cross-sectional study investigated the association between eight herpesviruses and the bacterial community profiles from the oral cavity of children with and without leukaemia. METHODS:Sixty participants (aged 3-13), divided into the leukaemia group (LG) and healthy group (HG), were evaluated. Collection of ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202668
更新日期:2015-03-01 00:00:00